Origin IGS: aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggcgaataaaacagttctaaaagaaaataaaaatgcccaacgcttcgcagcgacgggcactcaacctcaataggtattagtcatttaaggtaaaaagattgtattcaggataacgtgtgcaaagatagcttaaattctattatctaccaaatttttttttaattatttttcaaaaatatacgatgcaagtataaaagtgctttaagatttacaacaaaagaaacattatgcaacactttatggttatcaaattacattattaataataaaaacatataataagaagaaaaaaataactatctttgcagaagaaaaaattatagttt Mask Tandem Repeat Region ================================================ aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Find is-nt database================================================ Query_seq: PI0994:PI0996|PI0994:PI0996:glutaminyl-tRNA synthetase:hypothetical protein:->->:920270..920597 328 aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0994:PI0996|PI0994:PI0996:glutaminyl-tRNA synthetase:hypothetical protein:->->:920270..920597 328 aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0994:PI0996|PI0994:PI0996:glutaminyl-tRNA synthetase:hypothetical protein:->->:920270..920597 328 aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 51 End: 149 .................................................. I N K N N I I Y N S L W L T N C L T E K T T F R L A S K Y K C R M ................................................................................................................................................................................... aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 164 End: 193 ...................................................................................................................................................................ATTAAAAAAAAATTTGGTAGATAATAGAAT....................................................................................................................................... PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 176 End: 205 ...............................................................................................................................................................................TTTGGTAGATAATAGAATTTAAGCTATCTT........................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.1 Tail_Score: -5.70761 Start: 268 End: 293 Full_Region: CCTATTGAGGTTGAG TGCCCGTCGCT GCGA AGCGTTGGGCA TTTTTATTTTCTTTT ...........................................................................................................................................................................................................................................................................TGCCCGTCGCTGCGAAGCGTTGGGCA................................... Find igs database================================================ Query_seq: PI0994:PI0996|PI0994:PI0996:glutaminyl-tRNA synthetase:hypothetical protein:->->:920270..920597 328 aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Intra-Species Hit: Count: 1 Min: 1 Max: 328 Len: 328 Subject: pint_PI0994_PI0996|glutaminyl-tRNA synthetase:hypothetical protein|POSITIVE:POSITIVE|[920270,920597]|328 HSP 1 e-value: 1.0E-157 bit: 555.0 Len: 328 Query Start:1 Query End:328 Subject Strand: POSITIVE Subject Start: 1 Subject End: 328 aaactataattttttcttctgcaaagatagttannnnnnncttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattnnnnnnnnntttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta aaactataattttttcttctgcaaagatagttatttttttcttcttattatatgtttttattattaataatgtaatttgataaccataaagtgttgcataatgtttcttttgttgtaaatcttaaagcacttttatacttgcatcgtatatttttgaaaaataattaaaaaaaaatttggtagataatagaatttaagctatctttgcacacgttatcctgaatacaatctttttaccttaaatgactaatacctattgaggttgagtgcccgtcgctgcgaagcgttgggcatttttattttcttttagaactgttttattcgccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAACUAUAAUUUUUUCUUCUGCAAAGAUAGUUAUUUUUUUCUUCUUAUUAUAUGUUUUUAUUAUUAAUAAUGUAAUUUGAUAACCAUAAAGUGUUGCAUAAUGUUUCUUUUGUUGUAAAUCUUAAAGCACUUUUAUACUUGCAUCGUAUAUUUUUGAAAAAUAAUUAAAAAAAAAUUUGGUAGAUAAUAGAAUUUAAGCUAUCUUUGCACACGUUAUCCUGAAUACAAUCUUUUUACCUUAAAUGACUAAUACCUAUUGAGGUUGAGUGCCCGUCGCUGCGAAGCGUUGGGCAUUUUUAUUUUCUUUUAGAACUGUUUUAUUCGCCUA ...................((((((((((((((..((((...(((.((((.((.(((((((((((((.((((((...((........((((((((...(((.(((((.......).)))).))).))))))))........))...)))))).))))....))).))))))...)).)))))))....))))..).)))))))))))))...........(((((.(((..(((((....((((((.....((((....)))).(((((((((.((((....)))).))))))))))))))).....)))))..))).)))))..... (-61.99) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGCGAAUAAAACAGUUCUAAAAGAAAAUAAAAAUGCCCAACGCUUCGCAGCGACGGGCACUCAACCUCAAUAGGUAUUAGUCAUUUAAGGUAAAAAGAUUGUAUUCAGGAUAACGUGUGCAAAGAUAGCUUAAAUUCUAUUAUCUACCAAAUUUUUUUUUAAUUAUUUUUCAAAAAUAUACGAUGCAAGUAUAAAAGUGCUUUAAGAUUUACAACAAAAGAAACAUUAUGCAACACUUUAUGGUUAUCAAAUUACAUUAUUAAUAAUAAAAACAUAUAAUAAGAAGAAAAAAAUAACUAUCUUUGCAGAAGAAAAAAUUAUAGUUU ..........((((.....(((((((((((.....(((((..((((....))))..)))))...........((((((.(((..(((((((.((......((((((...(.....)..))))))...)).))))))).))).))))))....)))))))))))....((((((.....(((((.......)))))...................................(((((......((((((((.........((((((..............))))))..........))))))))..)))))...))))))......)))) (-51.35) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 395 291 253 43 10 pint:PI1811|5end_conserved_hypothetical_protein_1749518..1749748_POSITIVE 367 284 246 127 84 pint:PI0748|5end_possible_mobilization_protein_676616..676846_POSITIVE 361 290 244 155 105 pint:PI0758|5end_hypothetical_protein_690247..690477_POSITIVE 349 288 251 211 178 pint:PI0932|5end_conserved_hypothetical_protein;_possible_hemin_receptor_863854..864084_POSITIVE 345 295 267 35 4 pint:PI1764|5end_conserved_hypothetical_protein_1688948..1689178_POSITIVE 333 292 251 61 19 pint:PI1092|5end_conserved_hypothetical_protein_1016780..1017010_POSITIVE 328 310 262 175 130 pint:PI0337|5end_hypothetical_protein_314732..314962_POSITIVE 324 297 254 94 34 pint:PI0545|5end_sugar_kinase_496411..496641_POSITIVE 322 293 253 185 134 pint:PI0262|5end_conserved_hypothetical_protein_254685..254915_POSITIVE 318 285 243 226 176 pint:PI0181|5end_conserved_hypothetical_protein_173435..173665_POSITIVE 317 292 251 66 16 pint:PI1150|5end_30S_ribosomal_protein_S6_1075683..1075913_POSITIVE 316 292 254 97 58 pint:PI0141|5end_glutamine_amidotransferase_involved_in_pyridoxine_biosynthesis_132710..132940_POSITIVE 315 293 259 182 143 pint:PI1180|5end_ferric_uptake_regulation_protein_1101892..1102122_POSITIVE 315 279 235 69 25 pint:PI0479|5end_50S_ribosomal_protein_L15_441125..441355_POSITIVE 311 295 254 196 142 pint:PI1000|5end_conserved_hypothetical_protein_923332..923562_POSITIVE 309 294 261 51 15 pint:PI2086|5end_signal_peptidase_I_2041835..2042065_POSITIVE 309 292 265 144 108 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 308 291 267 34 7 pint:PI1782|5end_possible_LPS_biosynthesis_protein_1714482..1714712_POSITIVE 306 294 253 113 70 pint:PI2062|5end_type_I_restriction-modification_system_2021658..2021888_POSITIVE 306 292 253 87 42 pint:PI0068|5end_arginine_repressor_66586..66816_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 284 246 133 90 pint:PI0747|3end_possible_mobilization_protein_676702..676852_POSITIVE 344 293 253 73 32 pint:PI0675|3end_hypothetical_protein_613957..614107_POSITIVE 329 292 253 50 13 pint:PI0999|3end_hypothetical_protein_922812..922962_POSITIVE 327 310 267 114 66 pint:PI1947|3end_hypothetical_protein_1902865..1903015_POSITIVE 318 285 243 141 91 pint:PI0180|3end_TonB_protein_173430..173580_POSITIVE 317 292 251 60 10 pint:PI1149|3end_30S_ribosomal_protein_S18_1075757..1075907_POSITIVE 311 295 254 120 66 pint:PI0998|3end_possible_flavin_reductase_domain_protein_923336..923486_POSITIVE 308 307 261 138 93 pint:PI1227|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148974..1149124_POSITIVE 307 295 259 117 85 pint:PI1146|3end_translation_initiation_factor_IF3_1073503..1073653_POSITIVE 304 295 259 113 61 pint:PI2008|3end__ABC_transporter,_ATP-binding_protein_1967961..1968111_POSITIVE 303 293 253 82 38 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 301 292 253 109 60 pint:PI1568|3end_OmpA_family_protein_1491043..1491193_POSITIVE 301 283 247 67 21 pint:PI0939|3end_TonB-dependent_outer_membrane_protein_872441..872591_POSITIVE 290 292 254 61 8 pint:PI1534|3end_ATPase/GTPase_1454166..1454316_POSITIVE 288 290 250 126 71 pint:PI1623|3end_hypothetical_protein_1549261..1549411_POSITIVE 288 290 250 126 71 pint:PI0330|3end_hypothetical_protein_312545..312695_POSITIVE 287 295 255 148 104 pint:PI0407|3end_tryptophanyl-tRNA_synthetase_(tryptophan--tRNA_ligase)_374147..374297_POSITIVE 285 295 255 66 15 pint:PI0788|3end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726789..726939_POSITIVE 284 298 259 148 106 pint:PI1230|3end_probable_metallo-beta-lactamase_family_protein_1152729..1152879_POSITIVE 283 298 256 124 81 pint:PI1135|3end_hypothetical_protein_1062203..1062353_POSITIVE