Origin IGS: acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgcttgcagcaacattgttttcgttgcctgcagcagcacaggaaacatacgagaacgctcgtttgacgggcaacgaccttaacggtacagcccgctatgtaggtatgggtggagcaatggaagccttaggagctgatatttctaccataggaagcaaccctgcaggtgtcggt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 accgacacctgcagggttgcttcctatggtagaaatatcagctcctaaggcttccattgctccacccatacctacatagcgggctgtaccgttaaggtcgttgcccgtcaaacgagcgttctcgtatgtttcctgtgctgctgcaggcaacgaaaacaatgttgctgcaagcaaataatgatgtattttcataattatatttctgtttttgacctgttaaactaacttttctattataaaaagcaagatttattatctacgaccaccgaagccgccgccacccgaatgtgaaccgccaccactgcgtgagccaccgaaactgccaccgcctaccgagtgtgaaggacctcttgatgggctcgggttgtagttacggggtgtttcacgttggttgccgaacgagtttctgttatcaaatacttctcttcttggcgatatttcagactttctgtcgcgagaaagcgtgccaaaactgttgcctctgttgctagaagagttattcctttctatcacaactcggtttcttgagttgccaaactgaccgctgcgtgcgttcatattgccgaacgaacgtggagaatcgacattacgaggttgtccgttatagacagaggttctgcttccaaagctgccgccgtatgcaccaccccagtggttagccgtacccgagtgtcctccagcgtagttgtatctgatgtaaggtcttgcgtaaccgtaatagtagtaagggtatccccaaccgtaatagccgtacccccaagggtcataccagccacggcggtaacggtaataccaaggatcgtaatatcccatataccacggatcgtagtaaccccagtaccaagggtcccaacttctgt Mask Tandem Repeat Region ================================================ acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgcttgcagcaacattgttttcgttgcctgcagcagcacaggaaacatacgagaacgctcgtttgacgggcaacgaccttaacggtacagcccgctatgtaggtatgggtggagcaatggaagccttaggagctgatatttctaccataggaagcaaccctgcaggtgtcggt Find is-nt database================================================ Query_seq: PI0931:PI0932|PI0931:PI0932:hypothetical protein:conserved hypothetical protein; possible hemin receptor:->->:863134..863993 860 acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgcttgcagcaacattgttttcgttgcctgcagcagcacaggaaacatacgagaacgctcgtttgacgggcaacgaccttaacggtacagcccgctatgtaggtatgggtggagcaatggaagccttaggagctgatatttctaccataggaagcaaccctgcaggtgtcggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0931:PI0932|PI0931:PI0932:hypothetical protein:conserved hypothetical protein; possible hemin receptor:->->:863134..863993 860 acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgcttgcagcaacattgttttcgttgcctgcagcagcacaggaaacatacgagaacgctcgtttgacgggcaacgaccttaacggtacagcccgctatgtaggtatgggtggagcaatggaagccttaggagctgatatttctaccataggaagcaaccctgcaggtgtcggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0931:PI0932|PI0931:PI0932:hypothetical protein:conserved hypothetical protein; possible hemin receptor:->->:863134..863993 860 acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgcttgcagcaacattgttttcgttgcctgcagcagcacaggaaacatacgagaacgctcgtttgacgggcaacgaccttaacggtacagcccgctatgtaggtatgggtggagcaatggaagccttaggagctgatatttctaccataggaagcaaccctgcaggtgtcggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 690 Max: 860 Len: 171 Subject: UniRef90_Q5LEV6 Cluster: Conserved hypothetical exported protein; n=1; Bacteroides fragilis NCTC 9343|Rep: Conserved hypothetical exported protein - Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) HSP 1 e-value: 3.0E-13 bit: 78.2 Len: 171 Query Start:690 Query End:860 Subject Strand: null Subject Start: 10 Subject End: 66 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L A A T L F S L P A A A Q E T Y E N A R L T G N D L N G T A R Y V G M G G A M E A L G A D I S T I G S N P A G V G ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L G L L V A A A P I S A Q T V Y D A A K I T G K D L N G T A R F V G M G G A M G A L G G D I S T I G T N P A G I G Subject: UniRef90_Q8AAZ6 Cluster: Putative hemin receptor; n=1; Bacteroides thetaiotaomicron|Rep: Putative hemin receptor - Bacteroides thetaiotaomicron HSP 1 e-value: 1.0E-11 bit: 72.8 Len: 165 Query Start:696 Query End:860 Subject Strand: null Subject Start: 11 Subject End: 65 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... A T L F S L P A A A Q E T Y E N A R L T G N D L N G T A R Y V G M G G A M E A L G A D I S T I G S N P A G V G ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... A L I M V A S A S A Q T I Y D G A K F T Q K D L N G T A R F V G M G G A M G A L G G D I S T M G T N P A G I G Subject: UniRef90_Q64VU5 Cluster: Putative hemin receptor; n=1; Bacteroides fragilis|Rep: Putative hemin receptor - Bacteroides fragilis HSP 1 e-value: 2.0E-11 bit: 72.4 Len: 159 Query Start:702 Query End:860 Subject Strand: null Subject Start: 13 Subject End: 65 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L F S L P A A A Q E T Y E N A R L T G N D L N G T A R Y V G M G G A M E A L G A D I S T I G S N P A G V G ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L W T A G A N A Q T A Y D A A K L V S K D L N G T A R F V G M G G A M G A L G G D I S T M G T N P A G I G acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 101 End: 202 .................................................................................................... M G V R L L R L G I P L L L L R L R K T L H Q I Q L R W R T L G Y G .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 37 End: 303 .................................... M R G I W D I T I L G I T V T A V A G M T L G G T A I T V G D T L T T I T V T Q D L T S D T T T L E D T R V R L T T G V ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 56 Strand: + Start: 361 End: 528 ........................................................................................................................................................................................................................................................................................................................................................................ M T L L A T E A T V L A R F L A T E S L K Y R Q E E K Y L I T E T R S A T N V K H P V T T T R A H Q E V L H T R ............................................................................................................................................................................................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 506 End: 601 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K R S F T L G R R W Q F R W L T Q W W R F T F G W R R L R W S ................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 48 End: 605 ............................................... M G Y Y D P W Y Y R Y R R G W Y D P W G Y G Y Y G W G Y P Y Y Y Y G Y A R P Y I R Y N Y A G G H S G T A N H W G G A Y G ............................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 535 End: 624 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M A V S V A H A V V A V H I R V A A A S V V V D N K S C F L ............................................................................................................................................................................................................................................ Protein_Len: 54 Strand: + Start: 605 End: 766 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M I N L A F Y N R K V S L T G Q K Q K Y N Y E N T S L F A C S N I V F V A C S S T G N I R E R S F D G Q R P .............................................................................................. Protein_Len: 60 Strand: + Start: 663 End: 860 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M I M K I H H Y L L A A T L F S L P A A A Q E T Y E N A R L T G N D L N G T A R Y V G M G G A M E A L G A D I S T I G S Protein_Len: 49 Strand: - Start: 639 End: 785 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. N L L D F V S I Y N H F Y M M I Q K C C C Q K R Q R C C C L F C V L V S T Q R A V V K V T C G A M ........................................................................... Protein_Len: 45 Strand: - Start: 494 End: 628 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L G L G D L P G E C E T P P P L K P P E R L P P P E C E P P P P K P P R L Y Y I K S K I M ........................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 373 End: 609 .................................................................................................................................................................................................................................................................................................................................................................................... L F L L I Q Y C F S T R C G V H F V G Y S C G S G M L L D K V S P L R H C N R H S V C H H R N V N P H R R S R H D Y I M ........................................................................................................................................................................................................................................................... Protein_Len: 46 Strand: - Start: 336 End: 473 ............................................................................................................................................................................................................................................................................................................................................... S V S N H Y F P I V R R A V S A V T K A R K R A V S L R F Y R W S S F Y K I V S V R E A V M ................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 49 Strand: - Start: 323 End: 469 .................................................................................................................................................................................................................................................................................................................................. N P L E L F R T T I S L F L E E L L L P L L K P V S E R S L F D S I D G L L S T N S L L F E N P M ....................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: - Start: 205 End: 336 ............................................................................................................................................................................................................ G S P H H M R R C S Q F C F R Q R Y R V V E Y H R N E V N T R C Y S R V C R D T Q C S M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 3 End: 323 .. V T V C S R V D S V V V S S S V R T R S V V P T T C V A A A K S A S G R D I V S L R T I D I R W T R E A I H V C A A T M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 1 End: 156 C F N P G K T S P N S R D T T Y P I N R D K T N G N G G H S T H G K P T R S N R N P I G K S S N R N R M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 624 End: 653 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGACCTGTTAAACTAACTTTTCTATTAT............................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 71 HP_score: -4.0 Tail_Score: -4.73682 Start: 635 End: 645 Full_Region: ttctgtttttgacct gtta aac taac ttttctattataaaa ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gttaaactaac....................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PI0931:PI0932|PI0931:PI0932:hypothetical protein:conserved hypothetical protein; possible hemin receptor:->->:863134..863993 860 acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattatttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Intra-Species Hit: Count: 1 Min: 1 Max: 688 Len: 688 Subject: pint_PI0931_PI0932|hypothetical protein:conserved hypothetical protein; possible hemin receptor|POSITIVE:POSITIVE|[863134,863993]|860 HSP 1 e-value: 0.0 bit: 1364.0 Len: 688 Query Start:1 Query End:688 Subject Strand: POSITIVE Subject Start: 1 Subject End: 688 acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattattt............................................................................................................................................................................ acagaagttgggacccttggtactggggttactacgatccgtggtatatgggatattacgatccttggtattaccgttaccgccgtggctggtatgacccttgggggtacggctattacggttggggatacccttactactattacggttacgcaagaccttacatcagatacaactacgctggaggacactcgggtacggctaaccactggggtggtgcatacggcggcagctttggaagcagaacctctgtctataacggacaacctcgtaatgtcgattctccacgttcgttcggcaatatgaacgcacgcagcggtcagtttggcaactcaagaaaccgagttgtgatagaaaggaataactcttctagcaacagaggcaacagttttggcacgctttctcgcgacagaaagtctgaaatatcgccaagaagagaagtatttgataacagaaactcgttcggcaaccaacgtgaaacaccccgtaactacaacccgagcccatcaagaggtccttcacactcggtaggcggtggcagtttcggtggctcacgcagtggtggcggttcacattcgggtggcggcggcttcggtggtcgtagataataaatcttgctttttataatagaaaagttagtttaacaggtcaaaaacagaaatataattatgaaaatacatcattattt............................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAGAAGUUGGGACCCUUGGUACUGGGGUUACUACGAUCCGUGGUAUAUGGGAUAUUACGAUCCUUGGUAUUACCGUUACCGCCGUGGCUGGUAUGACCCUUGGGGGUACGGCUAUUACGGUUGGGGAUACCCUUACUACUAUUACGGUUACGCAAGACCUUACAUCAGAUACAACUACGCUGGAGGACACUCGGGUACGGCUAACCACUGGGGUGGUGCAUACGGCGGCAGCUUUGGAAGCAGAACCUCUGUCUAUAACGGACAACCUCGUAAUGUCGAUUCUCCACGUUCGUUCGGCAAUAUGAACGCACGCAGCGGUCAGUUUGGCAACUCAAGAAACCGAGUUGUGAUAGAAAGGAAUAACUCUUCUAGCAACAGAGGCAACAGUUUUGGCACGCUUUCUCGCGACAGAAAGUCUGAAAUAUCGCCAAGAAGAGAAGUAUUUGAUAACAGAAACUCGUUCGGCAACCAACGUGAAACACCCCGUAACUACAACCCGAGCCCAUCAAGAGGUCCUUCACACUCGGUAGGCGGUGGCAGUUUCGGUGGCUCACGCAGUGGUGGCGGUUCACAUUCGGGUGGCGGCGGCUUCGGUGGUCGUAGAUAAUAAAUCUUGCUUUUUAUAAUAGAAAAGUUAGUUUAACAGGUCAAAAACAGAAAUAUAAUUAUGAAAAUACAUCAUUAUUU ...(((....(((((.(((.(((((((((((((.......(((((((..(((((......)))))..))))))).(((((((((...((((((..(((((((((((((.(((.......(((.(((....)))..)))........(((((((...........(((((((((.....((((((((((...((((.(((((.........(((((..(((.((.(((....))).))...)))..))))).((((.....))))....)))))...)))))))))).(((.((((((......)))))).))).)))).((..(((.(((((((........)))))))....)))..))....(((((((.((......(....).((((..(...(((((((......))))))))..))))...))..)))))))..)))))))))........(.((((.(....).)))).)........))))))).....))).)))).))))).))))......)).)))).)))))))))......))))))))...))))).))).)))))...))).(((((((((.((....)).))))).(((..((((.((.(((((((......))))))).))))))....)))...........................))))....... (-204.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAAUGAUGUAUUUUCAUAAUUAUAUUUCUGUUUUUGACCUGUUAAACUAACUUUUCUAUUAUAAAAAGCAAGAUUUAUUAUCUACGACCACCGAAGCCGCCGCCACCCGAAUGUGAACCGCCACCACUGCGUGAGCCACCGAAACUGCCACCGCCUACCGAGUGUGAAGGACCUCUUGAUGGGCUCGGGUUGUAGUUACGGGGUGUUUCACGUUGGUUGCCGAACGAGUUUCUGUUAUCAAAUACUUCUCUUCUUGGCGAUAUUUCAGACUUUCUGUCGCGAGAAAGCGUGCCAAAACUGUUGCCUCUGUUGCUAGAAGAGUUAUUCCUUUCUAUCACAACUCGGUUUCUUGAGUUGCCAAACUGACCGCUGCGUGCGUUCAUAUUGCCGAACGAACGUGGAGAAUCGACAUUACGAGGUUGUCCGUUAUAGACAGAGGUUCUGCUUCCAAAGCUGCCGCCGUAUGCACCACCCCAGUGGUUAGCCGUACCCGAGUGUCCUCCAGCGUAGUUGUAUCUGAUGUAAGGUCUUGCGUAACCGUAAUAGUAGUAAGGGUAUCCCCAACCGUAAUAGCCGUACCCCCAAGGGUCAUACCAGCCACGGCGGUAACGGUAAUACCAAGGAUCGUAAUAUCCCAUAUACCACGGAUCGUAGUAACCCCAGUACCAAGGGUCCCAACUUCUGU (((((..((((((.((....)).))))))..)))))..(((((.....(((.(((...(((((((.....(((((((((..((((((((((....(((((...((.((.((.((((((((.((((......((....))..(((.((((((.((((((((.((((.((.....))..)))).)))))...))).)))))).))))))).)))))))))).)).))...(((..((((((...........(((((((.((((((......(((.....)))..(((..(((((......)).)))..))).)))))))))))))................((((((((....))))))))...(((.(((..(..(((.(((((........))))).)))..)....(((......)))....))).)))...))))))..))).)))))....((((.(((((..((((((((....))))).....)))..)).))).....))))...))))))...))))..)))))))))......))))))).)))..((((((((...(((((.((.(((((((((....))))..........))))).)).)))))........((((......))))..........)))).......)))).))).....)))))........... (-193.31) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 505 590 541 106 53 pint:PI2006|5end_potassium_uptake_system_protein_1964480..1964710_POSITIVE 497 590 544 73 14 pint:PI0301|5end_probable_transposase_285672..285902_POSITIVE 451 590 550 187 145 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 449 587 542 73 30 pint:PI0258|5end_conjugative_transposon_protein,_TraG_249722..249952_POSITIVE 440 581 544 70 33 pint:PI1921|5end_conserved_hypothetical_protein_1863917..1864147_POSITIVE 439 549 501 77 29 pint:PI1778|5end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1710009..1710239_POSITIVE 439 600 556 133 74 pint:PI0264|5end_transfer_region-related_protein,_TraN_256494..256724_POSITIVE 436 588 545 212 167 pint:PI2112|5end_phosphopantetheine_adenylyltransferase_2063194..2063424_POSITIVE 435 579 532 213 159 pint:PI1047|5end_conserved_hypothetical_protein_965614..965844_POSITIVE 425 245 201 186 132 pint:PI0733|5end_membrane_protein;_predicted_exporter_664389..664619_POSITIVE 419 216 180 193 151 pint:PI0909|5end_possible_mutT/NUDIX_family_protein_837526..837756_POSITIVE 417 130 81 65 21 pint:PI1796|5end_2-hydroxyhepta-2,4-diene-1,7-dioate_isomerase,_fumarylacetoacetate_hydrolase_family_protein_1730452..1730682_POSITIVE 415 603 561 214 162 pint:PI0646|5end_hypothetical_protein_586583..586813_POSITIVE 415 570 522 76 21 pint:PI0537|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_489756..489986_POSITIVE 414 597 552 68 12 pint:PI0744|5end_DNA_primase/mobilizable_transposon,_excision_protein_674171..674401_POSITIVE 413 589 541 199 151 pint:PI0571|5end_conserved_hypothetical_protein;_possible_permease_515161..515391_POSITIVE 410 578 531 205 142 pint:PI0608|5end_isochorismate_synthase_555487..555717_POSITIVE 404 560 512 149 104 pint:PI1705|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_III_1621781..1622011_POSITIVE 403 603 563 50 2 pint:PI0645|5end_hypothetical_protein_586419..586649_POSITIVE 401 580 531 43 2 pint:PI1639|5end_conserved_hypothetical_protein_1556725..1556955_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 497 590 544 82 23 pint:PI0300|3end_transposase_285761..285911_POSITIVE 461 568 525 56 6 pint:PI1647|3end_hypothetical_protein_1562531..1562681_POSITIVE 461 568 525 56 6 pint:PI0353|3end_hypothetical_protein_325337..325487_POSITIVE 449 587 542 73 30 pint:PI0257|3end_conjugative_transposon_protein,_TraF_249802..249952_POSITIVE 439 549 501 69 21 pint:PI1777|3end_conserved_hypothetical_protein_1710081..1710231_POSITIVE 439 569 525 98 47 pint:PI1716|3end_hypothetical_protein_1632006..1632156_POSITIVE 425 245 201 84 30 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 415 603 561 120 68 pint:PI0644|3end_dnaJ_protein_586569..586719_POSITIVE 414 579 532 147 93 pint:PI0043|3end_thiamine_phosphate_pyrophosphorylase_43156..43306_POSITIVE 404 567 523 151 98 pint:PI0675|3end_hypothetical_protein_613957..614107_POSITIVE 397 567 522 147 111 pint:PI0667|3end_hypothetical_protein_610477..610627_POSITIVE 390 590 547 150 106 pint:PI0595|3end_conserved_hypothetical_protein_546406..546556_POSITIVE 387 589 544 63 1 pint:PI1649|3end_hypothetical_protein_1563441..1563591_POSITIVE 387 589 541 78 20 pint:PI0166|3end_hypothetical_protein_161258..161408_POSITIVE 387 420 372 83 20 pint:PI0058|3end_conserved_hypothetical_protein_57044..57194_POSITIVE 386 242 211 138 108 pint:PI1542|3end_mobilization_protein_1462866..1463016_POSITIVE 383 570 522 145 95 pint:PI0692|3end_iron_compound_ABC_transporter,_permease_protein_628269..628419_POSITIVE 382 128 81 135 85 pint:PI1606|3end_conserved_hypothetical_protein_1535565..1535715_POSITIVE 381 235 192 137 97 pint:PI0911|3end_tyrosine_phenol-lyase_841209..841359_POSITIVE 378 222 181 67 17 pint:PI0245|3end_possible_mobilization_protein_244258..244408_POSITIVE