Origin IGS: taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggagaatatacctaaaaacgttcctcaacgattttaaagattaacaataaaatgccgaattgttgcaataacagttcggcatttatgtttttgaagaacttaaaaaataaggttggcgatacttattaaagctgttgattttctttttcttactcaagacgattaatgaaaatttctttggttttgtaaagataattcccttaaaaagtta Mask Tandem Repeat Region ================================================ taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Find is-nt database================================================ Query_seq: PI1076:PI1077|PI1076:PI1077:hypothetical protein:hypothetical protein:->->:999126..999338 213 taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1076:PI1077|PI1076:PI1077:hypothetical protein:hypothetical protein:->->:999126..999338 213 taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1076:PI1077|PI1076:PI1077:hypothetical protein:hypothetical protein:->->:999126..999338 213 taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 16 End: 126 ............... M I F T K P K K F S L I V L S K K K K I N S F N K Y R Q P Y F L S S S K T ....................................................................................... Protein_Len: 32 Strand: - Start: 86 End: 181 ..................................................................................... Y T D G V K N K L N K L F M F A S S N N C C N P M K N N I K L M ................................ taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 90 HP_score: -9.6 Tail_Score: -4.58283 Start: 125 End: 164 Full_Region: tttaaagattaacaa taaaatgccgaattgttg caa taacagttcggcatttatg tttttgaagaactta ............................................................................................................................taaaatgccgaattgttgcaataacagttcggcatttatg................................................. TransTerm Strand: + Conf: 100 HP_score: -10.4 Tail_Score: -4.93661 Start: 131 End: 159 Full_Region: CTTCAAAAACATAAA TGCCGAACTGTT ATTGC AACAATTCGGCA TTTTATTGTTAATCT ..................................................................................................................................TGCCGAACTGTTATTGCAACAATTCGGCA...................................................... TransTerm Strand: - Conf: 100 HP_score: -13.3 Tail_Score: -4.55887 Start: 131 End: 159 Full_Region: agattaacaataaaa tgccgaattgttg caa taacagttcggca tttatgtttttgaag ..................................................................................................................................tgccgaattgttgcaataacagttcggca...................................................... Find igs database================================================ Query_seq: PI1076:PI1077|PI1076:PI1077:hypothetical protein:hypothetical protein:->->:999126..999338 213 taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Intra-Species Hit: Count: 1 Min: 1 Max: 213 Len: 213 Subject: pint_PI1076_PI1077|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[999126,999338]|213 HSP 1 e-value: 1.0E-117 bit: 422.0 Len: 213 Query Start:1 Query End:213 Subject Strand: POSITIVE Subject Start: 1 Subject End: 213 taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta taactttttaagggaattatctttacaaaaccaaagaaattttcattaatcgtcttgagtaagaaaaagaaaatcaacagctttaataagtatcgccaaccttattttttaagttcttcaaaaacataaatgccgaactgttattgcaacaattcggcattttattgttaatctttaaaatcgttgaggaacgtttttaggtatattctccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUUUUUAAGGGAAUUAUCUUUACAAAACCAAAGAAAUUUUCAUUAAUCGUCUUGAGUAAGAAAAAGAAAAUCAACAGCUUUAAUAAGUAUCGCCAACCUUAUUUUUUAAGUUCUUCAAAAACAUAAAUGCCGAACUGUUAUUGCAACAAUUCGGCAUUUUAUUGUUAAUCUUUAAAAUCGUUGAGGAACGUUUUUAGGUAUAUUCUCCUA ..((((.((((((......(((((........)))))..(((((.(((.((.....)).)))))))).............)))))).)))).....................(((((((((.((((.((((((((((.((((.....)))).))))))))))...))))..............))))))))).....((((........)))) (-40.56) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGAGAAUAUACCUAAAAACGUUCCUCAACGAUUUUAAAGAUUAACAAUAAAAUGCCGAAUUGUUGCAAUAACAGUUCGGCAUUUAUGUUUUUGAAGAACUUAAAAAAUAAGGUUGGCGAUACUUAUUAAAGCUGUUGAUUUUCUUUUUCUUACUCAAGACGAUUAAUGAAAAUUUCUUUGGUUUUGUAAAGAUAAUUCCCUUAAAAAGUUA .(((.((((.................((((((.(((((((((((..((((.((((((((((((((((...))))))))))))))))...(((((((.....))))))).....))))..))).))..)))))).)))))).........((((...((((((.(..(((....)))....).))))))..))))..))))))).......... (-45.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 369 44 3 49 8 pint:PI1072|5end_hypothetical_protein_990220..990450_POSITIVE 367 36 3 78 46 pint:PI1041|5end_hypothetical_protein_960443..960673_POSITIVE 347 36 3 63 31 pint:PI1540|5end_hypothetical_protein_1461362..1461592_POSITIVE 320 60 13 49 8 pint:PI0069|5end_hypothetical_protein_67072..67302_POSITIVE 315 36 3 85 52 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 306 60 11 142 93 pint:PI0089|5end_hypothetical_protein_88162..88392_POSITIVE 298 45 3 208 166 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 292 44 3 46 5 pint:PI0902|5end_hypothetical_protein_832291..832521_POSITIVE 281 45 3 142 100 pint:PI1204|5end_hypothetical_protein_1124430..1124660_POSITIVE 280 29 3 33 7 pint:PI1308|5end_hypothetical_protein_1223419..1223649_POSITIVE 265 58 13 99 54 pint:PI1182|5end_hypothetical_protein_1104025..1104255_POSITIVE 263 60 11 149 94 pint:PI0568|5end_hypothetical_protein_512950..513180_POSITIVE 256 119 91 55 31 pint:PI1409|5end_conserved_hypothetical_protein_1330849..1331079_POSITIVE 254 172 131 166 128 pint:PI2121|5end_glucose_inhibited_division_protein_A_2069889..2070119_POSITIVE 254 69 21 64 16 pint:PI0826|5end_hypothetical_protein_763738..763968_POSITIVE 253 89 46 64 18 pint:PI0825|5end_hypothetical_protein_763718..763948_POSITIVE 249 45 3 59 17 pint:PI0884|5end_hypothetical_protein_817302..817532_POSITIVE 249 44 5 185 146 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 248 48 6 47 1 pint:PI1005|5end_hypothetical_protein_926201..926431_POSITIVE 245 197 151 94 42 pint:PI2135|5end_conserved_hypothetical_protein_2082754..2082984_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 299 36 4 33 1 pint:PI1063|3end_excinuclease_ABC,_subunit_B_983099..983249_POSITIVE 281 45 3 71 29 pint:PI1203|3end_conserved_hypothetical_protein_1124439..1124589_POSITIVE 280 29 3 30 4 pint:PI1307|3end_possible_Fe-S_oxidoreductase_1223496..1223646_POSITIVE 278 169 125 49 3 pint:PI1970|3end_hypothetical_protein_1927882..1928032_POSITIVE 271 168 123 99 50 pint:PI1380|3end_conserved_hypothetical_protein_1303242..1303392_POSITIVE 267 31 3 42 14 pint:PI0010|3end_conserved_hypothetical_protein_11679..11829_POSITIVE 256 38 5 143 113 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 255 47 3 53 5 pint:PI1379|3end_hypothetical_protein_1302292..1302442_POSITIVE 249 169 125 135 96 pint:PI0365|3end_hypothetical_protein_336118..336268_POSITIVE 245 197 151 100 48 pint:PI2136|3end_hypothetical_protein_2082840..2082990_POSITIVE 245 197 151 70 18 pint:PI2134|3end_hypothetical_protein_2082810..2082960_POSITIVE 236 169 121 71 18 pint:PI2108|3end_cell_surface_protein;_possible_cell_surface_antigen_BspA_2060625..2060775_POSITIVE 235 59 14 50 1 pint:PI1881|3end_ISPg3-related_transposase_1824530..1824680_POSITIVE 234 180 132 85 36 pint:PI1847|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1793849..1793999_POSITIVE 225 174 134 86 40 pint:PI0743|3end_hypothetical_protein_673026..673176_POSITIVE 221 159 130 62 27 pint:PI0092|3end_regulatory_protein_RecX_89651..89801_POSITIVE 217 159 114 145 95 pint:PI0160|3end_conserved_hypothetical_protein_153268..153418_POSITIVE 214 158 111 53 6 pint:PI2148|3end_ribonuclease_III_2095541..2095691_POSITIVE 214 155 112 117 75 pint:PI0138|3end_hypothetical_protein_131365..131515_POSITIVE 212 170 125 140 95 pint:PI0565|3end_hypothetical_protein_511657..511807_POSITIVE