Origin IGS: taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaaataatggcttttttattgtttcacgattctatttcaacggttcaatacaagtttttcaattaaacttttatacacgttttttaaggtttaaaaagcaaactgtaaagattatttaacagctacataatta Mask Tandem Repeat Region ================================================ taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Find is-nt database================================================ Query_seq: PI1019:PI1020|PI1019:PI1020:hypothetical protein:hypothetical protein:->->:938473..938608 136 taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1019:PI1020|PI1019:PI1020:hypothetical protein:hypothetical protein:->->:938473..938608 136 taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1019:PI1020|PI1019:PI1020:hypothetical protein:hypothetical protein:->->:938473..938608 136 taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 18 End: 116 ................. I I K V T Q K K F R L F R T Y F N L Q F V Q I S G N F Y F R S V M .................... taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 71 End: 100 ......................................................................ATTGAAAAACTTGTATTGAACCGTTGAAAT.................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1019:PI1020|PI1019:PI1020:hypothetical protein:hypothetical protein:->->:938473..938608 136 taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Intra-Species Hit: Count: 1 Min: 1 Max: 136 Len: 136 Subject: pint_PI1019_PI1020|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[938473,938608]|136 HSP 1 e-value: 1.0E-71 bit: 270.0 Len: 136 Query Start:1 Query End:136 Subject Strand: POSITIVE Subject Start: 1 Subject End: 136 taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca taattatgtagctgttaaataatctttacagtttgctttttaaaccttaaaaaacgtgtataaaagtttaattgaaaaacttgtattgaaccgttgaaatagaatcgtgaaacaataaaaaagccattatttttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUAUGUAGCUGUUAAAUAAUCUUUACAGUUUGCUUUUUAAACCUUAAAAAACGUGUAUAAAAGUUUAAUUGAAAAACUUGUAUUGAACCGUUGAAAUAGAAUCGUGAAACAAUAAAAAAGCCAUUAUUUUUCA ...(((((...(((((.(((..((..((((((((.....((((((....................)))))).....)))).))))..))...)))..)))))...))))).......................... (-15.05) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAAUAAUGGCUUUUUUAUUGUUUCACGAUUCUAUUUCAACGGUUCAAUACAAGUUUUUCAAUUAAACUUUUAUACACGUUUUUUAAGGUUUAAAAAGCAAACUGUAAAGAUUAUUUAACAGCUACAUAAUUA ..........(((((.((..((.((((...((.......)).((((((..(((.((((((.......)))))).)))....((((((((.....)))))))).))))))..)))).))..)).)))))........ (-16.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 418 120 75 176 131 pint:PI1302|5end_hypothetical_protein_1214797..1215027_POSITIVE 224 103 80 205 179 pint:PI1261|5end_ISPg3-related_transposase,_N-terminal_region_1183347..1183577_POSITIVE 208 130 83 204 162 pint:PI1952|5end_hypothetical_protein_1906588..1906818_POSITIVE 204 120 91 148 125 pint:PI0120|5end_glutamate_racemase_111778..112008_POSITIVE 203 115 80 223 179 pint:PI2110|5end_ISPg3-related_transposase_(IS195_family)_2061470..2061700_POSITIVE 197 59 24 198 159 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 194 120 80 137 97 pint:PI0134|5end_ISPg3-related_transposase_(IS5_family)_124721..124951_POSITIVE 190 36 3 222 185 pint:PI2166|5end_hypothetical_protein_2111394..2111624_POSITIVE 190 36 3 114 77 pint:PI2168|5end_hypothetical_protein_2111286..2111516_POSITIVE 190 130 84 227 189 pint:PI0044|5end_thiamine_biosynthetic_enzyme_42965..43195_POSITIVE 188 125 81 228 180 pint:PI1762|5end_ISPg3-related_transposase_1684669..1684899_POSITIVE 184 112 82 140 113 pint:PI1896|5end_hypothetical_protein_1840854..1841084_POSITIVE 181 125 82 196 159 pint:PI0675|5end_hypothetical_protein_613647..613877_POSITIVE 176 36 3 224 193 pint:PI0490|5end_DNA_primase_449070..449300_POSITIVE 176 115 71 99 55 pint:PI0171|5end_hypothetical_protein_163850..164080_POSITIVE 174 113 73 212 158 pint:PI0909|5end_possible_mutT/NUDIX_family_protein_837526..837756_POSITIVE 174 60 11 89 43 pint:PI0095|5end_hypothetical_protein_91444..91674_POSITIVE 174 114 84 121 92 pint:PI0042|5end_hypothetical_protein_42981..43211_POSITIVE 172 117 73 81 26 pint:PI1866|5end_hypothetical_protein_1811693..1811923_POSITIVE 172 120 79 115 71 pint:PI0640|5end_conserved_hypothetical_protein_583289..583519_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 227 115 71 46 2 pint:PI1608|3end_possible_Na+/H+_anti-porter_1536300..1536450_POSITIVE 224 103 80 50 24 pint:PI1260|3end_IS1001-related_transposase_1183272..1183422_POSITIVE 217 46 2 145 103 pint:PI0779|3end_predicted_TPR-repeat-containing_protein_715800..715950_POSITIVE 188 125 81 136 88 pint:PI1760|3end_hypothetical_protein_1684657..1684807_POSITIVE 186 60 11 129 76 pint:PI0778|3end_hypothetical_protein_714885..715035_POSITIVE 184 36 7 122 92 pint:PI0181|3end_conserved_hypothetical_protein_173940..174090_POSITIVE 180 110 70 57 12 pint:PI1499|3end_hypothetical_protein_1420180..1420330_POSITIVE 176 59 22 129 98 pint:PI2099|3end_D-alanyl-D-alanine_dipeptidase_2053277..2053427_POSITIVE 176 115 71 60 16 pint:PI0170|3end_hypothetical_protein_163891..164041_POSITIVE 174 60 11 102 56 pint:PI0093|3end_riboflavin_biosynthesis_protein_91537..91687_POSITIVE 172 47 7 101 56 pint:PI1797|3end_hypothetical_protein_1731559..1731709_POSITIVE 172 42 7 136 96 pint:PI1550|3end_conserved_hypothetical_protein_1467703..1467853_POSITIVE 171 119 79 126 90 pint:PI1396|3end_urocanate_hydratase_1317873..1318023_POSITIVE 166 111 85 55 23 pint:PI1864|3end_chromate_transport_protein_1811009..1811159_POSITIVE 164 95 56 70 33 pint:PI1233|3end_conserved_hypothetical_protein;_possible__MazG_family_protein_1154247..1154397_POSITIVE 163 96 65 124 90 pint:PI2159|3end_immunoreactive_84_kDa_antigen_PG93_2107371..2107521_POSITIVE 162 125 81 118 72 pint:PI0858|3end_carbamyl_phosphate_synthase,_large_subunit_793394..793544_POSITIVE 162 60 12 146 102 pint:PI0613|3end_glycerol-3-phosphate_dehydrogenase_(NAD(P)+)_563239..563389_POSITIVE 161 110 64 146 98 pint:PI1709|3end_ABC_transporter,_ATP-binding_protein_1626687..1626837_POSITIVE 161 40 1 146 104 pint:PI0467|3end_50S_ribosomal_protein_L16_436976..437126_POSITIVE