Origin IGS: taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataatttgtttcgggttttacagccgtctttacatctcctttcggatgattgcaaagactacctgcactgctgctgccactccaacaaaaggttaatgccagcaaaaatagaatgttttttctcattttagtaaaattcagacgagcctgaaataagctcaaagtgatttagataatattgttttaggcaaaattacacttttttattgagtaagaaaaattatataatgtcttttttcaaaaacacttcaattctgtttctcctacattctccaccttatatttataaaggaaaagacaataataaaagtgtta Mask Tandem Repeat Region ================================================ taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Find is-nt database================================================ Query_seq: PI0926:PI0927|PI0926:PI0927:hypothetical protein:transglutaminase-related protein:->->:857108..857422 315 taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0926:PI0927|PI0926:PI0927:hypothetical protein:transglutaminase-related protein:->->:857108..857422 315 taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0926:PI0927|PI0926:PI0927:hypothetical protein:transglutaminase-related protein:->->:857108..857422 315 taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Predict ORF larger than 30AA ================================================ Protein_Len: 56 Strand: + Start: 148 End: 315 ................................................................................................................................................... M T L S L F Q A R L N F T K M R K N I L F L L A L T F C W S G S S S A G S L C N H P K G D V K T A V K P E T N Y Protein_Len: 31 Strand: - Start: 223 End: 315 .............................................................................................................................................................................................................................. G K T P T A A A T C T T K A I M R F S I Y L R S Y F G F C I M Protein_Len: 41 Strand: - Start: 188 End: 310 ........................................................................................................................................................................................... F S F F C E I K A P M L R K N S H C C C H L Y D K C D D S L L H L S P Q L V R F M ..... Protein_Len: 33 Strand: - Start: 168 End: 266 ....................................................................................................................................................................... A R R F K V L I L F F M R N K S A N V K Q Q L P L L L A P L R Q M ................................................. Protein_Len: 36 Strand: - Start: 2 End: 109 . V S K N N D K G K Y I Y P P S H L L F L I S T N K F F S M I Y N K K S M .............................................................................................................................................................................................................. taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0926:PI0927|PI0926:PI0927:hypothetical protein:transglutaminase-related protein:->->:857108..857422 315 taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Intra-Species Hit: Count: 1 Min: 1 Max: 315 Len: 315 Subject: pint_PI0926_PI0927|hypothetical protein:transglutaminase-related protein|POSITIVE:POSITIVE|[857108,857422]|315 HSP 1 e-value: 1.0E-178 bit: 624.0 Len: 315 Query Start:1 Query End:315 Subject Strand: POSITIVE Subject Start: 1 Subject End: 315 taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat taacacttttattattgtcttttcctttataaatataaggtggagaatgtaggagaaacagaattgaagtgtttttgaaaaaagacattatataatttttcttactcaataaaaaagtgtaattttgcctaaaacaatattatctaaatcactttgagcttatttcaggctcgtctgaattttactaaaatgagaaaaaacattctatttttgctggcattaaccttttgttggagtggcagcagcagtgcaggtagtctttgcaatcatccgaaaggagatgtaaagacggctgtaaaacccgaaacaaattat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACUUUUAUUAUUGUCUUUUCCUUUAUAAAUAUAAGGUGGAGAAUGUAGGAGAAACAGAAUUGAAGUGUUUUUGAAAAAAGACAUUAUAUAAUUUUUCUUACUCAAUAAAAAAGUGUAAUUUUGCCUAAAACAAUAUUAUCUAAAUCACUUUGAGCUUAUUUCAGGCUCGUCUGAAUUUUACUAAAAUGAGAAAAAACAUUCUAUUUUUGCUGGCAUUAACCUUUUGUUGGAGUGGCAGCAGCAGUGCAGGUAGUCUUUGCAAUCAUCCGAAAGGAGAUGUAAAGACGGCUGUAAAACCCGAAACAAAUUAU ..((((((((.((((((((((..((((.........))))..))))..((((((((((......((.(((((((((....))))))))).))...)))))))))).)))))))))))))).....((((...................(((...(((((((.....)))))))..))).......(((((..((((......))))..)))))..)))).......((((((((.((.(((((.((...)).....(((((((((.((.(((....)))))))))))))).)))))...))))..)))))).... (-82.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAUUUGUUUCGGGUUUUACAGCCGUCUUUACAUCUCCUUUCGGAUGAUUGCAAAGACUACCUGCACUGCUGCUGCCACUCCAACAAAAGGUUAAUGCCAGCAAAAAUAGAAUGUUUUUUCUCAUUUUAGUAAAAUUCAGACGAGCCUGAAAUAAGCUCAAAGUGAUUUAGAUAAUAUUGUUUUAGGCAAAAUUACACUUUUUUAUUGAGUAAGAAAAAUUAUAUAAUGUCUUUUUUCAAAAACACUUCAAUUCUGUUUCUCCUACAUUCUCCACCUUAUAUUUAUAAAGGAAAAGACAAUAAUAAAAGUGUUA .....((((((.(((((((((....((((((.(((((((....))).)).)).))))))............(((((.((..((.......))....)).)))))....(((((((........))))))))))))))))))))))(((((((((((..(((((....))).)).....)))))))))))......((((((((((((((.(((.((((((..((.....))..))))))..(((((.........)))))....)))........((((.........))))......)))))).)))))))).. (-62.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 384 255 213 45 8 pint:PI0773|5end_D-3-phosphoglycerate_dehydrogenase_708498..708728_POSITIVE 367 279 234 164 112 pint:PI1448|5end_hypothetical_protein_1371579..1371809_POSITIVE 353 257 212 219 179 pint:PI0596|5end_hypothetical_protein_546400..546630_POSITIVE 352 258 211 95 17 pint:PI1985|5end_hypothetical_protein_1940291..1940521_POSITIVE 336 280 231 185 140 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 332 260 212 163 119 pint:PI1891|5end_hypothetical_protein_1835196..1835426_POSITIVE 332 266 221 48 2 pint:PI1723|5end_conserved_hypothetical_protein_1641649..1641879_POSITIVE 330 255 212 114 63 pint:PI0920|5end_xanthan_lyase_849688..849918_POSITIVE 326 280 231 218 164 pint:PI0034|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_37151..37381_POSITIVE 325 266 230 39 4 pint:PI1367|5end_redox-sensitive_transcriptional_activator,__OxyR_1286860..1287090_POSITIVE 322 254 212 198 158 pint:PI1038|5end_hypothetical_protein_958685..958915_POSITIVE 320 278 233 189 139 pint:PI1644|5end_hypothetical_protein_1560372..1560602_POSITIVE 320 254 211 193 137 pint:PI0251|5end_hypothetical_protein_247551..247781_POSITIVE 319 269 223 60 7 pint:PI1073|5end_hemogluttinin_A-related_protein_(HagA)_990593..990823_POSITIVE 319 260 214 132 83 pint:PI0834|5end_conserved_hypothetical_protein_770785..771015_POSITIVE 317 250 203 85 30 pint:PI1495|5end_conserved_hypothetical_protein_1414432..1414662_POSITIVE 317 252 213 117 73 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 310 257 211 169 127 pint:PI0733|5end_membrane_protein;_predicted_exporter_664389..664619_POSITIVE 305 274 232 94 39 pint:PI2013|5end_hypothetical_protein_1972329..1972559_POSITIVE 304 250 201 132 65 pint:PI1789|5end_hypothetical_protein_1718727..1718957_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 440 266 222 127 72 pint:PI0129|3end_hypothetical_protein_121941..122091_POSITIVE 356 281 242 53 8 pint:PI1815|3end_polyphosphate-selective_porin_O_1754510..1754660_POSITIVE 356 257 225 45 8 pint:PI0324|3end_conserved_hypothetical_protein_305680..305830_POSITIVE 353 294 253 149 111 pint:PI0935|3end_collagenase,_protease_868320..868470_POSITIVE 353 257 212 145 105 pint:PI0595|3end_conserved_hypothetical_protein_546406..546556_POSITIVE 330 255 212 65 14 pint:PI0919|3end_conserved_hypothetical_protein_849719..849869_POSITIVE 326 280 231 88 34 pint:PI0033|3end_peptidylprolyl_isomerase_37101..37251_POSITIVE 325 269 222 151 103 pint:PI1254|3end_probable_glucokinase_1176646..1176796_POSITIVE 310 257 211 67 25 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 309 259 215 121 73 pint:PI1380|3end_conserved_hypothetical_protein_1303242..1303392_POSITIVE 308 278 233 83 34 pint:PI1892|3end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_1835547..1835697_POSITIVE 305 257 211 79 32 pint:PI0047|3end_phosphomethylpyrimidine_kinase_46781..46931_POSITIVE 301 256 215 112 64 pint:PI1663|3end_hypothetical_protein_1577347..1577497_POSITIVE 301 253 213 130 85 pint:PI1075|3end_pyruvate_phosphate_dikinase_998571..998721_POSITIVE 300 265 225 148 108 pint:PI2062|3end_type_I_restriction-modification_system_2024008..2024158_POSITIVE 297 254 215 128 72 pint:PI0644|3end_dnaJ_protein_586569..586719_POSITIVE 294 265 224 103 60 pint:PI1568|3end_OmpA_family_protein_1491043..1491193_POSITIVE 294 260 211 82 24 pint:PI1284|3end_ATP_synthase,_delta_subunit_1200323..1200473_POSITIVE 294 273 232 45 7 pint:PI0028|3end_aminopeptidase_32727..32877_POSITIVE 293 280 231 88 50 pint:PI0908|3end_conserved_hypothetical_protein_837612..837762_POSITIVE