Origin IGS: caaatcttgccactttgaggtttcagcctctttaccgcatacacccttaatggtgcaacctgttccctttgcagtttcctgacactggaaacaaaacatcttattttctgtcataatgtttttatttaaatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaaacaaaaataaccttacatattttctaaaacaaaaatcatatttcagttacaaagataacaattaatagctaatagcacaaaaagtaatatacgaaaacagaaaaatatattagcaaataacaaatatataatactctgaaaactgtatgattgttacattctgattgcagaactacgttttaacaatactttaacagggcttagccacaaatgttaccagaccttaattcttatatcgatacctttgcatcagaaatttaaataaaaacattatgacagaaaataagatgttttgtttccagtgtcaggaaactgcaaagggaacaggttgcaccattaagggtgtatgcggtaaagaggctgaaacctcaaagtggcaagatttg Mask Tandem Repeat Region ================================================ caaatcttgccactttgaggtttcagcctctttaccgcatacacccttaatggtgcaacctgttccctttgcagtttcctgacactggaaacaaaacatcttattttctgtcataatgtttttatttaaatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Find is-nt database================================================ Query_seq: PI1014:PI1015|PI1014:PI1015:prismane protein, hybrid-cluster protein:conserved hypothetical protein:->->:936193..936582 390 caaatcttgccactttgaggtttcagcctctttaccgcatacacccttaatggtgcaacctgttccctttgcagtttcctgacactggaaacaaaacatcttattttctgtcataatgtttttatttaaatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1014:PI1015|PI1014:PI1015:prismane protein, hybrid-cluster protein:conserved hypothetical protein:->->:936193..936582 390 caaatcttgccactttgaggtttcagcctctttaccgcatacacccttaatggtgcaacctgttccctttgcagtttcctgacactggaaacaaaacatcttattttctgtcataatgtttttatttaaatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1014:PI1015|PI1014:PI1015:prismane protein, hybrid-cluster protein:conserved hypothetical protein:->->:936193..936582 390 caaatcttgccactttgaggtttcagcctctttaccgcatacacccttaatggtgcaacctgttccctttgcagtttcctgacactggaaacaaaacatcttattttctgtcataatgtttttatttaaatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 9 Min: 1 Max: 129 Len: 129 Subject: UniRef90_Q8RFL1 Cluster: Hydroxylamine reductase; n=2; Fusobacterium nucleatum|Rep: Hydroxylamine reductase - Fusobacterium nucleatum subsp. nucleatum HSP 1 e-value: 8.0E-9 bit: 61.6 Len: 105 Query Start:1 Query End:105 Subject Strand: null Subject Start: 2 Subject End: 36 N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L ............................................................................................................................................................................................................................................................................................. D K M F C Y Q C Q E T A K G T G C T S I G V C G K T S E T S G L Q D L ............................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q2WTU5 Cluster: Hybrid cluster protein; n=1; Clostridium beijerincki NCIMB 8052|Rep: Hybrid cluster protein - Clostridium beijerincki NCIMB 8052 HSP 1 e-value: 2.0E-8 bit: 60.1 Len: 108 Query Start:1 Query End:108 Subject Strand: null Subject Start: 2 Subject End: 37 E N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L .......................................................................................................................................................................................................................................................................................... D N K M F C F Q C Q E A A G C T G C T V K G V C G K T P D L A K M Q D L .......................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q1FFP0 Cluster: Hybrid cluster protein; n=1; Clostridium phytofermentans ISDg|Rep: Hybrid cluster protein - Clostridium phytofermentans ISDg HSP 1 e-value: 8.0E-7 bit: 55.1 Len: 129 Query Start:1 Query End:129 Subject Strand: null Subject Start: 1 Subject End: 43 L N K N I M T E N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L ..................................................................................................................................................................................................................................................................... M G N N M D L E Y E M F C Y Q C E Q T A G G K G C T K Q G V C G K T A E I A N L Q D L ..................................................................................................................................................................................................................................................................... Subject: UniRef90_Q30Y09 Cluster: Hybrid cluster protein; n=1; Desulfovibrio desulfuricans G20|Rep: Hybrid cluster protein - Desulfovibrio desulfuricans (strain G20) HSP 1 e-value: 1.0E-6 bit: 54.3 Len: 105 Query Start:4 Query End:108 Subject Strand: null Subject Start: 36 Subject End: 70 ... E N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D .......................................................................................................................................................................................................................................................................................... ... E Y S M F C N Q C E Q T A K G T G C T A M G V C G K Q P E V S D L Q D .......................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q2WSD7 Cluster: Hybrid cluster protein; n=1; Clostridium beijerincki NCIMB 8052|Rep: Hybrid cluster protein - Clostridium beijerincki NCIMB 8052 HSP 1 e-value: 1.0E-6 bit: 54.3 Len: 114 Query Start:1 Query End:114 Subject Strand: null Subject Start: 1 Subject End: 38 M T E N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L .................................................................................................................................................................................................................................................................................... M D E N P M F C Y Q C E Q T A G G K G C T K M G V C G K T P E I A N L Q D L .................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q7MVY0 Cluster: Hydroxylamine reductase; n=1; Porphyromonas gingivalis|Rep: Hydroxylamine reductase - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 2.0E-6 bit: 53.9 Len: 108 Query Start:1 Query End:108 Subject Strand: null Subject Start: 2 Subject End: 37 E N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L .......................................................................................................................................................................................................................................................................................... E K K M F C Y Q C Q E T A G N K G C I L K G V C G K D F S T A N L M D L .......................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q1FFN9 Cluster: Hybrid cluster protein; n=1; Clostridium phytofermentans ISDg|Rep: Hybrid cluster protein - Clostridium phytofermentans ISDg HSP 1 e-value: 3.0E-6 bit: 53.1 Len: 105 Query Start:1 Query End:105 Subject Strand: null Subject Start: 2 Subject End: 36 N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L ............................................................................................................................................................................................................................................................................................. S Q M F C F Q C Q E T A G N K G C T L N G V C G K T A A L A N M Q D L ............................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q8R6M9 Cluster: Hydroxylamine reductase; n=3; Thermoanaerobacter|Rep: Hydroxylamine reductase - Thermoanaerobacter tengcongensis HSP 1 e-value: 3.0E-6 bit: 53.1 Len: 102 Query Start:1 Query End:102 Subject Strand: null Subject Start: 2 Subject End: 35 K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L ................................................................................................................................................................................................................................................................................................ K M F C Y Q C Q E A L K N T G C T T I G V C G K T A D V A N L Q D L ................................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q2PCB1 Cluster: Hybrid-cluster protein; n=1; Naegleria gruberi|Rep: Hybrid-cluster protein - Naegleria gruberi HSP 1 e-value: 6.0E-6 bit: 52.0 Len: 105 Query Start:1 Query End:105 Subject Strand: null Subject Start: 5 Subject End: 39 N K M F C F Q C Q E T A K G T G C T I K G V C G K E A E T S K W Q D L ............................................................................................................................................................................................................................................................................................. D K M F C F Q C E Q T Q N G T G C T T V G V C G K T P S V A A L Q D L ............................................................................................................................................................................................................................................................................................. nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 217 End: 315 ........................................................................................................................................................................................................................ M Q S E C N N H T V F R V L Y I C Y L L I Y F S V F V Y Y F L C Y ........................................................................... Protein_Len: 40 Strand: - Start: 125 End: 244 ............................................................................................................................ K F K Q H L P I S I L I L T Q Y C K H S L G T L T N N F R L E A I L I Y C D Y M .................................................................................................................................................. Protein_Len: 34 Strand: - Start: 3 End: 104 .. I K G S Q P K L R K V A Y V R L P A V Q E R Q L K R V S S V F C R M .............................................................................................................................................................................................................................................................................................. Protein_Len: 46 Strand: - Start: 1 End: 138 L D Q W K S T E A E K G C V G K I T C G T G K A T E Q C Q F C F M K N E T M I N K N L N R M ............................................................................................................................................................................................................................................................ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1014:PI1015|PI1014:PI1015:prismane protein, hybrid-cluster protein:conserved hypothetical protein:->->:936193..936582 390 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacagttttcagagtattatatatttgttatttgctaatatatttttctgttttcgtatattactttttgtgctattagctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Intra-Species Hit: Count: 1 Min: 130 Max: 390 Len: 261 Subject: pint_PI1014_PI1015|prismane protein, hybrid-cluster protein:conserved hypothetical protein|POSITIVE:POSITIVE|[936193,936582]|390 HSP 1 e-value: 1.0E-58 bit: 228.0 Len: 115 Query Start:130 Query End:244 Subject Strand: POSITIVE Subject Start: 130 Subject End: 244 .................................................................................................................................atttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacag.................................................................................................................................................. .................................................................................................................................atttctgatgcaaaggtatcgatataagaattaaggtctggtaacatttgtggctaagccctgttaaagtattgttaaaacgtagttctgcaatcagaatgtaacaatcatacag.................................................................................................................................................. HSP 2 e-value: 1.0E-33 bit: 145.0 Len: 73 Query Start:318 Query End:390 Subject Strand: POSITIVE Subject Start: 318 Subject End: 390 .............................................................................................................................................................................................................................................................................................................................gctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa .............................................................................................................................................................................................................................................................................................................................gctattaattgttatctttgtaactgaaatatgatttttgttttagaaaatatgtaaggttatttttgtttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUCUGAUGCAAAGGUAUCGAUAUAAGAAUUAAGGUCUGGUAACAUUUGUGGCUAAGCCCUGUUAAAGUAUUGUUAAAACGUAGUUCUGCAAUCAGAAUGUAACAAUCAUACAGUUUUCAGAGUAUUAUAUAUUUGUUAUUUGCUAAUAUAUUUUUCUGUUUUCGUAUAUUACUUUUUGUGCUAUUAGCUAUUAAUUGUUAUCUUUGUAACUGAAAUAUGAUUUUUGUUUUAGAAAAUAUGUAAGGUUAUUUUUGUUUAA .........(((((((((...(((((.............(((((((...((((((((((.(....((((((((((((.......((((((....)))))).))))))..((((.(....(((((....(((((((.((.....)).)))))))..)))))....)))))..))))))..).)))..)))))))....))))))).......((((((((.......))))))))....))))).....))))))))).... (-51.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAAACAAAAAUAACCUUACAUAUUUUCUAAAACAAAAAUCAUAUUUCAGUUACAAAGAUAACAAUUAAUAGCUAAUAGCACAAAAAGUAAUAUACGAAAACAGAAAAAUAUAUUAGCAAAUAACAAAUAUAUAAUACUCUGAAAACUGUAUGAUUGUUACAUUCUGAUUGCAGAACUACGUUUUAACAAUACUUUAACAGGGCUUAGCCACAAAUGUUACCAGACCUUAAUUCUUAUAUCGAUACCUUUGCAUCAGAAAU ...............................((((...((((((...(((((...................((.....)).....................((((...(((((((.(.......).))))))).....))))..)))))))))))))))...(((((((.(((((.....((....)).......(((((.(((...))).....)))))...........................)))))))))))).. (-32.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 191 152 153 111 pint:PI0333|5end_hypothetical_protein_313478..313708_POSITIVE 281 49 4 212 168 pint:PI1577|5end_conserved_hypothetical_protein_1503602..1503832_POSITIVE 253 208 162 116 62 pint:PI0514|5end_possible_cell_filamentation_protein_472408..472638_POSITIVE 249 25 2 148 124 pint:PI1504|5end_non-specific_DNA-binding_protein_1424318..1424548_POSITIVE 246 58 14 64 22 pint:PI0518|5end_5'-nucleotidase_precursor_477076..477306_POSITIVE 244 98 51 74 19 pint:PI1812|5end_possible_alpha-amylase_1750759..1750989_POSITIVE 243 62 21 159 118 pint:PI1419|5end_conserved_hypothetical_protein;_membrane_protease_subunits,_stomatin/prohibitin_homologs_1339136..1339366_POSITIVE 240 90 45 135 89 pint:PI1994|5end_hypothetical_protein_1949360..1949590_POSITIVE 240 125 82 77 29 pint:PI0861|5end_glucosamine--fructose-6-phosphate_aminotransferase_(glutamine-fructose-6-phosphate_transaminase_(isomerizing))_796346..796576_POSITIVE 236 97 52 50 7 pint:PI1156|5end_possible_glycerate_kinase_family_protein_1080583..1080813_POSITIVE 236 197 157 41 9 pint:PI0910|5end_possible_sodium_hydrogen_antiporter_(Na+/H+_antiporter)_838085..838315_POSITIVE 234 55 15 231 199 pint:PI1744|5end_chromosome_partitioning_protein_1665635..1665865_POSITIVE 231 69 26 182 134 pint:PI1790|5end_hypothetical_protein_1722179..1722409_POSITIVE 229 85 57 231 205 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 229 74 32 82 50 pint:PI0680|5end_hypothetical_protein_616958..617188_POSITIVE 227 119 81 86 50 pint:PI0813|5end_conserved_hypothetical_protein_751095..751325_POSITIVE 224 190 141 95 48 pint:PI1653|5end_hypothetical_protein_1565158..1565388_POSITIVE 224 59 14 133 77 pint:PI1258|5end_elongation_factor_Tu_1178909..1179139_POSITIVE 224 60 12 131 80 pint:PI0181|5end_conserved_hypothetical_protein_173435..173665_POSITIVE 219 90 45 46 2 pint:PI1782|5end_possible_LPS_biosynthesis_protein_1714482..1714712_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 191 152 147 105 pint:PI0332|3end_conserved_hypothetical_protein_313552..313702_POSITIVE 243 62 21 144 103 pint:PI1418|3end_UDP-N-acetylglucosamine_2-epimerase_1339201..1339351_POSITIVE 236 60 20 87 44 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 233 216 177 114 74 pint:PI0572|3end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_517589..517739_POSITIVE 231 77 36 60 12 pint:PI0758|3end_hypothetical_protein_690506..690656_POSITIVE 229 74 32 56 24 pint:PI0679|3end_hypothetical_protein_617012..617162_POSITIVE 224 190 141 92 45 pint:PI1652|3end_hypothetical_protein_1565235..1565385_POSITIVE 218 100 53 63 18 pint:PI1010|3end_conserved_hypothetical_protein_932195..932345_POSITIVE 217 100 59 143 93 pint:PI1004|3end_conserved_hypothetical_protein_926217..926367_POSITIVE 216 99 58 125 86 pint:PI2039|3end_hypothetical_protein_1992991..1993141_POSITIVE 216 61 32 102 75 pint:PI1417|3end_MATE_efflux_family_protein_(Na+-driven_multidrug_efflux_pump)_1337501..1337651_POSITIVE 216 37 6 44 19 pint:PI1384|3end_conserved_hypothetical_protein_1306174..1306324_POSITIVE 216 92 53 118 94 pint:PI1195|3end_hypothetical_protein_1113460..1113610_POSITIVE 214 210 169 147 104 pint:PI2030|3end_hypothetical_protein_1986127..1986277_POSITIVE 214 167 121 125 70 pint:PI1345|3end_conserved_hypothetical_protein;_possible_TPR-repeat-containing_protein_1260680..1260830_POSITIVE 210 60 13 64 8 pint:PI0406|3end_hypothetical_protein_373053..373203_POSITIVE 210 180 133 103 47 pint:PI0248|3end_protein_associated_with_conjugate_transformation/chromosome_partioning_246647..246797_POSITIVE 209 82 43 151 110 pint:PI0682|3end_hypothetical_protein_619372..619522_POSITIVE 208 209 178 39 2 pint:PI1658|3end_conserved_hypothetical_protein_1574736..1574886_POSITIVE 207 190 169 141 119 pint:PI1989|3end_conserved_hypothetical_protein;_possible_thioredoxin_1944583..1944733_POSITIVE