Origin IGS: tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 actttatctttttggtttttattgtagatgcaaaaatagcgataaagtttattctttctttcaaaatttacttaaattaacagctttttactgttacttaacgcaacttggtgttgcgtatgctaacaaatataaattagaaagccccatggaggagtatttaactttgccgaacaagtaaattgttacttctttatacctcgcatcagaccaagtatccatctatccattacaacctttttctctgtgtttatctatcccgtaagcttataaaagacgggctattgagttttttaatgaggagaaaagctatataggcaaatcggaaaaagtcattaaaatttttgtattctaaattatttacacgtaaagaaatatttatttacgtgtaagaaaatatttacatgtaaataatttggcagctttttgcta Mask Tandem Repeat Region ================================================ tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Find is-nt database================================================ Query_seq: PI1314:PI1315|PI1314:PI1315:hypothetical protein:conserved hypothetical protein:->->:1231484..1231915 432 tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1314:PI1315|PI1314:PI1315:hypothetical protein:conserved hypothetical protein:->->:1231484..1231915 432 tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1314:PI1315|PI1314:PI1315:hypothetical protein:conserved hypothetical protein:->->:1231484..1231915 432 tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 51 End: 179 .................................................. M N I S L R V N N L E Y K N F N D F F R F A Y I A F L L I K K L N S P S F I S L R D R ............................................................................................................................................................................................................................................................. Protein_Len: 42 Strand: + Start: 211 End: 336 .................................................................................................................................................................................................................. M D T W S D A R Y K E V T I Y L F G K V K Y S S M G L S N L Y L L A Y A T P S C V K ................................................................................................ Protein_Len: 45 Strand: - Start: 163 End: 297 .................................................................................................................................................................. L S V P Y I F V S F F T T I S L H I S P R I R P I F F Y C N V Q E A F N F V G G H P K R M ....................................................................................................................................... Protein_Len: 44 Strand: - Start: 119 End: 250 ...................................................................................................................... I A K R R M L F S L L G D K I L K R S L Y V C L F L N Y H I S P Y K T Q H S T Y L L L M ...................................................................................................................................................................................... tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -7.9 Tail_Score: -3.61889 Start: 19 End: 54 Full_Region: cacgtaaagaaatat ttatttacgtgtaag aaaata tttacatgtaaataa tttggcagctttttg ..................ttatttacgtgtaagaaaatatttacatgtaaataa.......................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 68 HP_score: -8.0 Tail_Score: -3.06183 Start: 315 End: 331 Full_Region: ATATTTGTTAGCATA CGCAAC ACCAA GTTGCG TTAAGTAACAGTAAA ..........................................................................................................................................................................................................................................................................................................................CGCAACACCAAGTTGCG..................................................................................................... TransTerm Strand: - Conf: 66 HP_score: -8.0 Tail_Score: -2.78935 Start: 315 End: 331 Full_Region: tttactgttacttaa cgcaac ttggt gttgcg tatgctaacaaatat ..........................................................................................................................................................................................................................................................................................................................cgcaacttggtgttgcg..................................................................................................... TransTerm Strand: + Conf: 45 HP_score: -3.9 Tail_Score: -2.81966 Start: 337 End: 355 Full_Region: CCAAGTTGCGTTAAG TAACAGT AAAAA GCTGTTA ATTTAAGTAAATTTT ................................................................................................................................................................................................................................................................................................................................................TAACAGTAAAAAGCTGTTA............................................................................. Find igs database================================================ Query_seq: PI1314:PI1315|PI1314:PI1315:hypothetical protein:conserved hypothetical protein:->->:1231484..1231915 432 tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Intra-Species Hit: Count: 1 Min: 1 Max: 432 Len: 432 Subject: pint_PI1314_PI1315|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1231484,1231915]|432 HSP 1 e-value: 0.0 bit: 856.0 Len: 432 Query Start:1 Query End:432 Subject Strand: POSITIVE Subject Start: 1 Subject End: 432 tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt tagcaaaaagctgccaaattatttacatgtaaatattttcttacacgtaaataaatatttctttacgtgtaaataatttagaatacaaaaattttaatgactttttccgatttgcctatatagcttttctcctcattaaaaaactcaatagcccgtcttttataagcttacgggatagataaacacagagaaaaaggttgtaatggatagatggatacttggtctgatgcgaggtataaagaagtaacaatttacttgttcggcaaagttaaatactcctccatggggctttctaatttatatttgttagcatacgcaacaccaagttgcgttaagtaacagtaaaaagctgttaatttaagtaaattttgaaagaaagaataaactttatcgctatttttgcatctacaataaaaaccaaaaagataaagt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCAAAAAGCUGCCAAAUUAUUUACAUGUAAAUAUUUUCUUACACGUAAAUAAAUAUUUCUUUACGUGUAAAUAAUUUAGAAUACAAAAAUUUUAAUGACUUUUUCCGAUUUGCCUAUAUAGCUUUUCUCCUCAUUAAAAAACUCAAUAGCCCGUCUUUUAUAAGCUUACGGGAUAGAUAAACACAGAGAAAAAGGUUGUAAUGGAUAGAUGGAUACUUGGUCUGAUGCGAGGUAUAAAGAAGUAACAAUUUACUUGUUCGGCAAAGUUAAAUACUCCUCCAUGGGGCUUUCUAAUUUAUAUUUGUUAGCAUACGCAACACCAAGUUGCGUUAAGUAACAGUAAAAAGCUGUUAAUUUAAGUAAAUUUUGAAAGAAAGAAUAAACUUUAUCGCUAUUUUUGCAUCUACAAUAAAAACCAAAAAGAUAAAGU ((((.....))))..(((((((((((((((((((((((..(((....)))..))))))....)))))))))))))))))...........((((((..((((...(((.(((((((..(((((((((((((....................((((((((....)))...)))))...(.....)...))))))..)))))))..)).)))))))).....)))).(((((((((....((.((((((....)))))).))((..((((((.....((((.....))))(((((.(((((((............(((((((.....)))))))....(((((((.....)))))))......)))))))..)))))........))))))..))....)))))))))....................)))))) (-94.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUUAUCUUUUUGGUUUUUAUUGUAGAUGCAAAAAUAGCGAUAAAGUUUAUUCUUUCUUUCAAAAUUUACUUAAAUUAACAGCUUUUUACUGUUACUUAACGCAACUUGGUGUUGCGUAUGCUAACAAAUAUAAAUUAGAAAGCCCCAUGGAGGAGUAUUUAACUUUGCCGAACAAGUAAAUUGUUACUUCUUUAUACCUCGCAUCAGACCAAGUAUCCAUCUAUCCAUUACAACCUUUUUCUCUGUGUUUAUCUAUCCCGUAAGCUUAUAAAAGACGGGCUAUUGAGUUUUUUAAUGAGGAGAAAAGCUAUAUAGGCAAAUCGGAAAAAGUCAUUAAAAUUUUUGUAUUCUAAAUUAUUUACACGUAAAGAAAUAUUUAUUUACGUGUAAGAAAAUAUUUACAUGUAAAUAAUUUGGCAGCUUUUUGCUA ......(((((.((((...((...(((((((((((((.((((...........((((((.....((((((.......((((((.......))))))....(((((((.....))))))).............))))))))))))....(((((((((((..(((.(((((.......)))))))).)))))))))))...))))................................((((((((...(((((((....(((((...(((....))))))))......((((((((........))))))))..)))))))....))))))))........))))))))).))))...))...)).)).))))).....(((((((((((((((......)))))))))))))))..((((((....)))))) (-90.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 515 70 22 203 151 pint:PI0115|5end_hypothetical_protein_108062..108292_POSITIVE 515 70 22 189 137 pint:PI0111|5end_hypothetical_protein_108048..108278_POSITIVE 515 70 22 154 102 pint:PI0112|5end_hypothetical_protein_108013..108243_POSITIVE 488 70 22 158 106 pint:PI0130|5end_hypothetical_protein_122032..122262_POSITIVE 453 70 22 148 96 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 431 60 11 126 73 pint:PI0579|5end_hypothetical_protein_523419..523649_POSITIVE 428 80 31 161 112 pint:PI0983|5end_hypothetical_protein_909750..909980_POSITIVE 387 70 21 114 61 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 385 80 31 142 93 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 380 79 31 167 119 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 318 348 302 106 42 pint:PI1430|5end_subtilisin-like_serine_proteinase_1349598..1349828_POSITIVE 309 50 7 211 159 pint:PI0173|5end_hypothetical_protein_165305..165535_POSITIVE 308 70 21 136 83 pint:PI0648|5end_hypothetical_protein_589448..589678_POSITIVE 282 178 146 183 145 pint:PI0336|5end_hypothetical_protein_314499..314729_POSITIVE 279 52 15 231 190 pint:PI0599|5end_hypothetical_protein_547376..547606_POSITIVE 276 349 301 85 35 pint:PI1150|5end_30S_ribosomal_protein_S6_1075683..1075913_POSITIVE 272 135 108 122 93 pint:PI2162|5end_conserved_hypothetical_protein_2108796..2109026_POSITIVE 269 290 257 231 205 pint:PI1646|5end_hypothetical_protein_1561954..1562184_POSITIVE 269 33 11 73 51 pint:PI1462|5end_hypothetical_protein_1385094..1385324_POSITIVE 263 250 203 205 160 pint:PI1439|5end_hypothetical_protein_1360009..1360239_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 448 70 27 48 1 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 332 70 21 117 64 pint:PI2105|3end_hypothetical_protein_2059586..2059736_POSITIVE 330 78 33 46 1 pint:PI0808|3end_ISPg3-related_transposase_746457..746607_POSITIVE 314 245 202 110 68 pint:PI0783|3end_hypothetical_protein_723397..723547_POSITIVE 309 50 7 64 12 pint:PI0172|3end_ISPg3-related_transposase_165238..165388_POSITIVE 302 80 31 76 27 pint:PI0701|3end_hypothetical_protein_638537..638687_POSITIVE 300 79 31 130 82 pint:PI0725|3end_hypothetical_protein_658933..659083_POSITIVE 279 239 195 130 74 pint:PI1378|3end_GTP-binding_protein_lepA_1301651..1301801_POSITIVE 276 349 301 79 29 pint:PI1149|3end_30S_ribosomal_protein_S18_1075757..1075907_POSITIVE 259 72 41 50 19 pint:PI1314|3end_hypothetical_protein_1231423..1231573_POSITIVE 254 238 194 105 55 pint:PI0653|3end_hypothetical_protein_596236..596386_POSITIVE 254 216 175 71 27 pint:PI0644|3end_dnaJ_protein_586569..586719_POSITIVE 247 247 206 70 14 pint:PI1639|3end_conserved_hypothetical_protein_1558133..1558283_POSITIVE 247 181 144 47 14 pint:PI0040|3end_conserved_hypothetical_protein_(possible_integral_membrane_protein)_41872..42022_POSITIVE 246 56 18 52 10 pint:PI0797|3end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735752..735902_POSITIVE 246 168 123 90 48 pint:PI0541|3end_GTP-binding_protein,_GTP1/OBG_family_495009..495159_POSITIVE 246 331 307 136 107 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE 244 333 291 108 62 pint:PI1527|3end_immunoreactive_46_kDa_antigen_PG99_1449520..1449670_POSITIVE 243 230 187 133 69 pint:PI0246|3end_conserved_hypothetical_protein_244599..244749_POSITIVE 242 87 42 48 1 pint:PI2106|3end_hypothetical_protein_2059498..2059648_POSITIVE