Origin IGS: tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatttacttacacgtaaagaaatatttatttacacgtagaaaaatatttctttacgtgtaaataatttatagaagttttggttttacaccataaaaaatacttgaacataccctatttgcctatccaagacggagaacctaataatgaacgagaaggtagatttacccatttttaggta Mask Tandem Repeat Region ================================================ tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnta Find is-nt database================================================ Query_seq: PI0982:PI0983|PI0982:PI0983:hypothetical protein:hypothetical protein:->->:909710..909889 180 tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0982:PI0983|PI0982:PI0983:hypothetical protein:hypothetical protein:->->:909710..909889 180 tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0982:PI0983|PI0982:PI0983:hypothetical protein:hypothetical protein:->->:909710..909889 180 tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 7 End: 111 ...... F H T F R G E R E N N P E G D Q I P L Y P I N L Y K K H H L V L V E M ..................................................................... tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 71 HP_score: -5.7 Tail_Score: -4.48299 Start: 112 End: 157 Full_Region: AACCAAAACTTCTAT AAATTATTTACACGTAAAGAA ATAT TTTTCTACGTGTAAATAAATA TTTCTTTACGTGTAA ...............................................................................................................AAATTATTTACACGTAAAGAAATATTTTTCTACGTGTAAATAAATA....................... TransTerm Strand: + Conf: 77 HP_score: -8.3 Tail_Score: -3.84346 Start: 114 End: 155 Full_Region: CCAAAACTTCTATAA ATTATTTACACGTAAAGAA ATAT TTTTCTACGTGTAAATAAA TATTTCTTTACGTGT .................................................................................................................ATTATTTACACGTAAAGAAATATTTTTCTACGTGTAAATAAA......................... TransTerm Strand: - Conf: 100 HP_score: -11.8 Tail_Score: -3.92143 Start: 115 End: 154 Full_Region: cacgtaaagaaatat ttatttacacgtagaaaa atat ttctttacgtgtaaataa tttatagaagttttg ..................................................................................................................ttatttacacgtagaaaaatatttctttacgtgtaaataa.......................... TransTerm Strand: - Conf: 100 HP_score: -16.0 Tail_Score: -3.0169 Start: 118 End: 173 Full_Region: taattta cttacacgtaaagaaatatttattta cacg tagaaaaatatttctttacgtgtaaa taatttatagaagtt .....................................................................................................................cttacacgtaaagaaatatttatttacacgtagaaaaatatttctttacgtgtaaa....... Find igs database================================================ Query_seq: PI0982:PI0983|PI0982:PI0983:hypothetical protein:hypothetical protein:->->:909710..909889 180 tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta Intra-Species Hit: Count: 1 Min: 1 Max: 180 Len: 180 Subject: pint_PI0982_PI0983|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[909710,909889]|180 HSP 1 e-value: 8.0E-98 bit: 357.0 Len: 180 Query Start:1 Query End:180 Subject Strand: POSITIVE Subject Start: 1 Subject End: 180 tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta tacctaaaaatgggtaaatctaccttctcgttcattattaggttctccgtcttggataggcaaatagggtatgttcaagtattttttatggtgtaaaaccaaaacttctataaattatttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaagtaaatta HSP 2 e-value: 3.0E-8 bit: 60.0 Len: 54 Query Start:119 Query End:172 Subject Strand: NEGATIVE Subject Start: 119 Subject End: 172 ......................................................................................................................ttacacgtaaagaaatatttttctacgtgtaaataaatatttctttacgtgtaa........ ......................................................................................................................ttacacgtaaagaaatatttatttacacgtagaaaaatatttctttacgtgtaa........ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACCUAAAAAUGGGUAAAUCUACCUUCUCGUUCAUUAUUAGGUUCUCCGUCUUGGAUAGGCAAAUAGGGUAUGUUCAAGUAUUUUUUAUGGUGUAAAACCAAAACUUCUAUAAAUUAUUUACACGUAAAGAAAUAUUUUUCUACGUGUAAAUAAAUAUUUCUUUACGUGUAAGUAAAUUA .((((((.((((((((....))))........)))).)))))).......((((((((.((.......)).)))))))).........((((.....)))).............((((((((((((((((((((((((...((....))...)))))))))))))))))))))))).... (-47.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUACUUACACGUAAAGAAAUAUUUAUUUACACGUAGAAAAAUAUUUCUUUACGUGUAAAUAAUUUAUAGAAGUUUUGGUUUUACACCAUAAAAAAUACUUGAACAUACCCUAUUUGCCUAUCCAAGACGGAGAACCUAAUAAUGAACGAGAAGGUAGAUUUACCCAUUUUUAGGUA (((.(((.((((((((((((((((((((.((((....)))).)))))))))))))))))))).))).))).....(((((((...........................................))))))).....((((((.((((........((((....)))))))).)))))). (-41.93) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 500 160 113 104 57 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 485 170 121 181 132 pint:PI0115|5end_hypothetical_protein_108062..108292_POSITIVE 485 170 121 167 118 pint:PI0111|5end_hypothetical_protein_108048..108278_POSITIVE 485 170 121 132 83 pint:PI0112|5end_hypothetical_protein_108013..108243_POSITIVE 459 170 121 158 109 pint:PI0130|5end_hypothetical_protein_122032..122262_POSITIVE 457 170 121 148 99 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 446 170 121 136 87 pint:PI0648|5end_hypothetical_protein_589448..589678_POSITIVE 406 170 121 136 87 pint:PI0579|5end_hypothetical_protein_523419..523649_POSITIVE 400 176 131 138 93 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 399 170 121 158 109 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 394 160 113 217 170 pint:PI0702|5end_conserved_hypothetical_protein;_possible_tetracenomycin_C_synthesis_protein_homolog_638640..638870_POSITIVE 369 68 23 199 151 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 339 176 142 142 108 pint:PI1462|5end_hypothetical_protein_1385094..1385324_POSITIVE 339 176 142 215 181 pint:PI0173|5end_hypothetical_protein_165305..165535_POSITIVE 335 178 142 226 190 pint:PI0311|5end_hypothetical_protein_294721..294951_POSITIVE 318 59 24 98 64 pint:PI0688|5end_hypothetical_protein_625458..625688_POSITIVE 305 150 110 229 189 pint:PI0599|5end_hypothetical_protein_547376..547606_POSITIVE 302 150 107 116 72 pint:PI0535|5end_hypothetical_protein_489508..489738_POSITIVE 272 52 11 110 73 pint:PI1825|5end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_1764996..1765226_POSITIVE 268 143 107 231 194 pint:PI0114|5end_hypothetical_protein_108139..108369_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 440 176 132 54 9 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 428 160 111 60 11 pint:PI1314|3end_hypothetical_protein_1231423..1231573_POSITIVE 409 170 123 117 68 pint:PI2105|3end_hypothetical_protein_2059586..2059736_POSITIVE 339 176 142 41 7 pint:PI1461|3end_conserved_hypothetical_protein_1385073..1385223_POSITIVE 339 176 142 68 34 pint:PI0172|3end_ISPg3-related_transposase_165238..165388_POSITIVE 326 172 142 31 1 pint:PI2106|3end_hypothetical_protein_2059498..2059648_POSITIVE 295 176 131 47 2 pint:PI2160|3end_hypothetical_protein_2108357..2108507_POSITIVE 290 60 12 147 110 pint:PI0713|3end_hypothetical_protein_650529..650679_POSITIVE 284 169 121 54 11 pint:PI0808|3end_ISPg3-related_transposase_746457..746607_POSITIVE 278 178 142 85 49 pint:PI0725|3end_hypothetical_protein_658933..659083_POSITIVE 272 52 11 39 2 pint:PI1824|3end_ThiJ/PfpI_family_protein_1765005..1765155_POSITIVE 266 179 142 53 16 pint:PI0797|3end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735752..735902_POSITIVE 259 79 31 123 64 pint:PI0541|3end_GTP-binding_protein,_GTP1/OBG_family_495009..495159_POSITIVE 256 78 33 86 39 pint:PI1790|3end_hypothetical_protein_1722762..1722912_POSITIVE 255 170 127 44 1 pint:PI0701|3end_hypothetical_protein_638537..638687_POSITIVE 252 70 23 83 40 pint:PI1531|3end_biotin--acetyl-CoA-carboxylase_ligase_1451734..1451884_POSITIVE 252 170 123 66 17 pint:PI0533|3end_ABC_transporter,_permease_protein_488421..488571_POSITIVE 249 76 41 126 85 pint:PI1649|3end_hypothetical_protein_1563441..1563591_POSITIVE 241 50 2 60 18 pint:PI1807|3end_hypothetical_protein_1742481..1742631_POSITIVE 241 94 51 63 9 pint:PI1294|3end_hypothetical_protein_1208716..1208866_POSITIVE