Origin IGS: ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaagaaacgcataaggcatctttgaaatgccattaacaaagagaaagtcctgaccgattacaagtttggtcagggctttttaaagttaataccatatggaagttaatcgttgaatgaaagcaaattacagaagtcaatctttaaacaacacatataagattttttaatgcttacaaaattgattattcggaataattttgtatatttgcagatactacaaggtattcatttaaaatagaaaacaagaa Mask Tandem Repeat Region ================================================ ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Find is-nt database================================================ Query_seq: PI0946:PI0947|PI0946:PI0947:glutathione peroxidase:conserved hypothetical protein:->->:878665..878915 251 ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0946:PI0947|PI0946:PI0947:glutathione peroxidase:conserved hypothetical protein:->->:878665..878915 251 ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0946:PI0947|PI0946:PI0947:glutathione peroxidase:conserved hypothetical protein:->->:878665..878915 251 ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 24 End: 143 ....................... M P C S I C K Y T K L F R I I N F V S I K K S Y M C C L K I D F C N L L S F N D ............................................................................................................ Protein_Len: 34 Strand: + Start: 112 End: 213 ............................................................................................................... M T S V I C F H S T I N F H M V L T L K S P D Q T C N R S G L S L C ...................................... ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 62 HP_score: -3.5 Tail_Score: -4.46976 Start: 173 End: 202 Full_Region: TATTAACTTTAAAAA GCCCTGACCAA ACTTGTAA TCGGTCAGGAC TTTCTCTTTGTTAAT ............................................................................................................................................................................GCCCTGACCAAACTTGTAATCGGTCAGGAC................................................. TransTerm Strand: - Conf: 83 HP_score: -7.9 Tail_Score: -4.67904 Start: 173 End: 202 Full_Region: attaacaaagagaaa gtcctgaccga ttacaagt ttggtcagggc tttttaaagttaata ............................................................................................................................................................................gtcctgaccgattacaagtttggtcagggc................................................. TransTerm Strand: + Conf: 50 HP_score: -4.7 Tail_Score: -2.54851 Start: 175 End: 200 Full_Region: TTAACTTTAAAAAGC CCTGACCAA ACTTGTAA TCGGTCAGG ACTTTCTCTTTGTTA ..............................................................................................................................................................................CCTGACCAAACTTGTAATCGGTCAGG................................................... Find igs database================================================ Query_seq: PI0946:PI0947|PI0946:PI0947:glutathione peroxidase:conserved hypothetical protein:->->:878665..878915 251 ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Intra-Species Hit: Count: 1 Min: 1 Max: 251 Len: 251 Subject: pint_PI0946_PI0947|glutathione peroxidase:conserved hypothetical protein|POSITIVE:POSITIVE|[878665,878915]|251 HSP 1 e-value: 1.0E-140 bit: 498.0 Len: 251 Query Start:1 Query End:251 Subject Strand: POSITIVE Subject Start: 1 Subject End: 251 ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta ttcttgttttctattttaaatgaataccttgtagtatctgcaaatatacaaaattattccgaataatcaattttgtaagcattaaaaaatcttatatgtgttgtttaaagattgacttctgtaatttgctttcattcaacgattaacttccatatggtattaactttaaaaagccctgaccaaacttgtaatcggtcaggactttctctttgttaatggcatttcaaagatgccttatgcgtttcttttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUUGUUUUCUAUUUUAAAUGAAUACCUUGUAGUAUCUGCAAAUAUACAAAAUUAUUCCGAAUAAUCAAUUUUGUAAGCAUUAAAAAAUCUUAUAUGUGUUGUUUAAAGAUUGACUUCUGUAAUUUGCUUUCAUUCAACGAUUAACUUCCAUAUGGUAUUAACUUUAAAAAGCCCUGACCAAACUUGUAAUCGGUCAGGACUUUCUCUUUGUUAAUGGCAUUUCAAAGAUGCCUUAUGCGUUUCUUUUUA .............(((((((..((((((.(((.......((((((.((((.(((((.((.((((((.((....((((((.(((....)))))))))..))))))))...)).))).)).))))))))))...........((......)).))).))))))...)))))))...(((((((............)))))))...........((....((((((.....))))))....))........... (-43.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAGAAACGCAUAAGGCAUCUUUGAAAUGCCAUUAACAAAGAGAAAGUCCUGACCGAUUACAAGUUUGGUCAGGGCUUUUUAAAGUUAAUACCAUAUGGAAGUUAAUCGUUGAAUGAAAGCAAAUUACAGAAGUCAAUCUUUAAACAACACAUAUAAGAUUUUUUAAUGCUUACAAAAUUGAUUAUUCGGAAUAAUUUUGUAUAUUUGCAGAUACUACAAGGUAUUCAUUUAAAAUAGAAAACAAGAA ........(((.((((.(((((.......))))).(((((....((((((((((((((((........))))))))))))))))...))))).....))))...)))..((.((((((((..(((((((((((((...((((((..............)))))).................((((....)))).....))))))).))))))..(((((....)))))))))))))....))......... (-56.44) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 299 250 207 185 142 pint:PI1768|5end_conserved_hypothetical_protein_1695456..1695686_POSITIVE 287 245 203 152 107 pint:PI1705|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_III_1621781..1622011_POSITIVE 280 240 191 105 44 pint:PI1078|5end_conserved_hypothetical_protein_999500..999730_POSITIVE 280 250 204 198 155 pint:PI0085|5end_RNase_P_protein_component_84440..84670_POSITIVE 271 240 192 86 30 pint:PI2175|5end_RNA_polymerase_sigma-54_2115007..2115237_POSITIVE 270 240 192 57 7 pint:PI1711|5end_ABC_transporter,_ATP-binding_protein_1627597..1627827_POSITIVE 262 244 208 85 46 pint:PI0839|5end_conserved_hypothetical_protein_773843..774073_POSITIVE 262 220 171 165 111 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 261 240 193 141 102 pint:PI0301|5end_probable_transposase_285672..285902_POSITIVE 259 212 176 200 157 pint:PI2074|5end_conserved_hypothetical_protein_2033660..2033890_POSITIVE 259 240 191 216 163 pint:PI1931|5end_alpha_glycan_phosphorylase,_maltodextrin_phosphorylase_1875456..1875686_POSITIVE 255 232 194 215 173 pint:PI0243|5end_hypothetical_protein_240506..240736_POSITIVE 254 219 172 78 36 pint:PI0257|5end_conjugative_transposon_protein,_TraF_249348..249578_POSITIVE 253 240 195 70 37 pint:PI0960|5end_hypothetical_protein_891137..891367_POSITIVE 252 210 163 214 173 pint:PI2176|5end_conserved_hypothetical_protein_2116484..2116714_POSITIVE 249 156 113 167 114 pint:PI0181|5end_conserved_hypothetical_protein_173435..173665_POSITIVE 248 239 192 85 28 pint:PI1164|5end_hypothetical_protein_1088203..1088433_POSITIVE 247 240 194 161 110 pint:PI0601|5end_hypothetical_protein_548758..548988_POSITIVE 246 218 172 149 94 pint:PI0383|5end_conserved_hypothetical_protein;_possible_Zn-dependent_hydrolase_352153..352383_POSITIVE 245 208 162 222 169 pint:PI1636|5end_hypothetical_protein_1554246..1554476_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 280 240 191 94 33 pint:PI1077|3end_hypothetical_protein_999569..999719_POSITIVE 274 208 170 100 62 pint:PI0745|3end_conserved_hypothetical_protein_675670..675820_POSITIVE 269 220 172 126 71 pint:PI0997|3end_hypothetical_protein_922293..922443_POSITIVE 262 244 208 89 50 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE 261 240 192 147 94 pint:PI1082|3end_polypeptide_deformylase_1001922..1002072_POSITIVE 261 240 193 150 111 pint:PI0300|3end_transposase_285761..285911_POSITIVE 254 219 172 70 28 pint:PI0256|3end_conjugative_transposon_protein,_TraE_249420..249570_POSITIVE 253 240 195 57 24 pint:PI0959|3end_uridine_kinase_891204..891354_POSITIVE 249 156 113 82 29 pint:PI0180|3end_TonB_protein_173430..173580_POSITIVE 247 240 194 120 69 pint:PI0600|3end_bifunctional_purine_biosynthesis_protein,_5-aminoimidazole-4-carboxamide_ribonucleotide(AICAR)_transformylase/IMP_cyclohydrolase_548797..548947_POSITIVE 241 162 128 90 57 pint:PI0865|3end_possible_phosphoglycerol_transferase_803684..803834_POSITIVE 241 220 172 132 90 pint:PI0482|3end_50S_ribosomal_protein_L36_443539..443689_POSITIVE 240 220 172 83 30 pint:PI0052|3end_hypothetical_protein_52715..52865_POSITIVE 238 62 25 96 53 pint:PI0796|3end_competence_protein_734954..735104_POSITIVE 231 220 172 50 7 pint:PI0065|3end_conserved_hypothetical_protein_65312..65462_POSITIVE 226 210 163 140 103 pint:PI0359|3end_hypothetical_protein_328835..328985_POSITIVE 225 240 194 98 67 pint:PI2145|3end_hypothetical_protein_2092649..2092799_POSITIVE 225 241 201 95 61 pint:PI0595|3end_conserved_hypothetical_protein_546406..546556_POSITIVE 224 200 154 65 15 pint:PI1734|3end_possible_carboxyl-terminal_protease-related_protein_1653829..1653979_POSITIVE 223 246 204 89 50 pint:PI1745|3end_conserved_hypothetical_protein_1667417..1667567_POSITIVE