Origin IGS: tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacaaagaaatacattgtaatattacttataaaaggcgttataccattggtgtaacgcctttttctaattgcgaagatagagctgaagcaaaacgtttttggagaaatagttttcttttttgttgcttgtgtcagatacta Mask Tandem Repeat Region ================================================ tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Find is-nt database================================================ Query_seq: PI1129:PI1130|PI1129:PI1130:hypothetical protein:probable glucose/galactose transporter:->->:1055664..1055805 142 tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1129:PI1130|PI1129:PI1130:hypothetical protein:probable glucose/galactose transporter:->->:1055664..1055805 142 tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1129:PI1130|PI1129:PI1130:hypothetical protein:probable glucose/galactose transporter:->->:1055664..1055805 142 tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 7 End: 141 ...... M T Q A T K K K T I S P K T F C F S S I F A I R K R R Y T N G I T P F I S N I T M Y F F V . Protein_Len: 44 Strand: - Start: 1 End: 132 L I Q C L C C F L F S N R W F R K A E A R D E C N S F P T V G I T Y R R K Y T I N C H M .......... tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 77 HP_score: -3.0 Tail_Score: -5.57396 Start: 27 End: 38 Full_Region: caaaacgtttttgga gaaa tagt tttc ttttttgttgcttgt ..........................gaaatagttttc........................................................................................................ TransTerm Strand: - Conf: 100 HP_score: -15.1 Tail_Score: -4.98255 Start: 83 End: 107 Full_Region: atattacttataaaa ggcgttatacc att ggtgtaacgcc tttttctaattgcga ..................................................................................ggcgttataccattggtgtaacgcc................................... Find igs database================================================ Query_seq: PI1129:PI1130|PI1129:PI1130:hypothetical protein:probable glucose/galactose transporter:->->:1055664..1055805 142 tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Intra-Species Hit: Count: 1 Min: 1 Max: 142 Len: 142 Subject: pint_PI1129_PI1130|hypothetical protein:probable glucose/galactose transporter|POSITIVE:POSITIVE|[1055664,1055805]|142 HSP 1 e-value: 3.0E-75 bit: 281.0 Len: 142 Query Start:1 Query End:142 Subject Strand: POSITIVE Subject Start: 1 Subject End: 142 tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta tagtatctgacacaagcaacaaaaaagaaaactatttctccaaaaacgttttgcttcagctctatcttcgcaattagaaaaaggcgttacaccaatggtataacgccttttataagtaatattacaatgtatttctttgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUAUCUGACACAAGCAACAAAAAAGAAAACUAUUUCUCCAAAAACGUUUUGCUUCAGCUCUAUCUUCGCAAUUAGAAAAAGGCGUUACACCAAUGGUAUAACGCCUUUUAUAAGUAAUAUUACAAUGUAUUUCUUUGUUA (((...((((..(((((........(((((....)))))........).))))..))))..))).....(((......(((((((((((.(((...))).)))))))))))..(((.(((((......))))).)))))).. (-25.29) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAAAGAAAUACAUUGUAAUAUUACUUAUAAAAGGCGUUAUACCAUUGGUGUAACGCCUUUUUCUAAUUGCGAAGAUAGAGCUGAAGCAAAACGUUUUUGGAGAAAUAGUUUUCUUUUUUGUUGCUUGUGUCAGAUACUA ...............(((((((.........(((((((((((((((...)))))))))))))))....)))))))...(((..((((.((((((((.....(((((((....)))))))..))))..)))).))))...))) (-36.32) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 110 61 192 137 pint:PI0815|5end_hypothetical_protein_754475..754705_POSITIVE 298 95 51 202 161 pint:PI0480|5end_preprotein_translocase,_SecY_subunit_441576..441806_POSITIVE 268 118 80 171 129 pint:PI1254|5end_probable_glucokinase_1175634..1175864_POSITIVE 268 118 80 53 11 pint:PI1253|5end_hypothetical_protein_1175516..1175746_POSITIVE 267 87 46 125 66 pint:PI0729|5end_integrase_661428..661658_POSITIVE 260 55 11 114 68 pint:PI0884|5end_hypothetical_protein_817302..817532_POSITIVE 260 55 11 92 46 pint:PI0885|5end_hypothetical_protein_817280..817510_POSITIVE 256 109 62 230 181 pint:PI1106|5end_conserved_hypothetical_protein_1030466..1030696_POSITIVE 251 130 83 124 79 pint:PI0626|5end_penicillin-binding_protein,_D-alanyl-D-alanine_carboxypeptidase_574305..574535_POSITIVE 251 82 46 88 48 pint:PI0387|5end_hypothetical_protein_354631..354861_POSITIVE 250 107 77 52 18 pint:PI1994|5end_hypothetical_protein_1949360..1949590_POSITIVE 247 98 51 53 3 pint:PI0618|5end_acyl-CoA_dehydrogenase_family_protein_567409..567639_POSITIVE 246 73 36 51 14 pint:PI0973|5end_glycine_dehydrogenase_[decarboxylating]_subunit_1_899483..899713_POSITIVE 243 88 42 209 152 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 243 88 42 85 28 pint:PI1541|5end_hypothetical_protein_1461604..1461834_POSITIVE 242 98 52 61 20 pint:PI0796|5end_competence_protein_733324..733554_POSITIVE 241 98 52 58 19 pint:PI0914|5end_ATPase,_AAA_family_843662..843892_POSITIVE 240 43 6 220 186 pint:PI0902|5end_hypothetical_protein_832291..832521_POSITIVE 240 93 52 228 189 pint:PI0167|5end_hypothetical_protein_163164..163394_POSITIVE 238 111 85 87 58 pint:PI0938|5end_hypothetical_protein_869345..869575_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 110 61 63 8 pint:PI0814|3end_conserved_hypothetical_protein_754426..754576_POSITIVE 273 77 48 116 85 pint:PI0444|3end_50S_ribosomal_protein_L7/L12_413390..413540_POSITIVE 273 77 48 85 54 pint:PI0445|3end_conserved_hypothetical_protein_413359..413509_POSITIVE 268 118 80 148 106 pint:PI1253|3end_hypothetical_protein_1175691..1175841_POSITIVE 267 87 46 125 66 pint:PI0728|3end_integrase_661508..661658_POSITIVE 251 130 83 92 47 pint:PI0625|3end_possible_membrane_transport_protein_574353..574503_POSITIVE 246 73 36 79 42 pint:PI0974|3end_hypothetical_protein_899591..899741_POSITIVE 241 98 52 49 10 pint:PI0913|3end_glycerate_dehydrogenase_843733..843883_POSITIVE 240 60 11 137 76 pint:PI1540|3end_hypothetical_protein_1461771..1461921_POSITIVE 240 43 6 56 22 pint:PI0901|3end_hypothetical_protein_832207..832357_POSITIVE 238 111 85 126 97 pint:PI0936|3end_conserved_hypothetical_protein_869464..869614_POSITIVE 234 78 31 146 106 pint:PI0944|3end_hypothetical_protein_875249..875399_POSITIVE 232 140 104 146 105 pint:PI1661|3end_hypothetical_protein_1576817..1576967_POSITIVE 226 110 61 138 92 pint:PI2071|3end_hypothetical_protein_2030308..2030458_POSITIVE 225 91 52 101 59 pint:PI2012|3end_hypothetical_protein_1971555..1971705_POSITIVE 225 100 51 61 17 pint:PI0787|3end_glycosyltransferase_725793..725943_POSITIVE 223 72 38 106 73 pint:PI1622|3end_hypothetical_protein_1547230..1547380_POSITIVE 223 107 70 130 93 pint:PI1442|3end_radical-activating_enzyme_(pyruvate-formate_lyase-activating_enzyme)_1364649..1364799_POSITIVE 223 140 104 146 105 pint:PI0368|3end_hypothetical_protein_339985..340135_POSITIVE 222 99 56 149 108 pint:PI0191|3end_O-methyltransferase_181785..181935_POSITIVE