Origin IGS: tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaagcgaaaacaatagaagttttcaatactaaaaaataatagaacttgaatatactataaacagaaagattttctcgtattttataaacagaaaacaaacgatacaggtgtaaatatttttcacaagaatatcta Mask Tandem Repeat Region ================================================ tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Find is-nt database================================================ Query_seq: PI1064:PI1065|PI1064:PI1065:hypothetical protein:hypothetical protein:->->:983385..983521 137 tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1064:PI1065|PI1064:PI1065:hypothetical protein:hypothetical protein:->->:983385..983521 137 tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1064:PI1065|PI1064:PI1065:hypothetical protein:hypothetical protein:->->:983385..983521 137 tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 6 End: 104 ..... M L V K N I Y T C I V C F L F I K Y E K I F L F I V Y S S S I I F ................................. Protein_Len: 36 Strand: + Start: 29 End: 136 ............................ M Y R L F S V Y K I R E N L S V Y S I F K F Y Y F L V L K T S I V F A S . tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1064:PI1065|PI1064:PI1065:hypothetical protein:hypothetical protein:->->:983385..983521 137 tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: pint_PI1064_PI1065|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[983385,983521]|137 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta tagatattcttgtgaaaaatatttacacctgtatcgtttgttttctgtttataaaatacgagaaaatctttctgtttatagtatattcaagttctattattttttagtattgaaaacttctattgttttcgcttcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAUAUUCUUGUGAAAAAUAUUUACACCUGUAUCGUUUGUUUUCUGUUUAUAAAAUACGAGAAAAUCUUUCUGUUUAUAGUAUAUUCAAGUUCUAUUAUUUUUUAGUAUUGAAAACUUCUAUUGUUUUCGCUUCUA ..(((((...((((((.....))))))...))))).((((........(((((((....(((.....)))....)))))))......)))).............(((...(((((((.......)))))))...))) (-17.44) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAGCGAAAACAAUAGAAGUUUUCAAUACUAAAAAAUAAUAGAACUUGAAUAUACUAUAAACAGAAAGAUUUUCUCGUAUUUUAUAAACAGAAAACAAACGAUACAGGUGUAAAUAUUUUUCACAAGAAUAUCUA ((((...((((((.......))))))....................((((..((((..(((...((((.....))))..)))..))))....(((((......(((....)))......))))).))))....)))) (-14.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 459 50 1 207 158 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 409 60 11 162 113 pint:PI0200|5end_hypothetical_protein_191545..191775_POSITIVE 394 50 1 89 40 pint:PI0689|5end_hypothetical_protein_625624..625854_POSITIVE 374 45 9 148 111 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 372 58 11 160 113 pint:PI0004|5end_hypothetical_protein_6199..6429_POSITIVE 363 50 2 136 89 pint:PI1905|5end_hypothetical_protein_1847628..1847858_POSITIVE 354 42 1 61 13 pint:PI1281|5end_hypothetical_protein_1196645..1196875_POSITIVE 353 60 11 70 23 pint:PI0670|5end_hypothetical_protein_611000..611230_POSITIVE 325 46 6 180 144 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 325 46 6 172 136 pint:PI1167|5end_hypothetical_protein_1089688..1089918_POSITIVE 312 50 1 188 139 pint:PI2128|5end_hypothetical_protein_2076512..2076742_POSITIVE 298 60 11 122 72 pint:PI0928|5end_hypothetical_protein_859851..860081_POSITIVE 290 42 1 55 18 pint:PI0759|5end_hypothetical_protein_693763..693993_POSITIVE 288 45 1 158 114 pint:PI0755|5end_hypothetical_protein_686691..686921_POSITIVE 287 58 11 147 102 pint:PI1057|5end_hypothetical_protein_977286..977516_POSITIVE 285 50 1 60 13 pint:PI1814|5end_hypothetical_protein_1752953..1753183_POSITIVE 283 60 11 163 113 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 280 60 13 79 31 pint:PI0187|5end_hypothetical_protein_177856..178086_POSITIVE 276 46 10 225 189 pint:PI1948|5end_hypothetical_protein_1903084..1903314_POSITIVE 274 32 1 163 131 pint:PI1601|5end_hypothetical_protein_1530282..1530512_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 394 50 1 135 86 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 377 50 2 87 36 pint:PI0051|3end_hypothetical_protein_53388..53538_POSITIVE 353 60 11 137 90 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 325 46 6 40 4 pint:PI1165|3end_hypothetical_protein_1089636..1089786_POSITIVE 299 58 11 82 35 pint:PI1485|3end_hypothetical_protein_1405339..1405489_POSITIVE 298 45 9 61 24 pint:PI1424|3end_probable_peptidase_family_M48_1344044..1344194_POSITIVE 285 50 1 113 66 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE 280 48 2 117 67 pint:PI1391|3end_hypothetical_protein_1311694..1311844_POSITIVE 280 60 13 146 98 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 276 46 10 81 45 pint:PI1946|3end_hypothetical_protein_1903020..1903170_POSITIVE 274 32 1 76 44 pint:PI1600|3end_conserved_hypothetical_protein_1530275..1530425_POSITIVE 255 49 9 62 22 pint:PI0098|3end_conserved_hypothetical_protein_(possible_glycosyl/glycerophosphate_transferase)_95532..95682_POSITIVE 252 32 1 86 56 pint:PI2130|3end_DNA_polymerase_I_2080711..2080861_POSITIVE 252 45 19 47 18 pint:PI0358|3end_hypothetical_protein_328105..328255_POSITIVE 250 50 1 77 28 pint:PI2172|3end_hypothetical_protein_2113991..2114141_POSITIVE 250 50 1 50 1 pint:PI2173|3end_hypothetical_protein_2113964..2114114_POSITIVE 249 56 14 44 1 pint:PI0687|3end_hypothetical_protein_625652..625802_POSITIVE 247 50 1 130 82 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 245 41 3 62 14 pint:PI0770|3end_hypothetical_protein_706354..706504_POSITIVE 242 54 11 81 36 pint:PI0412|3end_primosomal_protein_N',_replication_factor_Y_382152..382302_POSITIVE