Origin IGS: taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagtcccctttcctcattcacttaactatacaataataaaaagccgccccagcaatccctgccaaggcggcttgacgttttaatagtcttcttgattagatgtagaactctattacaaatattttcagtttgtcgtgctttacgaaatcagctctataatacactcttttatatatcactcgtggtataccataattgtttaatttgtgcagcccttgtaagagggttgcagattgataaatggttctta Mask Tandem Repeat Region ================================================ taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Find is-nt database================================================ Query_seq: PI1229:PI1230|PI1229:PI1230:conserved hypothetical protein:probable metallo-beta-lactamase family protein:->->:1151730..1151979 250 taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1229:PI1230|PI1229:PI1230:conserved hypothetical protein:probable metallo-beta-lactamase family protein:->->:1151730..1151979 250 taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1229:PI1230|PI1229:PI1230:conserved hypothetical protein:probable metallo-beta-lactamase family protein:->->:1151730..1151979 250 taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 9 End: 152 ........ M Y Q S A T L L Q G L H K L N N Y G I P R V I Y K R V Y Y R A D F V K H D K L K I F V I E F Y I .................................................................................................. Protein_Len: 32 Strand: + Start: 131 End: 226 .................................................................................................................................. M C N R V L H L I K K T I K T S S R L G R D C W G G F L L L Y S ........................ Protein_Len: 60 Strand: - Start: 1 End: 192 D I Q L G R V L A A C I L C N H Y V V L S I Y F L T N Y L Q N R L A R C V S F I Q L L T R C R I L F V I L V D L R R P M .......................................................... taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -13.1 Tail_Score: -5.35721 Start: 180 End: 208 Full_Region: CTATTAAAACGTCAA GCCGCCTTGGCAG GGA TTGCTGGGGCGGC TTTTTATTATTGTAT ...................................................................................................................................................................................GCCGCCTTGGCAGGGATTGCTGGGGCGGC.......................................... Find igs database================================================ Query_seq: PI1229:PI1230|PI1229:PI1230:conserved hypothetical protein:probable metallo-beta-lactamase family protein:->->:1151730..1151979 250 taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Intra-Species Hit: Count: 1 Min: 1 Max: 250 Len: 250 Subject: pint_PI1229_PI1230|conserved hypothetical protein:probable metallo-beta-lactamase family protein|POSITIVE:POSITIVE|[1151730,1151979]|250 HSP 1 e-value: 1.0E-139 bit: 496.0 Len: 250 Query Start:1 Query End:250 Subject Strand: POSITIVE Subject Start: 1 Subject End: 250 taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta taagaaccatttatcaatctgcaaccctcttacaagggctgcacaaattaaacaattatggtataccacgagtgatatataaaagagtgtattatagagctgatttcgtaaagcacgacaaactgaaaatatttgtaatagagttctacatctaatcaagaagactattaaaacgtcaagccgccttggcagggattgctggggcggctttttattattgtatagttaagtgaatgaggaaaggggacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAACCAUUUAUCAAUCUGCAACCCUCUUACAAGGGCUGCACAAAUUAAACAAUUAUGGUAUACCACGAGUGAUAUAUAAAAGAGUGUAUUAUAGAGCUGAUUUCGUAAAGCACGACAAACUGAAAAUAUUUGUAAUAGAGUUCUACAUCUAAUCAAGAAGACUAUUAAAACGUCAAGCCGCCUUGGCAGGGAUUGCUGGGGCGGCUUUUUAUUAUUGUAUAGUUAAGUGAAUGAGGAAAGGGGACUA .......(((((((.....((((.((((......)))).))))..(((((.(((((.((((......(((.(((((((((......)))))))))...(((..........))).....................((((((..((((...........))))..))))))...))).(((((((((..((((...))))..))))))))).)))).))))).)))))..))))))).....((....)). (-60.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUCCCCUUUCCUCAUUCACUUAACUAUACAAUAAUAAAAAGCCGCCCCAGCAAUCCCUGCCAAGGCGGCUUGACGUUUUAAUAGUCUUCUUGAUUAGAUGUAGAACUCUAUUACAAAUAUUUUCAGUUUGUCGUGCUUUACGAAAUCAGCUCUAUAAUACACUCUUUUAUAUAUCACUCGUGGUAUACCAUAAUUGUUUAAUUUGUGCAGCCCUUGUAAGAGGGUUGCAGAUUGAUAAAUGGUUCUUA .((..((.(((..(((.((.....................((((((((...(((.....)))...))))))))...((((((.(((((.....)))))....))))))......((((((.....(((((..(((((...)))))..........................(((((((....)))))))....)))))....))))))(((((((((....))))))))).)).))).))).))..)).. (-56.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 541 210 161 205 154 pint:PI1224|5end_sensor_histidine_kinase_and_response_regulator_1144574..1144804_POSITIVE 373 208 162 82 31 pint:PI1477|5end_TonB-dependent_outer_membrane_receptor_1399734..1399964_POSITIVE 362 212 174 44 4 pint:PI0858|5end_carbamyl_phosphate_synthase,_large_subunit_790093..790323_POSITIVE 356 207 174 48 11 pint:PI0256|5end_conjugative_transposon_protein,_TraE_249044..249274_POSITIVE 355 209 163 195 136 pint:PI1891|5end_hypothetical_protein_1835196..1835426_POSITIVE 344 210 162 90 38 pint:PI1560|5end_integrase_1476962..1477192_POSITIVE 342 210 166 141 95 pint:PI2121|5end_glucose_inhibited_division_protein_A_2069889..2070119_POSITIVE 338 212 174 92 41 pint:PI1086|5end_pyruvate:ferredoxin_oxidoreductase_1006602..1006832_POSITIVE 336 208 161 47 1 pint:PI0810|5end_hypothetical_protein_746743..746973_POSITIVE 334 225 183 132 73 pint:PI0168|5end_methionyl-tRNA_synthetase_(methionine--tRNA_ligase)_161181..161411_POSITIVE 332 218 173 223 170 pint:PI1187|5end_conserved_hypothetical_protein_1106692..1106922_POSITIVE 331 222 181 96 51 pint:PI1825|5end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_1764996..1765226_POSITIVE 331 208 175 42 18 pint:PI0811|5end_protease/hemagglutinin_PrtT,_streptopain_family_746738..746968_POSITIVE 326 212 180 47 3 pint:PI1634|5end_conserved_hypothetical_protein_1553179..1553409_POSITIVE 326 212 180 47 3 pint:PI0340|5end_conserved_hypothetical_protein_316095..316325_POSITIVE 325 212 173 137 83 pint:PI0932|5end_conserved_hypothetical_protein;_possible_hemin_receptor_863854..864084_POSITIVE 324 206 173 54 15 pint:PI2018|5end_50S_ribosomal_protein_L34_1975757..1975987_POSITIVE 322 205 163 228 183 pint:PI0806|5end_heavy_metal_efflux_pump_743679..743909_POSITIVE 322 211 174 171 117 pint:PI0361|5end_hypothetical_protein_333062..333292_POSITIVE 321 208 169 62 31 pint:PI1639|5end_conserved_hypothetical_protein_1556725..1556955_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 406 220 172 86 40 pint:PI1499|3end_hypothetical_protein_1420180..1420330_POSITIVE 392 210 175 146 110 pint:PI0835|3end_conserved_hypothetical_protein;_possible_protein-export_membrane_protein_771859..772009_POSITIVE 364 209 179 57 29 pint:PI1431|3end_conserved_hypothetical_protein_1352809..1352959_POSITIVE 334 225 183 129 70 pint:PI0166|3end_hypothetical_protein_161258..161408_POSITIVE 318 215 173 144 99 pint:PI1670|3end_hypothetical_protein_1587026..1587176_POSITIVE 316 207 175 116 87 pint:PI2167|3end_hypothetical_protein_2111891..2112041_POSITIVE 314 210 172 108 66 pint:PI2074|3end_conserved_hypothetical_protein_2034375..2034525_POSITIVE 313 216 173 92 30 pint:PI0300|3end_transposase_285761..285911_POSITIVE 312 208 163 122 82 pint:PI1529|3end_cytosine/adenosine_deaminase_1450377..1450527_POSITIVE 307 217 173 46 5 pint:PI0765|3end_conserved_hypothetical_protein;_possible_surface_protein_700822..700972_POSITIVE 307 126 94 64 33 pint:PI0292|3end_ISPg-3_transposase_fragment_275904..276054_POSITIVE 306 220 179 75 22 pint:PI0910|3end_possible_sodium_hydrogen_antiporter_(Na+/H+_antiporter)_839544..839694_POSITIVE 305 209 176 133 99 pint:PI0948|3end_hypothetical_protein_881978..882128_POSITIVE 305 207 174 139 100 pint:PI0735|3end_ABC_transporter,_ATP-binding_/_permease_670591..670741_POSITIVE 305 208 180 105 74 pint:PI0644|3end_dnaJ_protein_586569..586719_POSITIVE 303 210 167 103 58 pint:PI0294|3end_conserved_hypothetical_protein_276806..276956_POSITIVE 300 221 181 144 112 pint:PI0011|3end_conserved_hypothetical_protein_13328..13478_POSITIVE 299 207 165 119 63 pint:PI0805|3end_conserved_hypothetical_protein_743807..743957_POSITIVE 299 220 179 61 27 pint:PI0033|3end_peptidylprolyl_isomerase_37101..37251_POSITIVE 297 208 174 150 113 pint:PI0595|3end_conserved_hypothetical_protein_546406..546556_POSITIVE