Origin IGS: taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acatagcccgataatacagatgagtaaatgctttttcatactaaactcctttctttaaattattcatagctacatcaatactattatttgtagcaaatttaggcattaaaatccataaatccaaatttattcataccttcttaacaaatccgtatttttataggaatatctatcaacaacaagtgttaaaaaggttcattctttttgtaaaactttctttacgctgcttta Mask Tandem Repeat Region ================================================ taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Find is-nt database================================================ Query_seq: PI0995:PI0997|PI0995:PI0997:hypothetical protein:hypothetical protein:->->:920884..921114 231 taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0995:PI0997|PI0995:PI0997:hypothetical protein:hypothetical protein:->->:920884..921114 231 taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0995:PI0997|PI0995:PI0997:hypothetical protein:hypothetical protein:->->:920884..921114 231 taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Predict ORF larger than 30AA ================================================ Protein_Len: 55 Strand: - Start: 2 End: 166 . F C R L S L K V F L I F R K L V Q Q Q Y I N R Y F Y P N T L F T H I F K S K H I K I G L N A V F L L I S T A M ................................................................. taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 192 End: 221 ...............................................................................................................................................................................................TATGAAAAAGCATTTACTCATCTGTATTAT.......... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0995:PI0997|PI0995:PI0997:hypothetical protein:hypothetical protein:->->:920884..921114 231 taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Intra-Species Hit: Count: 1 Min: 1 Max: 231 Len: 231 Subject: pint_PI0995_PI0997|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[920884,921114]|231 HSP 1 e-value: 1.0E-128 bit: 458.0 Len: 231 Query Start:1 Query End:231 Subject Strand: POSITIVE Subject Start: 1 Subject End: 231 taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt taaagcagcgtaaagaaagttttacaaaaagaatgaacctttttaacacttgttgttgatagatattcctataaaaatacggatttgttaagaaggtatgaataaatttggatttatggattttaatgcctaaatttgctacaaataatagtattgatgtagctatgaataatttaaagaaaggagtttagtatgaaaaagcatttactcatctgtattatcgggctatgt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGCAGCGUAAAGAAAGUUUUACAAAAAGAAUGAACCUUUUUAACACUUGUUGUUGAUAGAUAUUCCUAUAAAAAUACGGAUUUGUUAAGAAGGUAUGAAUAAAUUUGGAUUUAUGGAUUUUAAUGCCUAAAUUUGCUACAAAUAAUAGUAUUGAUGUAGCUAUGAAUAAUUUAAAGAAAGGAGUUUAGUAUGAAAAAGCAUUUACUCAUCUGUAUUAUCGGGCUAUGU ...(((...((((((....))))))...........((((((((((((.((((.(((.((((......))))...)))))))...)))))))))))).............(((.(((((((...(((((((((((((((((((((((....))))..))))))..(((.....))).......)))))))).........))))).....)))))))..)))..))).... (-38.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUAGCCCGAUAAUACAGAUGAGUAAAUGCUUUUUCAUACUAAACUCCUUUCUUUAAAUUAUUCAUAGCUACAUCAAUACUAUUAUUUGUAGCAAAUUUAGGCAUUAAAAUCCAUAAAUCCAAAUUUAUUCAUACCUUCUUAACAAAUCCGUAUUUUUAUAGGAAUAUCUAUCAACAACAAGUGUUAAAAAGGUUCAUUCUUUUUGUAAAACUUUCUUUACGCUGCUUUA ...((((..((((......((((((((((.......................................((((((..............))))))..(((((((.((....)))).)))))....))))))))))..............(((.(((....)))))).....))))......((((..((((((((.....))))))))....)))).......))))..... (-29.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 271 193 151 116 63 pint:PI0305|5end_possible_aminotransferase_287718..287948_POSITIVE 253 140 91 220 176 pint:PI1795|5end_conserved_hypothetical_protein_1726864..1727094_POSITIVE 252 119 79 101 60 pint:PI1026|5end_uridine_phosphorylase_942300..942530_POSITIVE 247 230 201 150 111 pint:PI1663|5end_hypothetical_protein_1577127..1577357_POSITIVE 233 230 182 193 149 pint:PI1528|5end_hypothetical_protein_1449685..1449915_POSITIVE 225 226 182 69 29 pint:PI0388|5end_integrase_354927..355157_POSITIVE 222 183 148 76 39 pint:PI1405|5end_conserved_hypothetical_protein_1326035..1326265_POSITIVE 221 225 183 117 68 pint:PI0741|5end_conserved_hypothetical_protein_672487..672717_POSITIVE 219 226 183 207 171 pint:PI2054|5end_hypothetical_protein_2012883..2013113_POSITIVE 219 230 183 161 122 pint:PI1451|5end_outer_membrane_receptor_proteins,_tonB_dependent_(possibly_iron_transport_related)_1372931..1373161_POSITIVE 216 73 38 72 41 pint:PI0385|5end_hypothetical_protein_353968..354198_POSITIVE 214 226 182 188 142 pint:PI2104|5end_carboxyl-terminal_protease_2057910..2058140_POSITIVE 212 229 181 225 169 pint:PI1510|5end_cell_division_protein_1428412..1428642_POSITIVE 211 230 193 64 24 pint:PI0049|5end_outer_membrane_receptor_protein_(iron_transport)_48605..48835_POSITIVE 208 73 41 140 109 pint:PI0943|5end_conserved_hypothetical_protein_875153..875383_POSITIVE 208 73 41 55 24 pint:PI0944|5end_hypothetical_protein_875068..875298_POSITIVE 205 230 187 53 2 pint:PI2052|5end_hypothetical_protein_2008594..2008824_POSITIVE 203 219 173 78 37 pint:PI1208|5end_monofunctional_biosynthetic_peptidoglycan_transglycosylase_1127155..1127385_POSITIVE 203 189 144 133 90 pint:PI0605|5end_chloromuconate_cycloisomerase_551912..552142_POSITIVE 203 230 182 227 176 pint:PI0519|5end_5'-nucleotidase/2',3'-cyclic_phosphodiesterase_477953..478183_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 250 140 91 131 76 pint:PI1099|3end_low_molecular_weight_protein-tyrosine-phosphatase_1023636..1023786_POSITIVE 219 226 183 120 84 pint:PI2053|3end_conserved_hypothetical_protein;_possible_outer_membrane_receptor_protein_2012876..2013026_POSITIVE 219 230 183 41 2 pint:PI1450|3end_hypothetical_protein_1372891..1373041_POSITIVE 214 226 182 132 86 pint:PI2103|3end_hypothetical_protein_2057934..2058084_POSITIVE 211 229 181 143 99 pint:PI1509|3end_conserved_hypothetical_protein_1428422..1428572_POSITIVE 203 219 173 83 42 pint:PI1205|3end_hypothetical_protein_1127240..1127390_POSITIVE 203 219 173 52 11 pint:PI1207|3end_hypothtical_protein_1127209..1127359_POSITIVE 203 189 144 59 16 pint:PI0604|3end_O-succinylbenzoic_acid--CoA_ligase_551918..552068_POSITIVE 203 79 49 69 40 pint:PI0449|3end_hypothetical_protein_423727..423877_POSITIVE 197 65 24 84 49 pint:PI1889|3end_UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape_ptide)_pyrophosphoryl-undecaprenol_N-acetylglucosamine_transferase_1832591..1832741_POSITIVE 196 230 181 106 65 pint:PI1864|3end_chromate_transport_protein_1811009..1811159_POSITIVE 188 140 92 151 106 pint:PI1787|3end_conserved_hypothetical_protein_1719165..1719315_POSITIVE 187 111 77 150 112 pint:PI0878|3end_hypothetical_protein_813489..813639_POSITIVE 186 230 189 129 85 pint:PI1561|3end_conserved_hypothetical_protein;_possible_lipoprotein_1479702..1479852_POSITIVE 186 119 76 113 82 pint:PI1055|3end_aspartokinase_976302..976452_POSITIVE 186 230 200 42 14 pint:PI0358|3end_hypothetical_protein_328105..328255_POSITIVE 184 141 105 128 93 pint:PI0190|3end_3-dehydroquinate_dehydratase_II_181126..181276_POSITIVE 183 218 177 112 76 pint:PI0535|3end_hypothetical_protein_489779..489929_POSITIVE 183 226 182 147 102 pint:PI0518|3end_5'-nucleotidase_precursor_477959..478109_POSITIVE 183 138 107 60 27 pint:PI0484|3end_30S_ribosomal_protein_S11_444488..444638_POSITIVE