Origin IGS: taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagcttaaatgtaggggtgcgattcatcgccttcatcatatttaggctgctttttctttggtgtaggaacgtgatgaatcacgcccttacgtgccggtgtgcattataaattcctcaaaaaacagaactgccccacagctttcattcatcgaaaccaagcctatggcatta Mask Tandem Repeat Region ================================================ taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Find is-nt database================================================ Query_seq: PI0865:PI0866|PI0865:PI0866:possible phosphoglycerol transferase:hypothetical protein:->->:803745..803915 171 taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0865:PI0866|PI0865:PI0866:possible phosphoglycerol transferase:hypothetical protein:->->:803745..803915 171 taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0865:PI0866|PI0865:PI0866:possible phosphoglycerol transferase:hypothetical protein:->->:803745..803915 171 taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 66 End: 155 ................................................................. L A C R C T L A H N M V N R C W L F L L R F I I F A I F R M ................ Protein_Len: 31 Strand: - Start: 2 End: 94 . I G Y A Q N R H I F A T P C N Q K K L F K Y H V G A R L P T M ............................................................................. taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0865:PI0866|PI0865:PI0866:possible phosphoglycerol transferase:hypothetical protein:->->:803745..803915 171 taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Intra-Species Hit: Count: 1 Min: 1 Max: 171 Len: 171 Subject: pint_PI0865_PI0866|possible phosphoglycerol transferase:hypothetical protein|POSITIVE:POSITIVE|[803745,803915]|171 HSP 1 e-value: 2.0E-92 bit: 339.0 Len: 171 Query Start:1 Query End:171 Subject Strand: POSITIVE Subject Start: 1 Subject End: 171 taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta taatgccataggcttggtttcgatgaatgaaagctgtggggcagttctgttttttgaggaatttataatgcacaccggcacgtaagggcgtgattcatcacgttcctacaccaaagaaaaagcagcctaaatatgatgaaggcgatgaatcgcacccctacatttaagcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGCCAUAGGCUUGGUUUCGAUGAAUGAAAGCUGUGGGGCAGUUCUGUUUUUUGAGGAAUUUAUAAUGCACACCGGCACGUAAGGGCGUGAUUCAUCACGUUCCUACACCAAAGAAAAAGCAGCCUAAAUAUGAUGAAGGCGAUGAAUCGCACCCCUACAUUUAAGCUA ..........(((((((...............((((((..(((.....(((((....)))))......))).)).))))..(((.(((.((((((((((..((....))...............((((...........)))))))))))))).))).)))..))))))). (-44.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUUAAAUGUAGGGGUGCGAUUCAUCGCCUUCAUCAUAUUUAGGCUGCUUUUUCUUUGGUGUAGGAACGUGAUGAAUCACGCCCUUACGUGCCGGUGUGCAUUAUAAAUUCCUCAAAAAACAGAACUGCCCCACAGCUUUCAUUCAUCGAAACCAAGCCUAUGGCAUUA ..(((...(((((((((((.((((((((((.(((..(((....(((........)))...)))...))).)))))))))).)))))))))))...)))..............................((((.....((((...(((...)))...))))....))))... (-46.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 482 117 72 226 186 pint:PI0648|5end_hypothetical_protein_589448..589678_POSITIVE 450 125 82 230 186 pint:PI1750|5end_sulfate_transporter_1672134..1672364_POSITIVE 402 119 76 121 75 pint:PI0933|5end_hypothetical_protein_865700..865930_POSITIVE 377 120 71 139 89 pint:PI2145|5end_hypothetical_protein_2092213..2092443_POSITIVE 360 47 4 82 33 pint:PI1825|5end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_1764996..1765226_POSITIVE 354 119 73 178 121 pint:PI0902|5end_hypothetical_protein_832291..832521_POSITIVE 348 126 82 225 180 pint:PI1689|5end_conserved_hypothetical_protein_1604310..1604540_POSITIVE 334 46 9 191 156 pint:PI0508|5end_possible_cell_filamentation_protein_466463..466693_POSITIVE 333 50 5 137 84 pint:PI1785|5end_conserved_hypothetical_protein_1717122..1717352_POSITIVE 312 112 74 213 181 pint:PI2064|5end_conserved_hypothetical_protein;_possible_type_I_restriction-modification_system,_S_subunit_2025076..2025306_POSITIVE 308 49 4 231 177 pint:PI1929|5end_conserved_hypothetical_protein;_possible_ankyrin_repeat_protein_(eukaryotic-like)_1873387..1873617_POSITIVE 307 141 101 114 77 pint:PI1343|5end_conserved_hypothetical_protein_1257782..1258012_POSITIVE 304 50 1 66 16 pint:PI0665|5end_dihydroorotate_dehydrogenase_607865..608095_POSITIVE 302 110 66 117 73 pint:PI2049|5end_possible_transcriptional_regulator,_AraC_family_2002502..2002732_POSITIVE 299 112 74 204 176 pint:PI2074|5end_conserved_hypothetical_protein_2033660..2033890_POSITIVE 298 110 77 154 125 pint:PI0367|5end_hypothetical_protein_337884..338114_POSITIVE 298 105 67 139 99 pint:PI0020|5end_conserved_hypothetical_protein_24525..24755_POSITIVE 297 44 9 122 75 pint:PI0123|5end_outer_membrane_protein,_ompH_family_113906..114136_POSITIVE 296 96 55 172 132 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 295 79 33 115 69 pint:PI1485|5end_hypothetical_protein_1405170..1405400_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 418 125 81 55 8 pint:PI1820|3end_hypothetical_protein_1760377..1760527_POSITIVE 377 120 71 146 96 pint:PI2144|3end_hypothetical_protein_2092300..2092450_POSITIVE 346 110 69 74 34 pint:PI1440|3end_conserved_hypothetical_protein_1363009..1363159_POSITIVE 336 108 69 86 44 pint:PI1884|3end_conserved_hypothetical_protein_1827457..1827607_POSITIVE 333 50 5 130 77 pint:PI1784|3end_possible_dTDP-glucose_4,6-dehydratase,_NAD-dependent_epimerase/dehydratase_family_1717195..1717345_POSITIVE 307 141 101 51 14 pint:PI1342|3end_conserved_hypothetical_protein_1257799..1257949_POSITIVE 302 110 66 101 57 pint:PI2048|3end_possible_two-component_system_sensor_histidine_kinase_2002566..2002716_POSITIVE 301 110 69 98 46 pint:PI1818|3end_hypothetical_protein_1757270..1757420_POSITIVE 298 110 77 84 55 pint:PI0366|3end_conserved_hypothetical_protein_337894..338044_POSITIVE 295 79 33 88 42 pint:PI1484|3end_conserved_hypothetical_protein_1405223..1405373_POSITIVE 288 46 4 145 103 pint:PI0835|3end_conserved_hypothetical_protein;_possible_protein-export_membrane_protein_771859..772009_POSITIVE 287 147 101 50 6 pint:PI1068|3end_nucleolar_protein,_Sun_(Fmu)_family_protein;_tRNA/rRNA_cytosine-C5-methylase_986166..986316_POSITIVE 286 110 76 52 19 pint:PI2088|3end_conserved_hypothetical_protein;_possible_PAP2_superfamily_protein_2045815..2045965_POSITIVE 283 60 13 83 36 pint:PI0925|3end_ribosomal_protein_L11_methyltransferase_856638..856788_POSITIVE 280 50 5 137 103 pint:PI0832|3end_sigma-54-dependent_transcriptional_regulator_770155..770305_POSITIVE 274 111 71 69 26 pint:PI1003|3end_conserved_hypothetical_protein_925718..925868_POSITIVE 272 50 4 56 9 pint:PI1827|3end_peptidase,_M23/M37_family,_N-terminal_1768602..1768752_POSITIVE 270 59 15 55 4 pint:PI0671|3end_hypothetical_protein_611807..611957_POSITIVE 269 42 3 142 90 pint:PI1996|3end_hypothetical_protein_1950588..1950738_POSITIVE 268 107 70 130 86 pint:PI2137|3end_RNA_methylase_SpoU_family_2083807..2083957_POSITIVE