Origin IGS: tgatggataaccttgcgcaccttatttatcgagcccatttggttcattggcacacgtacgagcctgctttgttccgaaatggcttgcattatgctttgtcgaatccaccatacagcatacgaaatgaacttaaatccccgtgtttcgtcgtatttcttagctgcttttatcagtcccagattcccttcgttgataaggtctggaagcgataaaccttgattttgatattgctttgctaccgagacgacaaatcgtaaattggcttttgtaagcctttcgagggcttttacatcgccttgccttatcttttgtgctaactcaacctcttcatctaagctcacgaggtcttcgcggcttatgtcttgaaggtatttttcgagtgctgtgctttctcgatttgtaatcgactgggtgattttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtattcctacatcgcttatgccctgaagcagttccttgctcatgatgtggcgcacagaaatgcggagcgaatcgttgcgttttagtggaaaaatggcaatgcgttgttgtatttcgcttgttgtaatcccgcgctttccagccaatcttccatcgtcgtttatgatgttgcaagttacggtatcttggtagctgcgacttgttagtttctttcttttcttgtgtattacggtgcgttcgacgatgaacgtaatgttcttgtacgggtagctttgcgatgcccttacgcccaagtccatactgtacattccttcccattggcgtggaatgtagaagacagctgtgtcgttgcgctcccacccattgataggcaaagctttatagctgtcgtaggtgcgggcatctttgcacgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggaaaggacattccttttcgcacatcatagcacttgcaatcgttgccattacagcgattgcgtgcaaagatgcccgcacctacgacagctataaagctttgcctatcaatgggtgggagcgcaacgacacagctgtcttctacattccacgccaatgggaaggaatgtacagtatggacttgggcgtaagggcatcgcaaagctacccgtacaagaacattacgttcatcgtcgaacgcaccgtaatacacaagaaaagaaagaaactaacaagtcgcagctaccaagataccgtaacttgcaacatcataaacgacgatggaagattggctggaaagcgcgggattacaacaagcgaaatacaacaacgcattgccatttttccactaaaacgcaacgattcgctccgcatttctgtgcgccacatcatgagcaaggaactgcttcagggcataagcgatgtaggaatacgaatcataaaacgcagataaacaaataataaaaggtagataaacaaaacgaaacagggcatctgaacagttgatactttcttttcaataagaccgtatttcccttttcaaatagcagaacgactgacaaaactcttctaaaaaggcgtaaaaaaacatatattattctgtttttccttgctcaactcactgtttttgtgtacatttgtccaatcttaatatcaatccacgatacagatgagacaactgaaaatcacccagtcgattacaaatcgagaaagcacagcactcgaaaaataccttcaagacataagccgcgaagacctcgtgagcttagatgaagaggttgagttagcacaaaagataaggcaaggcgatgtaaaagccctcgaaaggcttacaaaagccaatttacgatttgtcgtctcggtagcaaagcaatatcaaaatcaaggtttatcgcttccagaccttatcaacgaagggaatctgggactgataaaagcagctaagaaatacgacgaaacacggggatttaagttcatttcgtatgctgtatggtggattcgacaaagcataatgcaagccatttcggaacaaagcaggctcgtacgtgtgccaatgaaccaaatgggctcgataaataaggtgcgcaaggttatccatca Mask Tandem Repeat Region ================================================ tgatggataaccttgcgcaccttatttatcgagcccatttggttcattggcacacgtacgagcctgctttgttccgaaatggcttgcattatgctttgtcgaatccaccatacagcatacgaaatgaacttaaatccccgtgtttcgtcgtatttcttagctgcttttatcagtcccagattcccttcgttgataaggtctggaagcgataaaccttgattttgatattgctttgctaccgagacgacaaatcgtaaattggcttttgtaagcctttcgagggcttttacatcgccttgccttatcttttgtgctaactcaacctcttcatctaagctcacgaggtcttcgcggcttatgtcttgaaggtatttttcgagtgctgtgctttctcgatttgtaatcgactgggtgattttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngattcgtattcctacatcgcttatgccctgaagcagttccttgctcatgatgtggcgcacagaaatgcggagcgaatcgttgcgttttagtggaaaaatggcaatgcgttgttgtatttcgcttgttgtaatcccgcgctttccagccaatcttccatcgtcgtttatgatgttgcaagttacggtatcttggtagctgcgacttgttagtttctttcttttcttgtgtattacggtgcgttcgacgatgaacgtaatgttcttgtacgggtagctttgcgatgcccttacgcccaagtccatactgtacattccttcccattggcgtggaatgtagaagacagctgtgtcgttgcgctcccacccattgataggcaaagctttatagctgtcgtaggtgcgggcatctttgcacgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Find is-nt database================================================ Query_seq: PI0721:PI0723|PI0721:PI0723:hypothetical protein:conserved hypothetical protein:->->:655043..656182 1140 tgatggataaccttgcgcaccttatttatcgagcccatttggttcattggcacacgtacgagcctgctttgttccgaaatggcttgcattatgctttgtcgaatccaccatacagcatacgaaatgaacttaaatccccgtgtttcgtcgtatttcttagctgcttttatcagtcccagattcccttcgttgataaggtctggaagcgataaaccttgattttgatattgctttgctaccgagacgacaaatcgtaaattggcttttgtaagcctttcgagggcttttacatcgccttgccttatcttttgtgctaactcaacctcttcatctaagctcacgaggtcttcgcggcttatgtcttgaaggtatttttcgagtgctgtgctttctcgatttgtaatcgactgggtgattttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtattcctacatcgcttatgccctgaagcagttccttgctcatgatgtggcgcacagaaatgcggagcgaatcgttgcgttttagtggaaaaatggcaatgcgttgttgtatttcgcttgttgtaatcccgcgctttccagccaatcttccatcgtcgtttatgatgttgcaagttacggtatcttggtagctgcgacttgttagtttctttcttttcttgtgtattacggtgcgttcgacgatgaacgtaatgttcttgtacgggtagctttgcgatgcccttacgcccaagtccatactgtacattccttcccattggcgtggaatgtagaagacagctgtgtcgttgcgctcccacccattgataggcaaagctttatagctgtcgtaggtgcgggcatctttgcacgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0721:PI0723|PI0721:PI0723:hypothetical protein:conserved hypothetical protein:->->:655043..656182 1140 tgatggataaccttgcgcaccttatttatcgagcccatttggttcattggcacacgtacgagcctgctttgttccgaaatggcttgcattatgctttgtcgaatccaccatacagcatacgaaatgaacttaaatccccgtgtttcgtcgtatttcttagctgcttttatcagtcccagattcccttcgttgataaggtctggaagcgataaaccttgattttgatattgctttgctaccgagacgacaaatcgtaaattggcttttgtaagcctttcgagggcttttacatcgccttgccttatcttttgtgctaactcaacctcttcatctaagctcacgaggtcttcgcggcttatgtcttgaaggtatttttcgagtgctgtgctttctcgatttgtaatcgactgggtgattttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtattcctacatcgcttatgccctgaagcagttccttgctcatgatgtggcgcacagaaatgcggagcgaatcgttgcgttttagtggaaaaatggcaatgcgttgttgtatttcgcttgttgtaatcccgcgctttccagccaatcttccatcgtcgtttatgatgttgcaagttacggtatcttggtagctgcgacttgttagtttctttcttttcttgtgtattacggtgcgttcgacgatgaacgtaatgttcttgtacgggtagctttgcgatgcccttacgcccaagtccatactgtacattccttcccattggcgtggaatgtagaagacagctgtgtcgttgcgctcccacccattgataggcaaagctttatagctgtcgtaggtgcgggcatctttgcacgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 9 Max: 416 Len: 408 Subject: gi|89109528|ref|AP_003308.1| RNA polymerase, sigma S (sigma 38) factor [Escherichia coli W3110] HSP 1 e-value: 3.0E-25 bit: 113.0 Len: 393 Query Start:24 Query End:416 Subject Strand: null Subject Start: 7 Subject End: 137 ....................... I T Q S I T N R E S T A L E K Y L Q D I S R X X X X X X X X X X X L A Q K I R Q G D V K A L E R L T K A N L R F V V S V A K Q Y Q N Q G L S L P D L I N E G N L G L I K A A K K Y D E T R G F K F I S Y A V W W I R Q S I M Q A I S E Q S R L V R V P M N Q M G S I N .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ....................... L S Q G A T Q R V L D A T Q L Y L G E I G Y S P L L T A E E E V Y F A R R A L R G D V A S R R R M I E S N L R L V V K I A R R Y G N R G L A L L D L I E E G N L G L I R A V E K F D P E R G F R F S T Y A T W W I R Q T I E R A I M N Q T R T I R L P I H I V K E L N .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: gi|89109837|ref|AP_003617.1| RNA polymerase, sigma 70 (sigma D) factor [Escherichia coli W3110] HSP 1 e-value: 2.0E-23 bit: 106.0 Len: 279 Query Start:9 Query End:287 Subject Strand: null Subject Start: 374 Subject End: 466 ........ K A L E R L T K A N L R F V V S V A K Q Y Q N Q G L S L P D L I N E G N L G L I K A A K K Y D E T R G F K F I S Y A V W W I R Q S I M Q A I S E Q S R L V R V P M N Q M G S I N K V R K V ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ........ R A K K E M V E A N L R L V I S I A K K Y T N R G L Q F L D L I Q E G N I G L M K A V D K F E Y R R G Y K F S T Y A T W W I R Q A I T R S I A D Q A R T I R I P V H M I E T I N K L N R I ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: gi|89110551|ref|AP_004331.1| RNA polymerase, sigma 32 (sigma H) factor [Escherichia coli W3110] HSP 1 e-value: 5.0E-13 bit: 72.4 Len: 330 Query Start:51 Query End:380 Subject Strand: null Subject Start: 16 Subject End: 126 .................................................. L E K Y L Q D I S R X X X X X X X X X X X L A Q K I R - Q G D V K A L E R L T K A N L R F V V S V A K Q Y Q N Q G L S L P D L I N E G N L G L I K A A K K Y D E T R G F K F I S Y A V W W I R Q S I M Q A I S E Q S R L V R V ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ .................................................. L D S Y I R A A N A W P M L S A D E E R A L A E K L H Y H G D L E A A K T L I L S H L R F V V H I A R N Y A G Y G L P Q A D L I Q E G N I G L M K A V R R F N P E V G V R L V S F A V H W I K A E I H E Y V L R N W R I V K V ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Find nr database================================================ Query_seq: PI0721:PI0723|PI0721:PI0723:hypothetical protein:conserved hypothetical protein:->->:655043..656182 1140 tgatggatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtattcctacatcgcttatgccctgaagcagttccttgctcatgatgtggcgcacagaaatgcggagcgaatcgttgcgttttagtggaaaaatggcaatgcgttgttgtatttcgcttgttgtaatcccgcgctttccagccaatcttccatcgtcgtttatgatgttgcaagttacggtatcttggtagctgcgacttgttagtttctttcttttcttgtgtattacggtgcgttcgacgatgaacgtaatgttcttgtacgggtagctttgcgatgcccttacgcccaagtccatactgtacattccttcccattggcgtggaatgtagaagacagctgtgtcgttgcgctcccacccattgataggcaaagctttatagctgtcgtaggtgcgggcatctttgcacgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 672 Max: 1076 Len: 405 Subject: UniRef90_Q64NU5 Cluster: Hypothetical protein; n=2; Bacteroides fragilis|Rep: Hypothetical protein - Bacteroides fragilis HSP 1 e-value: 8.0E-6 bit: 54.3 Len: 405 Query Start:672 Query End:1076 Subject Strand: null Subject Start: 21 Subject End: 150 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... C K D A R T Y D S Y K A L P I N G W E R N D T A V F Y I P - R Q W E G M Y S M D L G V R A S Q S Y P Y K N I T F I V E R T V I H K K R K K L T S R S Y Q D T V T C N I I N D D G R L A G K R G I T T S E I Q Q R I A I F P L K R N D S L R I S V R H I M S K E L L Q G I S D V G ................................................................ ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... C D E N T V Y H S Y Q S T P P D G W K K S D T L F F N V P L K D S L A N L R L S V G V R N S S N Y P Y Q N L N I L I H Y N L E D S T V W K - - - - - - T D T L K F I L T D R E G K W T G T G W G S L Y Q S A L P L K D C F V K H P G N Y T F K I V H E M K N E Q L T G I S D V G ................................................................ tgatggatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 3 End: 209 .. M D N L A H L I Y R A H L V H W H T Y E P A L F R N G L H Y A L S N P P Y S I R N E L K S P C F V V F L S C F Y Q S Q I ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 7 End: 216 ...... M T L R T L F I E P I W F I G T R T S L L C S E M A C I M L C R I H H T A Y E M N L N P R V S S Y F L A A F I S P R F P ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 35 Strand: + Start: 259 End: 363 .................................................................................................................................................................................................................................................................. M A F V S L S R A F T S P C L I F C A N S T S S S K L T R S S R L M S ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 311 End: 400 ...................................................................................................................................................................................................................................................................................................................... M L T Q P L H L S S R G L R G L C L E G I F R V L C F L D L .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 363 End: 479 .......................................................................................................................................................................................................................................................................................................................................................................... M K V F F E C C A F S I C N R L G D F Q L S H L Y R G L I L R L D K C T Q K Q ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 53 Strand: + Start: 503 End: 661 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M I Y V F L R L F R R V L S V V L L F E K G N T V L L K R K Y Q L F R C P V S F C L S T F Y Y L F I C V L ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 580 End: 690 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M E K K V S T V Q M P C F V L F I Y L L L F V Y L R F M I R I P T S L M P .................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 52 Strand: + Start: 594 End: 749 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M N C S D A L F R F V Y L P F I I C L S A F Y D S Y S Y I A Y A L K Q F L A H D V A H R N A E R I V A F ....................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: + Start: 703 End: 855 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M L M M W R T E M R S E S L R F S G K M A M R C C I S L V V I P R F P A N L P S S F M M L Q V T V S W ............................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 771 End: 869 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M L Y F A C C N P A L S S Q S S I V V Y D V A S Y G I L V A A T C ............................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 888 End: 1139 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Y Y G A F D D E R N V L V R V A L R C P Y A Q V H T V H S F P L A W N V E D S C V V A L P P I D R Q S F I A V V G A G . Protein_Len: 51 Strand: - Start: 953 End: 1105 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ A W T W V T C E K G N A H F T S S L Q T T A S G G M S L C L K I A T T P A P M K A R L R Q L P L S Q M ................................... Protein_Len: 60 Strand: - Start: 651 End: 1115 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S Q S A R V G L D M S Y M G E W Q R P I Y F V A T D N R E W G N I P L A K Y S D Y T R A D K C A I A T I A V I A L A I M ......................... Protein_Len: 60 Strand: - Start: 643 End: 1026 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Y R P L Q S K N T E K K E Q T N R H T R R H V Y H E Q V P L K A I G K R G L G Y Q V N R G M P T S H L L C S H R Q A G M .................................................................................................................. Protein_Len: 59 Strand: - Start: 331 End: 507 .......................................................................................................................................................................................................................................................................................................................................... R L E R P R R P K H R S P I K R T S H K R S K Y D V P H N E T T E D T D H I S I L I P C I Y V F V T L Q A L F F L I M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 160 End: 252 ............................................................................................................................................................... S S K D T G S E R R Q Y P R S A I F R S K S I A K S G L R C M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 3 End: 437 .. N A K T L R E L A K V D G Q R I K Q A L E V E E D L S V L D E R S I D Q L Y K E L A T S E R N T I S Q T I K L Q R M Q M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tgatggatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatggatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0721:PI0723|PI0721:PI0723:hypothetical protein:conserved hypothetical protein:->->:655043..656182 1140 tgatggatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Intra-Species Hit: Count: 1 Min: 417 Max: 1140 Len: 724 Subject: pint_PI0721_PI0723|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[655043,656182]|1140 HSP 1 e-value: 1.0E-129 bit: 464.0 Len: 255 Query Start:417 Query End:671 Subject Strand: POSITIVE Subject Start: 417 Subject End: 671 ................................................................................................................................................................................................................................................................................................................................................................................................................................tttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgnnnnnnnacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtat..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ................................................................................................................................................................................................................................................................................................................................................................................................................................tttcagttgtctcatctgtatcgtggattgatattaagattggacaaatgtacacaaaaacagtgagttgagcaaggaaaaacagaataatatatgtttttttacgcctttttagaagagttttgtcagtcgttctgctatttgaaaagggaaatacggtcttattgaaaagaaagtatcaactgttcagatgccctgtttcgttttgtttatctaccttttattatttgtttatctgcgttttatgattcgtat..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 9.0E-28 bit: 127.0 Len: 64 Query Start:1077 Query End:1140 Subject Strand: POSITIVE Subject Start: 1077 Subject End: 1140 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgcaatcgctgtaatggcaacgattgcaagtgctatgatgtgcgaaaaggaatgtcctttccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUCAGUUGUCUCAUCUGUAUCGUGGAUUGAUAUUAAGAUUGGACAAAUGUACACAAAAACAGUGAGUUGAGCAAGGAAAAACAGAAUAAUAUAUGUUUUUUUACGCCUUUUUAGAAGAGUUUUGUCAGUCGUUCUGCUAUUUGAAAAGGGAAAUACGGUCUUAUUGAAAAGAAAGUAUCAACUGUUCAGAUGCCCUGUUUCGUUUUGUUUAUCUACCUUUUAUUAUUUGUUUAUCUGCGUUUUAUGAUUCGUAUUCCUACAUCGCUUAUGCCCUGAAGCAGUUCCUUGCUCAUGAUGUGGCGCACAGAAAUGCGGAGCGAAUCGUUGCGUUUUAGUGGAAAAAUGGCAAUGCGUUGUUGUAUUUCGCUUGUUGUAAUCCCGCGCUUUCCAGCCAAUCUUCCAUCGUCGUUUAUGAUGUUGCAAGUUACGGUAUCUUGGUAGCUGCGACUUGUUAGUUUCUUUCUUUUCUUGUGUAUUACGGUGCGUUCGACGAUGAACGUAAUGUUCUUGUACGGGUAGCUUUGCGAUGCCCUUACGCCCAAGUCCAUACUGUACAUUCCUUCCCAUUGGCGUGGAAUGUAGAAGACAGCUGUGUCGUUGCGCUCCCACCCAUUGAUAGGCAAAGCUUUAUAGCUGUCGUAGGUGCGGGCAUCUUUGCACGCAAUCGCUGUAAUGGCAACGAUUGCAAGUGCUAUGAUGUGCGAAAAGGAAUGUCCUUUCCUA ......((((((.((((((((((.....))))))..)))).))))))..((((((((((((..(((..(((((((((((...(((((..(((((..((((((((........))))))))...)))..))..))))).((((((((.((....((((..((((......))))..))))...)).))))))))..(((((((....(((.....(((...((((....(((......)))....))))...))).....)))..((....))...)))))))))))))))))).(((.((((...((....)).((((((....((((((....(((((((..(..((((....))))..).)))))))...))))))....)))..))).))))))).....)))..)))).....((((((.(((((.(.....).)))))))))))....................)))))))).(((.(((((((......))))))).)))..(((((((.(((((..((((((((((...((((...........(.((((((((..((....))...)))))))).).((((((((((...((((.((..((.....))..)))))).....))))))))))...)))).)))))))...))).(((((((.(((....))).)))))))....)))))..)))))))..(((((......))))). (-204.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGAAAGGACAUUCCUUUUCGCACAUCAUAGCACUUGCAAUCGUUGCCAUUACAGCGAUUGCGUGCAAAGAUGCCCGCACCUACGACAGCUAUAAAGCUUUGCCUAUCAAUGGGUGGGAGCGCAACGACACAGCUGUCUUCUACAUUCCACGCCAAUGGGAAGGAAUGUACAGUAUGGACUUGGGCGUAAGGGCAUCGCAAAGCUACCCGUACAAGAACAUUACGUUCAUCGUCGAACGCACCGUAAUACACAAGAAAAGAAAGAAACUAACAAGUCGCAGCUACCAAGAUACCGUAACUUGCAACAUCAUAAACGACGAUGGAAGAUUGGCUGGAAAGCGCGGGAUUACAACAAGCGAAAUACAACAACGCAUUGCCAUUUUUCCACUAAAACGCAACGAUUCGCUCCGCAUUUCUGUGCGCCACAUCAUGAGCAAGGAACUGCUUCAGGGCAUAAGCGAUGUAGGAAUACGAAUCAUAAAACGCAGAUAAACAAAUAAUAAAAGGUAGAUAAACAAAACGAAACAGGGCAUCUGAACAGUUGAUACUUUCUUUUCAAUAAGACCGUAUUUCCCUUUUCAAAUAGCAGAACGACUGACAAAACUCUUCUAAAAAGGCGUAAAAAAACAUAUAUUAUUCUGUUUUUCCUUGCUCAACUCACUGUUUUUGUGUACAUUUGUCCAAUCUUAAUAUCAAUCCACGAUACAGAUGAGACAACUGAAA ..(((((((.....)))))))((........((((..((((((((((......))))))))))))))...(((((((((.((((.(((((((.....((((..((((....))))..).)))..........)))))))...((((((((...((....))..))))))))...........)))).))..))))))).....))......((((((((((.....((((((((((.(((...)))..........................(((((..(...............)..)))))............)))))))))).(((((((((((((..(((((..........(((...........))).((((..((((......)))).))))........))))).))))))...)))).))).((((((((((.((((......((....))..((((....))))...........))))..((((((((((((.....((((..........((((.((((.............(((((..((((......))))..))))).)))))))).....((((.....))).)........)))).......((......))...))))))).))))).))))))))))......))))))))))..((.(((((..((((...((((.......)))).))))..))))).))... (-181.26) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 418 688 645 150 114 pint:PI1754|5end_conserved_hypothetical_protein_1681202..1681432_POSITIVE 398 687 643 212 167 pint:PI1743|5end_stationary-phase_survival_protein_1663948..1664178_POSITIVE 385 387 344 71 17 pint:PI1836|5end_conserved_hypothetical_protein_1777308..1777538_POSITIVE 384 650 601 72 26 pint:PI0806|5end_heavy_metal_efflux_pump_743679..743909_POSITIVE 377 650 601 70 3 pint:PI1599|5end_predicted_TPR-repeat-containing_protein_1527717..1527947_POSITIVE 373 610 570 207 179 pint:PI0635|5end_dephospho-CoA_kinase_580954..581184_POSITIVE 371 548 503 196 134 pint:PI1915|5end_conserved_hypothetical_protein_1858516..1858746_POSITIVE 371 347 303 117 63 pint:PI1459|5end_ribonucleotide_reductase,_alpha_subunit,_C-terminal_region_1381569..1381799_POSITIVE 370 689 641 79 34 pint:PI0093|5end_riboflavin_biosynthesis_protein_90489..90719_POSITIVE 363 316 274 193 148 pint:PI1081|5end_probable_endonuclease/resolvase_1000806..1001036_POSITIVE 361 319 271 140 78 pint:PI0834|5end_conserved_hypothetical_protein_770785..771015_POSITIVE 359 449 405 81 17 pint:PI0073|5end_dihydropteroate_synthase_70956..71186_POSITIVE 357 533 491 221 172 pint:PI1617|5end_hypothetical_protein_1542923..1543153_POSITIVE 357 650 601 134 78 pint:PI0785|5end_hypothetical_protein_723744..723974_POSITIVE 356 670 626 166 121 pint:PI1646|5end_hypothetical_protein_1561954..1562184_POSITIVE 355 669 621 161 113 pint:PI0118|5end_hypothetical_protein_111321..111551_POSITIVE 353 340 294 231 169 pint:PI0699|5end_conserved_hypothetical_protein_636647..636877_POSITIVE 353 320 271 225 168 pint:PI0362|5end_hypothetical_protein_333397..333627_POSITIVE 352 554 514 79 34 pint:PI1364|5end_dipeptidyl_peptidase_IV_1281862..1282092_POSITIVE 351 689 643 218 180 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 418 688 645 138 102 pint:PI1752|3end_conserved_hypothetical_protein_1681270..1681420_POSITIVE 418 688 645 150 114 pint:PI1753|3end_conserved_hypothetical_protein_1681282..1681432_POSITIVE 384 650 601 120 74 pint:PI0805|3end_conserved_hypothetical_protein_743807..743957_POSITIVE 371 347 303 94 40 pint:PI1458|3end_dihydroneopterin_aldolase_1381626..1381776_POSITIVE 364 687 647 40 5 pint:PI1741|3end_lipid-A-disaccharide_synthase_1663856..1664006_POSITIVE 358 682 645 39 1 pint:PI0040|3end_conserved_hypothetical_protein_(possible_integral_membrane_protein)_41872..42022_POSITIVE 355 669 621 74 26 pint:PI0117|3end_ribonuclease_HI_111314..111464_POSITIVE 352 554 514 59 14 pint:PI1363|3end_hypothetical_protein_1281922..1282072_POSITIVE 347 330 281 84 17 pint:PI1943|3end_hypothetical_protein_1902206..1902356_POSITIVE 347 607 564 108 54 pint:PI1227|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148974..1149124_POSITIVE 347 446 406 85 37 pint:PI0728|3end_integrase_661508..661658_POSITIVE 346 694 651 146 93 pint:PI0435|3end_30S_ribosomal_protein_S21_407265..407415_POSITIVE 345 678 631 52 5 pint:PI0249|3end_conserved_hypothetical_protein_247026..247176_POSITIVE 342 373 335 79 38 pint:PI1258|3end_elongation_factor_Tu_1180626..1180776_POSITIVE 339 710 662 151 106 pint:PI0667|3end_hypothetical_protein_610477..610627_POSITIVE 338 330 281 56 12 pint:PI1670|3end_hypothetical_protein_1587026..1587176_POSITIVE 335 678 634 135 84 pint:PI1716|3end_hypothetical_protein_1632006..1632156_POSITIVE 330 390 346 150 107 pint:PI2090|3end_thioesterase_family_protein_2048066..2048216_POSITIVE 330 555 515 110 66 pint:PI1781|3end_membrane-associated_zinc_metalloprotease_1714551..1714701_POSITIVE 330 609 563 109 48 pint:PI1398|3end_probable_imidazolonepropionase_1320672..1320822_POSITIVE