Origin IGS: tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttcttctgacactattttaaaatatcaatctttacagggtgcgaaattatacattatttccaaaacaagcaagggttcggtttaattttattcgttaatgattttcatcagactataaaaactatgaacaacgaaaaaaagttacgttttttcttgttgttatttaccttatttattatctttgcatatatttcgaaaaagtacactataatata Mask Tandem Repeat Region ================================================ tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Find is-nt database================================================ Query_seq: PI0900:PI0901|PI0900:PI0901:signal recognition particle protein:hypothetical protein:->->:831153..831367 215 tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0900:PI0901|PI0900:PI0901:signal recognition particle protein:hypothetical protein:->->:831153..831367 215 tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0900:PI0901|PI0900:PI0901:signal recognition particle protein:hypothetical protein:->->:831153..831367 215 tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 4 End: 105 ... M I V Y F F E I Y A K I I N K V N N N K K K R N F F S L F I V F I V .............................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 194 .. K K S I Y A F I I F L T F L L L F F R L K K E N N M T K I T Q H F D N V F L I L G F G Q K N Q F Y H I I E C G T F I S M ..................... tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0900:PI0901|PI0900:PI0901:signal recognition particle protein:hypothetical protein:->->:831153..831367 215 tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Intra-Species Hit: Count: 1 Min: 1 Max: 215 Len: 215 Subject: pint_PI0900_PI0901|signal recognition particle protein:hypothetical protein|POSITIVE:POSITIVE|[831153,831367]|215 HSP 1 e-value: 1.0E-106 bit: 385.0 Len: 215 Query Start:1 Query End:215 Subject Strand: POSITIVE Subject Start: 1 Subject End: 215 tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaacnnnnnnncgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa tatattatagtgtactttttcgaaatatatgcaaagataataaataaggtaaataacaacaagaaaaaacgtaactttttttcgttgttcatagtttttatagtctgatgaaaatcattaacgaataaaattaaaccgaacccttgcttgttttggaaataatgtataatttcgcaccctgtaaagattgatattttaaaatagtgtcagaagaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAUUAUAGUGUACUUUUUCGAAAUAUAUGCAAAGAUAAUAAAUAAGGUAAAUAACAACAAGAAAAAACGUAACUUUUUUUCGUUGUUCAUAGUUUUUAUAGUCUGAUGAAAAUCAUUAACGAAUAAAAUUAAACCGAACCCUUGCUUGUUUUGGAAAUAAUGUAUAAUUUCGCACCCUGUAAAGAUUGAUAUUUUAAAAUAGUGUCAGAAGAA ....(((((((((((.(((((((((((...((((....................((((((.(((((((.......))))))).))))))..(((((((....((.(((((.....)))))))....)))))))..........)))).)))))))))))....)))))..........))))))...((((((((.......))))))))..... (-35.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUUCUGACACUAUUUUAAAAUAUCAAUCUUUACAGGGUGCGAAAUUAUACAUUAUUUCCAAAACAAGCAAGGGUUCGGUUUAAUUUUAUUCGUUAAUGAUUUUCAUCAGACUAUAAAAACUAUGAACAACGAAAAAAAGUUACGUUUUUUCUUGUUGUUAUUUACCUUAUUUAUUAUCUUUGCAUAUAUUUCGAAAAAGUACACUAUAAUAUA ..........(((.(((..((((((...........(..((.(((((........)))))))...)..((((((((.........((((((..(((..((((....))))))).))))))......((((((((.((((((.....)))))).))))))))................))))))))..))))))..))).)))............. (-30.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 281 186 142 50 3 pint:PI0272|5end_conserved_hypothetical_protein_262621..262851_POSITIVE 255 90 42 154 104 pint:PI0625|5end_possible_membrane_transport_protein_573104..573334_POSITIVE 254 187 142 139 97 pint:PI1769|5end_hypothetical_protein_1696507..1696737_POSITIVE 237 182 141 79 37 pint:PI1854|5end_possible_glycosyl_transferase_1800925..1801155_POSITIVE 234 79 31 215 170 pint:PI0434|5end_possible_beta-lactamase_405692..405922_POSITIVE 233 179 137 132 78 pint:PI0439|5end_conserved_hypothetical_protein;_possible_preprotein_translocase_subunit_SecE_410319..410549_POSITIVE 228 159 115 68 25 pint:PI0881|5end_conserved_hypothetical_protein_814916..815146_POSITIVE 223 67 33 105 70 pint:PI0786|5end_conserved_hypothetical_protein;_possible_glycosyltransferase_723240..723470_POSITIVE 221 147 101 81 21 pint:PI0030|5end_transcriptional_regulator,_AsnC_family_34509..34739_POSITIVE 217 175 135 109 70 pint:PI1724|5end_inosine-5'-monophosphate_dehydrogenase_1641075..1641305_POSITIVE 216 187 142 87 42 pint:PI1322|5end_nucleoside_permease_1240324..1240554_POSITIVE 213 182 145 204 169 pint:PI1006|5end_conserved_hypothetical_protein_926604..926834_POSITIVE 211 179 135 189 144 pint:PI0393|5end_conserved_hypothetical_protein_358696..358926_POSITIVE 211 175 144 229 193 pint:PI0069|5end_hypothetical_protein_67072..67302_POSITIVE 210 183 142 59 18 pint:PI1331|5end_conserved_hypothetical_protein_1248442..1248672_POSITIVE 210 178 135 64 17 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 209 180 145 82 40 pint:PI0606|5end_naphthoate_synthase_552956..553186_POSITIVE 206 157 131 228 199 pint:PI1634|5end_conserved_hypothetical_protein_1553179..1553409_POSITIVE 206 179 135 168 121 pint:PI1332|5end_aminotransferase,_possible_pyridoxal-phosphate-dependent_aminotransferase_1249374..1249604_POSITIVE 206 57 11 144 96 pint:PI0704|5end_conserved_hypothetical_protein_640247..640477_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 106 66 54 15 pint:PI1555|3end_conserved_hypothetical_protein_1471754..1471904_POSITIVE 246 90 43 50 1 pint:PI0623|3end_hypothetical_protein_573080..573230_POSITIVE 243 184 144 87 42 pint:PI0559|3end_conserved_hypothetical_protein_508740..508890_POSITIVE 228 159 115 68 25 pint:PI0880|3end_hypothetical_protein_814996..815146_POSITIVE 223 67 33 88 53 pint:PI0784|3end_possible_amidase_enhancer_precursor_723303..723453_POSITIVE 216 187 142 84 39 pint:PI1321|3end_conserved_hypothetical_protein_1240401..1240551_POSITIVE 215 103 70 74 39 pint:PI0876|3end_hypothetical_protein_811035..811185_POSITIVE 214 181 144 104 60 pint:PI0068|3end_arginine_repressor_67019..67169_POSITIVE 211 179 135 148 103 pint:PI0392|3end_possible_transcriptional_regulator_358735..358885_POSITIVE 209 180 145 72 30 pint:PI0605|3end_chloromuconate_cycloisomerase_553026..553176_POSITIVE 203 179 136 136 83 pint:PI0998|3end_possible_flavin_reductase_domain_protein_923336..923486_POSITIVE 201 210 161 150 102 pint:PI1024|3end_conserved_hypothetical_protein;_possible_nitroreductase_family_protein_940732..940882_POSITIVE 201 78 31 89 42 pint:PI0452|3end_conserved_hypothetical_protein;_possible_surface_antigen_426791..426941_POSITIVE 196 108 67 138 97 pint:PI1256|3end_conserved_hypothetical_protein_1178109..1178259_POSITIVE 196 69 29 106 68 pint:PI0423|3end_hypothetical_protein_388432..388582_POSITIVE 195 164 137 136 105 pint:PI0116|3end_conserved_hypothetical_protein_110769..110919_POSITIVE 194 107 76 149 115 pint:PI0753|3end_hypothetical_protein_683851..684001_POSITIVE 193 178 143 151 115 pint:PI0863|3end_L-asparaginase_I_800563..800713_POSITIVE 190 182 141 62 26 pint:PI1484|3end_conserved_hypothetical_protein_1405223..1405373_POSITIVE 190 50 5 74 26 pint:PI1311|3end_large_protein_related_to_C.hutchinsonii_large_proteins_1227439..1227589_POSITIVE