Origin IGS: tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattcgcaaacgcaaacatattgctttcattaaacactgacaaagaagcaatattattcctataagtcctaataagaaagcttactaacaagcacttattagggctttttattcaaaaaacatagcaccactttaacttttttatgttattagtttgtaggattcggaaactttaactatctttgcagtcaaattataattaatagaatgtgaatgtattaaggagacagtataaaccactgaatttttctccatcttttaaaaagatgtgtgtgaaacgagaaaaatagtacacgtctctatttcttttattatacataatgttatcacgcatacgagcgcgaagaactaccgcaaattacaagatacaaggtaatcccaatggatttctttttagatttttcgtgttgttttatttcaatgcaaaagagagaattaaccaaccacttacgtaatattacacccataaattcaaattaaa Mask Tandem Repeat Region ================================================ tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Find is-nt database================================================ Query_seq: PI1138:PI1139|PI1138:PI1139:hypothetical protein:endopeptidase, endothelin-converting enzyme:->->:1064874..1065355 482 tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1138:PI1139|PI1138:PI1139:hypothetical protein:endopeptidase, endothelin-converting enzyme:->->:1064874..1065355 482 tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1138:PI1139|PI1138:PI1139:hypothetical protein:endopeptidase, endothelin-converting enzyme:->->:1064874..1065355 482 tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 15 End: 152 .............. M G V I L R K W L V N S L F C I E I K Q H E K S K K K S I G I T L Y L V I C G S S S R S Y A .......................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 148 End: 279 ................................................................................................................................................... M R D N I M Y N K R N R D V Y Y F S R F T H I F L K D G E K F S G L Y C L L N T F T F Y ........................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 227 End: 337 .................................................................................................................................................................................................................................. M E K N S V V Y T V S L I H S H S I N Y N L T A K I V K V S E S Y K L I T ................................................................................................................................................. Protein_Len: 37 Strand: + Start: 370 End: 480 ................................................................................................................................................................................................................................................................................................................................................................................. M K S P N K C L L V S F L I R T Y R N N I A S L S V F N E S N M F A F A N .. Protein_Len: 35 Strand: - Start: 109 End: 213 ............................................................................................................ R T D Q L K R Y N K A S T H T I V N H I I F S I S V H V I K R T E C M ............................................................................................................................................................................................................................................................................. Protein_Len: 45 Strand: - Start: 30 End: 164 ............................. T L P Q N I R E K A N F Y F L V F F R F L F G N P N G Q I K Y N A T T R R A R I R S L M M .............................................................................................................................................................................................................................................................................................................................. Protein_Len: 41 Strand: - Start: 5 End: 124 .... * N S N I P T I N R L H N T L E R K Q M S I F C C S F D L F F D M P I V K Y R T M ...................................................................................................................................................................................................................................................................................................................................................................... tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 282 End: 311 .........................................................................................................................................................................................................................................................................................CTTTAACTATCTTTGCAGTCAAATTATAAT........................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 70 HP_score: -4.1 Tail_Score: -4.92239 Start: 92 End: 109 Full_Region: attacaagatacaag gtaatcc caat ggatttc tttttagatttttcg ...........................................................................................gtaatcccaatggatttc..................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 41 HP_score: -2.1 Tail_Score: -2.94466 Start: 240 End: 254 Full_Region: AGATGGAGAAAAATT CAGTG GTTTA TACTG TCTCCTTAATACATT ...............................................................................................................................................................................................................................................CAGTGGTTTATACTG.................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PI1138:PI1139|PI1138:PI1139:hypothetical protein:endopeptidase, endothelin-converting enzyme:->->:1064874..1065355 482 tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Intra-Species Hit: Count: 1 Min: 1 Max: 482 Len: 482 Subject: pint_PI1138_PI1139|hypothetical protein:endopeptidase, endothelin-converting enzyme|POSITIVE:POSITIVE|[1064874,1065355]|482 HSP 1 e-value: 0.0 bit: 955.0 Len: 482 Query Start:1 Query End:482 Subject Strand: POSITIVE Subject Start: 1 Subject End: 482 tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta tttaatttgaatttatgggtgtaatattacgtaagtggttggttaattctctcttttgcattgaaataaaacaacacgaaaaatctaaaaagaaatccattgggattaccttgtatcttgtaatttgcggtagttcttcgcgctcgtatgcgtgataacattatgtataataaaagaaatagagacgtgtactatttttctcgtttcacacacatctttttaaaagatggagaaaaattcagtggtttatactgtctccttaatacattcacattctattaattataatttgactgcaaagatagttaaagtttccgaatcctacaaactaataacataaaaaagttaaagtggtgctatgttttttgaataaaaagccctaataagtgcttgttagtaagctttcttattaggacttataggaataatattgcttctttgtcagtgtttaatgaaagcaatatgtttgcgtttgcgaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAAUUUGAAUUUAUGGGUGUAAUAUUACGUAAGUGGUUGGUUAAUUCUCUCUUUUGCAUUGAAAUAAAACAACACGAAAAAUCUAAAAAGAAAUCCAUUGGGAUUACCUUGUAUCUUGUAAUUUGCGGUAGUUCUUCGCGCUCGUAUGCGUGAUAACAUUAUGUAUAAUAAAAGAAAUAGAGACGUGUACUAUUUUUCUCGUUUCACACACAUCUUUUUAAAAGAUGGAGAAAAAUUCAGUGGUUUAUACUGUCUCCUUAAUACAUUCACAUUCUAUUAAUUAUAAUUUGACUGCAAAGAUAGUUAAAGUUUCCGAAUCCUACAAACUAAUAACAUAAAAAAGUUAAAGUGGUGCUAUGUUUUUUGAAUAAAAAGCCCUAAUAAGUGCUUGUUAGUAAGCUUUCUUAUUAGGACUUAUAGGAAUAAUAUUGCUUCUUUGUCAGUGUUUAAUGAAAGCAAUAUGUUUGCGUUUGCGAAUUA .(((((..((((...((((((((..(((((....)))))...................((((((.........(((((.....((((......(((((....)))))........((((......((((...(((..((((((......)))))).)))....))))......))))..))))..)))))...(((((((((..........((((((.....)))))))))))))))))))))...................)))))))).)))).)))))...(((((((((((((((((.(((......((((...........((((.((((........))))...))))...................(((.(((((((((.(((((....)))))...))))))))).)))...)))).......))))))))).))))...........((((.....)))).....))))))) (-99.22) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCGCAAACGCAAACAUAUUGCUUUCAUUAAACACUGACAAAGAAGCAAUAUUAUUCCUAUAAGUCCUAAUAAGAAAGCUUACUAACAAGCACUUAUUAGGGCUUUUUAUUCAAAAAACAUAGCACCACUUUAACUUUUUUAUGUUAUUAGUUUGUAGGAUUCGGAAACUUUAACUAUCUUUGCAGUCAAAUUAUAAUUAAUAGAAUGUGAAUGUAUUAAGGAGACAGUAUAAACCACUGAAUUUUUCUCCAUCUUUUAAAAAGAUGUGUGUGAAACGAGAAAAAUAGUACACGUCUCUAUUUCUUUUAUUAUACAUAAUGUUAUCACGCAUACGAGCGCGAAGAACUACCGCAAAUUACAAGAUACAAGGUAAUCCCAAUGGAUUUCUUUUUAGAUUUUUCGUGUUGUUUUAUUUCAAUGCAAAAGAGAGAAUUAACCAACCACUUACGUAAUAUUACACCCAUAAAUUCAAAUUAAA ..(((((((...((((.....))))...........((((.((((((...........(((((((.(((((((((((...((((......)))).)))))))))))........................(((.((((........))))..))).)))))))...(....)........))))))))))...................)))))))((((((..(((.((((.......)))).....(((((..((((((.......(((((((((...(((((..((((.........))))))))).(((((....)))))...)))))))))..((((((((((((((......(((....)))..((((.(((((....))))).)))).))).)))))))))))...............)))))))))))...........)))...))))))....................... (-90.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 478 433 82 38 pint:PI1415|5end_cation_transporting_ATPase/E1-E2_ATPase_1333939..1334169_POSITIVE 309 478 433 56 12 pint:PI1414|5end_conserved_hypothetical_protein_1333913..1334143_POSITIVE 303 460 412 187 126 pint:PI2006|5end_potassium_uptake_system_protein_1964480..1964710_POSITIVE 301 147 102 83 37 pint:PI1627|5end_hypothetical_protein_1550445..1550675_POSITIVE 297 145 109 170 122 pint:PI1646|5end_hypothetical_protein_1561954..1562184_POSITIVE 293 148 102 123 74 pint:PI0938|5end_hypothetical_protein_869345..869575_POSITIVE 291 476 436 132 82 pint:PI0837|5end_conserved_hypothetical_protein_772308..772538_POSITIVE 287 158 111 211 167 pint:PI0380|5end_conserved_hypothetical_protein_350162..350392_POSITIVE 285 154 122 40 8 pint:PI0658|5end_conserved_hypothetical_protein_602187..602417_POSITIVE 284 128 86 81 31 pint:PI1252|5end_hypothetical_protein_1175145..1175375_POSITIVE 283 167 123 75 32 pint:PI1411|5end_conserved_hypothetical_protein_1331884..1332114_POSITIVE 281 477 432 211 162 pint:PI1307|5end_possible_Fe-S_oxidoreductase_1222517..1222747_POSITIVE 274 151 111 148 101 pint:PI1848|5end_hypothetical_protein_1793799..1794029_POSITIVE 272 156 119 78 43 pint:PI1460|5end_ribonucleotide_reductase,_alpha_subunit,_N-terminal_region_1382215..1382445_POSITIVE 271 148 113 117 84 pint:PI0916|5end_hypothetical_protein_845617..845847_POSITIVE 270 159 116 177 134 pint:PI0036|5end_glutathione_peroxidase_38598..38828_POSITIVE 266 159 115 206 152 pint:PI1381|5end_conserved_hypothetical_protein_1303060..1303290_POSITIVE 264 167 124 58 18 pint:PI0364|5end_hypothetical_protein_334002..334232_POSITIVE 262 153 120 68 30 pint:PI1749|5end_conserved_hypothetical_protein_1671039..1671269_POSITIVE 261 160 114 80 38 pint:PI0967|5end_conserved_hypothetical_protein_895424..895654_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 334 170 123 63 24 pint:PI0758|3end_hypothetical_protein_690506..690656_POSITIVE 332 146 101 105 52 pint:PI2155|3end_hypothetical_protein_2101052..2101202_POSITIVE 329 150 101 151 80 pint:PI0512|3end_conserved_hypothetical_protein_471440..471590_POSITIVE 301 147 102 80 34 pint:PI1626|3end_hypothetical_protein_1550522..1550672_POSITIVE 291 476 436 117 67 pint:PI0836|3end_conserved_hypothetical_protein_772373..772523_POSITIVE 283 167 123 60 17 pint:PI1410|3end_conserved_hypothetical_protein_1331949..1332099_POSITIVE 281 477 432 66 17 pint:PI1306|3end_dipeptidyl_peptidase_IV_1222452..1222602_POSITIVE 278 154 124 54 22 pint:PI1649|3end_hypothetical_protein_1563441..1563591_POSITIVE 274 151 111 118 71 pint:PI1847|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1793849..1793999_POSITIVE 272 156 119 91 56 pint:PI1459|3end_ribonucleotide_reductase,_alpha_subunit,_C-terminal_region_1382308..1382458_POSITIVE 270 159 116 141 98 pint:PI0035|3end_NAD-dependent_protein_deacetylase,_SIR2_family_38642..38792_POSITIVE 268 477 431 119 69 pint:PI1741|3end_lipid-A-disaccharide_synthase_1663856..1664006_POSITIVE 266 160 128 50 13 pint:PI1231|3end_hypothetical_protein_1153464..1153614_POSITIVE 258 157 119 45 2 pint:PI0963|3end_hypothetical_protein_893637..893787_POSITIVE 257 150 102 133 68 pint:PI0043|3end_thiamine_phosphate_pyrophosphorylase_43156..43306_POSITIVE 256 153 111 150 110 pint:PI0628|3end_phosphoribosylglycinamide_formyltransferase_576685..576835_POSITIVE 252 156 118 88 35 pint:PI1835|3end_L-asparaginase_I_1777276..1777426_POSITIVE 251 153 111 122 77 pint:PI0972|3end_glycine_cleavage_system_H_protein_2_899399..899549_POSITIVE 250 470 429 58 21 pint:PI0984|3end_hypothetical_protein_910144..910294_POSITIVE 248 423 383 141 104 pint:PI1845|3end_hypothetical_protein_1791752..1791902_POSITIVE