Origin IGS: taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacctcctcgtcatcattgtcaaacacatctacgatatcgcttttcgttgtgctgccacttacaggttcagagtggtggcaattcgggcaaacaacgtcttccttcagatagatttcgctacaatggaaacactttacgaggtgccccgcaatttctactccacaacttggacaaactggtgctttatcgctgacttgatgtccacattcaggacactttataatcattgctattgaattttaattttacagtatcgttagtaagtcaatgtgtatgggctacttccttattta Mask Tandem Repeat Region ================================================ taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Find is-nt database================================================ Query_seq: PI0966:PI0967|PI0966:PI0967:possible glycosyl hydrolase:conserved hypothetical protein:->->:895270..895563 294 taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0966:PI0967|PI0966:PI0967:possible glycosyl hydrolase:conserved hypothetical protein:->->:895270..895563 294 taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0966:PI0967|PI0966:PI0967:possible glycosyl hydrolase:conserved hypothetical protein:->->:895270..895563 294 taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 61 End: 294 ............................................................ M A M I I K C P E C G H Q V S D K A P V C P S C G V E I A G H L V K C F H C S E I Y L K E D V V C P N C H H S E P V S G Protein_Len: 31 Strand: - Start: 186 End: 278 ......................................................................................................................................................................................... R F S S T T Q G F Q W W E S G T L P L V V F L S I T S T N S M ................ Protein_Len: 48 Strand: - Start: 104 End: 247 ....................................................................................................... R Y L V L K D L N H L L F Q P A G R L T N G N Y R F R D S P L R Q K G S N G G S Q V Q L H C C M ............................................... Protein_Len: 60 Strand: - Start: 61 End: 282 ............................................................ A I F C W N T W T T S Y F N R P V E Y L T E M T A F D I Q L F V N N A R I A V V R F R Y T A A C R F A I D Y I H K V I M ............ taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0966:PI0967|PI0966:PI0967:possible glycosyl hydrolase:conserved hypothetical protein:->->:895270..895563 294 taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Intra-Species Hit: Count: 1 Min: 1 Max: 294 Len: 294 Subject: pint_PI0966_PI0967|possible glycosyl hydrolase:conserved hypothetical protein|POSITIVE:POSITIVE|[895270,895563]|294 HSP 1 e-value: 1.0E-165 bit: 583.0 Len: 294 Query Start:1 Query End:294 Subject Strand: POSITIVE Subject Start: 1 Subject End: 294 taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta taaataaggaagtagcccatacacattgacttactaacgatactgtaaaattaaaattcaatagcaatgattataaagtgtcctgaatgtggacatcaagtcagcgataaagcaccagtttgtccaagttgtggagtagaaattgcggggcacctcgtaaagtgtttccattgtagcgaaatctatctgaaggaagacgttgtttgcccgaattgccaccactctgaacctgtaagtggcagcacaacgaaaagcgatatcgtagatgtgtttgacaatgatgacgaggaggta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAAGGAAGUAGCCCAUACACAUUGACUUACUAACGAUACUGUAAAAUUAAAAUUCAAUAGCAAUGAUUAUAAAGUGUCCUGAAUGUGGACAUCAAGUCAGCGAUAAAGCACCAGUUUGUCCAAGUUGUGGAGUAGAAAUUGCGGGGCACCUCGUAAAGUGUUUCCAUUGUAGCGAAAUCUAUCUGAAGGAAGACGUUGUUUGCCCGAAUUGCCACCACUCUGAACCUGUAAGUGGCAGCACAACGAAAAGCGAUAUCGUAGAUGUGUUUGACAAUGAUGACGAGGAGGUA ..............((((.....(.(((((((..........((((..(((....)))..)))).............((((((.......)))))).))))))).).....(((....((((((((.....)))).))))...))).)))).((((((....((((.(((((...((((.(((..(((......)))((((((..((......((((((.((.........))..))))))))))))))......))).)))).)))).)...))))......))))))..... (-65.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACCUCCUCGUCAUCAUUGUCAAACACAUCUACGAUAUCGCUUUUCGUUGUGCUGCCACUUACAGGUUCAGAGUGGUGGCAAUUCGGGCAAACAACGUCUUCCUUCAGAUAGAUUUCGCUACAAUGGAAACACUUUACGAGGUGCCCCGCAAUUUCUACUCCACAACUUGGACAAACUGGUGCUUUAUCGCUGACUUGAUGUCCACAUUCAGGACACUUUAUAAUCAUUGCUAUUGAAUUUUAAUUUUACAGUAUCGUUAGUAAGUCAAUGUGUAUGGGCUACUUCCUUAUUUA .....((((((.....................(((.(((....((((...(((..((((((.........))))))..)))...))))........((((......)))).))).)))......((....))....)))))).((((.(((........((((.....))))........))).....((((((((((.(((((.......))))).....((((...((((...((((....))))...))))..)))).)))))))..)))...)))).............. (-63.69) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 425 250 206 67 11 pint:PI0536|5end_hypothetical_protein_492163..492393_POSITIVE 340 275 231 80 23 pint:PI1345|5end_conserved_hypothetical_protein;_possible_TPR-repeat-containing_protein_1259185..1259415_POSITIVE 340 259 212 56 14 pint:PI0488|5end_conserved_hypothetical_protein_446906..447136_POSITIVE 334 270 223 179 131 pint:PI2034|5end_conserved_hypothetical_protein_1987573..1987803_POSITIVE 331 180 132 220 176 pint:PI1675|5end_TonB-dependent_outer_membrane_receptor_1588107..1588337_POSITIVE 328 158 111 225 164 pint:PI1002|5end_metal_dependent_hydrolase_923842..924072_POSITIVE 325 290 241 100 52 pint:PI0792|5end_conserved_hypothetical_protein_729068..729298_POSITIVE 324 150 101 62 10 pint:PI1816|5end_acid_phosphatase_1754646..1754876_POSITIVE 318 239 191 85 36 pint:PI0021|5end_orotate_phosphoribosyltransferase_24939..25169_POSITIVE 317 249 204 53 8 pint:PI0894|5end_2-oxoglutarate_oxidoreductase,_gamma_subunit_(2-oxoglutarate-ferredoxin_oxidoreductase)_825776..826006_POSITIVE 312 257 214 55 10 pint:PI2159|5end_immunoreactive_84_kDa_antigen_PG93_2105267..2105497_POSITIVE 312 147 104 146 103 pint:PI0758|5end_hypothetical_protein_690247..690477_POSITIVE 311 138 100 93 53 pint:PI0519|5end_5'-nucleotidase/2',3'-cyclic_phosphodiesterase_477953..478183_POSITIVE 309 137 91 202 134 pint:PI1460|5end_ribonucleotide_reductase,_alpha_subunit,_N-terminal_region_1382215..1382445_POSITIVE 309 126 82 229 168 pint:PI0937|5end_hypothetical_protein_869235..869465_POSITIVE 305 150 102 106 49 pint:PI1923|5end_conserved_hypothetical_protein_1865560..1865790_POSITIVE 305 269 221 93 45 pint:PI1550|5end_conserved_hypothetical_protein_1467360..1467590_POSITIVE 303 158 111 216 167 pint:PI1279|5end_RNA_polymerase_sigma_factor/ECF_subfamily_(sigma-70)_1195215..1195445_POSITIVE 303 240 198 81 37 pint:PI0932|5end_conserved_hypothetical_protein;_possible_hemin_receptor_863854..864084_POSITIVE 302 256 214 175 122 pint:PI0581|5end_conserved_hypothetical_protein_525055..525285_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 392 220 178 47 9 pint:PI0670|3end_hypothetical_protein_611280..611430_POSITIVE 375 238 193 140 97 pint:PI1882|3end_hypothetical_protein_1826487..1826637_POSITIVE 325 290 241 61 13 pint:PI0791|3end_conserved_hypothetical_protein_729109..729259_POSITIVE 312 257 214 55 10 pint:PI2158|3end_glycosyl_transferase,_group_2_family_protein_2105347..2105497_POSITIVE 310 289 250 151 114 pint:PI2150|3end_acyl_carrier_protein_2097285..2097435_POSITIVE 300 170 126 106 53 pint:PI1414|3end_conserved_hypothetical_protein_1334436..1334586_POSITIVE 298 180 133 101 54 pint:PI0592|3end_DNA_mismatch_repair_protein_542611..542761_POSITIVE 296 158 118 62 15 pint:PI1458|3end_dihydroneopterin_aldolase_1381626..1381776_POSITIVE 295 239 198 148 102 pint:PI0203|3end_conserved_hypothetical_protein_196064..196214_POSITIVE 293 250 207 135 86 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE 291 178 131 139 91 pint:PI1945|3end_ribonuclease_HII_1902587..1902737_POSITIVE 290 267 222 74 24 pint:PI0007|3end_hypothetical_protein_7300..7450_POSITIVE 289 159 111 141 86 pint:PI1437|3end_hypothetical_protein_1356407..1356557_POSITIVE 289 258 214 62 20 pint:PI0308|3end_conserved_hypothetical_protein_291602..291752_POSITIVE 287 160 111 121 70 pint:PI0583|3end_hypothetical_protein_526276..526426_POSITIVE 286 270 222 93 23 pint:PI0844|3end_cytidine/deoxycytidylate_deaminase_(dCMP_deaminase)_778316..778466_POSITIVE 284 280 232 130 86 pint:PI1736|3end_topoisomerase_IV,_subunit_A_1657090..1657240_POSITIVE 283 179 131 73 27 pint:PI1827|3end_peptidase,_M23/M37_family,_N-terminal_1768602..1768752_POSITIVE 283 247 201 57 8 pint:PI0053|3end_hypothetical_protein_55124..55274_POSITIVE 282 149 102 141 91 pint:PI2041|3end_conserved_hypothetical_protein_1994660..1994810_POSITIVE