Origin IGS: tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagattagagtaaacaaaatcaataaataattcaaccaactttgttcgcttatatgaaacttttaaaacgcatacaggtttaaaaagggaaatataacgagaagtttaaaactaaaaacatttaaa Mask Tandem Repeat Region ================================================ tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Find is-nt database================================================ Query_seq: PI1139:PI1140|PI1139:PI1140:endopeptidase, endothelin-converting enzyme:ABC transporter, ATP-binding protein:->->:1067387..1067512 126 tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1139:PI1140|PI1139:PI1140:endopeptidase, endothelin-converting enzyme:ABC transporter, ATP-binding protein:->->:1067387..1067512 126 tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1139:PI1140|PI1139:PI1140:endopeptidase, endothelin-converting enzyme:ABC transporter, ATP-binding protein:->->:1067387..1067512 126 tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 34 End: 126 ................................. M S L F K P V C V L K V S Y K R T K L V E L F I D F V Y S N L tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1139:PI1140|PI1139:PI1140:endopeptidase, endothelin-converting enzyme:ABC transporter, ATP-binding protein:->->:1067387..1067512 126 tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Intra-Species Hit: Count: 1 Min: 1 Max: 126 Len: 126 Subject: pint_PI1139_PI1140|endopeptidase, endothelin-converting enzyme:ABC transporter, ATP-binding protein|POSITIVE:POSITIVE|[1067387,1067512]|126 HSP 1 e-value: 9.0E-66 bit: 250.0 Len: 126 Query Start:1 Query End:126 Subject Strand: POSITIVE Subject Start: 1 Subject End: 126 tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta tttaaatgtttttagttttaaacttctcgttatatttccctttttaaacctgtatgcgttttaaaagtttcatataagcgaacaaagttggttgaattatttattgattttgtttactctaatcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAAAUGUUUUUAGUUUUAAACUUCUCGUUAUAUUUCCCUUUUUAAACCUGUAUGCGUUUUAAAAGUUUCAUAUAAGCGAACAAAGUUGGUUGAAUUAUUUAUUGAUUUUGUUUACUCUAAUCUA ...........((((..............((((((....(((((.(((((.....).)))).)))))....))))))..(((((((((..((..........))..)))))))))...)))).... (-16.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAUUAGAGUAAACAAAAUCAAUAAAUAAUUCAACCAACUUUGUUCGCUUAUAUGAAACUUUUAAAACGCAUACAGGUUUAAAAAGGGAAAUAUAACGAGAAGUUUAAAACUAAAAACAUUUAAA ....((((..(((((.................(((......)))((((.((((((....(((((.((((........)))).)))))....))))))))))..)))))...))))........... (-15.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 265 57 15 216 172 pint:PI1931|5end_alpha_glycan_phosphorylase,_maltodextrin_phosphorylase_1875456..1875686_POSITIVE 248 67 26 187 138 pint:PI1333|5end_conserved_hypothetical_protein_1250777..1251007_POSITIVE 239 67 23 67 19 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 237 67 21 215 164 pint:PI0695|5end_xanthine/uracil_permease_family_protein_631413..631643_POSITIVE 231 59 11 70 25 pint:PI1483|5end_hypothetical_protein_1404310..1404540_POSITIVE 227 81 48 45 15 pint:PI1669|5end_hypothetical_protein_1581257..1581487_POSITIVE 218 89 46 217 166 pint:PI0093|5end_riboflavin_biosynthesis_protein_90489..90719_POSITIVE 211 81 49 197 171 pint:PI1580|5end_conserved_hypothetical_protein_1507833..1508063_POSITIVE 210 67 23 224 176 pint:PI1982|5end_conserved_hypothetical_protein_1938439..1938669_POSITIVE 210 67 23 224 176 pint:PI1959|5end_hypothetical_protein_1917708..1917938_POSITIVE 198 94 51 156 121 pint:PI2124|5end_excinuclease_ABC,_subunit_C_2072584..2072814_POSITIVE 198 89 49 69 29 pint:PI0196|5end_hypothetical_protein_188435..188665_POSITIVE 195 67 21 136 85 pint:PI0211|5end_hypothetical_protein_204067..204297_POSITIVE 195 85 49 231 196 pint:PI0197|5end_acetate_kinase_188602..188832_POSITIVE 194 67 23 167 120 pint:PI0594|5end_possible_rare_lipoprotein_A_544388..544618_POSITIVE 192 118 76 170 122 pint:PI0083|5end_tyrosyl-tRNA_synthetase_82824..83054_POSITIVE 191 59 13 135 92 pint:PI1251|5end_hypothetical_protein_1174740..1174970_POSITIVE 190 118 71 153 110 pint:PI1558|5end_conserved_hypothetical_protein_1473957..1474187_POSITIVE 190 88 45 188 150 pint:PI0069|5end_hypothetical_protein_67072..67302_POSITIVE 189 95 55 161 126 pint:PI0544|5end_hypoxanthine-guanine_phosphoribosyltransferase_(hypoxanthine_phosphoribosyltransferase)_495645..495875_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 67 23 121 73 pint:PI1041|3end_hypothetical_protein_960675..960825_POSITIVE 239 67 23 121 73 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 232 67 21 121 70 pint:PI1005|3end_hypothetical_protein_926481..926631_POSITIVE 227 81 48 31 1 pint:PI1668|3end_conserved_hypothetical_protein_1581323..1581473_POSITIVE 227 67 21 121 70 pint:PI0820|3end_hypothetical_protein_759980..760130_POSITIVE 227 67 21 60 9 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 227 67 26 109 60 pint:PI0638|3end_hypothetical_protein_582402..582552_POSITIVE 227 67 26 104 55 pint:PI0637|3end_hypothetical_protein_582397..582547_POSITIVE 216 68 22 110 54 pint:PI1155|3end_hypothetical_protein_1080352..1080502_POSITIVE 200 67 21 82 32 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE 200 67 26 109 60 pint:PI0081|3end_hypothetical_protein_80700..80850_POSITIVE 196 60 25 45 4 pint:PI1634|3end_conserved_hypothetical_protein_1553819..1553969_POSITIVE 194 67 23 68 21 pint:PI0593|3end_NAD-specific_glutamate_dehydrogenase_544369..544519_POSITIVE 191 59 13 96 53 pint:PI1250|3end_RNA_polymerase_ECF-type_sigma_70_factor_1174781..1174931_POSITIVE 191 82 46 150 108 pint:PI0094|3end_protoporphyrinogen_oxidase_90511..90661_POSITIVE 190 118 71 102 59 pint:PI1557|3end_conserved_hypothetical_protein_1473986..1474136_POSITIVE 190 88 45 55 17 pint:PI0068|3end_arginine_repressor_67019..67169_POSITIVE 189 76 35 86 48 pint:PI1999|3end_hypothetical_protein_1956668..1956818_POSITIVE 189 67 23 109 61 pint:PI0815|3end_hypothetical_protein_754677..754827_POSITIVE 188 63 21 113 65 pint:PI1647|3end_hypothetical_protein_1562531..1562681_POSITIVE