Origin IGS: aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaagtcgggaagcataaaaataccgccatcacggataaaaacatatctgttgatggcggttttttgttatatcactactgcaaaatcgctgcagcaacactataagctatatttcggaactgagaaattgagcgtgaacaactcactttatgagtgaaaaagaggcgagagtattgtaaatatttttcgcaagcataactattagctaaactgctcgttatcaacgcattacagaacctattgttttgcattccaaaagcgtaggtt Mask Tandem Repeat Region ================================================ aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Find is-nt database================================================ Query_seq: PI0905:PI0907|PI0905:PI0907:hypothetical protein:NAD-dependent DNA ligase:->->:834758..835026 269 aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0905:PI0907|PI0905:PI0907:hypothetical protein:NAD-dependent DNA ligase:->->:834758..835026 269 aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0905:PI0907|PI0905:PI0907:hypothetical protein:NAD-dependent DNA ligase:->->:834758..835026 269 aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Predict ORF larger than 30AA ================================================ Protein_Len: 58 Strand: + Start: 17 End: 190 ................ M Q N N R F C N A L I T S S L A N S Y A C E K Y L Q Y S R L F F T H K V S C S R S I S Q F R N I A Y S V A A A I L Q ............................................................................... Protein_Len: 47 Strand: - Start: 114 End: 254 ................................................................................................................. E Y L S N N V S L K E T G F Y L K Y H Q Q L S K A T T I Y C F V A M L L Y T K I R S P P I K M ............... aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 41 HP_score: -2.7 Tail_Score: -2.62184 Start: 171 End: 193 Full_Region: ATATAGCTTATAGTG TTGCTGCAG CGATT TTGCAGTAG TGATATAACAAAAAA ..........................................................................................................................................................................TTGCTGCAGCGATTTTGCAGTAG............................................................................ TransTerm Strand: + Conf: 86 HP_score: -7.7 Tail_Score: -4.98255 Start: 207 End: 247 Full_Region: AGTGATATAACAAAA AACCGCCATCAACAGATA TGTTTT TATCCGTGATGGCGGTA TTTTTATGCTTCCCG ..............................................................................................................................................................................................................AACCGCCATCAACAGATATGTTTTTATCCGTGATGGCGGTA...................... TransTerm Strand: - Conf: 100 HP_score: -12.5 Tail_Score: -5.60689 Start: 209 End: 245 Full_Region: ggaagcataaaaata ccgccatcacggata aaaaca tatctgttgatggcgg ttttttgttatatca ................................................................................................................................................................................................................ccgccatcacggataaaaacatatctgttgatggcgg........................ Find igs database================================================ Query_seq: PI0905:PI0907|PI0905:PI0907:hypothetical protein:NAD-dependent DNA ligase:->->:834758..835026 269 aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Intra-Species Hit: Count: 1 Min: 1 Max: 269 Len: 269 Subject: pint_PI0905_PI0907|hypothetical protein:NAD-dependent DNA ligase|POSITIVE:POSITIVE|[834758,835026]|269 HSP 1 e-value: 1.0E-150 bit: 533.0 Len: 269 Query Start:1 Query End:269 Subject Strand: POSITIVE Subject Start: 1 Subject End: 269 aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta aacctacgcttttggaatgcaaaacaataggttctgtaatgcgttgataacgagcagtttagctaatagttatgcttgcgaaaaatatttacaatactctcgcctctttttcactcataaagtgagttgttcacgctcaatttctcagttccgaaatatagcttatagtgttgctgcagcgattttgcagtagtgatataacaaaaaaccgccatcaacagatatgtttttatccgtgatggcggtatttttatgcttcccgactttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCUACGCUUUUGGAAUGCAAAACAAUAGGUUCUGUAAUGCGUUGAUAACGAGCAGUUUAGCUAAUAGUUAUGCUUGCGAAAAAUAUUUACAAUACUCUCGCCUCUUUUUCACUCAUAAAGUGAGUUGUUCACGCUCAAUUUCUCAGUUCCGAAAUAUAGCUUAUAGUGUUGCUGCAGCGAUUUUGCAGUAGUGAUAUAACAAAAAACCGCCAUCAACAGAUAUGUUUUUAUCCGUGAUGGCGGUAUUUUUAUGCUUCCCGACUUUUA .........((((((((((((.(((.....))).)))...((((.((((((..(((((.((((.....)))).)).)))...............................(((((.....))))))))))).))))...........)))))))))...(((.....((((..((((.(((....))).))))..))))...(((((((((((((((...((((......))))..)))))))))).)))))..)))............ (-67.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGUCGGGAAGCAUAAAAAUACCGCCAUCACGGAUAAAAACAUAUCUGUUGAUGGCGGUUUUUUGUUAUAUCACUACUGCAAAAUCGCUGCAGCAACACUAUAAGCUAUAUUUCGGAACUGAGAAAUUGAGCGUGAACAACUCACUUUAUGAGUGAAAAAGAGGCGAGAGUAUUGUAAAUAUUUUUCGCAAGCAUAACUAUUAGCUAAACUGCUCGUUAUCAACGCAUUACAGAACCUAUUGUUUUGCAUUCCAAAAGCGUAGGUU ..(((((.(((....(((((((.((((((((((((((((......)))))).)))))))))))))))))....((((..(((((.......)))))...........(((..(((((........)))))..)))))))....))))))))....((((((((...((((.....)))).....))))))))..(((((((....(((......))).)))))....)).......(((((((.(((((........)))))))))))) (-70.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 698 50 1 163 114 pint:PI0157|5end_hypothetical_protein_151185..151415_POSITIVE 675 50 1 86 37 pint:PI0200|5end_hypothetical_protein_191545..191775_POSITIVE 662 50 1 97 48 pint:PI0755|5end_hypothetical_protein_686691..686921_POSITIVE 656 46 1 82 37 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 643 50 1 114 65 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 632 49 7 113 71 pint:PI1934|5end_hypothetical_protein_1882363..1882593_POSITIVE 626 46 1 137 92 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 606 50 1 86 37 pint:PI2131|5end_hypothetical_protein_2080678..2080908_POSITIVE 603 49 7 96 54 pint:PI1666|5end_conserved_hypothetical_protein_1579231..1579461_POSITIVE 597 46 1 137 92 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 596 54 11 118 75 pint:PI1567|5end_hypothetical_protein_1489229..1489459_POSITIVE 594 50 1 71 22 pint:PI1905|5end_hypothetical_protein_1847628..1847858_POSITIVE 582 41 1 231 191 pint:PI2111|5end_conserved_hypothetical_protein_2062771..2063001_POSITIVE 581 50 1 163 114 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 581 50 1 75 26 pint:PI0053|5end_hypothetical_protein_54727..54957_POSITIVE 575 46 1 110 65 pint:PI1694|5end_hypothetical_protein_1609717..1609947_POSITIVE 571 59 11 124 75 pint:PI2002|5end_hypothetical_protein_1960662..1960892_POSITIVE 557 49 1 85 37 pint:PI0004|5end_hypothetical_protein_6199..6429_POSITIVE 550 49 1 74 26 pint:PI0099|5end_hypothetical_protein_95530..95760_POSITIVE 541 48 7 43 2 pint:PI1922|5end_hypothetical_protein_1865219..1865449_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 657 47 2 46 1 pint:PI1391|3end_hypothetical_protein_1311694..1311844_POSITIVE 619 50 1 69 20 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 581 50 1 97 48 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 579 50 7 49 6 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE 565 50 7 65 22 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 562 48 1 67 20 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 561 50 1 63 14 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 543 45 1 151 107 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 520 59 11 52 3 pint:PI2143|3end_DNA_mismatch_repair_protein_2091974..2092124_POSITIVE 511 59 18 49 7 pint:PI2130|3end_DNA_polymerase_I_2080711..2080861_POSITIVE 510 45 1 151 107 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 402 46 1 93 48 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 393 54 22 33 1 pint:PI1566|3end_predicted_TPR-repeat-containing_protein_1489224..1489374_POSITIVE 366 246 201 49 4 pint:PI2165|3end_conserved_hypothetical_protein;_possible_membrane_protein_2111205..2111355_POSITIVE 351 70 24 47 1 pint:PI1600|3end_conserved_hypothetical_protein_1530275..1530425_POSITIVE 348 90 42 49 1 pint:PI0412|3end_primosomal_protein_N',_replication_factor_Y_382152..382302_POSITIVE 343 105 61 72 29 pint:PI1117|3end_glycosyl_transferase,_family_2_1042279..1042429_POSITIVE 328 189 142 108 69 pint:PI0020|3end_conserved_hypothetical_protein_24955..25105_POSITIVE 325 197 152 55 10 pint:PI1743|3end_stationary-phase_survival_protein_1664930..1665080_POSITIVE 307 245 209 59 24 pint:PI0910|3end_possible_sodium_hydrogen_antiporter_(Na+/H+_antiporter)_839544..839694_POSITIVE