Origin IGS: taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agaagcaatgtaatatccactatcaagcgagcgtctcccctcctccatcaatcgtttaatattgtattgagcaaaggaaggcagcgcaaacaaagccaatatggatattacgaagcaccgaaacatttatta Mask Tandem Repeat Region ================================================ taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Find is-nt database================================================ Query_seq: PI1082:PI1083|PI1082:PI1083:polypeptide deformylase:TPR-repeat-containing protein:->->:1001983..1002114 132 taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1082:PI1083|PI1082:PI1083:polypeptide deformylase:TPR-repeat-containing protein:->->:1001983..1002114 132 taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1082:PI1083|PI1082:PI1083:polypeptide deformylase:TPR-repeat-containing protein:->->:1001983..1002114 132 taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 7 End: 132 ...... M F R C F V I S I L A L F A L P S F A Q Y N I K R L M E E G R R S L D S G Y Y I A S Protein_Len: 40 Strand: - Start: 1 End: 120 L L H K P A E Y Y G Y Q S Q K R Q R G K S L V I N F S Q H L L P S A R K I T S M ............ taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1082:PI1083|PI1082:PI1083:polypeptide deformylase:TPR-repeat-containing protein:->->:1001983..1002114 132 taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Intra-Species Hit: Count: 1 Min: 1 Max: 132 Len: 132 Subject: pint_PI1082_PI1083|polypeptide deformylase:TPR-repeat-containing protein|POSITIVE:POSITIVE|[1001983,1002114]|132 HSP 1 e-value: 3.0E-69 bit: 262.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct taataaatgtttcggtgcttcgtaatatccatattggctttgtttgcgctgccttcctttgctcaatacaatattaaacgattgatggaggaggggagacgctcgcttgatagtggatattacattgcttct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAAAUGUUUCGGUGCUUCGUAAUAUCCAUAUUGGCUUUGUUUGCGCUGCCUUCCUUUGCUCAAUACAAUAUUAAACGAUUGAUGGAGGAGGGGAGACGCUCGCUUGAUAGUGGAUAUUACAUUGCUUCU .............((.((...(((((((((((((..(....((..(((((.(((((((..(.(((((.............))))))..))))))).)).)))..)))..)).)))))))))))...)).)). (-41.32) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAAGCAAUGUAAUAUCCACUAUCAAGCGAGCGUCUCCCCUCCUCCAUCAAUCGUUUAAUAUUGUAUUGAGCAAAGGAAGGCAGCGCAAACAAAGCCAAUAUGGAUAUUACGAAGCACCGAAACAUUUAUUA ....((..(((((((((((.(((...((..(((.((.((.((((...(((((((........)).)))))....)))).)).))))).......))..))))))))))))))..))................ (-32.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 58 14 101 57 pint:PI1840|5end_conserved_hypothetical_protein_1782856..1783086_POSITIVE 337 108 61 192 132 pint:PI1851|5end_conserved_hypothetical_protein_1796847..1797077_POSITIVE 332 54 13 85 41 pint:PI1769|5end_hypothetical_protein_1696507..1696737_POSITIVE 325 129 83 223 174 pint:PI2097|5end_conserved_hypothetical_protein_2050912..2051142_POSITIVE 323 65 29 66 30 pint:PI1364|5end_dipeptidyl_peptidase_IV_1281862..1282092_POSITIVE 316 58 11 190 136 pint:PI0355|5end_probable__N-acetylmuramoyl-L-alanine_amidase_326157..326387_POSITIVE 311 117 79 221 173 pint:PI1992|5end_hypothetical_protein_1946427..1946657_POSITIVE 311 130 81 60 2 pint:PI0266|5end_DNA_primase_(bacterial_type)_257931..258161_POSITIVE 307 40 6 168 125 pint:PI2044|5end_hypothetical_protein_1996999..1997229_POSITIVE 305 53 14 214 168 pint:PI0250|5end_hypothetical_protein_246946..247176_POSITIVE 301 66 29 73 31 pint:PI0093|5end_riboflavin_biosynthesis_protein_90489..90719_POSITIVE 298 106 82 34 12 pint:PI1653|5end_hypothetical_protein_1565158..1565388_POSITIVE 292 128 81 67 5 pint:PI0641|5end_conserved_hypothetical_protein_583791..584021_POSITIVE 292 57 14 65 23 pint:PI0259|5end_conserved_protein_found_in_conjugation,_TraI_252355..252585_POSITIVE 291 56 12 64 18 pint:PI0097|5end_aspartate_aminotransferase_93235..93465_POSITIVE 290 57 15 53 4 pint:PI2090|5end_thioesterase_family_protein_2047570..2047800_POSITIVE 289 115 77 107 63 pint:PI0536|5end_hypothetical_protein_492163..492393_POSITIVE 288 55 12 220 172 pint:PI1493|5end_conserved_hypothetical_protein_1412268..1412498_POSITIVE 286 57 13 211 165 pint:PI2102|5end_DNA_topoisomerase_IV,_subunit_B_2055706..2055936_POSITIVE 286 130 99 225 185 pint:PI2083|5end_hypothetical_protein_2040324..2040554_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 116 79 68 20 pint:PI0057|3end_conserved_hypothetical_protein_56512..56662_POSITIVE 332 54 13 137 93 pint:PI1768|3end_conserved_hypothetical_protein_1696639..1696789_POSITIVE 325 129 83 115 66 pint:PI2096|3end_conserved_hypothetical_protein;_possible_phosphoesterase_2050884..2051034_POSITIVE 323 65 29 46 10 pint:PI1363|3end_hypothetical_protein_1281922..1282072_POSITIVE 311 117 79 84 36 pint:PI1991|3end_conserved_hypothetical_protein_1946370..1946520_POSITIVE 303 68 29 44 2 pint:PI1815|3end_polyphosphate-selective_porin_O_1754510..1754660_POSITIVE 298 106 82 31 9 pint:PI1652|3end_hypothetical_protein_1565235..1565385_POSITIVE 297 51 12 145 98 pint:PI0356|3end_hypothetical_protein_327288..327438_POSITIVE 296 57 14 145 103 pint:PI1493|3end_conserved_hypothetical_protein_1412818..1412968_POSITIVE 295 120 75 71 19 pint:PI1017|3end_ISPg3-related_transposase,_C-terminal_region_937661..937811_POSITIVE 291 56 12 54 8 pint:PI0096|3end_glycerol-3-phosphate_cytidylyltransferase_93305..93455_POSITIVE 290 57 15 53 4 pint:PI2089|3end_conserved_hypothetical_protein_2047650..2047800_POSITIVE 289 128 83 53 9 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 286 130 99 84 44 pint:PI2082|3end_hypothetical_protein_2040263..2040413_POSITIVE 278 50 8 150 104 pint:PI1461|3end_conserved_hypothetical_protein_1385073..1385223_POSITIVE 274 84 47 65 24 pint:PI0091|3end_hypothetical_protein_89774..89924_POSITIVE 272 52 16 61 26 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 271 68 28 148 102 pint:PI1404|3end_hypothetical_protein_1325999..1326149_POSITIVE 270 116 78 127 84 pint:PI0578|3end_methyltransferase_523253..523403_POSITIVE 268 129 81 98 46 pint:PI1185|3end_hypothetical_protein_1106557..1106707_POSITIVE