Origin IGS: tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagtaaaacagaaagcggatatgtcggttatggcatatccgcttttgctttatagctctttatttaaccactatttgcattgtttagaaaggcaagctatctcccttgataaataacgaaaagtctttgggagataacattgtttcggatttttctgtgctttcctttataattagatatgtgtagaatcatcaaaaagttttatctgaagaattatttacgtgtaaagaaatatttatttacacgtatggaagaatttatttacacgtagaaaaatatttctttacgtgtaaataatttggtagtgttgctttaaaatggctcttcttcttcattaactaattcagtagacgcatcttttatggtcatacggaattgatagatacaagccagaagatgatgttgaaaaagtagattcctgttcctttcatttttagttctaattaaacattcacattcttttggttatttagttttttgtatctttgcctttgatatgcacaaaggtaaaaacaattatacattccttaccgaggagcctattcataaggttgtgataaca Mask Tandem Repeat Region ================================================ tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Find is-nt database================================================ Query_seq: PI1417:PI1418|PI1417:PI1418:MATE efflux family protein (Na+-driven multidrug efflux pump):UDP-N-acetylglucosamine 2-epimerase:->->:1337562..1338124 563 tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1417:PI1418|PI1417:PI1418:MATE efflux family protein (Na+-driven multidrug efflux pump):UDP-N-acetylglucosamine 2-epimerase:->->:1337562..1338124 563 tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1417:PI1418|PI1417:PI1418:MATE efflux family protein (Na+-driven multidrug efflux pump):UDP-N-acetylglucosamine 2-epimerase:->->:1337562..1338124 563 tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 119 End: 247 ...................................................................................................................... M R T K N E R N R N L L F Q H H L L A C I Y Q F R M T I K D A S T E L V N E E E E P F ............................................................................................................................................................................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 368 End: 463 ............................................................................................................................................................................................................................................................................................................................................................................... M M I L H I S N Y K G K H R K I R N N V I S Q R L F V I Y Q G R .................................................................................................... Protein_Len: 31 Strand: - Start: 450 End: 542 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................. K D L S I A Q R E L C H L Y H N F L S S Y L L L P Y A M V S M ..................... Protein_Len: 30 Strand: - Start: 295 End: 384 ...................................................................................................................................................................................................................................................................................................... T Y I F E E M R T F L Y K K V R L Y N K L Y F K K I I R C M ................................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 1 End: 144 T I V V K H I P E E T L F T Y N N K G K H M D F A F I C F V L Y G F S H S H K I L V L F S L F M ................................................................................................................................................................................................................................................................................................................................................................................................................................... tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 374 End: 403 .....................................................................................................................................................................................................................................................................................................................................................................................CTTTCCTTTATAATTAGATATGTGTAGAAT................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 390 End: 419 .....................................................................................................................................................................................................................................................................................................................................................................................................TTCGGATTTTTCTGTGCTTTCCTTTATAAT................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 74 HP_score: -8.3 Tail_Score: -3.68836 Start: 265 End: 306 Full_Region: AAGCAACACTACCAA ATTATTTACACGTAAAGAA ATAT TTTTCTACGTGTAAATAAA TTCTTCCATACGTGT ........................................................................................................................................................................................................................................................................ATTATTTACACGTAAAGAAATATTTTTCTACGTGTAAATAAA................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 89 HP_score: -11.8 Tail_Score: -2.8082 Start: 266 End: 305 Full_Region: AGCAACACTACCAAA TTATTTACACGTAAAGAA ATAT TTTTCTACGTGTAAATAA ATTCTTCCATACGTG .........................................................................................................................................................................................................................................................................TTATTTACACGTAAAGAAATATTTTTCTACGTGTAAATAA.................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 95 HP_score: -11.8 Tail_Score: -3.8505 Start: 266 End: 305 Full_Region: cacgtatggaagaat ttatttacacgtagaaaa atat ttctttacgtgtaaataa tttggtagtgttgct .........................................................................................................................................................................................................................................................................ttatttacacgtagaaaaatatttctttacgtgtaaataa.................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 69 HP_score: -7.6 Tail_Score: -3.28888 Start: 522 End: 547 Full_Region: GCTATAAAGCAAAAG CGGATATGCC ATAACC GACATATCCG CTTTCTGTTTTACTT .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGGATATGCCATAACCGACATATCCG................ Find igs database================================================ Query_seq: PI1417:PI1418|PI1417:PI1418:MATE efflux family protein (Na+-driven multidrug efflux pump):UDP-N-acetylglucosamine 2-epimerase:->->:1337562..1338124 563 tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Intra-Species Hit: Count: 1 Min: 1 Max: 563 Len: 563 Subject: pint_PI1417_PI1418|MATE efflux family protein (Na+-driven multidrug efflux pump):UDP-N-acetylglucosamine 2-epimerase|POSITIVE:POSITIVE|[1337562,1338124]|563 HSP 1 e-value: 0.0 bit: 1116.0 Len: 563 Query Start:1 Query End:563 Subject Strand: POSITIVE Subject Start: 1 Subject End: 563 tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta tgttatcacaaccttatgaataggctcctcggtaaggaatgtataattgtttttacctttgtgcatatcaaaggcaaagatacaaaaaactaaataaccaaaagaatgtgaatgtttaattagaactaaaaatgaaaggaacaggaatctactttttcaacatcatcttctggcttgtatctatcaattccgtatgaccataaaagatgcgtctactgaattagttaatgaagaagaagagccattttaaagcaacactaccaaattatttacacgtaaagaaatatttttctacgtgtaaataaattcttccatacgtgtaaataaatatttctttacacgtaaataattcttcagataaaactttttgatgattctacacatatctaattataaaggaaagcacagaaaaatccgaaacaatgttatctcccaaagacttttcgttatttatcaagggagatagcttgcctttctaaacaatgcaaatagtggttaaataaagagctataaagcaaaagcggatatgccataaccgacatatccgctttctgttttactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUUAUCACAACCUUAUGAAUAGGCUCCUCGGUAAGGAAUGUAUAAUUGUUUUUACCUUUGUGCAUAUCAAAGGCAAAGAUACAAAAAACUAAAUAACCAAAAGAAUGUGAAUGUUUAAUUAGAACUAAAAAUGAAAGGAACAGGAAUCUACUUUUUCAACAUCAUCUUCUGGCUUGUAUCUAUCAAUUCCGUAUGACCAUAAAAGAUGCGUCUACUGAAUUAGUUAAUGAAGAAGAAGAGCCAUUUUAAAGCAACACUACCAAAUUAUUUACACGUAAAGAAAUAUUUUUCUACGUGUAAAUAAAUUCUUCCAUACGUGUAAAUAAAUAUUUCUUUACACGUAAAUAAUUCUUCAGAUAAAACUUUUUGAUGAUUCUACACAUAUCUAAUUAUAAAGGAAAGCACAGAAAAAUCCGAAACAAUGUUAUCUCCCAAAGACUUUUCGUUAUUUAUCAAGGGAGAUAGCUUGCCUUUCUAAACAAUGCAAAUAGUGGUUAAAUAAAGAGCUAUAAAGCAAAAGCGGAUAUGCCAUAACCGACAUAUCCGCUUUCUGUUUUACUUA .(((((.(((.((((((....((....))..))))))..))))))))(((((((.((((((.......)))))).)))))))............((((((......(((...((((((...............((((((....(((......((((((.......((((((..(((.(((..((.(((((.(((.((((((.....))).)))))).))))).))..)))))).))))))...........((............((((((((((((..(((((....)))))))))))))))))....((((.((((((((((.((....)).))))))))))......((.(((((........))))).)).......................)))).))...)))))))))....(((.((((((((((.......................)))))))))))))))))))))))))..))).....))))))............(((((((((((((((((((.(......).)))))))))))).))))))).... (-130.36) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUAAAACAGAAAGCGGAUAUGUCGGUUAUGGCAUAUCCGCUUUUGCUUUAUAGCUCUUUAUUUAACCACUAUUUGCAUUGUUUAGAAAGGCAAGCUAUCUCCCUUGAUAAAUAACGAAAAGUCUUUGGGAGAUAACAUUGUUUCGGAUUUUUCUGUGCUUUCCUUUAUAAUUAGAUAUGUGUAGAAUCAUCAAAAAGUUUUAUCUGAAGAAUUAUUUACGUGUAAAGAAAUAUUUAUUUACACGUAUGGAAGAAUUUAUUUACACGUAGAAAAAUAUUUCUUUACGUGUAAAUAAUUUGGUAGUGUUGCUUUAAAAUGGCUCUUCUUCUUCAUUAACUAAUUCAGUAGACGCAUCUUUUAUGGUCAUACGGAAUUGAUAGAUACAAGCCAGAAGAUGAUGUUGAAAAAGUAGAUUCCUGUUCCUUUCAUUUUUAGUUCUAAUUAAACAUUCACAUUCUUUUGGUUAUUUAGUUUUUUGUAUCUUUGCCUUUGAUAUGCACAAAGGUAAAAACAAUUAUACAUUCCUUACCGAGGAGCCUAUUCAUAAGGUUGUGAUAACA ...(((((...((((((((((((((((....))))))))))))))))..)))))......((((((((((....(((((((((((((((((((.((((((((((((..(((............)))...)))))))).........((((....)))).)))).)))))....))))))).)))))))........(((((..((.(((..(((((((((((((((((((((((((((.(((((..(((..............)))..))))).)))))))))))))))))))))))))))..)))))..))))).....((((((((............((((((.((((((.(((.....)))))).))).))))))..(((((((((((((((((.((((((.......((((...(((.............))).))))....)))))).....)))))))))...........))))))))(((((((((.......)))))))))......................)))))))).........))))).))))).. (-153.96) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 478 308 262 123 77 pint:PI0648|5end_hypothetical_protein_589448..589678_POSITIVE 458 307 264 100 57 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 458 300 251 115 64 pint:PI0130|5end_hypothetical_protein_122032..122262_POSITIVE 445 305 262 165 122 pint:PI0115|5end_hypothetical_protein_108062..108292_POSITIVE 445 305 262 151 108 pint:PI0111|5end_hypothetical_protein_108048..108278_POSITIVE 445 305 262 116 73 pint:PI0112|5end_hypothetical_protein_108013..108243_POSITIVE 418 340 293 107 60 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 407 307 262 134 89 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 399 307 261 122 76 pint:PI0579|5end_hypothetical_protein_523419..523649_POSITIVE 388 300 251 228 180 pint:PI0599|5end_hypothetical_protein_547376..547606_POSITIVE 382 300 252 206 159 pint:PI0702|5end_conserved_hypothetical_protein;_possible_tetracenomycin_C_synthesis_protein_homolog_638640..638870_POSITIVE 379 300 258 93 51 pint:PI1462|5end_hypothetical_protein_1385094..1385324_POSITIVE 345 305 266 157 118 pint:PI0983|5end_hypothetical_protein_909750..909980_POSITIVE 341 300 251 210 161 pint:PI0173|5end_hypothetical_protein_165305..165535_POSITIVE 328 301 262 116 77 pint:PI0535|5end_hypothetical_protein_489508..489738_POSITIVE 326 334 293 231 190 pint:PI0311|5end_hypothetical_protein_294721..294951_POSITIVE 317 305 266 164 125 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 310 557 521 180 143 pint:PI1669|5end_hypothetical_protein_1581257..1581487_POSITIVE 310 57 13 114 66 pint:PI0931|5end_hypothetical_protein_862652..862882_POSITIVE 307 553 514 215 182 pint:PI0251|5end_hypothetical_protein_247551..247781_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 399 355 316 50 11 pint:PI0808|3end_ISPg3-related_transposase_746457..746607_POSITIVE 394 339 293 113 67 pint:PI2105|3end_hypothetical_protein_2059586..2059736_POSITIVE 392 360 311 65 16 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 378 301 263 50 12 pint:PI1314|3end_hypothetical_protein_1231423..1231573_POSITIVE 352 550 507 57 9 pint:PI0487|3end_50S_ribosomal_protein_L17_446703..446853_POSITIVE 341 300 251 63 14 pint:PI0172|3end_ISPg3-related_transposase_165238..165388_POSITIVE 317 351 315 43 7 pint:PI1461|3end_conserved_hypothetical_protein_1385073..1385223_POSITIVE 286 351 315 74 38 pint:PI0533|3end_ABC_transporter,_permease_protein_488421..488571_POSITIVE 284 230 184 127 81 pint:PI1673|3end_hypothetical_protein_1587902..1588052_POSITIVE 280 69 21 100 46 pint:PI0787|3end_glycosyltransferase_725793..725943_POSITIVE 279 54 14 151 107 pint:PI1598|3end_phosphate_ABC_transporter,_phosphate-binding_component_1527682..1527832_POSITIVE 273 344 301 100 57 pint:PI0725|3end_hypothetical_protein_658933..659083_POSITIVE 269 547 515 71 45 pint:PI0287|3end_ISPg3-related_transposase_272352..272502_POSITIVE 266 467 421 128 89 pint:PI0513|3end_ion_transporter_472371..472521_POSITIVE 264 349 315 50 16 pint:PI0797|3end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735752..735902_POSITIVE 258 530 483 134 81 pint:PI0830|3end_conserved_hypothetical_protein_768206..768356_POSITIVE 256 551 515 150 113 pint:PI0755|3end_hypothetical_protein_686884..687034_POSITIVE 256 558 512 113 71 pint:PI0565|3end_hypothetical_protein_511657..511807_POSITIVE 255 540 492 112 59 pint:PI2041|3end_conserved_hypothetical_protein_1994660..1994810_POSITIVE 255 467 424 89 48 pint:PI1883|3end_conserved_hypothetical_protein;_possible_surface_protein_1826174..1826324_POSITIVE