Origin IGS: taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacactctggtaactgaacttctgcctaagtctgcatatttctgccctttacaaatagagcagtattatgcaaatcagagcatttcctcctcattatcagttagtttacactaatttcgatatttctccattttcgttgattagttacagtgcaataaatatttctttacgtgtagataaatattta Mask Tandem Repeat Region ================================================ taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Find is-nt database================================================ Query_seq: PI0725:PI0726|PI0725:PI0726:hypothetical protein:hypothetical protein:->->:658994..659181 188 taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0725:PI0726|PI0725:PI0726:hypothetical protein:hypothetical protein:->->:658994..659181 188 taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0725:PI0726|PI0725:PI0726:hypothetical protein:hypothetical protein:->->:658994..659181 188 taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 77 End: 181 ............................................................................ H L S V S L S S S I S Q N A Y Y Q E I Q L P C F Y A S K P L L E T V M ....... Protein_Len: 41 Strand: - Start: 1 End: 123 L Y K D V R L S I N I A S Y S I L S F P S I D F N T Y V L Q Y H P P F A R I Q M M ................................................................. taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0725:PI0726|PI0725:PI0726:hypothetical protein:hypothetical protein:->->:658994..659181 188 taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Intra-Species Hit: Count: 2 Min: 1 Max: 188 Len: 188 Subject: pint_PI0725_PI0726|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[658994,659181]|188 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 188 Query Start:1 Query End:188 Subject Strand: POSITIVE Subject Start: 1 Subject End: 188 taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta taaatatttatctacacgtaaagaaatatttattgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttaccagagtgtta Subject: pint_PI1560_PI1561|integrase:conserved hypothetical protein; possible lipoprotein|POSITIVE:POSITIVE|[1478326,1478856]|531 HSP 1 e-value: 7.0E-12 bit: 71.9 Len: 148 Query Start:33 Query End:180 Subject Strand: NEGATIVE Subject Start: 168 Subject End: 315 ................................ttgcactgtaactaatcaacgaaaatggagaaatatcgaaattagtgtaaactaactgataatgaggaggaaatgctctgatttgcataatactgctctatttgtaaagggcagaaatatgcagacttaggcagaagttcagttacca........ ................................ttgcacggtaactaatcacagaaaatgaagcaaaatcgaagttggcgaaaactaactgataatgtgggagaaacggaacgatttgcatagaagtactcttttcaaaaagggcaggaatatgcagatttaggcagaagttcagttacca........ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAUUUAUCUACACGUAAAGAAAUAUUUAUUGCACUGUAACUAAUCAACGAAAAUGGAGAAAUAUCGAAAUUAGUGUAAACUAACUGAUAAUGAGGAGGAAAUGCUCUGAUUUGCAUAAUACUGCUCUAUUUGUAAAGGGCAGAAAUAUGCAGACUUAGGCAGAAGUUCAGUUACCAGAGUGUUA (((((((((...((....))...)))))))))..((((((((((.......................(((..((((((........))))))..)))....(((.(((.((((((((((((...(((((((........)))))))...)))))))).)))))))....))).)))))...))))).. (-43.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACUCUGGUAACUGAACUUCUGCCUAAGUCUGCAUAUUUCUGCCCUUUACAAAUAGAGCAGUAUUAUGCAAAUCAGAGCAUUUCCUCCUCAUUAUCAGUUAGUUUACACUAAUUUCGAUAUUUCUCCAUUUUCGUUGAUUAGUUACAGUGCAAUAAAUAUUUCUUUACGUGUAGAUAAAUAUUUA ...((((...((((((((.....(((((..((.((((((...((((.((........)).))))...)))))).)).)).)))............(((((((((((....)))))))..))))..................))))))))))))...(((((((((.((((....)))).))))))))) (-37.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 797 170 121 141 92 pint:PI0406|5end_hypothetical_protein_372734..372964_POSITIVE 346 48 1 139 92 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 345 42 2 137 96 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 298 43 1 172 130 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 290 43 2 116 75 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 284 177 142 128 99 pint:PI1150|5end_30S_ribosomal_protein_S6_1075683..1075913_POSITIVE 278 42 6 130 94 pint:PI0983|5end_hypothetical_protein_909750..909980_POSITIVE 273 128 86 227 194 pint:PI2167|5end_hypothetical_protein_2111590..2111820_POSITIVE 268 167 125 80 35 pint:PI0265|5end_conjugative_transposon_protein,_TraO_257296..257526_POSITIVE 266 180 140 57 9 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 266 178 133 84 33 pint:PI0861|5end_glucosamine--fructose-6-phosphate_aminotransferase_(glutamine-fructose-6-phosphate_transaminase_(isomerizing))_796346..796576_POSITIVE 263 170 138 125 96 pint:PI1979|5end_conserved_hypothetical_protein_1935472..1935702_POSITIVE 260 172 135 226 182 pint:PI0086|5end_possible_uroporphyrinogen-III_synthase_84944..85174_POSITIVE 256 147 106 223 182 pint:PI1928|5end_conserved_hypothetical_protein_1871996..1872226_POSITIVE 256 145 101 141 96 pint:PI0727|5end_integrase_659261..659491_POSITIVE 256 170 127 53 12 pint:PI0640|5end_conserved_hypothetical_protein_583289..583519_POSITIVE 255 170 142 36 1 pint:PI1677|5end_cell_division_protein_FtsH_1591766..1591996_POSITIVE 255 120 87 91 57 pint:PI0396|5end_possible_tetracycline_resistance_element_mobilization_regulatory_protein,_C-terminal_fragment_361993..362223_POSITIVE 250 169 125 55 8 pint:PI0224|5end_conserved_hypothetical_protein_220541..220771_POSITIVE 247 43 8 116 81 pint:PI0648|5end_hypothetical_protein_589448..589678_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 797 170 121 72 23 pint:PI0405|3end_hypothetical_protein_372745..372895_POSITIVE 284 177 142 122 93 pint:PI1149|3end_30S_ribosomal_protein_S18_1075757..1075907_POSITIVE 283 146 101 140 96 pint:PI1430|3end_subtilisin-like_serine_proteinase_1351774..1351924_POSITIVE 268 167 125 75 30 pint:PI0264|3end_transfer_region-related_protein,_TraN_257371..257521_POSITIVE 266 180 140 133 85 pint:PI1541|3end_hypothetical_protein_1461884..1462034_POSITIVE 263 170 138 107 78 pint:PI1978|3end_hypothetical_protein_1935534..1935684_POSITIVE 263 168 124 70 27 pint:PI0156|3end_conserved_hypothetical_protein_150913..151063_POSITIVE 250 123 93 73 35 pint:PI1043|3end_conserved_hypothetical_protein_961597..961747_POSITIVE 246 43 8 50 15 pint:PI1314|3end_hypothetical_protein_1231423..1231573_POSITIVE 244 169 124 63 22 pint:PI0681|3end_hypothetical_protein_619256..619406_POSITIVE 240 170 121 85 42 pint:PI0738|3end_conserved_hypothetical_protein_671871..672021_POSITIVE 239 169 123 72 28 pint:PI0519|3end_5'-nucleotidase/2',3'-cyclic_phosphodiesterase_478809..478959_POSITIVE 238 118 74 70 25 pint:PI0890|3end_ribose_5-phosphate_isomerase_B_820931..821081_POSITIVE 237 105 69 86 50 pint:PI0065|3end_conserved_hypothetical_protein_65312..65462_POSITIVE 235 134 92 112 77 pint:PI0378|3end_conserved_hypothetical_protein;_possible_helicase_349539..349689_POSITIVE 234 159 111 79 35 pint:PI1973|3end_conserved_hypothetical_protein_1931171..1931321_POSITIVE 232 75 43 105 58 pint:PI1052|3end_fructose-1,6-bisphosphatase_972581..972731_POSITIVE 231 146 108 110 60 pint:PI2039|3end_hypothetical_protein_1992991..1993141_POSITIVE 230 178 140 56 20 pint:PI1676|3end_phosphatidate_cytidylyltransferase_1591773..1591923_POSITIVE 230 161 123 42 3 pint:PI0782|3end_beta-lactamase_induction_signal_transducer,_AmpG_protein_721927..722077_POSITIVE