Origin IGS: tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaggaatatattaccttcctttggtactttaaaactaccctcaaggttatatgtttatttctttgctgtctgaaatttaattagtaaatttgcacgcattaaaagcagtagaca Mask Tandem Repeat Region ================================================ tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Find is-nt database================================================ Query_seq: PI0829:PI0830|PI0829:PI0830:nucleotidyl transferase family; possible mannose-1-phosphate guanyltransferase:conserved hypothetical protein:->->:766600..766715 116 tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0829:PI0830|PI0829:PI0830:nucleotidyl transferase family; possible mannose-1-phosphate guanyltransferase:conserved hypothetical protein:->->:766600..766715 116 tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0829:PI0830|PI0829:PI0830:nucleotidyl transferase family; possible mannose-1-phosphate guanyltransferase:conserved hypothetical protein:->->:766600..766715 116 tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Predict ORF larger than 30AA ================================================ tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0829:PI0830|PI0829:PI0830:nucleotidyl transferase family; possible mannose-1-phosphate guanyltransferase:conserved hypothetical protein:->->:766600..766715 116 tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: pint_PI0829_PI0830|nucleotidyl transferase family; possible mannose-1-phosphate guanyltransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[766600,766715]|116 HSP 1 e-value: 8.0E-60 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta tgtctactgcttttaatgcgtgcaaatttactaattaaatttcagacagcaaagaaataaacatataaccttgagggtagttttaaagtaccaaaggaaggtaatatattcctcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUCUACUGCUUUUAAUGCGUGCAAAUUUACUAAUUAAAUUUCAGACAGCAAAGAAAUAAACAUAUAACCUUGAGGGUAGUUUUAAAGUACCAAAGGAAGGUAAUAUAUUCCUCUA (((((...((.......))....(((((((.....))))))).)))))..............((((.(((((...((((.(.....).)))).....))))).))))......... (-16.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAGGAAUAUAUUACCUUCCUUUGGUACUUUAAAACUACCCUCAAGGUUAUAUGUUUAUUUCUUUGCUGUCUGAAAUUUAAUUAGUAAAUUUGCACGCAUUAAAAGCAGUAGACA ((((((((.........)))))))).............(((.....)))....((((((((.((((..(((.(((((((((.....))))))).)).)))...)))).)))))))) (-20.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 26 1 117 86 pint:PI1154|5end_conserved_hypothetical_protein;_possible_periplasmic_solute-binding_protein_1078973..1079203_POSITIVE 246 101 61 150 113 pint:PI2112|5end_phosphopantetheine_adenylyltransferase_2063194..2063424_POSITIVE 235 31 7 79 56 pint:PI1679|5end_hypothetical_protein_1594744..1594974_POSITIVE 227 99 63 76 31 pint:PI2039|5end_hypothetical_protein_1992633..1992863_POSITIVE 224 35 4 174 146 pint:PI0333|5end_hypothetical_protein_313478..313708_POSITIVE 211 24 2 157 129 pint:PI1916|5end_conserved_hypothetical_protein_1860211..1860441_POSITIVE 211 84 44 146 102 pint:PI0719|5end_conserved_hypothetical_protein_653043..653273_POSITIVE 208 50 8 80 38 pint:PI1818|5end_hypothetical_protein_1756891..1757121_POSITIVE 204 32 1 160 127 pint:PI1364|5end_dipeptidyl_peptidase_IV_1281862..1282092_POSITIVE 204 97 69 44 19 pint:PI1029|5end_seryl-tRNA_synthetase_945938..946168_POSITIVE 202 101 61 200 160 pint:PI0303|5end_hypothetical_protein_286946..287176_POSITIVE 200 102 69 57 19 pint:PI0973|5end_glycine_dehydrogenase_[decarboxylating]_subunit_1_899483..899713_POSITIVE 198 50 7 68 22 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 197 24 3 45 24 pint:PI0146|5end_transcription_elongation_factor_140338..140568_POSITIVE 195 106 62 89 38 pint:PI2012|5end_hypothetical_protein_1970807..1971037_POSITIVE 194 103 67 206 153 pint:PI0215|5end_hypothetical_protein_208823..209053_POSITIVE 190 50 9 231 187 pint:PI2003|5end_hypothetical_protein_1960823..1961053_POSITIVE 190 50 9 70 26 pint:PI2002|5end_hypothetical_protein_1960662..1960892_POSITIVE 190 50 9 97 53 pint:PI1604|5end_hypothetical_protein_1534017..1534247_POSITIVE 190 50 9 58 14 pint:PI1129|5end_hypothetical_protein_1055377..1055607_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 246 101 61 134 97 pint:PI2111|3end_conserved_hypothetical_protein_2063258..2063408_POSITIVE 211 84 44 143 99 pint:PI0717|3end_conserved_hypothetical_protein_653120..653270_POSITIVE 204 32 1 140 107 pint:PI1363|3end_hypothetical_protein_1281922..1282072_POSITIVE 200 102 69 85 47 pint:PI0974|3end_hypothetical_protein_899591..899741_POSITIVE 198 50 7 122 76 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 197 24 5 39 16 pint:PI1710|3end_ABC-type_permease_component_1627356..1627506_POSITIVE 195 108 62 79 26 pint:PI1494|3end_conserved_hypothetical_protein_1413968..1414118_POSITIVE 191 102 62 50 9 pint:PI0363|3end_hypothetical_protein_333760..333910_POSITIVE 190 32 5 31 2 pint:PI1362|3end_60Kd_inner_membrane_protein_1281813..1281963_POSITIVE 190 50 9 53 9 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 190 50 9 112 68 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 190 86 44 127 83 pint:PI0163|3end_conserved_hypothetical_protein_158410..158560_POSITIVE 188 103 69 91 63 pint:PI2044|3end_hypothetical_protein_1997351..1997501_POSITIVE 187 100 75 40 15 pint:PI1377|3end_cAMP-binding_domain_protein_(cyclic_nucleotide-binding_domain)_1299959..1300109_POSITIVE 187 85 41 91 50 pint:PI1224|3end_sensor_histidine_kinase_and_response_regulator_1147557..1147707_POSITIVE 187 101 63 140 105 pint:PI0898|3end_conserved_hypothetical_protein_828730..828880_POSITIVE 186 31 7 147 124 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 184 104 69 101 61 pint:PI2169|3end_hypothetical_protein_2112325..2112475_POSITIVE 183 97 68 48 18 pint:PI1927|3end_hypothetical_protein_1872046..1872196_POSITIVE 182 45 3 63 11 pint:PI1299|3end_glycosyltransferase_1213251..1213401_POSITIVE