Origin IGS: aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcgaaaccaccaaatggtgaaatcaatgcccctaatgctacatgcttgtcataaaggcatgttcttttattctccataattgctttagaattttattttttgtgctgccctttgatagttcctatcattgattcagctactgtttgatttaggttgggatacctcccaattttcttgcgatgcaaatataataaagttttttggaaaacacaaaaaaaatcagaagaattaatatcacattttcttcattgtttatttaaggtgcattctt Mask Tandem Repeat Region ================================================ aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Find is-nt database================================================ Query_seq: PI0970:PI0971|PI0970:PI0971:hypothetical protein:glycine cleavage system T protein (aminomethyltransferase):->->:897715..897986 272 aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0970:PI0971|PI0970:PI0971:hypothetical protein:glycine cleavage system T protein (aminomethyltransferase):->->:897715..897986 272 aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0970:PI0971|PI0970:PI0971:hypothetical protein:glycine cleavage system T protein (aminomethyltransferase):->->:897715..897986 272 aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 16 End: 120 ............... M N N E E N V I L I L L I F F V F S K K L Y Y I C I A R K L G G I P T ........................................................................................................................................................ Protein_Len: 46 Strand: + Start: 39 End: 176 ...................................... M N S S D F F C V F Q K T L L Y L H R K K I G R Y P N L N Q T V A E S M I G T I K G Q H K K ................................................................................................ Protein_Len: 34 Strand: - Start: 43 End: 144 .......................................... N K Q N K K H K G F F K I I N A D C S F Q S T D W G L D F L L Q I M ................................................................................................................................ Protein_Len: 39 Strand: - Start: 2 End: 118 . S H V K F L C H L F H S I L E E S K K Q T K W F V K N Y K C R L F I P L Y G M .......................................................................................................................................................... aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 83 HP_score: -7.6 Tail_Score: -4.65916 Start: 103 End: 118 Full_Region: actgtttgatttagg ttggga tacc tcccaa ttttcttgcgatgca ......................................................................................................ttgggatacctcccaa.......................................................................................................................................................... Find igs database================================================ Query_seq: PI0970:PI0971|PI0970:PI0971:hypothetical protein:glycine cleavage system T protein (aminomethyltransferase):->->:897715..897986 272 aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Intra-Species Hit: Count: 1 Min: 1 Max: 272 Len: 272 Subject: pint_PI0970_PI0971|hypothetical protein:glycine cleavage system T protein (aminomethyltransferase)|POSITIVE:POSITIVE|[897715, 897986]|272 HSP 1 e-value: 1.0E-138 bit: 492.0 Len: 272 Query Start:1 Query End:272 Subject Strand: POSITIVE Subject Start: 1 Subject End: 272 aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgannnnnnnngtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat aagaatgcaccttaaataaacaatgaagaaaatgtgatattaattcttctgattttttttgtgttttccaaaaaactttattatatttgcatcgcaagaaaattgggaggtatcccaacctaaatcaaacagtagctgaatcaatgataggaactatcaaagggcagcacaaaaaataaaattctaaagcaattatggagaataaaagaacatgcctttatgacaagcatgtagcattaggggcattgatttcaccatttggtggtttcgat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAAUGCACCUUAAAUAAACAAUGAAGAAAAUGUGAUAUUAAUUCUUCUGAUUUUUUUUGUGUUUUCCAAAAAACUUUAUUAUAUUUGCAUCGCAAGAAAAUUGGGAGGUAUCCCAACCUAAAUCAAACAGUAGCUGAAUCAAUGAUAGGAACUAUCAAAGGGCAGCACAAAAAAUAAAAUUCUAAAGCAAUUAUGGAGAAUAAAAGAACAUGCCUUUAUGACAAGCAUGUAGCAUUAGGGGCAUUGAUUUCACCAUUUGGUGGUUUCGAU ...(((((.((((((.......((((((...((((.......(((((((((((((((((((((((.(((...................((.((....))...((((((....))))))................))........((((((...))))))..))).)))))))))))..............))))).)))))))......))))..)))))).....((.....)).)))))))))))((..(((((....)))))..))... (-63.55) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCGAAACCACCAAAUGGUGAAAUCAAUGCCCCUAAUGCUACAUGCUUGUCAUAAAGGCAUGUUCUUUUAUUCUCCAUAAUUGCUUUAGAAUUUUAUUUUUUGUGCUGCCCUUUGAUAGUUCCUAUCAUUGAUUCAGCUACUGUUUGAUUUAGGUUGGGAUACCUCCCAAUUUUCUUGCGAUGCAAAUAUAAUAAAGUUUUUUGGAAAACACAAAAAAAAUCAGAAGAAUUAAUAUCACAUUUUCUUCAUUGUUUAUUUAAGGUGCAUUCUU ........((((....)))).....(((((.(((......((((((((.......))))))))..........((((..........(((((((((((.(((((((((..(..((((((...))))))..)...))))...(((..((...(((((((((....)))))))))))..)))..)))))...))))))))))).)))).................((((((.............))))))............))).)))))... (-59.22) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 316 270 221 110 70 pint:PI1547|5end_hypothetical_protein_1465808..1466038_POSITIVE 294 268 221 142 92 pint:PI0353|5end_hypothetical_protein_324610..324840_POSITIVE 287 269 222 203 154 pint:PI0371|5end_thymidylate_synthase_341258..341488_POSITIVE 273 248 206 73 6 pint:PI1781|5end_membrane-associated_zinc_metalloprotease_1713074..1713304_POSITIVE 272 172 135 118 75 pint:PI1468|5end_conserved_hypothetical_protein;_probable_TPR_domain_protein_1393900..1394130_POSITIVE 271 258 213 50 10 pint:PI0034|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_37151..37381_POSITIVE 264 263 221 52 13 pint:PI1937|5end_hypothetical_protein_1889411..1889641_POSITIVE 263 270 226 58 6 pint:PI1131|5end_folylpolyglutamate_synthase_1057124..1057354_POSITIVE 262 139 91 68 15 pint:PI1007|5end_conserved_hypothetical_protein_926956..927186_POSITIVE 262 260 212 210 166 pint:PI0764|5end_permease_697962..698192_POSITIVE 261 121 89 180 141 pint:PI0275|5end_hypothetical_protein_263138..263368_POSITIVE 260 270 223 130 86 pint:PI1159|5end_1,4-alpha-glucan_branching_enzyme,_N-terminal_region_1082836..1083066_POSITIVE 260 270 225 206 160 pint:PI0017|5end_thiol-disulfide_isomerase;_thioredoxin_21733..21963_POSITIVE 258 136 93 116 79 pint:PI0062|5end_endopeptidase_60963..61193_POSITIVE 255 270 221 229 184 pint:PI2155|5end_hypothetical_protein_2100664..2100894_POSITIVE 254 169 132 56 3 pint:PI0967|5end_conserved_hypothetical_protein_895424..895654_POSITIVE 252 255 213 50 10 pint:PI0628|5end_phosphoribosylglycinamide_formyltransferase_576027..576257_POSITIVE 252 244 212 139 106 pint:PI0213|5end_conserved_hypothetical_protein_207065..207295_POSITIVE 251 244 206 201 160 pint:PI0269|5end_conserved_hypothetical_protein_259835..260065_POSITIVE 247 69 43 170 140 pint:PI2140|5end_conserved_hypothetical_protein_2086773..2087003_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 272 172 135 47 4 pint:PI1467|3end_conserved_hypothetical_protein_1393909..1394059_POSITIVE 270 257 212 83 30 pint:PI0138|3end_hypothetical_protein_131365..131515_POSITIVE 264 263 221 49 10 pint:PI1936|3end_conserved_hypothetical_protein;_possible_serine-type_protease_1889488..1889638_POSITIVE 264 267 227 65 22 pint:PI1843|3end_hypothetical_protein_1788611..1788761_POSITIVE 262 139 91 81 28 pint:PI1006|3end_conserved_hypothetical_protein_927049..927199_POSITIVE 261 121 89 149 110 pint:PI0274|3end_conserved_hypothetical_protein_263187..263337_POSITIVE 260 270 223 124 80 pint:PI1158|3end_1,4-alpha-glucan_branching_enzyme,_C-terminal_region_1082910..1083060_POSITIVE 260 167 131 97 58 pint:PI0892|3end_acyl-ACP_thioesterase_823939..824089_POSITIVE 260 270 225 118 72 pint:PI0016|3end_magnesium_chelatase,_subunit_ChlI_21725..21875_POSITIVE 254 169 130 123 77 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 252 267 221 148 92 pint:PI1260|3end_IS1001-related_transposase_1183272..1183422_POSITIVE 251 244 206 76 35 pint:PI0268|3end_lysozyme-related_protein_259790..259940_POSITIVE 247 69 43 69 39 pint:PI2139|3end_cysteinyl-tRNA_synthetase_2086752..2086902_POSITIVE 245 169 137 68 32 pint:PI0375|3end_hypothetical_protein_346082..346232_POSITIVE 237 265 227 97 52 pint:PI1048|3end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_967193..967343_POSITIVE 236 270 226 85 36 pint:PI0566|3end_enoyl-[acyl-carrier-protein]_reductase_512628..512778_POSITIVE 232 260 230 32 4 pint:PI1820|3end_hypothetical_protein_1760377..1760527_POSITIVE 232 268 225 129 75 pint:PI1730|3end_hypothetical_protein_1650367..1650517_POSITIVE 232 228 186 116 82 pint:PI0779|3end_predicted_TPR-repeat-containing_protein_715800..715950_POSITIVE 231 265 226 126 70 pint:PI1789|3end_hypothetical_protein_1719037..1719187_POSITIVE