Origin IGS: tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattctcgataagtgcttttatagctattatttaactgaaaagcacttatcgataaagaaaactttttcttttattcaactttttattagcatttaaagtgctaatatttaatcttatacacataaaactcatgaattaaaggaagaaaataacacaaaaaagtaggtgaaatatttggaggtatgcaaaaaactcactaactttgccatagtttttttagtggttaatttagttttagtattggaaccttggttgatgtgatatcagtcaaggcttttttatgctcgctatttttcttcattttgtaaagaacagcaataccaagccgttcttacgttatccttgttcctacatataaacaatgtactatcttcatagcaaatctatcaaagtataaattcaagcaattgatttatatcataaaacaacgacaaaatggtgtaaaagttacaaaataaactattcgatgcattaataataaaaaactttcgtatctttgcatcgtaagattaaggataataacaaacaaagctgtcggaagaaaacattgctggcagctttttaaaaagaaaggattaattatgacaatagcattattagcaacaacagcattcgtagtatcaactcttgtggctgcattcgataataagtattaagtgcaaaacaaggtatcgctaagggtctatatccctcacaacgcaaacagttaaaggcatagcaacaagcgatacaacgatactaaaacgaaagaaccctaaaacattcttcgcaatagcaaagacccgttttagggttctttcttatttatatatattgttgaatcattcttttggtaggcta Mask Tandem Repeat Region ================================================ tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Find is-nt database================================================ Query_seq: PI0803:PI0804|PI0803:PI0804:DNA-damage-inducible protein F:conserved hypothetical protein; possible membrane protein:->->:741232..742073 842 tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0803:PI0804|PI0803:PI0804:DNA-damage-inducible protein F:conserved hypothetical protein; possible membrane protein:->->:741232..742073 842 tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0803:PI0804|PI0803:PI0804:DNA-damage-inducible protein F:conserved hypothetical protein; possible membrane protein:->->:741232..742073 842 tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 68 End: 163 ................................................................... M A K N V L G F F R F S I V V S L V A M P L T V C V V R D I D P ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 49 Strand: + Start: 112 End: 258 ............................................................................................................... M A C C Y A F N C L R C E G Y R P L A I P C F A L N T Y Y R M Q P Q E L I L R M L L L L I M L L S ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 58 Strand: + Start: 252 End: 425 ........................................................................................................................................................................................................................................................... M V I I N P F F L K S C Q Q C F L P T A L F V I I L N L T M Q R Y E S F L L L M H R I V Y F V T F T P F C R C F M I ................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 35 Strand: + Start: 347 End: 451 .......................................................................................................................................................................................................................................................................................................................................................... M R K F F I I N A S N S L F C N F Y T I L S L F Y D I N Q L L E F I L ....................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 458 End: 550 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M C Y E D S T L F I C R N K D N V R T A W Y C C S L Q N E E K .................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 522 End: 614 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M A V L Y K M K K N S E H K K A L T D I T S T K V P I L K L N .................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 584 End: 709 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M N Q G S N T K T K L T T K K T M A K L V S F L H T S K Y F T Y F F V L F S S F N S ..................................................................................................................................... Protein_Len: 31 Strand: - Start: 748 End: 840 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I S I F L Q I F S F T K K I S L H K E T L Y Y S Y F C K D L M .. Protein_Len: 31 Strand: - Start: 583 End: 675 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L W P E L V L V L N V V L F V I A F N T L K K C V E L Y K M ....................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 486 End: 578 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I Y S C P Y R L F P K T N S N K V F H L F I A L M F F G Q S M ........................................................................................................................................................................................................................................................................ Protein_Len: 46 Strand: - Start: 386 End: 523 ................................................................................................................................................................................................................................................................................................................................................................................................. K T V K V G N Q R Q K I I Y I L Q K F K Y K S L N A I F I T C Q K Y T P V L I V Y S R S P M ............................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 188 End: 379 ........................................................................................................................................................................................... F A A V L T S V V F A T T A L L A I T M I L G K K K F L Q W C H K R G V A K N T I I R L R V I C L Y S L K K N N I C R M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 1 End: 156 L R G F S H N L L I Y L Y S L V R F R T K A I A F F T K P N K R K L I T T D S T A I G K V T Q T T L S M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 395 End: 424 ..........................................................................................................................................................................................................................................................................................................................................................................................................TTTTACACCATTTTGTCGTTGTTTTATGAT.................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -14.8 Tail_Score: -4.39889 Start: 40 End: 96 Full_Region: CAATATATATAAATA AGAAAGAACCCTAAAACGGGTCTTTGC TATT GCGAAGAATGTTTTAGGGTTCTTTCG TTTTAGTATCGTTGT .......................................AGAAAGAACCCTAAAACGGGTCTTTGCTATTGCGAAGAATGTTTTAGGGTTCTTTCG.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -18.3 Tail_Score: -3.22849 Start: 41 End: 95 Full_Region: AATATATATAAATAA GAAAGAACCCTAAAACGGGTCTTTGC TATT GCGAAGAATGTTTTAGGGTTCTTTC GTTTTAGTATCGTTG ........................................GAAAGAACCCTAAAACGGGTCTTTGCTATTGCGAAGAATGTTTTAGGGTTCTTTC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -13.9 Tail_Score: -4.21184 Start: 41 End: 95 Full_Region: caacgatactaaaac gaaagaaccctaaaacattcttcgc aata gcaaagacccgttttagggttctttc ttatttatatatatt ........................................gaaagaaccctaaaacattcttcgcaatagcaaagacccgttttagggttctttc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 89 HP_score: -8.9 Tail_Score: -4.65458 Start: 45 End: 91 Full_Region: gatactaaaacgaaa gaaccctaaaacattcttcgc aata gcaaagacccgttttagggttc tttcttatttatata ............................................gaaccctaaaacattcttcgcaatagcaaagacccgttttagggttc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 52 HP_score: -3.7 Tail_Score: -3.55444 Start: 510 End: 531 Full_Region: ACAAGGATAACGTAA GAACGGC TTGGTATT GCTGTTC TTTACAAAATGAAGA .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAACGGCTTGGTATTGCTGTTC....................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 79 HP_score: -5.3 Tail_Score: -5.31877 Start: 567 End: 595 Full_Region: agttttagtattgga accttggttgatg tga tatcagtcaaggc ttttttatgctcgct ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................accttggttgatgtgatatcagtcaaggc....................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 80 HP_score: -8.8 Tail_Score: -3.78042 Start: 568 End: 594 Full_Region: gttttagtattggaa ccttggttgatg tga tatcagtcaagg cttttttatgctcgc .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ccttggttgatgtgatatcagtcaagg........................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 82 HP_score: -8.6 Tail_Score: -3.97088 Start: 735 End: 757 Full_Region: ttttattcaactttt tattagcatt taa agtgctaata tttaatcttatacac ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tattagcatttaaagtgctaata..................................................................................... Find igs database================================================ Query_seq: PI0803:PI0804|PI0803:PI0804:DNA-damage-inducible protein F:conserved hypothetical protein; possible membrane protein:->->:741232..742073 842 tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Intra-Species Hit: Count: 1 Min: 1 Max: 842 Len: 842 Subject: pint_PI0803_PI0804|DNA-damage-inducible protein F:conserved hypothetical protein; possible membrane protein|POSITIVE:POSITIVE|[741232,742073]|842 HSP 1 e-value: 0.0 bit: 1628.0 Len: 842 Query Start:1 Query End:842 Subject Strand: POSITIVE Subject Start: 1 Subject End: 842 tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactnnnnnnnctatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta tagcctaccaaaagaatgattcaacaatatatataaataagaaagaaccctaaaacgggtctttgctattgcgaagaatgttttagggttctttcgttttagtatcgttgtatcgcttgttgctatgcctttaactgtttgcgttgtgagggatatagacccttagcgataccttgttttgcacttaatacttattatcgaatgcagccacaagagttgatactacgaatgctgttgttgctaataatgctattgtcataattaatcctttctttttaaaaagctgccagcaatgttttcttccgacagctttgtttgttattatccttaatcttacgatgcaaagatacgaaagttttttattattaatgcatcgaatagtttattttgtaacttttacaccattttgtcgttgttttatgatataaatcaattgcttgaatttatactttgatagatttgctatgaagatagtacattgtttatatgtaggaacaaggataacgtaagaacggcttggtattgctgttctttacaaaatgaagaaaaatagcgagcataaaaaagccttgactgatatcacatcaaccaaggttccaatactaaaactaaattaaccactaaaaaaactatggcaaagttagtgagttttttgcatacctccaaatatttcacctacttttttgtgttattttcttcctttaattcatgagttttatgtgtataagattaaatattagcactttaaatgctaataaaaagttgaataaaagaaaaagttttctttatcgataagtgcttttcagttaaataatagctataaaagcacttatcgagaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCUACCAAAAGAAUGAUUCAACAAUAUAUAUAAAUAAGAAAGAACCCUAAAACGGGUCUUUGCUAUUGCGAAGAAUGUUUUAGGGUUCUUUCGUUUUAGUAUCGUUGUAUCGCUUGUUGCUAUGCCUUUAACUGUUUGCGUUGUGAGGGAUAUAGACCCUUAGCGAUACCUUGUUUUGCACUUAAUACUUAUUAUCGAAUGCAGCCACAAGAGUUGAUACUACGAAUGCUGUUGUUGCUAAUAAUGCUAUUGUCAUAAUUAAUCCUUUCUUUUUAAAAAGCUGCCAGCAAUGUUUUCUUCCGACAGCUUUGUUUGUUAUUAUCCUUAAUCUUACGAUGCAAAGAUACGAAAGUUUUUUAUUAUUAAUGCAUCGAAUAGUUUAUUUUGUAACUUUUACACCAUUUUGUCGUUGUUUUAUGAUAUAAAUCAAUUGCUUGAAUUUAUACUUUGAUAGAUUUGCUAUGAAGAUAGUACAUUGUUUAUAUGUAGGAACAAGGAUAACGUAAGAACGGCUUGGUAUUGCUGUUCUUUACAAAAUGAAGAAAAAUAGCGAGCAUAAAAAAGCCUUGACUGAUAUCACAUCAACCAAGGUUCCAAUACUAAAACUAAAUUAACCACUAAAAAAACUAUGGCAAAGUUAGUGAGUUUUUUGCAUACCUCCAAAUAUUUCACCUACUUUUUUGUGUUAUUUUCUUCCUUUAAUUCAUGAGUUUUAUGUGUAUAAGAUUAAAUAUUAGCACUUUAAAUGCUAAUAAAAAGUUGAAUAAAAGAAAAAGUUUUCUUUAUCGAUAAGUGCUUUUCAGUUAAAUAAUAGCUAUAAAAGCACUUAUCGAGAAUUA ..................(((((((..((((((((.....(((((((((((((((((..(((((((....))))))).)))))))))))))))))....((((((((..((((((((.....(((((((((((..((....))...)))))).)))))......))))))))(((((.(((((.(..(((.....)))..).)))))..)))))...)))))))).....(((((((..(..((((.((((...((.....((((..........))))...))....)))).))))..)...)))))))..(((((((((((.............(((((((((((.((....)).))))........))))))).....(((((((((((..............(((((.(((((((...(((((((.((((.....((((((((......))))))))(((((....)))))..)))))))))))...))))))).)))))...(((((((((........))))))))))))))))))))....)))))))))))......(((((((..((((.....))))..))))))).......(((((((.((((((.....((((.(((.(..(.(((((..(((((...((((........))))...)))))..))))).)..)))).))))......))))))....)))))))))))))))........((((((((.......))))))))....))))))).(((((((....))))))).(((((((((((((((.(((((.....)))))..)))))))))))))))...... (-221.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCUCGAUAAGUGCUUUUAUAGCUAUUAUUUAACUGAAAAGCACUUAUCGAUAAAGAAAACUUUUUCUUUUAUUCAACUUUUUAUUAGCAUUUAAAGUGCUAAUAUUUAAUCUUAUACACAUAAAACUCAUGAAUUAAAGGAAGAAAAUAACACAAAAAAGUAGGUGAAAUAUUUGGAGGUAUGCAAAAAACUCACUAACUUUGCCAUAGUUUUUUUAGUGGUUAAUUUAGUUUUAGUAUUGGAACCUUGGUUGAUGUGAUAUCAGUCAAGGCUUUUUUAUGCUCGCUAUUUUUCUUCAUUUUGUAAAGAACAGCAAUACCAAGCCGUUCUUACGUUAUCCUUGUUCCUACAUAUAAACAAUGUACUAUCUUCAUAGCAAAUCUAUCAAAGUAUAAAUUCAAGCAAUUGAUUUAUAUCAUAAAACAACGACAAAAUGGUGUAAAAGUUACAAAAUAAACUAUUCGAUGCAUUAAUAAUAAAAAACUUUCGUAUCUUUGCAUCGUAAGAUUAAGGAUAAUAACAAACAAAGCUGUCGGAAGAAAACAUUGCUGGCAGCUUUUUAAAAAGAAAGGAUUAAUUAUGACAAUAGCAUUAUUAGCAACAACAGCAUUCGUAGUAUCAACUCUUGUGGCUGCAUUCGAUAAUAAGUAUUAAGUGCAAAACAAGGUAUCGCUAAGGGUCUAUAUCCCUCACAACGCAAACAGUUAAAGGCAUAGCAACAAGCGAUACAACGAUACUAAAACGAAAGAACCCUAAAACAUUCUUCGCAAUAGCAAAGACCCGUUUUAGGGUUCUUUCUUAUUUAUAUAUAUUGUUGAAUCAUUCUUUUGGUAGGCUA ......(((((((((((((((.(((.((.....)).))))))))))))))))))..(((((((((((((((((((((((......(((((((((.....))))))))).......((((....))))......)))).))))))))))............(((((.(((((....((((.(....).))))....))))).))))).....))))))))).((((((.((((......(((((.(((((((((..(((((...)))))..))))).))))..))))).(..(((((((.(((((((((.((((((.((........)).))))))..(((((.........(((((.......)))))........)))))............((((((((.(((....)))))))))))............))))))))).).))))))..).)))).))))))..((((((((.(((((.((...(((((.((((((((.(((....))))))))))).........(((((((((((((.......)).))))))))))).......)))))..)).)))))...))).)))))..((((..((((((......(((((((....(((((...((((((..(((.....)))..))))))..)))))((((((((.((((.......)))).............((((......))))....))))))))...)))))))....((((((((((((((((..((((.((....)).))))...)))))))))))))))).............)))))).((((......))))..)))) (-213.13) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 341 349 303 96 37 pint:PI0935|5end_collagenase,_protease_866297..866527_POSITIVE 328 97 55 201 158 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 326 160 115 96 49 pint:PI0328|5end_conserved_hypothetical_protein_309476..309706_POSITIVE 311 534 491 46 3 pint:PI1886|5end_cell_division_protein_1827633..1827863_POSITIVE 310 320 271 98 52 pint:PI0824|5end_large_conductance_mechanosensitive_channel_protein_763337..763567_POSITIVE 303 148 101 55 19 pint:PI1935|5end_possible__internalin-related_protein_1883324..1883554_POSITIVE 301 129 89 82 36 pint:PI1629|5end_hypothetical_protein_1551707..1551937_POSITIVE 301 97 53 79 29 pint:PI1102|5end_conserved_hypothetical_protein_1025316..1025546_POSITIVE 299 156 115 193 151 pint:PI0057|5end_conserved_hypothetical_protein_55959..56189_POSITIVE 298 152 115 190 144 pint:PI0086|5end_possible_uroporphyrinogen-III_synthase_84944..85174_POSITIVE 297 125 84 80 30 pint:PI1328|5end_conserved_hypothetical_protein_1246197..1246427_POSITIVE 296 133 91 168 129 pint:PI0529|5end_hypothetical_protein_483026..483256_POSITIVE 295 143 101 47 6 pint:PI0301|5end_probable_transposase_285672..285902_POSITIVE 292 156 113 215 173 pint:PI1295|5end_hypothetical_protein_1208636..1208866_POSITIVE 292 156 113 69 27 pint:PI1294|5end_hypothetical_protein_1208490..1208720_POSITIVE 291 148 105 129 73 pint:PI1747|5end_membrane-bound_lytic_murein_transglycosylase_D_1667483..1667713_POSITIVE 291 96 55 131 90 pint:PI1711|5end_ABC_transporter,_ATP-binding_protein_1627597..1627827_POSITIVE 290 147 102 216 164 pint:PI1660|5end_hypothetical_protein_1574659..1574889_POSITIVE 290 328 284 74 24 pint:PI0530|5end_possible_membrane-fusion_protein_483772..484002_POSITIVE 286 169 122 231 178 pint:PI1532|5end_uridylate_kinase_1451644..1451874_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 404 90 46 45 1 pint:PI0803|3end_DNA-damage-inducible_protein_F_741171..741321_POSITIVE 305 177 135 137 87 pint:PI1267|3end_probable_integrase_1186475..1186625_POSITIVE 302 146 105 103 60 pint:PI2166|3end_hypothetical_protein_2112073..2112223_POSITIVE 301 129 89 77 31 pint:PI1628|3end_hypothetical_protein_1551782..1551932_POSITIVE 299 156 115 61 19 pint:PI0056|3end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_55907..56057_POSITIVE 298 152 115 126 80 pint:PI0085|3end_RNase_P_protein_component_84960..85110_POSITIVE 297 125 84 69 19 pint:PI1327|3end_conserved_hypothetical_protein_1246266..1246416_POSITIVE 295 518 486 151 117 pint:PI1224|3end_sensor_histidine_kinase_and_response_regulator_1147557..1147707_POSITIVE 295 143 101 56 15 pint:PI0300|3end_transposase_285761..285911_POSITIVE 295 559 512 70 27 pint:PI0017|3end_thiol-disulfide_isomerase;_thioredoxin_22835..22985_POSITIVE 294 240 199 50 3 pint:PI0245|3end_possible_mobilization_protein_244258..244408_POSITIVE 292 129 87 133 93 pint:PI0591|3end_adenylosuccinate_lyase_540018..540168_POSITIVE 288 167 122 139 95 pint:PI0126|3end_conserved_hypothetical_protein_118589..118739_POSITIVE 284 148 103 78 35 pint:PI1768|3end_conserved_hypothetical_protein_1696639..1696789_POSITIVE 281 327 283 140 93 pint:PI1492|3end_triosephosphate_isomerase_1412310..1412460_POSITIVE 279 146 102 151 107 pint:PI0620|3end_hypothetical_protein_570630..570780_POSITIVE 271 146 104 75 42 pint:PI2122|3end_conserved_hypothetical_protein_2070480..2070630_POSITIVE 269 225 181 67 22 pint:PI1875|3end_hypothetical_protein_1819106..1819256_POSITIVE 267 324 285 139 91 pint:PI1229|3end_conserved_hypothetical_protein_1151669..1151819_POSITIVE 266 147 104 114 67 pint:PI1143|3end_hypothetical_protein_1070504..1070654_POSITIVE