Origin IGS: taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcaacataacacctacatttaacaactaatagaaatgagtgaagaaactcttttttagtattctcgggaacaaagacgagatacttggcagaaaaaatttgtgcaagtttgggttgtcctttaggcaattcgtta Mask Tandem Repeat Region ================================================ taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Find is-nt database================================================ Query_seq: PI1191:PI1193|PI1191:PI1193:phosphoribosyl pyrophosphate synthetase:conserved hypothetical protein:->->:1110438..1110575 138 taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1191:PI1193|PI1191:PI1193:phosphoribosyl pyrophosphate synthetase:conserved hypothetical protein:->->:1110438..1110575 138 taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1191:PI1193|PI1191:PI1193:phosphoribosyl pyrophosphate synthetase:conserved hypothetical protein:->->:1110438..1110575 138 taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 8 End: 136 ....... M P K G Q P K L A Q I F S A K Y L V F V P E N T K K E F L H S F L L V V K C R C Y V D .. Protein_Len: 32 Strand: - Start: 2 End: 97 . V F Q R F S L G F K C L N K R G L I E D K N G L I S F F L K K M ......................................... taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1191:PI1193|PI1191:PI1193:phosphoribosyl pyrophosphate synthetase:conserved hypothetical protein:->->:1110438..1110575 138 taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Intra-Species Hit: Count: 1 Min: 1 Max: 138 Len: 138 Subject: pint_PI1191_PI1193|phosphoribosyl pyrophosphate synthetase:conserved hypothetical protein|POSITIVE:POSITIVE|[1110438,1110575]|138 HSP 1 e-value: 7.0E-73 bit: 274.0 Len: 138 Query Start:1 Query End:138 Subject Strand: POSITIVE Subject Start: 1 Subject End: 138 taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta taacgaattgcctaaaggacaacccaaacttgcacaaattttttctgccaagtatctcgtctttgttcccgagaatactaaaaaagagtttcttcactcatttctattagttgttaaatgtaggtgttatgttgatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGAAUUGCCUAAAGGACAACCCAAACUUGCACAAAUUUUUUCUGCCAAGUAUCUCGUCUUUGUUCCCGAGAAUACUAAAAAAGAGUUUCUUCACUCAUUUCUAUUAGUUGUUAAAUGUAGGUGUUAUGUUGAUUA (((((...((((((...((((((...........................(((((((((..........)))).)))))......((((......))))..........)))))).....))))))...))))).... (-25.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCAACAUAACACCUACAUUUAACAACUAAUAGAAAUGAGUGAAGAAACUCUUUUUUAGUAUUCUCGGGAACAAAGACGAGAUACUUGGCAGAAAAAAUUUGUGCAAGUUUGGGUUGUCCUUUAGGCAAUUCGUUA ..............((((......((((((..((((((.((((......)))).))))))....(((((..........))))).((((((((((.....))))).)))))...))))))....)))).......... (-26.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 305 57 14 215 165 pint:PI1881|5end_ISPg3-related_transposase_1823533..1823763_POSITIVE 289 73 32 220 179 pint:PI0764|5end_permease_697962..698192_POSITIVE 279 74 44 162 137 pint:PI0753|5end_hypothetical_protein_683391..683621_POSITIVE 266 68 22 49 3 pint:PI0785|5end_hypothetical_protein_723744..723974_POSITIVE 261 70 25 64 26 pint:PI0545|5end_sugar_kinase_496411..496641_POSITIVE 260 71 31 60 14 pint:PI0463|5end_50S_ribosomal_protein_L2_434158..434388_POSITIVE 258 65 24 90 44 pint:PI1139|5end_endopeptidase,_endothelin-converting_enzyme_1065216..1065446_POSITIVE 257 48 16 174 137 pint:PI0382|5end_conserved_hypothetical_protein_351510..351740_POSITIVE 247 48 5 161 122 pint:PI0820|5end_hypothetical_protein_759694..759924_POSITIVE 245 73 43 38 8 pint:PI0745|5end_conserved_hypothetical_protein_675174..675404_POSITIVE 241 69 23 175 130 pint:PI1388|5end_transcriptional_regulator,_LuxR_family_1309354..1309584_POSITIVE 240 57 12 71 22 pint:PI2064|5end_conserved_hypothetical_protein;_possible_type_I_restriction-modification_system,_S_subunit_2025076..2025306_POSITIVE 233 78 32 224 184 pint:PI1224|5end_sensor_histidine_kinase_and_response_regulator_1144574..1144804_POSITIVE 231 56 19 87 54 pint:PI1067|5end_thymidylate_synthase_983940..984170_POSITIVE 231 34 4 47 12 pint:PI0260|5end_conjugative_transposon_protein,_TraJ_253023..253253_POSITIVE 227 72 46 137 101 pint:PI1637|5end_hypothetical_protein_1554500..1554730_POSITIVE 226 68 43 89 58 pint:PI0601|5end_hypothetical_protein_548758..548988_POSITIVE 224 63 43 51 29 pint:PI0990|5end_GDP-mannose_4,6-dehydratase_913892..914122_POSITIVE 224 65 29 219 180 pint:PI0337|5end_hypothetical_protein_314732..314962_POSITIVE 222 70 21 169 116 pint:PI0985|5end_aspartyl-tRNA_synthetase_910174..910404_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 272 73 41 142 114 pint:PI1835|3end_L-asparaginase_I_1777276..1777426_POSITIVE 260 71 31 49 3 pint:PI0462|3end_50S_ribosomal_protein_L23_434227..434377_POSITIVE 257 48 16 138 101 pint:PI0381|3end_probable_transcriptional_regulator,_AraC_family_351554..351704_POSITIVE 247 48 5 46 7 pint:PI0818|3end_alpha-amylase_759659..759809_POSITIVE 241 68 32 48 1 pint:PI0600|3end_bifunctional_purine_biosynthesis_protein,_5-aminoimidazole-4-carboxamide_ribonucleotide(AICAR)_transformylase/IMP_cyclohydrolase_548797..548947_POSITIVE 240 57 12 71 22 pint:PI2063|3end_conserved_hypothetical_protein_2025156..2025306_POSITIVE 239 65 44 151 133 pint:PI0752|3end_conserved_hypothetical_protein_683467..683617_POSITIVE 223 48 4 72 19 pint:PI0187|3end_hypothetical_protein_178211..178361_POSITIVE 222 70 21 59 6 pint:PI0984|3end_hypothetical_protein_910144..910294_POSITIVE 221 30 2 46 12 pint:PI1004|3end_conserved_hypothetical_protein_926217..926367_POSITIVE 221 81 46 109 72 pint:PI0721|3end_hypothetical_protein_654982..655132_POSITIVE 218 69 29 48 9 pint:PI1623|3end_hypothetical_protein_1549261..1549411_POSITIVE 218 67 23 117 65 pint:PI0986|3end_conserved_hypothetical_protein_912366..912516_POSITIVE 218 69 29 78 39 pint:PI0331|3end_hypothetical_protein_312575..312725_POSITIVE 218 69 29 48 9 pint:PI0330|3end_hypothetical_protein_312545..312695_POSITIVE 215 46 9 40 5 pint:PI1686|3end_hypothetical_protein_1601898..1602048_POSITIVE 215 72 46 119 92 pint:PI0203|3end_conserved_hypothetical_protein_196064..196214_POSITIVE 214 71 43 111 81 pint:PI1746|3end_hypothetical_protein_1667606..1667756_POSITIVE 214 61 24 117 75 pint:PI0976|3end_dihydrolipoamide_dehydrogenase_(dihydrolipoyl_dehydrogenanse)_903732..903882_POSITIVE 213 74 31 131 82 pint:PI1711|3end_ABC_transporter,_ATP-binding_protein_1628429..1628579_POSITIVE