Origin IGS: taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggttttacacagtaaaagcggctgttttgcgatgcaaaatagccgcttttgcaatgccaaagcgcagttatcactttttaagagaattatctttacaagattagagatgttttcaatacttgctctgggtagtaaaaagaaaattaacatctctaatatgtaatggttatcattataaaaagaagagggaatagcgtataaagtgtgctgttccctctttcttatatattcttgtttgactttgctttaagtctttatta Mask Tandem Repeat Region ================================================ taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Find is-nt database================================================ Query_seq: PI1370:PI1371|PI1370:PI1371:alkyl hydroperoxide reductase, subunit F:hypothetical protein:->->:1292237..1292498 262 taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1370:PI1371|PI1370:PI1371:alkyl hydroperoxide reductase, subunit F:hypothetical protein:->->:1292237..1292498 262 taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1370:PI1371|PI1370:PI1371:alkyl hydroperoxide reductase, subunit F:hypothetical protein:->->:1292237..1292498 262 taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 36 End: 260 ................................... M R K R E Q H T L Y A I P S S F Y N D N H Y I L E M L I F F L L P R A S I E N I S N L V K I I L L K S D N C A L A L Q K .. Protein_Len: 43 Strand: - Start: 101 End: 229 .................................................................................................... M N S I N I K K K S G L A L I S F M E L R T F I I R K F L S L Q A K A N C F R S N Q M ................................. taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 60 End: 89 ...........................................................TTATACGCTATTCCCTCTTCTTTTTATAAT............................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 65 HP_score: -3.2 Tail_Score: -4.93296 Start: 41 End: 79 Full_Region: ACAAGAATATATAAG AAAGAGGGAACAGC ACACTTTATAC GCTATTCCCTCTTC TTTTTATAATGATAA ........................................AAAGAGGGAACAGCACACTTTATACGCTATTCCCTCTTC....................................................................................................................................................................................... TransTerm Strand: + Conf: 69 HP_score: -4.9 Tail_Score: -4.56153 Start: 44 End: 76 Full_Region: AGAATATATAAGAAA GAGGGAACAGC ACACTTTATAC GCTATTCCCTC TTCTTTTTATAATGA ...........................................GAGGGAACAGCACACTTTATACGCTATTCCCTC.......................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -12.1 Tail_Score: -4.47158 Start: 44 End: 76 Full_Region: tcattataaaaagaa gagggaatagcgtat aaa gtgtgctgttccctc tttcttatatattct ...........................................gagggaatagcgtataaagtgtgctgttccctc.......................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -16.1 Tail_Score: -4.39166 Start: 215 End: 243 Full_Region: TTTGGCATTGCAAAA GCGGCTATTTTGC ATC GCAAAACAGCCGC TTTTACTGTGTAAAA ......................................................................................................................................................................................................................GCGGCTATTTTGCATCGCAAAACAGCCGC................... Find igs database================================================ Query_seq: PI1370:PI1371|PI1370:PI1371:alkyl hydroperoxide reductase, subunit F:hypothetical protein:->->:1292237..1292498 262 taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Intra-Species Hit: Count: 13 Min: 1 Max: 262 Len: 262 Subject: pint_PI1370_PI1371|alkyl hydroperoxide reductase, subunit F:hypothetical protein|POSITIVE:POSITIVE|[1292237,1292498]|262 HSP 1 e-value: 1.0E-146 bit: 519.0 Len: 262 Query Start:1 Query End:262 Subject Strand: POSITIVE Subject Start: 1 Subject End: 262 taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta taataaagacttaaagcaaagtcaaacaagaatatataagaaagagggaacagcacactttatacgctattccctcttctttttataatgataaccattacatattagagatgttaattttctttttactacccagagcaagtattgaaaacatctctaatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta Subject: pint_PI2029_PI2030|DNA topoisomerase I:hypothetical protein|POSITIVE:POSITIVE|[1985315,1985740]|426 HSP 1 e-value: 3.0E-33 bit: 143.0 Len: 100 Query Start:163 Query End:262 Subject Strand: POSITIVE Subject Start: 143 Subject End: 242 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttgacattgcgaaagcggctcttttgcaatgcaaaacagccgcttttaccgtgcaaaaccta Subject: pint_PI0990_PI0991|GDP-mannose 4,6-dehydratase:conserved hypothetical protein|POSITIVE:POSITIVE|[915115,915448]|334 HSP 1 e-value: 3.0E-27 bit: 123.0 Len: 102 Query Start:158 Query End:259 Subject Strand: POSITIVE Subject Start: 165 Subject End: 266 .............................................................................................................................................................taatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaac... .............................................................................................................................................................taatcttgtaaagataattccgttaaaaagtgataactgcgctttggcattgcgaaagcggctcttttgcattgcaaaacctacgcttttaccgtgcaaaac... Subject: pint_PI0210_PI0211|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[203859,204206]|348 HSP 1 e-value: 5.0E-26 bit: 119.0 Len: 100 Query Start:163 Query End:262 Subject Strand: POSITIVE Subject Start: 203 Subject End: 301 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcgttgcgaaagcggct-gtttgcgatgcaaaacagccgcttttaccgtgcaaaaccta Subject: pint_PI0787_PI0788|glycosyltransferase:3-methyladenine DNA glycosylase (DNA-3-methyladenine glycosylase I)|POSITIVE:POSITIVE|[725854,726291]|438 HSP 1 e-value: 8.0E-25 bit: 115.0 Len: 102 Query Start:158 Query End:259 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 438 .............................................................................................................................................................taatcttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaac... .............................................................................................................................................................taattttgtaaagataattctcttaaaaagtgataactgcgctttgacgttgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaac... Subject: pint_PI0874_PI0875|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[810342,810747]|406 HSP 1 e-value: 3.0E-21 bit: 103.0 Len: 100 Query Start:163 Query End:262 Subject Strand: NEGATIVE Subject Start: 220 Subject End: 319 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataattcccttaaaaaaggataactgcgctttggcattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgtaaaaccta Subject: pint_PI0945_PI0946|sodium:solute symporter family protein:glutathione peroxidase|POSITIVE:POSITIVE|[877685,878112]|428 HSP 1 e-value: 7.0E-19 bit: 95.6 Len: 100 Query Start:163 Query End:262 Subject Strand: POSITIVE Subject Start: 81 Subject End: 180 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataatttcgttgaaaagtggtaattgcgctttggcattgcgaaagcggctgttttgcgatgcaaaagagccgcttttaccgtgtaaaaccta HSP 2 e-value: 3.0E-9 bit: 63.9 Len: 36 Query Start:211 Query End:246 Subject Strand: NEGATIVE Subject Start: 130 Subject End: 165 ..................................................................................................................................................................................................................aaaagcggctattttgcatcgcaaaacagccgcttt................ ..................................................................................................................................................................................................................aaaagcggctcttttgcatcgcaaaacagccgcttt................ Subject: pint_PI1360_PI1361|conserved hypothetical protein:CTP synthase (UTP-ammonia lyase)|POSITIVE:POSITIVE|[1277656,1278384]|729 HSP 1 e-value: 2.0E-16 bit: 87.7 Len: 100 Query Start:163 Query End:262 Subject Strand: POSITIVE Subject Start: 193 Subject End: 292 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataatttcgttaaaaagtgataactgcactttgacgttgcgaaagcggctgttttacgatgcaaaacagccgcttttaccgtgcaaaaccta HSP 2 e-value: 6.0E-7 bit: 56.0 Len: 36 Query Start:211 Query End:246 Subject Strand: NEGATIVE Subject Start: 242 Subject End: 277 ..................................................................................................................................................................................................................aaaagcggctattttgcatcgcaaaacagccgcttt................ ..................................................................................................................................................................................................................aaaagcggctgttttgcatcgtaaaacagccgcttt................ Subject: pint_PI1090_PI1091|tRNA uracil 5-methyltransferase:transcriptional regulator, AsnC family|POSITIVE:POSITIVE|[1015841,1016319]|479 HSP 1 e-value: 2.0E-16 bit: 87.7 Len: 100 Query Start:163 Query End:262 Subject Strand: POSITIVE Subject Start: 113 Subject End: 212 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaaaataattccgttaaaaagtgataactgccttttgacattgcaaaagcagctgttttacgatgcaaaacaaccgcttttaccgtgtaaaaccta Subject: pint_PI0831_PI0832|hypothetical protein:sigma-54-dependent transcriptional regulator|POSITIVE:POSITIVE|[768509,768973]|465 HSP 1 e-value: 2.0E-16 bit: 87.7 Len: 100 Query Start:163 Query End:262 Subject Strand: NEGATIVE Subject Start: 208 Subject End: 307 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataattccgttaaaaagtgacaactgcgctttgacgttgcgaaagcgtaggttttgcaatgcaaaacagccgcttttaccgtgcaaaaccta Subject: pint_PI0932_PI0933|conserved hypothetical protein; possible hemin receptor:hypothetical protein|POSITIVE:POSITIVE|[865425,865839]|415 HSP 1 e-value: 1.0E-11 bit: 71.9 Len: 100 Query Start:163 Query End:262 Subject Strand: NEGATIVE Subject Start: 208 Subject End: 307 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataattttattaaaaagtggcaattgcgttttgacattgcaaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaaccta Subject: pint_PI0962_PI0963|phosphoglycerate mutase:hypothetical protein|POSITIVE:POSITIVE|[892763,893337]|575 HSP 1 e-value: 6.0E-7 bit: 56.0 Len: 100 Query Start:163 Query End:262 Subject Strand: POSITIVE Subject Start: 146 Subject End: 245 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataattcgttgaaaaaatgataatttcgatttgacattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgcaaaaccta Subject: pint_PI0145_PI0146|secDF export membrane protein:transcription elongation factor|POSITIVE:POSITIVE|[139785,140477]|693 HSP 1 e-value: 6.0E-7 bit: 56.0 Len: 100 Query Start:163 Query End:262 Subject Strand: NEGATIVE Subject Start: 262 Subject End: 360 ..................................................................................................................................................................ttgtaaagataattctcttaaaaagtgataactgcgctttggcattgcaaaagcggctattttgcatcgcaaaacagccgcttttactgtgtaaaaccta ..................................................................................................................................................................ttgtaaagataattcattgaaaaac-gataatttcgatttggcattgcgaaagcgtaggttttgcaatgcaaaacagccgcttttaccgtgcaaaaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAAAGACUUAAAGCAAAGUCAAACAAGAAUAUAUAAGAAAGAGGGAACAGCACACUUUAUACGCUAUUCCCUCUUCUUUUUAUAAUGAUAACCAUUACAUAUUAGAGAUGUUAAUUUUCUUUUUACUACCCAGAGCAAGUAUUGAAAACAUCUCUAAUCUUGUAAAGAUAAUUCUCUUAAAAAGUGAUAACUGCGCUUUGGCAUUGCAAAAGCGGCUAUUUUGCAUCGCAAAACAGCCGCUUUUACUGUGUAAAACCUA .......(((((......))))).....(((((.((((((((((((((((.(((...........))).))))))))..))))))))..........(((((.((((((((((((....(((....((((..........))))..)))))))))))))))..))))).....))))).....((((((.......)))))).((((.(.((((((((((.((((((...)))))).)))))))))).).))))........ (-72.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUUUACACAGUAAAAGCGGCUGUUUUGCGAUGCAAAAUAGCCGCUUUUGCAAUGCCAAAGCGCAGUUAUCACUUUUUAAGAGAAUUAUCUUUACAAGAUUAGAGAUGUUUUCAAUACUUGCUCUGGGUAGUAAAAAGAAAAUUAACAUCUCUAAUAUGUAAUGGUUAUCAUUAUAAAAAGAAGAGGGAAUAGCGUAUAAAGUGUGCUGUUCCCUCUUUCUUAUAUAUUCUUGUUUGACUUUGCUUUAAGUCUUUAUUA .............(((((((((((((((((((...)))))))))))))))))))......((((((.(((((...(((((((.((..((.(((.(((((..(((((((((((((((..((((.((.....))))))....)))....)))))))))))).))))).))).))..)).))))))).((((((((((((((((...))))))))))))))))..................))))).))))))............ (-86.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 810 220 171 208 159 pint:PI0202|5end_DNA_helicase_191753..191983_POSITIVE 810 220 171 165 116 pint:PI0159|5end_hypothetical_protein_151273..151503_POSITIVE 810 220 171 77 28 pint:PI0157|5end_hypothetical_protein_151185..151415_POSITIVE 736 220 171 229 180 pint:PI0056|5end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_54967..55197_POSITIVE 736 220 171 77 28 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 722 220 171 154 105 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 682 220 171 228 179 pint:PI1325|5end_conserved_hypothetical_protein_1243415..1243645_POSITIVE 678 240 191 174 122 pint:PI2111|5end_conserved_hypothetical_protein_2062771..2063001_POSITIVE 659 227 181 196 150 pint:PI2003|5end_hypothetical_protein_1960823..1961053_POSITIVE 659 210 163 226 179 pint:PI0875|5end_conserved_hypothetical_protein_810608..810838_POSITIVE 655 210 163 208 161 pint:PI0007|5end_hypothetical_protein_6417..6647_POSITIVE 640 217 175 208 167 pint:PI1949|5end_aminotransferase,_class_V_1903218..1903448_POSITIVE 640 217 175 74 33 pint:PI1948|5end_hypothetical_protein_1903084..1903314_POSITIVE 640 228 181 167 120 pint:PI0771|5end_conserved_hypothetical_protein_706524..706754_POSITIVE 628 237 193 45 1 pint:PI1694|5end_hypothetical_protein_1609717..1609947_POSITIVE 614 217 171 220 174 pint:PI0552|5end_hypothetical_protein_502716..502946_POSITIVE 612 249 201 57 9 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 607 260 212 95 47 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 607 260 212 222 174 pint:PI0842|5end_5-formyltetrahydrofolate_cyclo-ligase_775533..775763_POSITIVE 607 260 212 95 47 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 810 220 171 110 61 pint:PI0200|3end_hypothetical_protein_191735..191885_POSITIVE 810 220 171 110 61 pint:PI0158|3end_hypothetical_protein_151298..151448_POSITIVE 700 220 171 150 101 pint:PI0104|3end_hypothetical_protein_99346..99496_POSITIVE 676 220 171 110 61 pint:PI0061|3end_hypothetical_protein_60690..60840_POSITIVE 673 210 163 146 99 pint:PI0099|3end_hypothetical_protein_95777..95927_POSITIVE 655 210 163 100 53 pint:PI0004|3end_hypothetical_protein_6389..6539_POSITIVE 648 220 171 70 21 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 633 260 212 144 96 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 620 220 171 114 65 pint:PI1922|3end_hypothetical_protein_1865454..1865604_POSITIVE 614 217 171 107 61 pint:PI0551|3end_hypothetical_protein_502683..502833_POSITIVE 612 249 201 139 91 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 612 220 171 120 71 pint:PI1076|3end_hypothetical_protein_999065..999215_POSITIVE 594 260 212 150 102 pint:PI2011|3end_hypothetical_protein_1970730..1970880_POSITIVE 567 217 171 121 75 pint:PI0755|3end_hypothetical_protein_686884..687034_POSITIVE 555 259 212 149 102 pint:PI0689|3end_hypothetical_protein_625877..626027_POSITIVE 554 249 201 139 91 pint:PI1340|3end_hypothetical_protein_1256872..1257022_POSITIVE 547 260 212 127 76 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 536 245 202 151 102 pint:PI1512|3end_hypothetical_protein_1429893..1430043_POSITIVE 536 259 212 50 3 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 536 259 212 50 3 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE