Origin IGS: tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagcttttataacacgttgataattaatgcgttatacaacaataactcttgtgaaaaatatttacacccctcttatcttctttttccctattaaataagttatttactaaaattttactcttccttgtcta Mask Tandem Repeat Region ================================================ tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Find is-nt database================================================ Query_seq: PI1165:PI1167|PI1165:PI1167:hypothetical protein:hypothetical protein:->->:1089697..1089827 131 tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1165:PI1167|PI1165:PI1167:hypothetical protein:hypothetical protein:->->:1089697..1089827 131 tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1165:PI1167|PI1165:PI1167:hypothetical protein:hypothetical protein:->->:1089697..1089827 131 tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Predict ORF larger than 30AA ================================================ tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1165:PI1167|PI1165:PI1167:hypothetical protein:hypothetical protein:->->:1089697..1089827 131 tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Intra-Species Hit: Count: 1 Min: 1 Max: 131 Len: 131 Subject: pint_PI1165_PI1167|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1089697,1089827]|131 HSP 1 e-value: 1.0E-68 bit: 260.0 Len: 131 Query Start:1 Query End:131 Subject Strand: POSITIVE Subject Start: 1 Subject End: 131 tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta tagacaaggaagagtaaaattttagtaaataacttatttaatagggaaaaagaagataagaggggtgtaaatatttttcacaagagttattgttgtataacgcattaattatcaacgtgttataaaagcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGACAAGGAAGAGUAAAAUUUUAGUAAAUAACUUAUUUAAUAGGGAAAAAGAAGAUAAGAGGGGUGUAAAUAUUUUUCACAAGAGUUAUUGUUGUAUAACGCAUUAAUUAUCAACGUGUUAUAAAAGCUA ......................((((.((((((((.(((......)))...........((((((((....))))))))....))))))))....((((((((.............))))))))...)))) (-14.52) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUUUUAUAACACGUUGAUAAUUAAUGCGUUAUACAACAAUAACUCUUGUGAAAAAUAUUUACACCCCUCUUAUCUUCUUUUUCCCUAUUAAAUAAGUUAUUUACUAAAAUUUUACUCUUCCUUGUCUA ((((((.((((((.((((((...)))))).)))))).............((((((.....))))))..............................))))))............................. (-12.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 452 94 52 101 56 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 437 96 51 219 174 pint:PI2030|5end_hypothetical_protein_1985601..1985831_POSITIVE 421 95 51 105 61 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 392 120 71 89 40 pint:PI1804|5end_hypothetical_protein_1738109..1738339_POSITIVE 387 101 61 163 123 pint:PI0949|5end_endonuclease_882264..882494_POSITIVE 376 96 51 200 155 pint:PI0324|5end_conserved_hypothetical_protein_303735..303965_POSITIVE 375 90 41 103 52 pint:PI0024|5end_hypothetical_protein_28378..28608_POSITIVE 372 84 41 231 188 pint:PI2089|5end_conserved_hypothetical_protein_2046200..2046430_POSITIVE 346 92 51 231 183 pint:PI1065|5end_hypothetical_protein_983382..983612_POSITIVE 345 83 41 107 65 pint:PI0653|5end_hypothetical_protein_596046..596276_POSITIVE 340 87 41 220 178 pint:PI1456|5end_conserved_hypothetical_protein_1378864..1379094_POSITIVE 339 103 61 142 98 pint:PI1333|5end_conserved_hypothetical_protein_1250777..1251007_POSITIVE 332 96 51 208 162 pint:PI1253|5end_hypothetical_protein_1175516..1175746_POSITIVE 328 100 51 179 130 pint:PI1272|5end_possible_transcriptional_regulator_1191702..1191932_POSITIVE 322 82 41 228 191 pint:PI1175|5end_conserved_hypothetical_protein_1097372..1097602_POSITIVE 320 87 41 185 140 pint:PI0660|5end_conserved_hypothetical_protein_603438..603668_POSITIVE 294 80 42 199 161 pint:PI1589|5end_conserved_hypothetical_protein_1517358..1517588_POSITIVE 283 107 61 198 148 pint:PI2057|5end_conserved_hypothetical_protein;_possible_metal-dependent_membrane_protease_2014939..2015169_POSITIVE 282 83 41 184 141 pint:PI0038|5end_transcriptional_regulator,_LysR_family_39578..39808_POSITIVE 278 87 48 228 189 pint:PI0286|5end_ISPg3-related_transposase_271833..272063_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 400 120 71 52 3 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 379 101 61 85 45 pint:PI1072|3end_hypothetical_protein_990503..990653_POSITIVE 350 97 51 97 45 pint:PI1064|3end_hypothetical_protein_983324..983474_POSITIVE 332 96 51 52 6 pint:PI1252|3end_hypothetical_protein_1175440..1175590_POSITIVE 320 87 41 63 18 pint:PI0659|3end_hypothetical_protein_603396..603546_POSITIVE 282 83 41 142 99 pint:PI0037|3end_hypothetical_protein_39616..39766_POSITIVE 279 87 41 46 2 pint:PI1155|3end_hypothetical_protein_1080352..1080502_POSITIVE 276 130 81 150 101 pint:PI0926|3end_hypothetical_protein_857047..857197_POSITIVE 269 97 51 97 49 pint:PI1204|3end_hypothetical_protein_1124635..1124785_POSITIVE 269 101 65 75 36 pint:PI0089|3end_hypothetical_protein_88355..88505_POSITIVE 263 83 42 42 1 pint:PI0095|3end_hypothetical_protein_91850..92000_POSITIVE 260 87 44 60 18 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 257 70 22 118 62 pint:PI0185|3end_conserved_hypothetical_protein_177821..177971_POSITIVE 253 81 41 151 110 pint:PI1804|3end_hypothetical_protein_1738290..1738440_POSITIVE 249 87 41 58 12 pint:PI1797|3end_hypothetical_protein_1731559..1731709_POSITIVE 244 130 81 128 79 pint:PI0826|3end_hypothetical_protein_764045..764195_POSITIVE 242 74 34 151 111 pint:PI1702|3end_hypothetical_protein_1620867..1621017_POSITIVE 240 101 61 133 93 pint:PI1786|3end_hypothetical_protein_1718380..1718530_POSITIVE 238 106 64 119 62 pint:PI2023|3end_hypothetical_protein_1978832..1978982_POSITIVE 233 90 42 48 1 pint:PI0638|3end_hypothetical_protein_582402..582552_POSITIVE