Origin IGS: aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gctcgtattatactttgtggcaaagatagtaatttatttttagtaattatctatttacttccttcttttttctcgcttgctattaaatcgttacacgctgtttctttctataagcaggcaaaaataaacaagtgtaggactccaagatacgacaaacaaaaagataggtataaaattgatacaaatcaattactttacagcaacataatgatagtagaataatttttcattactacaaggtatttttcttgcttccattgcaaaagtcaagcattcttttgccgttctcaaaaaataatagtatttttgcaacataattacggtggaggatttataacgaataaaacaggccacctcagcaaaaaagaaataacgttattgcacttattatttt Mask Tandem Repeat Region ================================================ aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Find is-nt database================================================ Query_seq: PI0862:PI0863|PI0862:PI0863:polysaccharide export outer membrane protein:L-asparaginase I:->->:799183..799576 394 aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0862:PI0863|PI0862:PI0863:polysaccharide export outer membrane protein:L-asparaginase I:->->:799183..799576 394 aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0862:PI0863|PI0862:PI0863:polysaccharide export outer membrane protein:L-asparaginase I:->->:799183..799576 394 aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 64 End: 159 ............................................................... M L H R N Y V A K I L L F F E N G K R M L D F C N G S K K N T L ........................................................................................................................................................................................................................................... Protein_Len: 54 Strand: + Start: 41 End: 202 ........................................ M A C F I R Y K S S T V I M L Q K Y Y Y F L R T A K E C L T F A M E A R K I P C S N E K L F Y Y H Y V A V K ................................................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 206 End: 301 ............................................................................................................................................................................................................. M I C I N F I P I F L F V V S W S P T L V Y F C L L I E R N S V ............................................................................................. Protein_Len: 46 Strand: + Start: 172 End: 309 ........................................................................................................................................................................... M I L L S L C C C K V I D L Y Q F Y T Y L F V C R I L E S Y T C L F L P A Y R K K Q R V T I ..................................................................................... Protein_Len: 60 Strand: - Start: 176 End: 388 ............................................................................................................................................................................... N T D I K Y R D K Q K D Y R P T R C K N I K A Q K Y F S V A H L S K I A L S F F S P L L Y I I V L F L N S D K G C L I M ...... Protein_Len: 42 Strand: - Start: 81 End: 206 ................................................................................ T A F I S N N K S F P L L I S S K Q L P L L F F V K Y Y H F I I R S D N H Q Q L T M ............................................................................................................................................................................................ Protein_Len: 55 Strand: - Start: 2 End: 166 . F L L H L L T I E K K S L H G T K N T I F G G G Y N H Q L F V I I K Q S R C F F A Q S K C H F C S F Y R T T M .................................................................................................................................................................................................................................... aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0862:PI0863|PI0862:PI0863:polysaccharide export outer membrane protein:L-asparaginase I:->->:799183..799576 394 aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Intra-Species Hit: Count: 1 Min: 1 Max: 394 Len: 394 Subject: pint_PI0862_PI0863|polysaccharide export outer membrane protein:L-asparaginase I|POSITIVE:POSITIVE|[799183,799576]|394 HSP 1 e-value: 0.0 bit: 781.0 Len: 394 Query Start:1 Query End:394 Subject Strand: POSITIVE Subject Start: 1 Subject End: 394 aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc aaaataataagtgcaataacgttatttcttttttgctgaggtggcctgttttattcgttataaatcctccaccgtaattatgttgcaaaaatactattattttttgagaacggcaaaagaatgcttgacttttgcaatggaagcaagaaaaataccttgtagtaatgaaaaattattctactatcattatgttgctgtaaagtaattgatttgtatcaattttatacctatctttttgtttgtcgtatcttggagtcctacacttgtttatttttgcctgcttatagaaagaaacagcgtgtaacgatttaatagcaagcgagaaaaaagaaggaagtaaatagataattactaaaaataaattactatctttgccacaaagtataatacgagc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAUAAUAAGUGCAAUAACGUUAUUUCUUUUUUGCUGAGGUGGCCUGUUUUAUUCGUUAUAAAUCCUCCACCGUAAUUAUGUUGCAAAAAUACUAUUAUUUUUUGAGAACGGCAAAAGAAUGCUUGACUUUUGCAAUGGAAGCAAGAAAAAUACCUUGUAGUAAUGAAAAAUUAUUCUACUAUCAUUAUGUUGCUGUAAAGUAAUUGAUUUGUAUCAAUUUUAUACCUAUCUUUUUGUUUGUCGUAUCUUGGAGUCCUACACUUGUUUAUUUUUGCCUGCUUAUAGAAAGAAACAGCGUGUAACGAUUUAAUAGCAAGCGAGAAAAAAGAAGGAAGUAAAUAGAUAAUUACUAAAAAUAAAUUACUAUCUUUGCCACAAAGUAUAAUACGAGC .(((((((..((......))))))))).((((((((((.(((((.....(((((.....)))))....)))))............((((((.........))))))....))))))))))..(((((..((((((.......))))))...((((.((((.(((((((...............)))))))......((((((....((((((((................(((((((.(((.((((..((..(((((.(((((((((((.((((((........)))))).)))))).)))))..)))))...))...)))).))).))))))).(((((........)))))....))))))))....)))))).)))).))))....))))) (-76.76) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUCGUAUUAUACUUUGUGGCAAAGAUAGUAAUUUAUUUUUAGUAAUUAUCUAUUUACUUCCUUCUUUUUUCUCGCUUGCUAUUAAAUCGUUACACGCUGUUUCUUUCUAUAAGCAGGCAAAAAUAAACAAGUGUAGGACUCCAAGAUACGACAAACAAAAAGAUAGGUAUAAAAUUGAUACAAAUCAAUUACUUUACAGCAACAUAAUGAUAGUAGAAUAAUUUUUCAUUACUACAAGGUAUUUUUCUUGCUUCCAUUGCAAAAGUCAAGCAUUCUUUUGCCGUUCUCAAAAAAUAAUAGUAUUUUUGCAACAUAAUUACGGUGGAGGAUUUAUAACGAAUAAAACAGGCCACCUCAGCAAAAAAGAAAUAACGUUAUUGCACUUAUUAUUUU ((.(((....((((((((((...(((((((.(((.......))).))))))).((((((.((((((((((....(((((((........(..((.....))..)........)))))))............((((.((...))...)))).......))))))).))).....(((((((....))))))).....................))))))..............))))))))))(((((.(((((....(((((((((((..(((......))).(....)...........).)))))))))).........(((((..(.(((((.....))))).)...)))))..))))).)))))....))).....))............ (-69.09) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 340 295 94 52 pint:PI2033|5end_hypothetical_protein_1987068..1987298_POSITIVE 294 320 276 89 36 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 288 48 2 155 111 pint:PI0597|5end_hypothetical_protein_546626..546856_POSITIVE 287 298 252 146 88 pint:PI1501|5end_GTP-binding_elongation_factor_family_protein_TypA/BipA_1421424..1421654_POSITIVE 287 150 107 188 152 pint:PI0269|5end_conserved_hypothetical_protein_259835..260065_POSITIVE 283 323 284 52 8 pint:PI0787|5end_glycosyltransferase_724136..724366_POSITIVE 283 89 41 103 53 pint:PI0763|5end_thymidine_kinase_697317..697547_POSITIVE 281 335 291 75 26 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 277 144 106 231 202 pint:PI0400|5end_conserved_hypothetical_protein_364429..364659_POSITIVE 276 75 31 121 84 pint:PI2044|5end_hypothetical_protein_1996999..1997229_POSITIVE 271 320 273 99 46 pint:PI0670|5end_hypothetical_protein_611000..611230_POSITIVE 262 300 265 230 193 pint:PI0882|5end_conserved_hypothetical_protein;_possible_para-aminobenzoate_synthase_component_I_815545..815775_POSITIVE 262 64 22 188 143 pint:PI0294|5end_conserved_hypothetical_protein_276223..276453_POSITIVE 261 270 227 69 24 pint:PI1767|5end_fructokinase,_pfkB_family_1694285..1694515_POSITIVE 261 77 31 171 123 pint:PI0218|5end_conserved_hypothetical_protein_210770..211000_POSITIVE 260 146 111 223 190 pint:PI1870|5end_hypothetical_protein_1815302..1815532_POSITIVE 260 146 111 52 19 pint:PI1869|5end_hypothetical_protein_1815131..1815361_POSITIVE 258 280 233 162 110 pint:PI0743|5end_hypothetical_protein_672728..672958_POSITIVE 256 56 13 199 146 pint:PI0999|5end_hypothetical_protein_922376..922606_POSITIVE 254 128 90 161 113 pint:PI1652|5end_hypothetical_protein_1564655..1564885_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 344 259 213 149 104 pint:PI0798|3end_malonyl_CoA-acyl_carrier_protein_transacylase_736975..737125_POSITIVE 309 340 295 67 25 pint:PI2032|3end_RNA_polymerase_sigma_factor,_ECF_subfamily_1987121..1987271_POSITIVE 288 48 2 57 13 pint:PI0596|3end_hypothetical_protein_546608..546758_POSITIVE 287 150 107 63 27 pint:PI0268|3end_lysozyme-related_protein_259790..259940_POSITIVE 281 335 291 151 102 pint:PI1541|3end_hypothetical_protein_1461884..1462034_POSITIVE 278 80 37 69 28 pint:PI1681|3end_Mg2+/Co2+_transporter_1598129..1598279_POSITIVE 261 270 227 54 9 pint:PI1766|3end_sugar_transporter,_possible_glucose/galactose_transporter_1694350..1694500_POSITIVE 261 77 31 115 67 pint:PI0217|3end_conserved_hypothetical_protein;_possible_lipoprotein_210794..210944_POSITIVE 260 307 265 137 92 pint:PI0378|3end_conserved_hypothetical_protein;_possible_helicase_349539..349689_POSITIVE 258 280 233 103 51 pint:PI0742|3end_hypothetical_protein_672749..672899_POSITIVE 254 49 7 150 107 pint:PI1432|3end_DNA_methylase_1353771..1353921_POSITIVE 248 280 239 43 3 pint:PI1944|3end_hypothetical_protein_1901990..1902140_POSITIVE 247 144 102 100 62 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 243 334 291 83 35 pint:PI1950|3end_iron-regulated_membrane_permease_1905888..1906038_POSITIVE 242 77 31 111 53 pint:PI1781|3end_membrane-associated_zinc_metalloprotease_1714551..1714701_POSITIVE 240 293 251 55 25 pint:PI0635|3end_dephospho-CoA_kinase_581690..581840_POSITIVE 239 43 4 145 116 pint:PI1744|3end_chromosome_partitioning_protein_1666620..1666770_POSITIVE 239 320 276 88 44 pint:PI0890|3end_ribose_5-phosphate_isomerase_B_820931..821081_POSITIVE 239 139 102 37 6 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 239 287 244 130 97 pint:PI0007|3end_hypothetical_protein_7300..7450_POSITIVE