Origin IGS: taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacttctctgaacggaaataactttcttaaaagtactaactcacttctttgaaatacccatttacaagcagaaaacagcaaataaaggtgtaaatatttttcacaagcgtcgctattacgtaataatcttattatcagcgtgttataaaaccta Mask Tandem Repeat Region ================================================ taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Find is-nt database================================================ Query_seq: PI1252:PI1253|PI1252:PI1253:hypothetical protein:hypothetical protein:->->:1175501..1175655 155 taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1252:PI1253|PI1252:PI1253:hypothetical protein:hypothetical protein:->->:1175501..1175655 155 taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1252:PI1253|PI1252:PI1253:hypothetical protein:hypothetical protein:->->:1175501..1175655 155 taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 17 End: 154 ................ M I I R L L R N S D A C E K Y L H L Y L L F S A C K W V F Q R S E L V L L R K L F P F R E V . Protein_Len: 45 Strand: - Start: 3 End: 137 .. T K Y C A S L L I I V Y Y R R K H F I N V G K N A T K Q K Y I P I E F F H T L V K L F T M .................. taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1252:PI1253|PI1252:PI1253:hypothetical protein:hypothetical protein:->->:1175501..1175655 155 taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Intra-Species Hit: Count: 1 Min: 1 Max: 155 Len: 155 Subject: pint_PI1252_PI1253|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1175501,1175655]|155 HSP 1 e-value: 6.0E-83 bit: 307.0 Len: 155 Query Start:1 Query End:155 Subject Strand: POSITIVE Subject Start: 1 Subject End: 155 taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta taggttttataacacgctgataataagattattacgtaatagcgacgcttgtgaaaaatatttacacctttatttgctgttttctgcttgtaaatgggtatttcaaagaagtgagttagtacttttaagaaagttatttccgttcagagaagtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUUUAUAACACGCUGAUAAUAAGAUUAUUACGUAAUAGCGACGCUUGUGAAAAAUAUUUACACCUUUAUUUGCUGUUUUCUGCUUGUAAAUGGGUAUUUCAAAGAAGUGAGUUAGUACUUUUAAGAAAGUUAUUUCCGUUCAGAGAAGUUA (((.((((.....(((..((((((....(((..(.(((.((((..(((((.(((((......(((.(((((((..((........))..))))).))))))))))...))))).)))).))))..)))....))))))..)))....)))).))) (-26.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUUCUCUGAACGGAAAUAACUUUCUUAAAAGUACUAACUCACUUCUUUGAAAUACCCAUUUACAAGCAGAAAACAGCAAAUAAAGGUGUAAAUAUUUUUCACAAGCGUCGCUAUUACGUAAUAAUCUUAUUAUCAGCGUGUUAUAAAACCUA ((((..(.((((..(((((.....(((..(.((((........)))).)..))).(((((.((((...((........))...)))))).))).....)))))....((((.......)))).............)))).).))))......... (-16.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 522 60 15 123 78 pint:PI2131|5end_hypothetical_protein_2080678..2080908_POSITIVE 509 89 42 62 9 pint:PI0670|5end_hypothetical_protein_611000..611230_POSITIVE 467 60 14 123 77 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 444 89 41 207 153 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 434 100 51 200 150 pint:PI2128|5end_hypothetical_protein_2076512..2076742_POSITIVE 420 69 26 134 89 pint:PI0200|5end_hypothetical_protein_191545..191775_POSITIVE 417 80 32 196 150 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 416 50 1 113 64 pint:PI1733|5end_hypothetical_protein_1652160..1652390_POSITIVE 413 85 41 144 98 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 401 90 47 61 20 pint:PI1814|5end_hypothetical_protein_1752953..1753183_POSITIVE 368 89 47 89 47 pint:PI0689|5end_hypothetical_protein_625624..625854_POSITIVE 362 66 26 157 117 pint:PI1694|5end_hypothetical_protein_1609717..1609947_POSITIVE 358 77 32 98 54 pint:PI0928|5end_hypothetical_protein_859851..860081_POSITIVE 352 98 51 149 99 pint:PI1057|5end_hypothetical_protein_977286..977516_POSITIVE 340 89 48 136 96 pint:PI1905|5end_hypothetical_protein_1847628..1847858_POSITIVE 322 48 1 188 141 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 322 48 1 100 53 pint:PI0053|5end_hypothetical_protein_54727..54957_POSITIVE 315 99 51 199 150 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 315 99 51 191 142 pint:PI1167|5end_hypothetical_protein_1089688..1089918_POSITIVE 314 66 32 44 10 pint:PI0759|5end_hypothetical_protein_693763..693993_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 522 60 15 76 31 pint:PI2130|3end_DNA_polymerase_I_2080711..2080861_POSITIVE 509 89 42 129 76 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 421 89 47 74 32 pint:PI1485|3end_hypothetical_protein_1405339..1405489_POSITIVE 401 90 47 114 73 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE 369 60 11 55 6 pint:PI0051|3end_hypothetical_protein_53388..53538_POSITIVE 368 89 47 135 93 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 322 48 1 122 75 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 315 99 51 59 10 pint:PI1165|3end_hypothetical_protein_1089636..1089786_POSITIVE 309 83 41 54 7 pint:PI0770|3end_hypothetical_protein_706354..706504_POSITIVE 304 89 49 85 45 pint:PI1946|3end_hypothetical_protein_1903020..1903170_POSITIVE 294 40 1 89 47 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 290 58 19 52 17 pint:PI1117|3end_glycosyl_transferase,_family_2_1042279..1042429_POSITIVE 282 88 47 62 21 pint:PI0098|3end_conserved_hypothetical_protein_(possible_glycosyl/glycerophosphate_transferase)_95532..95682_POSITIVE 276 70 36 47 12 pint:PI1424|3end_probable_peptidase_family_M48_1344044..1344194_POSITIVE 272 82 52 100 71 pint:PI2143|3end_DNA_mismatch_repair_protein_2091974..2092124_POSITIVE 260 118 71 75 30 pint:PI1965|3end_hypothetical_protein_1922906..1923056_POSITIVE 258 99 51 88 39 pint:PI2172|3end_hypothetical_protein_2113991..2114141_POSITIVE 258 99 51 61 12 pint:PI2173|3end_hypothetical_protein_2113964..2114114_POSITIVE 243 84 42 105 57 pint:PI1001|3end_hypothetical_protein_923922..924072_POSITIVE 241 67 26 71 34 pint:PI1600|3end_conserved_hypothetical_protein_1530275..1530425_POSITIVE