Origin IGS: ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatattagttacttctatattttacaagagacccatagctaaaatgctaacgggtctcttttttatatatctgtttggtaatgcaaagaactgctataaaacaaataattaaacactttttatagcaattcgtcccattttatacctaaaaatgcacttttcagacacttttcagcaaaaacatttggttat Mask Tandem Repeat Region ================================================ ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Find is-nt database================================================ Query_seq: PI0893:PI0894|PI0893:PI0894:outer membrane protein 41 precursor:2-oxoglutarate oxidoreductase, gamma subunit (2-oxoglutarate-ferredoxin oxidoreductase):->->:825723..825915 193 ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0893:PI0894|PI0893:PI0894:outer membrane protein 41 precursor:2-oxoglutarate oxidoreductase, gamma subunit (2-oxoglutarate-ferredoxin oxidoreductase):->->:825723..825915 193 ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0893:PI0894|PI0893:PI0894:outer membrane protein 41 precursor:2-oxoglutarate oxidoreductase, gamma subunit (2-oxoglutarate-ferredoxin oxidoreductase):->->:825723..825915 193 ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 84 End: 191 ................................................................................... N N T K Y C N K A N G F L Y I F F L G T L M K A I P R K Y F I S T V L M .. ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 72 HP_score: -8.4 Tail_Score: -3.34962 Start: 135 End: 165 Full_Region: CAGATATATAAAAAA GAGACCCGTTAGC ATTTTA GCTATGGGTCTC TTGTAAAATATAGAA ......................................................................................................................................GAGACCCGTTAGCATTTTAGCTATGGGTCTC............................ Find igs database================================================ Query_seq: PI0893:PI0894|PI0893:PI0894:outer membrane protein 41 precursor:2-oxoglutarate oxidoreductase, gamma subunit (2-oxoglutarate-ferredoxin oxidoreductase):->->:825723..825915 193 ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Intra-Species Hit: Count: 1 Min: 1 Max: 193 Len: 193 Subject: pint_PI0893_PI0894|outer membrane protein 41 precursor:2-oxoglutarate oxidoreductase, gamma subunit (2-oxoglutarate-ferredoxin oxidoreductase)|POSITIVE:POSITIVE|[825723,825915]|193 HSP 1 e-value: 1.0E-105 bit: 383.0 Len: 193 Query Start:1 Query End:193 Subject Strand: POSITIVE Subject Start: 1 Subject End: 193 ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta ataaccaaatgtttttgctgaaaagtgtctgaaaagtgcatttttaggtataaaatgggacgaattgctataaaaagtgtttaattatttgttttatagcagttctttgcattaccaaacagatatataaaaaagagacccgttagcattttagctatgggtctcttgtaaaatatagaagtaactaatatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAACCAAAUGUUUUUGCUGAAAAGUGUCUGAAAAGUGCAUUUUUAGGUAUAAAAUGGGACGAAUUGCUAUAAAAAGUGUUUAAUUAUUUGUUUUAUAGCAGUUCUUUGCAUUACCAAACAGAUAUAUAAAAAAGAGACCCGUUAGCAUUUUAGCUAUGGGUCUCUUGUAAAAUAUAGAAGUAACUAAUAUUA .......((((((.(((((.((((((((((....)).)))))))).((((....(((.((.((((((((((((((...((......))...)))))))))))))).)).))))))).......(((((....(((((((((((.(((......)))))))))))))).....)))))..)))))..)))))). (-57.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUUAGUUACUUCUAUAUUUUACAAGAGACCCAUAGCUAAAAUGCUAACGGGUCUCUUUUUUAUAUAUCUGUUUGGUAAUGCAAAGAACUGCUAUAAAACAAAUAAUUAAACACUUUUUAUAGCAAUUCGUCCCAUUUUAUACCUAAAAAUGCACUUUUCAGACACUUUUCAGCAAAAACAUUUGGUUAU ........(((.....(((((.....(((((((((.((((......))))..)))))))))....)))))..(((((((...((((..(((.((((((((((................)))))))))).)))......(((((....))))).))))....)))))))......)))................ (-41.19) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 267 65 25 230 190 pint:PI0243|5end_hypothetical_protein_240506..240736_POSITIVE 252 156 115 87 41 pint:PI0889|5end_pyruvate_kinase_818758..818988_POSITIVE 242 74 34 76 38 pint:PI1396|5end_urocanate_hydratase_1315799..1316029_POSITIVE 240 162 124 196 158 pint:PI1528|5end_hypothetical_protein_1449685..1449915_POSITIVE 237 148 103 64 17 pint:PI1949|5end_aminotransferase,_class_V_1903218..1903448_POSITIVE 237 40 3 97 69 pint:PI1702|5end_hypothetical_protein_1620587..1620817_POSITIVE 230 160 115 66 19 pint:PI1343|5end_conserved_hypothetical_protein_1257782..1258012_POSITIVE 228 180 133 162 114 pint:PI1791|5end_conserved_hypothetical_protein_1718489..1718719_POSITIVE 228 180 133 154 106 pint:PI1788|5end_hypothetical_protein_1718481..1718711_POSITIVE 228 180 133 87 39 pint:PI1787|5end_conserved_hypothetical_protein_1718414..1718644_POSITIVE 228 170 121 196 151 pint:PI0815|5end_hypothetical_protein_754475..754705_POSITIVE 225 59 14 116 59 pint:PI1799|5end_30S_ribosomal_protein_S2_1732900..1733130_POSITIVE 225 174 136 215 171 pint:PI0518|5end_5'-nucleotidase_precursor_477076..477306_POSITIVE 224 44 3 100 53 pint:PI0592|5end_DNA_mismatch_repair_protein_540795..541025_POSITIVE 224 169 136 118 84 pint:PI0206|5end_TPR-repeat-containing_protein_198112..198342_POSITIVE 223 169 141 63 39 pint:PI0146|5end_transcription_elongation_factor_140338..140568_POSITIVE 222 40 5 68 37 pint:PI1609|5end_Na+/H+_anti-porter_1536058..1536288_POSITIVE 221 68 30 180 133 pint:PI1983|5end_possible_xylanase_1939151..1939381_POSITIVE 220 49 2 152 99 pint:PI1313|5end_possible_POT_family_(proton-dependent_oligopeptide_transport_family)_1229710..1229940_POSITIVE 220 49 5 165 119 pint:PI0853|5end_glycosyl_transferase,_group_2_family_protein_785515..785745_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 74 34 59 21 pint:PI1394|3end_glutamate_formiminotransferase_1315862..1316012_POSITIVE 237 168 131 107 69 pint:PI0456|3end_30S_ribosomal_protein_S12_429219..429369_POSITIVE 228 170 121 67 22 pint:PI0814|3end_conserved_hypothetical_protein_754426..754576_POSITIVE 227 40 3 130 102 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 225 174 136 143 99 pint:PI0517|3end_apparent_PorT_homolog_477084..477234_POSITIVE 219 45 4 70 29 pint:PI0548|3end_possible_acetylxylan_esterase_500571..500721_POSITIVE 217 65 27 101 63 pint:PI0314|3end_conserved_hypothetical_protein_296678..296828_POSITIVE 213 60 14 121 81 pint:PI1607|3end_ABC-related_lipoprotein_releasing_system,_ATP-binding_protein_1535834..1535984_POSITIVE 212 62 30 148 110 pint:PI1982|3end_conserved_hypothetical_protein_1939208..1939358_POSITIVE 208 69 24 110 73 pint:PI1683|3end_integral_membrane_protein_1600898..1601048_POSITIVE 207 80 34 131 89 pint:PI1282|3end_ATP_synthase,_gamma_subunit_(F0F1-ATPase,_gamma_subunit)_1198269..1198419_POSITIVE 207 40 8 41 6 pint:PI1061|3end_conserved_hypothetical_protein_980073..980223_POSITIVE 205 111 76 118 83 pint:PI1044|3end_excinuclease_ABC,_subunit_A_964639..964789_POSITIVE 204 117 72 130 95 pint:PI1383|3end_biopolymer_transport_protein_tolQ_related_1305040..1305190_POSITIVE 204 59 11 94 43 pint:PI1227|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148974..1149124_POSITIVE 204 120 75 130 82 pint:PI0633|3end_possible_preprotein_translocase,_YajC_subunit_580080..580230_POSITIVE 203 59 11 80 38 pint:PI1000|3end_conserved_hypothetical_protein_923672..923822_POSITIVE 202 167 141 132 105 pint:PI1409|3end_conserved_hypothetical_protein_1331561..1331711_POSITIVE 200 70 24 101 62 pint:PI1844|3end_hypothetical_protein_1790279..1790429_POSITIVE 200 110 64 92 46 pint:PI1631|3end_hypothetical_protein_1552797..1552947_POSITIVE