Origin IGS: gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagaaggcaaagatatttatctaagtaaaaataatagcggcagatttccctattggaaatctgccgaatgaaagacgaaaaagcgtaacagaattttcctacggttggaagaaaatttctctgcggtgagaattttacacggctacggttggaaataatttttcctcaaagaaaaaatttcttccgaccgtaggaaaattcaaatactatgagagtgcacaaaaacagatactgtgaaaagtgccaaaggcgacatgaaaacaaaaaaacgacggaagggcacggagcggaaatggttcctcggaaagctcggaagttgtgtaacatttaatataaattcttaaaacaaattactatgaagatcaagcttcaaaagaaaaagaatccgcagaagcggtcagaggaaaagtattatgcaaatcctgtgacttaggacggaaaacactacgcgacattgcgcacgacattgccggacgttcgtcactgacgcgaggcgacatcgaaaac Mask Tandem Repeat Region ================================================ gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Find is-nt database================================================ Query_seq: PI1136:PI1137|PI1136:PI1137:conserved hypothetical protein:hypothetical protein:->->:1062602..1063109 508 gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1136:PI1137|PI1136:PI1137:conserved hypothetical protein:hypothetical protein:->->:1062602..1063109 508 gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1136:PI1137|PI1136:PI1137:conserved hypothetical protein:hypothetical protein:->->:1062602..1063109 508 gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 8 End: 142 ....... M S P R V S D E R P A M S C A M S R S V F R P K S Q D L H N T F P L T A S A D S F S F E A .............................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 43 Strand: + Start: 170 End: 298 ......................................................................................................................................................................... M Y I K C Y T T S E L S E E P F P L R A L P S F F C F H V A F G T F H S I C F C A L S .................................................................................................................................................................................................................. Protein_Len: 44 Strand: + Start: 174 End: 305 ............................................................................................................................................................................. M L N V T Q L P S F P R N H F R S V P F R R F F V F M S P L A L F T V S V F V H S H S I ........................................................................................................................................................................................................... Protein_Len: 53 Strand: + Start: 181 End: 339 .................................................................................................................................................................................... M L H N F R A F R G T I S A P C P S V V F L F S C R L W H F S Q Y L F L C T L I V F E F S Y G R K K F F L ......................................................................................................................................................................... Protein_Len: 53 Strand: + Start: 349 End: 507 ............................................................................................................................................................................................................................................................................................................................................................ M I S N R S R V K F S P Q R N F L P T V G K F C Y A F S S F I R Q I S N R E I C R Y Y F Y L D K Y L C L L . Protein_Len: 32 Strand: - Start: 382 End: 477 ............................................................................................................................................................................................................................................................................................................................................................................................. R L S I K K W G Y S F E T V S K R R E N P L N G I P F D A A I M ............................... Protein_Len: 40 Strand: - Start: 180 End: 299 ................................................................................................................................................................................... I N C L K R A K R P V M E A G H G E T T K K N E H R R Q C K E C Y R N K H V R M ................................................................................................................................................................................................................. Protein_Len: 36 Strand: - Start: 167 End: 274 ...................................................................................................................................................................... S N I N F T V C S G L K G L F W K R E T G K R R K K T K M D G K A S K M .......................................................................................................................................................................................................................................... Protein_Len: 57 Strand: - Start: 1 End: 171 N E I D G R T L S S R G A I D H A I D R L T K R G L D C S K C L V K G R V A E A S E K E K S A Q D E Y Y N T K L M ................................................................................................................................................................................................................................................................................................................................................. gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 448 End: 477 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTCCAATAGGGAAATCTGCCGCTATTAT............................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 43 HP_score: -3.0 Tail_Score: -2.95081 Start: 17 End: 60 Full_Region: TTTTCGATGTCGCCT CGCGTCAGTGACGAACGTC CGGCAAT GTCGTGCGCAATGTCGCG TAGTGTTTTCCGTCC ................CGCGTCAGTGACGAACGTCCGGCAATGTCGTGCGCAATGTCGCG................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 85 HP_score: -7.3 Tail_Score: -4.88407 Start: 437 End: 476 Full_Region: ACGCTTTTTCGTCTT TCATTCGGCAGATTTCC AATAG GGAAATCTGCCGCTATTA TTTTTACTTAGATAA ....................................................................................................................................................................................................................................................................................................................................................................................................................................................TCATTCGGCAGATTTCCAATAGGGAAATCTGCCGCTATTA................................ TransTerm Strand: + Conf: 88 HP_score: -9.0 Tail_Score: -4.53777 Start: 440 End: 473 Full_Region: CTTTTTCGTCTTTCA TTCGGCAGATTTCC AATAG GGAAATCTGCCGCTA TTATTTTTACTTAGA .......................................................................................................................................................................................................................................................................................................................................................................................................................................................TTCGGCAGATTTCCAATAGGGAAATCTGCCGCTA................................... TransTerm Strand: + Conf: 100 HP_score: -14.1 Tail_Score: -3.86437 Start: 441 End: 471 Full_Region: TTTTTCGTCTTTCAT TCGGCAGATTTCC AATAG GGAAATCTGCCGC TATTATTTTTACTTA ........................................................................................................................................................................................................................................................................................................................................................................................................................................................TCGGCAGATTTCCAATAGGGAAATCTGCCGC..................................... TransTerm Strand: + Conf: 100 HP_score: -17.6 Tail_Score: -2.90235 Start: 442 End: 470 Full_Region: TTTTCGTCTTTCATT CGGCAGATTTCC AATAG GGAAATCTGCCG CTATTATTTTTACTT .........................................................................................................................................................................................................................................................................................................................................................................................................................................................CGGCAGATTTCCAATAGGGAAATCTGCCG...................................... Find igs database================================================ Query_seq: PI1136:PI1137|PI1136:PI1137:conserved hypothetical protein:hypothetical protein:->->:1062602..1063109 508 gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Intra-Species Hit: Count: 1 Min: 1 Max: 508 Len: 508 Subject: pint_PI1136_PI1137|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1062602,1063109]|508 HSP 1 e-value: 0.0 bit: 965.0 Len: 508 Query Start:1 Query End:508 Subject Strand: POSITIVE Subject Start: 1 Subject End: 508 gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgnnnnnnngttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta gttttcgatgtcgcctcgcgtcagtgacgaacgtccggcaatgtcgtgcgcaatgtcgcgtagtgttttccgtcctaagtcacaggatttgcataatacttttcctctgaccgcttctgcggattctttttcttttgaagcttgatcttcatagtaatttgttttaagaatttatattaaatgttacacaacttccgagctttccgaggaaccatttccgctccgtgcccttccgtcgtttttttgttttcatgtcgcctttggcacttttcacagtatctgtttttgtgcactctcatagtatttgaattttcctacggtcggaagaaattttttctttgaggaaaaattatttccaaccgtagccgtgtaaaattctcaccgcagagaaattttcttccaaccgtaggaaaattctgttacgctttttcgtctttcattcggcagatttccaatagggaaatctgccgctattatttttacttagataaatatctttgccttctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUUCGAUGUCGCCUCGCGUCAGUGACGAACGUCCGGCAAUGUCGUGCGCAAUGUCGCGUAGUGUUUUCCGUCCUAAGUCACAGGAUUUGCAUAAUACUUUUCCUCUGACCGCUUCUGCGGAUUCUUUUUCUUUUGAAGCUUGAUCUUCAUAGUAAUUUGUUUUAAGAAUUUAUAUUAAAUGUUACACAACUUCCGAGCUUUCCGAGGAACCAUUUCCGCUCCGUGCCCUUCCGUCGUUUUUUUGUUUUCAUGUCGCCUUUGGCACUUUUCACAGUAUCUGUUUUUGUGCACUCUCAUAGUAUUUGAAUUUUCCUACGGUCGGAAGAAAUUUUUUCUUUGAGGAAAAAUUAUUUCCAACCGUAGCCGUGUAAAAUUCUCACCGCAGAGAAAUUUUCUUCCAACCGUAGGAAAAUUCUGUUACGCUUUUUCGUCUUUCAUUCGGCAGAUUUCCAAUAGGGAAAUCUGCCGCUAUUAUUUUUACUUAGAUAAAUAUCUUUGCCUUCUUA ......(((((((..((((....)))))).))))).(((((....((((((((...((.(((.(((............((((.((((...............)))).)))).......(((((.....((((((..((((((((.........(((((....(((((.........))))).)))))........))))))))..)))))).....)))))...(((((....((.(((............))).)).....))))).....))).))).))...)))))))).......(((...(((((((((((((((.((((((((.((((((.....(((((.....))))).......((.(((.........))).)).)))))).)))))))).)))))))))))))))...)))..................((((((((((((.....))))))))))))...((((((......))))))......)))))...... (-146.69) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAGGCAAAGAUAUUUAUCUAAGUAAAAAUAAUAGCGGCAGAUUUCCCUAUUGGAAAUCUGCCGAAUGAAAGACGAAAAAGCGUAACAGAAUUUUCCUACGGUUGGAAGAAAAUUUCUCUGCGGUGAGAAUUUUACACGGCUACGGUUGGAAAUAAUUUUUCCUCAAAGAAAAAAUUUCUUCCGACCGUAGGAAAAUUCAAAUACUAUGAGAGUGCACAAAAACAGAUACUGUGAAAAGUGCCAAAGGCGACAUGAAAACAAAAAAACGACGGAAGGGCACGGAGCGGAAAUGGUUCCUCGGAAAGCUCGGAAGUUGUGUAACAUUUAAUAUAAAUUCUUAAAACAAAUUACUAUGAAGAUCAAGCUUCAAAAGAAAAAGAAUCCGCAGAAGCGGUCAGAGGAAAAGUAUUAUGCAAAUCCUGUGACUUAGGACGGAAAACACUACGCGACAUUGCGCACGACAUUGCCGGACGUUCGUCACUGACGCGAGGCGACAUCGAAAAC ......(((((.........(((.((((..........((((((((((((.....))))))))))))........(((......)))....((((((((((((((((((((((((.(((((.((.((.(((((((.....((((....)))).....))))))))).)).)))))...))))))))))))))))))))))))...))))...)))((((.(((..((((...)))).....(((((...(....)..................(....)))))).((((((((....)))).......))))....((((((((.................................(((((......)))))............(((((......(((((.((((...((.....))...)))).)))))...).))))......)))))))).))).))))....))))).((.((.((((.(......).)))))).))...... (-149.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 413 238 195 165 113 pint:PI1575|5end_LemA_protein_1501143..1501373_POSITIVE 413 58 13 229 186 pint:PI1539|5end_3-phosphoshikimate_1-carboxyvinyltransferase_1459804..1460034_POSITIVE 400 40 1 90 49 pint:PI0314|5end_conserved_hypothetical_protein_295552..295782_POSITIVE 386 60 15 227 179 pint:PI2011|5end_hypothetical_protein_1970429..1970659_POSITIVE 378 59 12 219 163 pint:PI2094|5end_hypothetical_protein_2049540..2049770_POSITIVE 373 58 11 220 169 pint:PI1735|5end_conserved_hypothetical_protein_1653428..1653658_POSITIVE 372 62 24 228 184 pint:PI0576|5end_helicase,_UvrD/Rep_520226..520456_POSITIVE 369 240 195 213 156 pint:PI0263|5end_conjugative_transposon_protein,_TraM_255256..255486_POSITIVE 367 260 213 182 122 pint:PI0261|5end_conjugative_transposon_protein,_TraK_254065..254295_POSITIVE 361 72 32 200 162 pint:PI0699|5end_conserved_hypothetical_protein_636647..636877_POSITIVE 360 60 23 40 1 pint:PI0658|5end_conserved_hypothetical_protein_602187..602417_POSITIVE 353 60 13 69 29 pint:PI0705|5end_conserved_hypothetical_protein;_possible_lipoprotein_641274..641504_POSITIVE 348 49 2 72 23 pint:PI1586|5end_possible_HesA/MoeB/ThiF_family_protein_1514372..1514602_POSITIVE 347 60 11 185 143 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 347 46 2 226 169 pint:PI0155|5end_conserved_hypothetical_protein_148290..148520_POSITIVE 346 60 12 88 41 pint:PI0797|5end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735016..735246_POSITIVE 346 59 11 61 3 pint:PI0756|5end_50S_ribosomal_protein_L7/L12_687146..687376_POSITIVE 346 58 12 204 138 pint:PI0080|5end_hypothetical_protein_79047..79277_POSITIVE 344 75 36 208 168 pint:PI0251|5end_hypothetical_protein_247551..247781_POSITIVE 342 70 24 98 43 pint:PI0009|5end_NAD-dependent_epimerase/dehydratase_family_protein_9653..9883_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 403 75 32 96 52 pint:PI0365|3end_hypothetical_protein_336118..336268_POSITIVE 397 260 212 151 110 pint:PI1651|3end_hypothetical_protein_1564512..1564662_POSITIVE 382 238 191 73 30 pint:PI0683|3end_hypothetical_protein_619797..619947_POSITIVE 373 60 12 74 26 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 372 62 24 150 106 pint:PI0575|3end_hypothetical_protein_520228..520378_POSITIVE 356 50 3 101 39 pint:PI1716|3end_hypothetical_protein_1632006..1632156_POSITIVE 353 60 13 79 39 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 352 59 12 72 30 pint:PI0634|3end_conserved_hypothetical_protein_581121..581271_POSITIVE 345 60 13 57 7 pint:PI0225|3end_conserved_hypothetical_protein_222759..222909_POSITIVE 334 246 205 142 93 pint:PI0260|3end_conjugative_transposon_protein,_TraJ_254122..254272_POSITIVE 332 79 32 54 10 pint:PI1175|3end_conserved_hypothetical_protein_1098159..1098309_POSITIVE 327 59 11 65 10 pint:PI1884|3end_conserved_hypothetical_protein_1827457..1827607_POSITIVE 327 59 11 76 21 pint:PI1626|3end_hypothetical_protein_1550522..1550672_POSITIVE 323 60 11 140 84 pint:PI0736|3end_transcriptional_regulator,_TetR_family_671271..671421_POSITIVE 320 229 195 120 74 pint:PI1065|3end_hypothetical_protein_983650..983800_POSITIVE 319 59 11 83 37 pint:PI0675|3end_hypothetical_protein_613957..614107_POSITIVE 317 50 2 67 2 pint:PI1635|3end_hypothetical_protein_1554306..1554456_POSITIVE 314 49 6 62 15 pint:PI1848|3end_hypothetical_protein_1795015..1795165_POSITIVE 314 120 71 62 21 pint:PI0261|3end_conjugative_transposon_protein,_TraK_254765..254915_POSITIVE 314 74 32 142 102 pint:PI0043|3end_thiamine_phosphate_pyrophosphorylase_43156..43306_POSITIVE