Origin IGS: taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaaagatatcggtgaagtagagattccttttttcgtttaaggaagattctgaaaaccaccttgtttttgtaaagataattctcttaataagtgataacttcgttttggtattgcgaaagcagctgttttgcgctgcaaaaccta Mask Tandem Repeat Region ================================================ taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Find is-nt database================================================ Query_seq: PI1141:PI1143|PI1141:PI1143:hypothetical protein:hypothetical protein:->->:1069774..1069919 146 taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1141:PI1143|PI1141:PI1143:hypothetical protein:hypothetical protein:->->:1069774..1069919 146 taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1141:PI1143|PI1141:PI1143:hypothetical protein:hypothetical protein:->->:1069774..1069919 146 taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 7 End: 105 ...... M Q R K T A A F A I P K R S Y H L L R E L S L Q K Q G G F Q N L P ......................................... Protein_Len: 30 Strand: + Start: 57 End: 146 ........................................................ M K R I I F T K T R W F S E S S L N E K R N L Y F T D I F L taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1141:PI1143|PI1141:PI1143:hypothetical protein:hypothetical protein:->->:1069774..1069919 146 taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Intra-Species Hit: Count: 1 Min: 1 Max: 146 Len: 146 Subject: pint_PI1141_PI1143|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1069774,1069919]|146 HSP 1 e-value: 1.0E-77 bit: 289.0 Len: 146 Query Start:1 Query End:146 Subject Strand: POSITIVE Subject Start: 1 Subject End: 146 taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta taggttttgcagcgcaaaacagctgctttcgcaataccaaaacgaagttatcacttattaagagaattatctttacaaaaacaaggtggttttcagaatcttccttaaacgaaaaaaggaatctctacttcaccgatatctttcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUUUGCAGCGCAAAACAGCUGCUUUCGCAAUACCAAAACGAAGUUAUCACUUAUUAAGAGAAUUAUCUUUACAAAAACAAGGUGGUUUUCAGAAUCUUCCUUAAACGAAAAAAGGAAUCUCUACUUCACCGAUAUCUUUCUA ..(((.(((((((((......)).)))...)))).))).....((((.((((.........((((((((((((.........))))))))))))......((((((.........)))))).............)))).))))... (-31.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAAGAUAUCGGUGAAGUAGAGAUUCCUUUUUUCGUUUAAGGAAGAUUCUGAAAACCACCUUGUUUUUGUAAAGAUAAUUCUCUUAAUAAGUGAUAACUUCGUUUUGGUAUUGCGAAAGCAGCUGUUUUGCGCUGCAAAACCUA ((.(((((((..((((...(((((.((((((.........))))))..))))).....)))).))))))).))..........((..(((((((....)))).)))..))..((((....))))..(((((((...)))))))... (-31.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 798 50 1 141 92 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 707 49 1 140 92 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 707 49 1 57 9 pint:PI0866|5end_hypothetical_protein_803776..804006_POSITIVE 686 50 1 97 48 pint:PI1204|5end_hypothetical_protein_1124430..1124660_POSITIVE 686 50 1 79 30 pint:PI0638|5end_hypothetical_protein_582218..582448_POSITIVE 680 50 1 82 33 pint:PI0089|5end_hypothetical_protein_88162..88392_POSITIVE 625 50 2 49 1 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 609 50 1 80 31 pint:PI1786|5end_hypothetical_protein_1718124..1718354_POSITIVE 605 33 1 231 199 pint:PI0887|5end_hypothetical_protein_817536..817766_POSITIVE 595 50 1 82 33 pint:PI0568|5end_hypothetical_protein_512950..513180_POSITIVE 585 46 1 230 185 pint:PI0024|5end_hypothetical_protein_28378..28608_POSITIVE 561 70 21 229 180 pint:PI0211|5end_hypothetical_protein_204067..204297_POSITIVE 557 52 13 43 4 pint:PI1182|5end_hypothetical_protein_1104025..1104255_POSITIVE 556 50 1 193 144 pint:PI1804|5end_hypothetical_protein_1738109..1738339_POSITIVE 554 49 4 228 183 pint:PI0196|5end_hypothetical_protein_188435..188665_POSITIVE 546 50 1 228 179 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 540 49 4 227 182 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 540 49 4 100 55 pint:PI1436|5end_hypothetical_protein_1355961..1356191_POSITIVE 536 50 4 87 41 pint:PI0659|5end_hypothetical_protein_603203..603433_POSITIVE 530 47 1 231 185 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 637 50 1 134 85 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 595 50 1 107 58 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 555 49 3 64 15 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 514 45 3 151 106 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 456 45 3 151 106 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE 442 50 1 51 2 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 358 50 3 134 84 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 340 38 4 150 111 pint:PI0158|3end_hypothetical_protein_151298..151448_POSITIVE 331 38 1 150 108 pint:PI0551|3end_hypothetical_protein_502683..502833_POSITIVE 316 29 1 150 122 pint:PI0755|3end_hypothetical_protein_686884..687034_POSITIVE 312 46 1 54 9 pint:PI0764|3end_permease_699145..699295_POSITIVE 310 94 51 45 2 pint:PI1307|3end_possible_Fe-S_oxidoreductase_1223496..1223646_POSITIVE 306 65 25 42 1 pint:PI1203|3end_conserved_hypothetical_protein_1124439..1124589_POSITIVE 305 45 5 133 94 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 305 45 5 46 7 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 305 45 5 40 1 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 304 23 1 151 129 pint:PI0637|3end_hypothetical_protein_582397..582547_POSITIVE 295 80 43 41 4 pint:PI0010|3end_conserved_hypothetical_protein_11679..11829_POSITIVE 293 45 5 46 7 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 293 45 5 74 35 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE