Origin IGS: taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaggtaagaaaacaattcaagcactttctaccaatggaataaaagtttgcaacaaccttttatctatggtaagaagtgcttgaattgctatttataaatgttta Mask Tandem Repeat Region ================================================ taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Find is-nt database================================================ Query_seq: PI1091:PI1092|PI1091:PI1092:transcriptional regulator, AsnC family:conserved hypothetical protein:->->:1016815..1016919 105 taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1091:PI1092|PI1091:PI1092:transcriptional regulator, AsnC family:conserved hypothetical protein:->->:1016815..1016919 105 taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1091:PI1092|PI1091:PI1092:transcriptional regulator, AsnC family:conserved hypothetical protein:->->:1016815..1016919 105 taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Predict ORF larger than 30AA ================================================ taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 79 HP_score: -9.3 Tail_Score: -3.2505 Start: 26 End: 84 Full_Region: aagaaaacaattcaa gcactttctaccaatggaataaaagtttg caa caaccttttatctatggtaagaagtgc ttgaattgctattta .........................gcactttctaccaatggaataaaagtttgcaacaaccttttatctatggtaagaagtgc..................... Find igs database================================================ Query_seq: PI1091:PI1092|PI1091:PI1092:transcriptional regulator, AsnC family:conserved hypothetical protein:->->:1016815..1016919 105 taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Intra-Species Hit: Count: 1 Min: 1 Max: 105 Len: 105 Subject: pint_PI1091_PI1092|transcriptional regulator, AsnC family:conserved hypothetical protein|POSITIVE:POSITIVE|[1016815,1016919]|105 HSP 1 e-value: 3.0E-53 bit: 208.0 Len: 105 Query Start:1 Query End:105 Subject Strand: POSITIVE Subject Start: 1 Subject End: 105 taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta taaacatttataaatagcaattcaagcacttcttaccatagataaaaggttgttgcaaacttttattccattggtagaaagtgcttgaattgttttcttacctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACAUUUAUAAAUAGCAAUUCAAGCACUUCUUACCAUAGAUAAAAGGUUGUUGCAAACUUUUAUUCCAUUGGUAGAAAGUGCUUGAAUUGUUUUCUUACCUUA ...............((((((((((((((((..(((((..((..(((((((......)))))))..))...)))))..))))))))))))))))........... (-35.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGUAAGAAAACAAUUCAAGCACUUUCUACCAAUGGAAUAAAAGUUUGCAACAACCUUUUAUCUAUGGUAAGAAGUGCUUGAAUUGCUAUUUAUAAAUGUUUA ....(((((....((((((((((((((..(((((.((((.((((((.(((...))).)))))))))))))))..))))))))))))))...)))))......... (-36.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 254 50 5 140 88 pint:PI0281|5end_conserved_hypothetical_protein_266309..266539_POSITIVE 247 73 31 85 48 pint:PI1141|5end_hypothetical_protein_1069463..1069693_POSITIVE 232 88 46 94 43 pint:PI0732|5end_conserved_hypothetical_protein_663598..663828_POSITIVE 231 93 51 155 112 pint:PI1503|5end_30S_ribosomal_protein_S15_1423428..1423658_POSITIVE 231 61 21 213 173 pint:PI0516|5end_ferredoxin,_4Fe-4S_475864..476094_POSITIVE 226 91 53 127 86 pint:PI1511|5end_hypothetical_protein_1429580..1429810_POSITIVE 226 91 53 76 35 pint:PI1512|5end_hypothetical_protein_1429529..1429759_POSITIVE 226 57 17 108 66 pint:PI0964|5end_conserved_hypothetical_protein_893238..893468_POSITIVE 226 57 17 68 26 pint:PI0963|5end_hypothetical_protein_893198..893428_POSITIVE 226 57 16 168 119 pint:PI0831|5end_hypothetical_protein_768129..768359_POSITIVE 225 77 36 105 63 pint:PI2139|5end_cysteinyl-tRNA_synthetase_2085266..2085496_POSITIVE 223 54 23 55 27 pint:PI2177|5end_phosphoribosylaminoimidazole_carboxylase_2117127..2117357_POSITIVE 223 84 45 123 73 pint:PI0802|5end_hypothetical_protein_739544..739774_POSITIVE 222 78 35 54 21 pint:PI0555|5end_conserved_hypothetical_protein_504577..504807_POSITIVE 219 93 51 202 165 pint:PI1785|5end_conserved_hypothetical_protein_1717122..1717352_POSITIVE 219 64 30 211 184 pint:PI1492|5end_triosephosphate_isomerase_1410155..1410385_POSITIVE 218 83 47 156 119 pint:PI0691|5end_hypothetical_protein_627885..628115_POSITIVE 218 87 48 200 164 pint:PI0099|5end_hypothetical_protein_95530..95760_POSITIVE 214 76 32 57 9 pint:PI0571|5end_conserved_hypothetical_protein;_possible_permease_515161..515391_POSITIVE 213 70 22 99 48 pint:PI1115|5end_conserved_hypothetical_protein_1039244..1039474_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 308 94 53 75 33 pint:PI1091|3end_transcriptional_regulator,_AsnC_family_1016754..1016904_POSITIVE 254 50 5 125 73 pint:PI0280|3end_conserved_hypothetical_protein_266374..266524_POSITIVE 242 94 51 78 21 pint:PI0778|3end_hypothetical_protein_714885..715035_POSITIVE 232 88 46 89 38 pint:PI0731|3end_conserved_hypothetical_protein_663673..663823_POSITIVE 231 93 51 131 88 pint:PI1502|3end_hypothetical_protein_1423484..1423634_POSITIVE 226 100 68 132 103 pint:PI1236|3end_10_kDa_chaperonin,_cpn10,_GroES_1159280..1159430_POSITIVE 221 80 35 118 75 pint:PI1408|3end_glucose-1-phosphate_thymidylyltransferase_1329761..1329911_POSITIVE 220 99 51 131 77 pint:PI0496|3end_heat_shock_protein_hsp70_(chaperone_protein_dnaK)_458610..458760_POSITIVE 218 87 48 122 86 pint:PI0098|3end_conserved_hypothetical_protein_(possible_glycosyl/glycerophosphate_transferase)_95532..95682_POSITIVE 212 87 43 112 73 pint:PI0115|3end_hypothetical_protein_108669..108819_POSITIVE 210 97 51 119 70 pint:PI0615|3end_hydrolase,_haloacid_dehalogenase-like_family_565386..565536_POSITIVE 208 86 48 88 39 pint:PI0116|3end_conserved_hypothetical_protein_110769..110919_POSITIVE 205 100 51 131 84 pint:PI1372|3end_thiamine_monophosphate_kinase_1293971..1294121_POSITIVE 205 99 51 115 65 pint:PI0803|3end_DNA-damage-inducible_protein_F_741171..741321_POSITIVE 204 98 54 141 92 pint:PI1855|3end_lipopolysaccharide_biosynthesis_protein_1803168..1803318_POSITIVE 203 84 50 125 89 pint:PI1734|3end_possible_carboxyl-terminal_protease-related_protein_1653829..1653979_POSITIVE 200 99 51 107 57 pint:PI1869|3end_hypothetical_protein_1815426..1815576_POSITIVE 199 99 55 66 9 pint:PI1863|3end_phosphoribosylformylglycinamidine_synthase_1810392..1810542_POSITIVE 199 75 37 148 117 pint:PI1632|3end_hypothetical_protein_1553222..1553372_POSITIVE 199 75 37 148 117 pint:PI0339|3end_hypothetical_protein_316138..316288_POSITIVE