Origin IGS: tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatttatagtacctctacaaataaaaacaagagaattaaggagttttagcatgctgcatcaattgagacacaacttgtttcattgcacaagcaacaatggcttaactccttaatttcattatataacgctttaacttccctctata Mask Tandem Repeat Region ================================================ tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Find is-nt database================================================ Query_seq: PI1025:PI1026|PI1025:PI1026:tRNA modification GTPase:uridine phosphorylase:->->:942293..942439 147 tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1025:PI1026|PI1025:PI1026:tRNA modification GTPase:uridine phosphorylase:->->:942293..942439 147 tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1025:PI1026|PI1025:PI1026:tRNA modification GTPase:uridine phosphorylase:->->:942293..942439 147 tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 37 End: 141 .................................... P T L G N N S T C H F L N H R L Q H L M S F S R L E R T K I Q L P V M ...... tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1025:PI1026|PI1025:PI1026:tRNA modification GTPase:uridine phosphorylase:->->:942293..942439 147 tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Intra-Species Hit: Count: 1 Min: 1 Max: 147 Len: 147 Subject: pint_PI1025_PI1026|tRNA modification GTPase:uridine phosphorylase|POSITIVE:POSITIVE|[942293,942439]|147 HSP 1 e-value: 3.0E-78 bit: 291.0 Len: 147 Query Start:1 Query End:147 Subject Strand: POSITIVE Subject Start: 1 Subject End: 147 tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta tatagagggaagttaaagcgttatataatgaaattaaggagttaagccattgttgcttgtgcaatgaaacaagttgtgtctcaattgatgcagcatgctaaaactccttaattctcttgtttttatttgtagaggtactataaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAGAGGGAAGUUAAAGCGUUAUAUAAUGAAAUUAAGGAGUUAAGCCAUUGUUGCUUGUGCAAUGAAACAAGUUGUGUCUCAAUUGAUGCAGCAUGCUAAAACUCCUUAAUUCUCUUGUUUUUAUUUGUAGAGGUACUAUAAAUUA ....((((((........((((....))))..(((((((((((.(((..(..((((....))))..).....((((((((......))))))))..)))..)))))))))))))))))......((((((((.....)))))))).. (-39.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUAUAGUACCUCUACAAAUAAAAACAAGAGAAUUAAGGAGUUUUAGCAUGCUGCAUCAAUUGAGACACAACUUGUUUCAUUGCACAAGCAACAAUGGCUUAACUCCUUAAUUUCAUUAUAUAACGCUUUAACUUCCCUCUAUA ..((((.(((.....))).))))......((..(((((((((((((..(((.((((....((((.((((((.....))))))))))....))))......))).)))))))))))))..)).......................... (-30.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 330 100 51 124 73 pint:PI1924|5end_cytidine_deaminase_1865897..1866127_POSITIVE 302 99 53 122 65 pint:PI1633|5end_hypothetical_protein_1552504..1552734_POSITIVE 284 138 92 179 120 pint:PI1496|5end_nucleoside_phosphorylase_1417116..1417346_POSITIVE 283 90 45 223 159 pint:PI1660|5end_hypothetical_protein_1574659..1574889_POSITIVE 275 99 56 154 108 pint:PI0041|5end_hypothetical_protein_41785..42015_POSITIVE 267 87 41 220 170 pint:PI1099|5end_low_molecular_weight_protein-tyrosine-phosphatase_1023059..1023289_POSITIVE 266 80 46 225 193 pint:PI2160|5end_hypothetical_protein_2107270..2107500_POSITIVE 261 76 37 57 20 pint:PI0813|5end_conserved_hypothetical_protein_751095..751325_POSITIVE 260 85 52 79 31 pint:PI0480|5end_preprotein_translocase,_SecY_subunit_441576..441806_POSITIVE 258 140 96 69 20 pint:PI1550|5end_conserved_hypothetical_protein_1467360..1467590_POSITIVE 257 98 53 115 70 pint:PI2131|5end_hypothetical_protein_2080678..2080908_POSITIVE 257 95 51 56 12 pint:PI2105|5end_hypothetical_protein_2059357..2059587_POSITIVE 256 119 73 68 1 pint:PI0338|5end_hypothetical_protein_315337..315567_POSITIVE 252 98 51 231 178 pint:PI1659|5end_hypothetical_protein_1574725..1574955_POSITIVE 252 100 51 75 30 pint:PI1382|5end_hypothetical_protein_1303608..1303838_POSITIVE 250 48 2 83 38 pint:PI2075|5end_conserved_hypothetical_protein_2034421..2034651_POSITIVE 246 79 36 54 1 pint:PI1191|5end_phosphoribosyl_pyrophosphate_synthetase_1109464..1109694_POSITIVE 245 98 51 162 120 pint:PI0785|5end_hypothetical_protein_723744..723974_POSITIVE 244 97 51 59 11 pint:PI0814|5end_conserved_hypothetical_protein_752946..753176_POSITIVE 242 99 54 115 73 pint:PI1497|5end_LuxS_protein,_autoinducer_AI-2_production_protein_1417571..1417801_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 330 100 51 59 8 pint:PI1923|3end_conserved_hypothetical_protein_1865912..1866062_POSITIVE 271 94 55 63 8 pint:PI0857|3end_hypothetical_protein_790986..791136_POSITIVE 267 80 35 61 8 pint:PI1767|3end_fructokinase,_pfkB_family_1695243..1695393_POSITIVE 260 85 52 71 23 pint:PI0479|3end_50S_ribosomal_protein_L15_441648..441798_POSITIVE 259 50 2 88 46 pint:PI1083|3end_TPR-repeat-containing_protein_1002588..1002738_POSITIVE 259 87 41 94 40 pint:PI0778|3end_hypothetical_protein_714885..715035_POSITIVE 257 98 53 68 23 pint:PI2130|3end_DNA_polymerase_I_2080711..2080861_POSITIVE 257 95 51 117 73 pint:PI2106|3end_hypothetical_protein_2059498..2059648_POSITIVE 252 100 51 63 18 pint:PI1381|3end_conserved_hypothetical_protein_1303676..1303826_POSITIVE 242 100 53 129 80 pint:PI0955|3end_conserved_hypothetical_protein_886084..886234_POSITIVE 242 100 53 132 91 pint:PI0592|3end_DNA_mismatch_repair_protein_542611..542761_POSITIVE 240 78 46 77 42 pint:PI1667|3end_conserved_hypothetical_protein_1580772..1580922_POSITIVE 236 130 84 68 32 pint:PI0358|3end_hypothetical_protein_328105..328255_POSITIVE 234 76 33 57 6 pint:PI1960|3end_hypothetical_protein_1918983..1919133_POSITIVE 228 130 84 91 50 pint:PI1884|3end_conserved_hypothetical_protein_1827457..1827607_POSITIVE 228 90 44 117 70 pint:PI1269|3end_conserved_hypothetical_protein;_possible_peptidase_1188781..1188931_POSITIVE 227 130 86 135 89 pint:PI1025|3end_tRNA_modification_GTPase_942232..942382_POSITIVE 227 61 29 133 100 pint:PI0924|3end_possible_glycosyl_hydrolase_855953..856103_POSITIVE 225 139 112 145 111 pint:PI0679|3end_hypothetical_protein_617012..617162_POSITIVE 225 139 112 112 78 pint:PI0678|3end_hypothetical_protein_616979..617129_POSITIVE