Origin IGS: taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaacacttttcttatagagaatagctcctttggtagaatcttattgggaagacagaaagctcgttcctttagtgtaatagaggaagatttttactttgattttgtaagaatatttcgatagaaaatcaataaaagttgttttgcgttgcgaaagcggctctttta Mask Tandem Repeat Region ================================================ taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Find is-nt database================================================ Query_seq: PI1356:PI1357|PI1356:PI1357:hypothetical protein:glycosyltransferase:->->:1273401..1273566 166 taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1356:PI1357|PI1356:PI1357:hypothetical protein:glycosyltransferase:->->:1273401..1273566 166 taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1356:PI1357|PI1356:PI1357:hypothetical protein:glycosyltransferase:->->:1273401..1273566 166 taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 48 End: 152 ............................................... R F I R V F D F Y F D E E I V S F S R A K Q R G L L I R G F S S N E M .............. taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1356:PI1357|PI1356:PI1357:hypothetical protein:glycosyltransferase:->->:1273401..1273566 166 taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Intra-Species Hit: Count: 1 Min: 1 Max: 166 Len: 166 Subject: pint_PI1356_PI1357|hypothetical protein:glycosyltransferase|POSITIVE:POSITIVE|[1273401, 1273566]|166 HSP 1 e-value: 2.0E-89 bit: 329.0 Len: 166 Query Start:1 Query End:166 Subject Strand: POSITIVE Subject Start: 1 Subject End: 166 taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta taaaagagccgctttcgcaacgcaaaacaacttttattgattttctatcgaaatattcttacaaaatcaaagtaaaaatcttcctctattacactaaaggaacgagctttctgtcttcccaataagattctaccaaaggagctattctctataagaaaagtgttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGAGCCGCUUUCGCAACGCAAAACAACUUUUAUUGAUUUUCUAUCGAAAUAUUCUUACAAAAUCAAAGUAAAAAUCUUCCUCUAUUACACUAAAGGAACGAGCUUUCUGUCUUCCCAAUAAGAUUCUACCAAAGGAGCUAUUCUCUAUAAGAAAAGUGUUUA .......((.((....))...)).(((((.(((((.((((((((.((..((.....)).)).))))))))..........(((((.((......)).)))))..(((((..((((((......))))).......)..)))))............)))))))))). (-19.41) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACACUUUUCUUAUAGAGAAUAGCUCCUUUGGUAGAAUCUUAUUGGGAAGACAGAAAGCUCGUUCCUUUAGUGUAAUAGAGGAAGAUUUUUACUUUGAUUUUGUAAGAAUAUUUCGAUAGAAAAUCAAUAAAAGUUGUUUUGCGUUGCGAAAGCGGCUCUUUUA ......(((..(....(((...((.(......).))...)))....)..)))....(((((...((((((((......))))))))((((((((..((((((((.((.((.....))..)).)))))))))))))))))))))..(((((....)))))....... (-36.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 397 50 7 154 112 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 365 50 1 206 156 pint:PI1804|5end_hypothetical_protein_1738109..1738339_POSITIVE 341 50 1 115 64 pint:PI0037|5end_hypothetical_protein_39354..39584_POSITIVE 328 28 1 227 196 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 326 50 5 229 181 pint:PI1890|5end_cell_division_protein_FtsW_1832886..1833116_POSITIVE 324 35 7 149 112 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 324 35 7 66 29 pint:PI0866|5end_hypothetical_protein_803776..804006_POSITIVE 324 35 7 217 180 pint:PI0211|5end_hypothetical_protein_204067..204297_POSITIVE 320 48 1 63 13 pint:PI2141|5end_hypothetical_protein_2087169..2087399_POSITIVE 318 50 5 114 67 pint:PI0926|5end_hypothetical_protein_856824..857054_POSITIVE 318 50 1 115 64 pint:PI0322|5end_hypothetical_protein_303508..303738_POSITIVE 313 34 1 82 43 pint:PI1528|5end_hypothetical_protein_1449685..1449915_POSITIVE 312 46 1 71 11 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 311 26 7 73 53 pint:PI0089|5end_hypothetical_protein_88162..88392_POSITIVE 307 30 1 52 21 pint:PI1057|5end_hypothetical_protein_977286..977516_POSITIVE 300 46 7 51 12 pint:PI1182|5end_hypothetical_protein_1104025..1104255_POSITIVE 297 45 1 89 43 pint:PI1786|5end_hypothetical_protein_1718124..1718354_POSITIVE 296 46 7 89 50 pint:PI0638|5end_hypothetical_protein_582218..582448_POSITIVE 295 47 1 112 63 pint:PI1436|5end_hypothetical_protein_1355961..1356191_POSITIVE 294 40 1 223 190 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 350 50 1 64 14 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 339 44 3 142 100 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 291 39 5 141 102 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 278 28 5 149 122 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE 269 30 7 26 3 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE 269 30 7 133 110 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 269 30 7 46 23 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 269 30 7 40 17 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 268 46 7 44 3 pint:PI1603|3end_hypothetical_protein_1534005..1534155_POSITIVE 262 32 1 151 122 pint:PI0200|3end_hypothetical_protein_191735..191885_POSITIVE 261 40 7 151 127 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 261 40 7 60 36 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 260 26 3 98 73 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 252 39 5 115 76 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 243 26 5 125 100 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 240 30 7 133 110 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 239 129 84 149 102 pint:PI1913|3end_conserved_hypothetical_protein_1858008..1858158_POSITIVE 237 48 6 82 28 pint:PI0472|3end_50S_ribosomal_protein_L5_438705..438855_POSITIVE 236 30 1 42 11 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 229 149 107 144 106 pint:PI1223|3end_conserved_hypothetical_protein_1144459..1144609_POSITIVE