Origin IGS: taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttttgttgaatctttattattttaatttattgatacaatctaaattcttttctgttatctatcagtactttttacaccgatattttaatcgattacaaagatacactttctatttcaatattgtttgtaataagttcaatatttattttttattcacaatttaaggcagaacagagaataaaataaggtataacaagccgttaaggtgtacttgaagagtgagcgaaagccatcagaatttctcttgaatacagcttcgatgtaagtctgcctatacttaacgcacaaattctgcctatcgccctgcatctttccgtcttaaaaactgtataaaagtgtttagtgtaaaaactcgatttgaacaagcaaaatagagttgtgaaacacccaaataagcagttaatcgtccaagaaaaaagttttcagattctaaaagagccgctttcgcatagcgaaagcggctcttttacacggtaaaagctactcttttgcaacgcaaaacatacgcttttacaagctaattctattctttatatcatatttattggttgttttgcgagcacaatctttccatttcattgtacaaacatcacacacacaaacataaacagctaataattaagccattaaacttgcacacaaatgctgcaattatttgcaaataattcttctttcacgcaaaaaagccaccttctcgccgttgttccacgcttgaattttaacaagtagctacttttatttcaccgaaattaacaaacgagtgaaaagcattataaaccaccttactacctatttgtaacaaaaaagctgcgcaccaatgttttataggtgcgcagctaatatacttattgtatagacatctatgtgcaatctgcacaatatgcccactcttta Mask Tandem Repeat Region ================================================ taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Find is-nt database================================================ Query_seq: PI0713:PI0714|PI0713:PI0714:hypothetical protein:conserved hypothetical protein:->->:650590..651484 895 taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0713:PI0714|PI0713:PI0714:hypothetical protein:conserved hypothetical protein:->->:650590..651484 895 taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0713:PI0714|PI0713:PI0714:hypothetical protein:conserved hypothetical protein:->->:650590..651484 895 taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 53 Strand: + Start: 15 End: 173 .............. M V Q I A H R C L Y N K Y I S C A P I K H W C A A F L L Q I G S K V V Y N A F H S F V N F G E I K V A T C .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: + Start: 170 End: 265 ......................................................................................................................................................................... M L K F K R G T T A R R W L F C V K E E L F A N N C S I C V Q V ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 368 End: 469 ............................................................................................................................................................................................................................................................................................................................................................................... M E L A C K S V C F A L Q K S S F Y R V K E P L S L C E S G S F R I .......................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 504 End: 596 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M G V S Q L Y F A C S N R V F T L N T F I Q F L R R K D A G R ........................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 491 End: 691 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M N C L F G C F T T L F C L F K S S F Y T K H F Y T V F K T E R C R A I G R I C A L S I G R L T S K L Y S R E I L M A F ............................................................................................................................................................................................................ Protein_Len: 51 Strand: + Start: 586 End: 738 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Q G D R Q N L C V K Y R Q T Y I E A V F K R N S D G F R S L F K Y T L T A C Y T L F Y S L F C L K L ............................................................................................................................................................. Protein_Len: 53 Strand: - Start: 734 End: 892 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. I T F L F Y I N F K N C V I N F Y F T Y R Q L R N F Y R H L F Y Q Y I V S F F K S Q I L L N F Y Y L N L M ... Protein_Len: 44 Strand: - Start: 712 End: 843 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... K I R Q E A K F Q S Y F I F I S S I V F L I S I S L T D K Y D I L I D T Y F T S I S L M .................................................... Protein_Len: 33 Strand: - Start: 565 End: 663 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... V T K L V S L H L A I P L I Q A N L I P L S V D F S Y E L S I R M ........................................................................................................................................................................................................................................ Protein_Len: 39 Strand: - Start: 421 End: 537 .................................................................................................................................................................................................................................................................................................................................................................................................................................... R T F S G S E S H S L P E K L I Q F S K E Q V I L Q K N P H K V V R N Q K N M ...................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 291 End: 446 .................................................................................................................................................................................................................................................................................................. T Q T H S T Q V I F H F S Q A R L V V L L Y S I F F L I L K Y F R I N Q T A F S Y S K G H L L A A K A M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 41 Strand: - Start: 233 End: 355 ........................................................................................................................................................................................................................................ K C I I A A N T H L N L P K I I L Q K H K H T H H K Y L S I S L N H E C F L W Y M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 34 Strand: - Start: 2 End: 103 . F L P C I T C I A C L H R Y L L Y I L Q A G I F C Q H A A K K N C M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 150 End: 179 .....................................................................................................................................................GGTGAAATAAAAGTAGCTACTTGTTAAAAT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 356 End: 385 ...................................................................................................................................................................................................................................................................................................................................................................TTTACAAGCTAATTCTATTCTTTATATCAT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 77 HP_score: -10.1 Tail_Score: -3.05514 Start: 55 End: 91 Full_Region: cctatttgtaacaaa aaagctgcgcaccaatg ttt tataggtgcgcagctaa tatacttattgtata ......................................................aaagctgcgcaccaatgttttataggtgcgcagctaa.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -15.5 Tail_Score: -5.45652 Start: 58 End: 88 Full_Region: ACAATAAGTATATTA GCTGCGCACCTAT AAAAC ATTGGTGCGCAGC TTTTTTGTTACAAAT .........................................................GCTGCGCACCTATAAAACATTGGTGCGCAGC....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -16.2 Tail_Score: -2.73622 Start: 58 End: 88 Full_Region: atttgtaacaaaaaa gctgcgcaccaatg ttt tataggtgcgcagc taatatacttattgt .........................................................gctgcgcaccaatgttttataggtgcgcagc....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -23.3 Tail_Score: -4.16943 Start: 431 End: 459 Full_Region: TTTTACCGTGTAAAA GAGCCGCTTTCGC TAT GCGAAAGCGGCTC TTTTAGAATCTGAAA ..............................................................................................................................................................................................................................................................................................................................................................................................................................................GAGCCGCTTTCGCTATGCGAAAGCGGCTC.................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -23.3 Tail_Score: -4.09685 Start: 431 End: 459 Full_Region: tttcagattctaaaa gagccgctttcgc ata gcgaaagcggctc ttttacacggtaaaa ..............................................................................................................................................................................................................................................................................................................................................................................................................................................gagccgctttcgcatagcgaaagcggctc.................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PI0713:PI0714|PI0713:PI0714:hypothetical protein:conserved hypothetical protein:->->:650590..651484 895 taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 895 Len: 895 Subject: pint_PI0713_PI0714|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[650590,651484]|895 HSP 1 e-value: 0.0 bit: 1774.0 Len: 895 Query Start:1 Query End:895 Subject Strand: POSITIVE Subject Start: 1 Subject End: 895 taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa taaagagtgggcatattgtgcagattgcacatagatgtctatacaataagtatattagctgcgcacctataaaacattggtgcgcagcttttttgttacaaataggtagtaaggtggtttataatgcttttcactcgtttgttaatttcggtgaaataaaagtagctacttgttaaaattcaagcgtggaacaacggcgagaaggtggcttttttgcgtgaaagaagaattatttgcaaataattgcagcatttgtgtgcaagtttaatggcttaattattagctgtttatgtttgtgtgtgtgatgtttgtacaatgaaatggaaagattgtgctcgcaaaacaaccaataaatatgatataaagaatagaattagcttgtaaaagcgtatgttttgcgttgcaaaagagtagcttttaccgtgtaaaagagccgctttcgctatgcgaaagcggctcttttagaatctgaaaacttttttcttggacgattaactgcttatttgggtgtttcacaactctattttgcttgttcaaatcgagtttttacactaaacacttttatacagtttttaagacggaaagatgcagggcgataggcagaatttgtgcgttaagtataggcagacttacatcgaagctgtattcaagagaaattctgatggctttcgctcactcttcaagtacaccttaacggcttgttataccttattttattctctgttctgccttaaattgtgaataaaaaataaatattgaacttattacaaacaatattgaaatagaaagtgtatctttgtaatcgattaaaatatcggtgtaaaaagtactgatagataacagaaaagaatttagattgtatcaataaattaaaataataaagattcaacaaaaa HSP 2 e-value: 9.0E-6 bit: 54.0 Len: 39 Query Start:426 Query End:464 Subject Strand: NEGATIVE Subject Start: 426 Subject End: 464 .........................................................................................................................................................................................................................................................................................................................................................................................................................................taaaagagccgctttcgctatgcgaaagcggctctttta............................................................................................................................................................................................................................................................................................................................................................................................................................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................................taaaagagccgctttcgcatagcgaaagcggctctttta............................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGAGUGGGCAUAUUGUGCAGAUUGCACAUAGAUGUCUAUACAAUAAGUAUAUUAGCUGCGCACCUAUAAAACAUUGGUGCGCAGCUUUUUUGUUACAAAUAGGUAGUAAGGUGGUUUAUAAUGCUUUUCACUCGUUUGUUAAUUUCGGUGAAAUAAAAGUAGCUACUUGUUAAAAUUCAAGCGUGGAACAACGGCGAGAAGGUGGCUUUUUUGCGUGAAAGAAGAAUUAUUUGCAAAUAAUUGCAGCAUUUGUGUGCAAGUUUAAUGGCUUAAUUAUUAGCUGUUUAUGUUUGUGUGUGUGAUGUUUGUACAAUGAAAUGGAAAGAUUGUGCUCGCAAAACAACCAAUAAAUAUGAUAUAAAGAAUAGAAUUAGCUUGUAAAAGCGUAUGUUUUGCGUUGCAAAAGAGUAGCUUUUACCGUGUAAAAGAGCCGCUUUCGCUAUGCGAAAGCGGCUCUUUUAGAAUCUGAAAACUUUUUUCUUGGACGAUUAACUGCUUAUUUGGGUGUUUCACAACUCUAUUUUGCUUGUUCAAAUCGAGUUUUUACACUAAACACUUUUAUACAGUUUUUAAGACGGAAAGAUGCAGGGCGAUAGGCAGAAUUUGUGCGUUAAGUAUAGGCAGACUUACAUCGAAGCUGUAUUCAAGAGAAAUUCUGAUGGCUUUCGCUCACUCUUCAAGUACACCUUAACGGCUUGUUAUACCUUAUUUUAUUCUCUGUUCUGCCUUAAAUUGUGAAUAAAAAAUAAAUAUUGAACUUAUUACAAACAAUAUUGAAAUAGAAAGUGUAUCUUUGUAAUCGAUUAAAAUAUCGGUGUAAAAAGUACUGAUAGAUAACAGAAAAGAAUUUAGAUUGUAUCAAUAAAUUAAAAUAAUAAAGAUUCAACAAAAA ......((((((((..(((((.....)))))...))))))))..(((((((.....(((((((((((...........)))))))))))((((((((((((..((((((..((((((((.........((((((.((...........)))))))).......))))))))..((((((..(((.(((.(((((.(.((..((((((((((...((.(((((((.((((((.(((.........((((((.((((.(((.(((((..((((((........))))))((((((((((...(((..(((..(((((((...........)))))))..)))..)))....)))))))))).................(((((((..(((((((((((((((((((..(((((.((.(((((...(.((((((((((((((((((...)))))))))))))))))).)...))))).))...)))))..........((((.......(((((.....(((((...((((......))))..)))))...))))).....(((((.....(((.....))).)))))..)))).)))))..)))))))..)))))))...))))))).))))).))))))))))))).....))).)))))).....))))))).))......)))).))))))..)).)))))).)))))))))))).........))))))....))))).)))))))...........)))))))......................((..((((((((..((..(((...((((((((.......))))))))..)))..))......(((((.((((....)))).)))))...))))))))...))..... (-237.93) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUGUUGAAUCUUUAUUAUUUUAAUUUAUUGAUACAAUCUAAAUUCUUUUCUGUUAUCUAUCAGUACUUUUUACACCGAUAUUUUAAUCGAUUACAAAGAUACACUUUCUAUUUCAAUAUUGUUUGUAAUAAGUUCAAUAUUUAUUUUUUAUUCACAAUUUAAGGCAGAACAGAGAAUAAAAUAAGGUAUAACAAGCCGUUAAGGUGUACUUGAAGAGUGAGCGAAAGCCAUCAGAAUUUCUCUUGAAUACAGCUUCGAUGUAAGUCUGCCUAUACUUAACGCACAAAUUCUGCCUAUCGCCCUGCAUCUUUCCGUCUUAAAAACUGUAUAAAAGUGUUUAGUGUAAAAACUCGAUUUGAACAAGCAAAAUAGAGUUGUGAAACACCCAAAUAAGCAGUUAAUCGUCCAAGAAAAAAGUUUUCAGAUUCUAAAAGAGCCGCUUUCGCAUAGCGAAAGCGGCUCUUUUACACGGUAAAAGCUACUCUUUUGCAACGCAAAACAUACGCUUUUACAAGCUAAUUCUAUUCUUUAUAUCAUAUUUAUUGGUUGUUUUGCGAGCACAAUCUUUCCAUUUCAUUGUACAAACAUCACACACACAAACAUAAACAGCUAAUAAUUAAGCCAUUAAACUUGCACACAAAUGCUGCAAUUAUUUGCAAAUAAUUCUUCUUUCACGCAAAAAAGCCACCUUCUCGCCGUUGUUCCACGCUUGAAUUUUAACAAGUAGCUACUUUUAUUUCACCGAAAUUAACAAACGAGUGAAAAGCAUUAUAAACCACCUUACUACCUAUUUGUAACAAAAAAGCUGCGCACCAAUGUUUUAUAGGUGCGCAGCUAAUAUACUUAUUGUAUAGACAUCUAUGUGCAAUCUGCACAAUAUGCCCACUCUUUA ....((((((((..(((((((...(((.((((((............(((((.(((........)))............((((......)))).....))))).............))))))..))).)))))))))))))))((((((((((.(((.............))).))))))))))....((((((..(((.((..((((((((..(((...(((.((....)))))((((((((...(((((......)))))..(((((........)))))......))))))))....)))...))))))))..)).))).....((((((((((((((.((((((....(((((....((((((((.....((((((((........((((((((((((.....((.((((...((((.....(((...((((((((((((((((((...))))))))))))))))))....((((((((.....((((((...)))))).....))))))))........)))...))))......((.(((((((((((((((.((......))............(((....))).............)).))))))))))))).))..........)))).))......)))))..)))))))...))))))))........((......)).........))..))))))...(((((........)))))..............................))))).....))))))................................(((((((((((...........)))))))))))...)))))).)))))))).......(((((.....))))).))))))......... (-192.03) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 690 440 392 59 11 pint:PI1528|5end_hypothetical_protein_1449685..1449915_POSITIVE 639 440 392 76 28 pint:PI1934|5end_hypothetical_protein_1882363..1882593_POSITIVE 613 440 392 125 77 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 587 430 381 74 25 pint:PI2128|5end_hypothetical_protein_2076512..2076742_POSITIVE 581 440 392 218 170 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 577 440 394 64 18 pint:PI2144|5end_hypothetical_protein_2091954..2092184_POSITIVE 574 440 394 76 30 pint:PI1694|5end_hypothetical_protein_1609717..1609947_POSITIVE 573 440 394 103 57 pint:PI1604|5end_hypothetical_protein_1534017..1534247_POSITIVE 565 440 392 76 28 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 554 440 393 48 1 pint:PI2131|5end_hypothetical_protein_2080678..2080908_POSITIVE 552 456 414 53 8 pint:PI1356|5end_hypothetical_protein_1273162..1273392_POSITIVE 548 440 393 48 1 pint:PI0004|5end_hypothetical_protein_6199..6429_POSITIVE 546 430 384 203 157 pint:PI0159|5end_hypothetical_protein_151273..151503_POSITIVE 546 430 384 115 69 pint:PI0157|5end_hypothetical_protein_151185..151415_POSITIVE 545 464 422 210 167 pint:PI1870|5end_hypothetical_protein_1815302..1815532_POSITIVE 542 440 393 48 1 pint:PI1733|5end_hypothetical_protein_1652160..1652390_POSITIVE 542 440 393 48 1 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 539 423 381 82 43 pint:PI0322|5end_hypothetical_protein_303508..303738_POSITIVE 536 440 392 84 36 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 536 440 392 76 28 pint:PI1167|5end_hypothetical_protein_1089688..1089918_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 677 430 381 148 99 pint:PI0551|3end_hypothetical_protein_502683..502833_POSITIVE 613 440 392 118 70 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 613 440 392 59 11 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 596 430 384 148 102 pint:PI2131|3end_hypothetical_protein_2080868..2081018_POSITIVE 569 430 384 142 96 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 552 456 414 151 106 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 546 430 384 148 102 pint:PI0158|3end_hypothetical_protein_151298..151448_POSITIVE 522 440 394 59 13 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 515 452 414 39 1 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 493 440 394 118 72 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 490 430 384 148 102 pint:PI0200|3end_hypothetical_protein_191735..191885_POSITIVE 477 430 384 148 102 pint:PI1340|3end_hypothetical_protein_1256872..1257022_POSITIVE 468 429 384 151 106 pint:PI1922|3end_hypothetical_protein_1865454..1865604_POSITIVE 467 450 401 124 69 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 456 440 398 150 108 pint:PI0755|3end_hypothetical_protein_686884..687034_POSITIVE 445 430 384 148 102 pint:PI2011|3end_hypothetical_protein_1970730..1970880_POSITIVE 441 452 414 43 5 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 441 452 414 43 5 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 432 460 411 134 86 pint:PI1761|3end_hypothetical_protein_1684543..1684693_POSITIVE 427 455 411 101 57 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE