Origin IGS: taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacatcgaaatctttcgcttcgttttgtacttttacttcttctttggtttcttctttgctaccaaatagtttctgaaaaaagttcatacgtgtttatgtttatttatattcaacgaaggaactaaccatcgtcttcaattcattcgttatttataatatgcaaacttacatatttttttcgacatatcaacctgttta Mask Tandem Repeat Region ================================================ taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Find is-nt database================================================ Query_seq: PI1400:PI1401|PI1400:PI1401:hypothetical protein:TPR-repeat-containing protein:->->:1321969..1322166 198 taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1400:PI1401|PI1400:PI1401:hypothetical protein:TPR-repeat-containing protein:->->:1321969..1322166 198 taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1400:PI1401|PI1400:PI1401:hypothetical protein:TPR-repeat-containing protein:->->:1321969..1322166 198 taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 3 End: 113 .. V P Q Y T S F F I Y T Q M N Y I V F S N F V I T L E K T S Y L Y V Y V R M ..................................................................................... taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 42 HP_score: -2.6 Tail_Score: -3.1355 Start: 68 End: 85 Full_Region: CGAATGAATTGAAGA CGATGG TTAGTT CCTTCG TTGAATATAAATAAA ...................................................................CGATGGTTAGTTCCTTCG................................................................................................................. TransTerm Strand: - Conf: 43 HP_score: -2.6 Tail_Score: -3.18134 Start: 68 End: 85 Full_Region: tttatttatattcaa cgaagg aactaa ccatcg tcttcaattcattcg ...................................................................cgaaggaactaaccatcg................................................................................................................. Find igs database================================================ Query_seq: PI1400:PI1401|PI1400:PI1401:hypothetical protein:TPR-repeat-containing protein:->->:1321969..1322166 198 taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Intra-Species Hit: Count: 1 Min: 1 Max: 198 Len: 198 Subject: pint_PI1400_PI1401|hypothetical protein:TPR-repeat-containing protein|POSITIVE:POSITIVE|[1321969,1322166]|198 HSP 1 e-value: 6.0E-96 bit: 351.0 Len: 198 Query Start:1 Query End:198 Subject Strand: POSITIVE Subject Start: 1 Subject End: 198 taaacaggttgatatgtcgnnnnnnntatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta taaacaggttgatatgtcgaaaaaaatatgtaagtttgcatattataaataacgaatgaattgaagacgatggttagttccttcgttgaatataaataaacataaacacgtatgaacttttttcagaaactatttggtagcaaagaagaaaccaaagaagaagtaaaagtacaaaacgaagcgaaagatttcgatgta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACAGGUUGAUAUGUCGAAAAAAAUAUGUAAGUUUGCAUAUUAUAAAUAACGAAUGAAUUGAAGACGAUGGUUAGUUCCUUCGUUGAAUAUAAAUAAACAUAAACACGUAUGAACUUUUUUCAGAAACUAUUUGGUAGCAAAGAAGAAACCAAAGAAGAAGUAAAAGUACAAAACGAAGCGAAAGAUUUCGAUGUA .....(((((.((((((.(.....((((((((....)))))))).....(((((((.(((((((.........))))))).)))))))..................))))))).)))))..........((.((((((..(......)..))))))..)).........((((...((((((....).))))).)))) (-37.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACAUCGAAAUCUUUCGCUUCGUUUUGUACUUUUACUUCUUCUUUGGUUUCUUCUUUGCUACCAAAUAGUUUCUGAAAAAAGUUCAUACGUGUUUAUGUUUAUUUAUAUUCAACGAAGGAACUAACCAUCGUCUUCAAUUCAUUCGUUAUUUAUAAUAUGCAAACUUACAUAUUUUUUUCGACAUAUCAACCUGUUUA ....(((((((((((((...(((..((.((((((..(((..(((((((..(......)..)))))..)).....))))))))).)).))).(..((((......))))..)..)))))))..................................((((((........))))))..))))))................ (-24.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 380 166 121 94 51 pint:PI0760|5end_ATP-dependent_RNA_helicase,_DEAD/DEAH_box_family_694086..694316_POSITIVE 273 169 124 76 24 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 257 87 49 183 147 pint:PI1273|5end_anthranilate_synthase_component_II_1192285..1192515_POSITIVE 252 166 135 91 54 pint:PI0936|5end_conserved_hypothetical_protein_868329..868559_POSITIVE 249 182 141 109 71 pint:PI2033|5end_hypothetical_protein_1987068..1987298_POSITIVE 244 179 132 79 29 pint:PI0374|5end_MbpB_protein_344571..344801_POSITIVE 240 90 62 155 131 pint:PI1276|5end_hypothetical_protein_1194266..1194496_POSITIVE 239 163 121 120 85 pint:PI1956|5end_conserved_hypothetical_protein_1913049..1913279_POSITIVE 233 89 53 48 15 pint:PI0769|5end_phosphomannomutase_704653..704883_POSITIVE 228 185 141 148 95 pint:PI1540|5end_hypothetical_protein_1461362..1461592_POSITIVE 223 85 48 205 163 pint:PI0704|5end_conserved_hypothetical_protein_640247..640477_POSITIVE 218 141 102 147 105 pint:PI0696|5end_NADH_pyrophosphatase,_MutT_family_hydrolase_(NAD+_diphosphatase)_632741..632971_POSITIVE 218 190 147 163 120 pint:PI0276|5end_anti-restriction_protein_264429..264659_POSITIVE 217 179 134 110 56 pint:PI0765|5end_conserved_hypothetical_protein;_possible_surface_protein_699753..699983_POSITIVE 213 179 134 69 35 pint:PI0012|5end_conserved_hypothetical_protein_13406..13636_POSITIVE 212 180 134 70 26 pint:PI1562|5end_methyltransferase_1479644..1479874_POSITIVE 211 88 51 179 143 pint:PI1299|5end_glycosyltransferase_1212191..1212421_POSITIVE 210 180 135 153 100 pint:PI1490|5end_GTP_cyclohydrolase_I_1408571..1408801_POSITIVE 210 172 136 181 146 pint:PI0116|5end_conserved_hypothetical_protein_110213..110443_POSITIVE 206 155 121 185 152 pint:PI2119|5end_conserved_hypothetical_protein;_possible_cytochrome_B_subunit_2068031..2068261_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 271 163 124 149 100 pint:PI1541|3end_hypothetical_protein_1461884..1462034_POSITIVE 249 182 141 82 44 pint:PI2032|3end_RNA_polymerase_sigma_factor,_ECF_subfamily_1987121..1987271_POSITIVE 240 90 62 116 92 pint:PI1275|3end_indole-3-glycerol_phosphate_synthase_1194307..1194457_POSITIVE 239 163 121 49 14 pint:PI1955|3end_N_utilization_substance_protein_A_1913058..1913208_POSITIVE 223 85 48 74 32 pint:PI0703|3end_hypothetical_protein_640196..640346_POSITIVE 218 190 147 143 100 pint:PI0275|3end_hypothetical_protein_264489..264639_POSITIVE 217 173 138 106 71 pint:PI1675|3end_TonB-dependent_outer_membrane_receptor_1590535..1590685_POSITIVE 213 184 151 130 105 pint:PI0918|3end_Fe-S_oxidoreductase,_family_2_849236..849386_POSITIVE 212 180 134 48 4 pint:PI1561|3end_conserved_hypothetical_protein;_possible_lipoprotein_1479702..1479852_POSITIVE 210 150 107 53 1 pint:PI1947|3end_hypothetical_protein_1902865..1903015_POSITIVE 210 180 135 89 36 pint:PI1489|3end_exodeoxyribonuclease_III_1408587..1408737_POSITIVE 207 88 45 115 71 pint:PI0578|3end_methyltransferase_523253..523403_POSITIVE 206 155 121 71 38 pint:PI2118|3end_fumarate_reductase/succinate_dehydrogenase_flavoprotein_subunit,_N-terminal_2067997..2068147_POSITIVE 206 86 55 51 15 pint:PI1407|3end_conserved_hypothetical_protein_1329116..1329266_POSITIVE 205 189 143 117 58 pint:PI1758|3end_hypothetical_protein_1684057..1684207_POSITIVE 205 150 107 38 1 pint:PI1540|3end_hypothetical_protein_1461771..1461921_POSITIVE 204 95 51 55 13 pint:PI1312|3end_conserved_hypothetical_protein_1229677..1229827_POSITIVE 204 126 81 142 102 pint:PI0159|3end_hypothetical_protein_152120..152270_POSITIVE 201 87 52 79 32 pint:PI0548|3end_possible_acetylxylan_esterase_500571..500721_POSITIVE 200 85 47 115 75 pint:PI1426|3end_flavodoxin_1345446..1345596_POSITIVE