Origin IGS: tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataagcaaaacagtattggcaacaccatgttactgtttgttgtaagcagaaatggaaggcgactttataaaaatacacaaaggactgttacctctaagctggctttatgggttaggggta Mask Tandem Repeat Region ================================================ tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Find is-nt database================================================ Query_seq: PI1374:PI1375|PI1374:PI1375:tetraacyldisaccharide 4'-kinase:protease IV family, clan S (peptidase family S49):->->:1295981..1296102 122 tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1374:PI1375|PI1374:PI1375:tetraacyldisaccharide 4'-kinase:protease IV family, clan S (peptidase family S49):->->:1295981..1296102 122 tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1374:PI1375|PI1374:PI1375:tetraacyldisaccharide 4'-kinase:protease IV family, clan S (peptidase family S49):->->:1295981..1296102 122 tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 2 End: 106 . G R V W L A L K S T V T R Q T N K Y L R R G N R S V V F L L M T N G M ................ tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1374:PI1375|PI1374:PI1375:tetraacyldisaccharide 4'-kinase:protease IV family, clan S (peptidase family S49):->->:1295981..1296102 122 tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Intra-Species Hit: Count: 1 Min: 1 Max: 122 Len: 122 Subject: pint_PI1374_PI1375|tetraacyldisaccharide 4'-kinase:protease IV family, clan S (peptidase family S49)|POSITIVE:POSITIVE|[1295981,1296102]|122 HSP 1 e-value: 2.0E-63 bit: 242.0 Len: 122 Query Start:1 Query End:122 Subject Strand: POSITIVE Subject Start: 1 Subject End: 122 tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta tacccctaacccataaagccagcttagaggtaacagtcctttgtgtatttttataaagtcgccttccatttctgcttacaacaaacagtaacatggtgttgccaatactgttttgcttatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACCCCUAACCCAUAAAGCCAGCUUAGAGGUAACAGUCCUUUGUGUAUUUUUAUAAAGUCGCCUUCCAUUUCUGCUUACAACAAACAGUAACAUGGUGUUGCCAAUACUGUUUUGCUUAUUA ...............((((.(((..(((((.....(..(((((((......))))))).).....)).)))..)))......((((((((...(((.....))).)))))))).)))).... (-20.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAAGCAAAACAGUAUUGGCAACACCAUGUUACUGUUUGUUGUAAGCAGAAAUGGAAGGCGACUUUAUAAAAAUACACAAAGGACUGUUACCUCUAAGCUGGCUUUAUGGGUUAGGGGUA .....((((((.(((((.(((.....)))...)))))))))))...(((((.....((((....)))).........(.....)..)))))((((((((.(((......))).)))))))). (-28.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 311 117 74 183 127 pint:PI0993|5end_probable_TPR-repeat-containing_protein_917175..917405_POSITIVE 300 114 87 60 33 pint:PI0647|5end_potassium_uptake_protein_587505..587735_POSITIVE 283 100 58 169 126 pint:PI2045|5end_hypothetical_protein_1998494..1998724_POSITIVE 282 50 6 122 75 pint:PI0830|5end_conserved_hypothetical_protein_766576..766806_POSITIVE 281 65 22 94 57 pint:PI0770|5end_hypothetical_protein_706170..706400_POSITIVE 270 39 1 59 16 pint:PI1197|5end_pseudouridylate_synthetase_1113558..1113788_POSITIVE 269 73 35 231 196 pint:PI0309|5end_hypothetical_protein_291503..291733_POSITIVE 261 116 71 214 166 pint:PI1811|5end_conserved_hypothetical_protein_1749518..1749748_POSITIVE 256 47 11 140 102 pint:PI1863|5end_phosphoribosylformylglycinamidine_synthase_1806674..1806904_POSITIVE 251 67 22 134 86 pint:PI0580|5end_Holliday_junction_DNA_helicase_524178..524408_POSITIVE 247 52 11 103 64 pint:PI0207|5end_conserved_hypothetical_protein_199797..200027_POSITIVE 246 53 12 65 25 pint:PI0460|5end_50S_ribosomal_protein_L3_432471..432701_POSITIVE 244 107 61 171 125 pint:PI1516|5end_multidrug_resistance_protein;_HlyD_family_secretion_protein_1434381..1434611_POSITIVE 243 116 72 175 118 pint:PI0790|5end_hypothetical_protein_728953..729183_POSITIVE 242 80 32 228 180 pint:PI1931|5end_alpha_glycan_phosphorylase,_maltodextrin_phosphorylase_1875456..1875686_POSITIVE 242 75 36 200 152 pint:PI1532|5end_uridylate_kinase_1451644..1451874_POSITIVE 241 45 5 159 122 pint:PI0253|5end_conjugative_transposon_protein,_TraD_248294..248524_POSITIVE 237 81 53 197 170 pint:PI1224|5end_sensor_histidine_kinase_and_response_regulator_1144574..1144804_POSITIVE 235 44 4 211 167 pint:PI1263|5end_probable_integrase_1184502..1184732_POSITIVE 231 77 54 226 201 pint:PI2178|5end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase,_GcpE_2117677..2117907_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 311 117 74 150 94 pint:PI0992|3end_RNA_polymerase_sigma-70_factor_917222..917372_POSITIVE 286 49 4 69 10 pint:PI0594|3end_possible_rare_lipoprotein_A_545157..545307_POSITIVE 283 75 34 149 108 pint:PI1772|3end_pullulanase_1704247..1704397_POSITIVE 270 39 1 51 8 pint:PI1196|3end_hypothetical_protein_1113630..1113780_POSITIVE 263 118 73 45 3 pint:PI1256|3end_conserved_hypothetical_protein_1178109..1178259_POSITIVE 251 67 22 85 37 pint:PI0579|3end_hypothetical_protein_524209..524359_POSITIVE 247 52 11 82 43 pint:PI0206|3end_TPR-repeat-containing_protein_199856..200006_POSITIVE 242 98 53 69 15 pint:PI1000|3end_conserved_hypothetical_protein_923672..923822_POSITIVE 231 52 11 110 75 pint:PI0329|3end_hypothetical_protein_310514..310664_POSITIVE 230 120 73 131 92 pint:PI0365|3end_hypothetical_protein_336118..336268_POSITIVE 229 76 34 92 37 pint:PI0962|3end_phosphoglycerate_mutase_892702..892852_POSITIVE 227 48 6 65 35 pint:PI0672|3end_conserved_hypothetical_protein_612230..612380_POSITIVE 226 108 61 151 94 pint:PI0873|3end_conserved_hypothetical_protein;_possible_permease_808956..809106_POSITIVE 225 116 71 146 95 pint:PI0336|3end_hypothetical_protein_314806..314956_POSITIVE 225 110 64 143 92 pint:PI0141|3end_glutamine_amidotransferase_involved_in_pyridoxine_biosynthesis_133308..133458_POSITIVE 223 79 38 120 86 pint:PI1389|3end_transcriptional_regulator,_LuxR_family_1310832..1310982_POSITIVE 223 34 3 128 98 pint:PI0513|3end_ion_transporter_472371..472521_POSITIVE 221 96 59 138 107 pint:PI0355|3end_probable__N-acetylmuramoyl-L-alanine_amidase_326698..326848_POSITIVE 220 111 74 62 21 pint:PI0832|3end_sigma-54-dependent_transcriptional_regulator_770155..770305_POSITIVE 220 66 22 138 93 pint:PI0261|3end_conjugative_transposon_protein,_TraK_254765..254915_POSITIVE