Origin IGS: tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcttgttctctttatataatggtgcaaagataacgaaaaaataaggaataaccacgtttttctgtcgaataatagacgaattaccgacaagaaaacgtggttattccttagcgaaagcaatgttttcta Mask Tandem Repeat Region ================================================ tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Find is-nt database================================================ Query_seq: PI0703:PI0704|PI0703:PI0704:hypothetical protein:conserved hypothetical protein:->->:640257..640386 130 tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0703:PI0704|PI0703:PI0704:hypothetical protein:conserved hypothetical protein:->->:640257..640386 130 tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0703:PI0704|PI0703:PI0704:hypothetical protein:conserved hypothetical protein:->->:640257..640386 130 tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 10 End: 129 ......... M L S L R N N H V F L S V I R L L F D R K T W L F L I F S L S L H H Y I K R T R . tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 95 HP_score: -12.6 Tail_Score: -3.79788 Start: 27 End: 81 Full_Region: GCTTTCGCTAAGGAA TAACCACGTTTTCTTGTCGGTAAT TCGTCT ATTATTCGACAGAAAAACGTGGTTA TTCCTTATTTTTTCG ..........................TAACCACGTTTTCTTGTCGGTAATTCGTCTATTATTCGACAGAAAAACGTGGTTA................................................. TransTerm Strand: + Conf: 87 HP_score: -9.9 Tail_Score: -3.97003 Start: 30 End: 78 Full_Region: TTCGCTAAGGAATAA CCACGTTTTCTTGTCGGTAAT TCGTCT ATTATTCGACAGAAAAACGTGG TTATTCCTTATTTTT .............................CCACGTTTTCTTGTCGGTAATTCGTCTATTATTCGACAGAAAAACGTGG.................................................... TransTerm Strand: - Conf: 73 HP_score: -7.7 Tail_Score: -3.81146 Start: 30 End: 78 Full_Region: aaaaataaggaataa ccacgtttttctgtcgaataat agacga attaccgacaagaaaacgtgg ttattccttagcgaa .............................ccacgtttttctgtcgaataatagacgaattaccgacaagaaaacgtgg.................................................... Find igs database================================================ Query_seq: PI0703:PI0704|PI0703:PI0704:hypothetical protein:conserved hypothetical protein:->->:640257..640386 130 tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Intra-Species Hit: Count: 1 Min: 1 Max: 130 Len: 130 Subject: pint_PI0703_PI0704|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[640257,640386]|130 HSP 1 e-value: 4.0E-68 bit: 258.0 Len: 130 Query Start:1 Query End:130 Subject Strand: POSITIVE Subject Start: 1 Subject End: 130 tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat tagaaaacattgctttcgctaaggaataaccacgttttcttgtcggtaattcgtctattattcgacagaaaaacgtggttattccttattttttcgttatctttgcaccattatataaagagaacaagat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAAACAUUGCUUUCGCUAAGGAAUAACCACGUUUUCUUGUCGGUAAUUCGUCUAUUAUUCGACAGAAAAACGUGGUUAUUCCUUAUUUUUUCGUUAUCUUUGCACCAUUAUAUAAAGAGAACAAGAU ..((((.......))))..(((((((((((((((((((..((((((((((......)))).))))))..))))))))))))))))))).......(((.((((((..........)))))).)))..... (-42.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCUUGUUCUCUUUAUAUAAUGGUGCAAAGAUAACGAAAAAAUAAGGAAUAACCACGUUUUUCUGUCGAAUAAUAGACGAAUUACCGACAAGAAAACGUGGUUAUUCCUUAGCGAAAGCAAUGUUUUCUA ...(((((.(((((.(((....))).))))).))))).....(((((((((((((((((((..(((((..((((......)))).)))))..)))))))))))))))))))((....))........... (-46.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 55 11 85 38 pint:PI0341|5end_conserved_hypothetical_protein_317038..317268_POSITIVE 278 109 67 175 134 pint:PI0524|5end_meso-diaminopimelate_D-dehydrogenase_479873..480103_POSITIVE 274 70 23 58 8 pint:PI1337|5end_conserved_hypothetical_protein_1254406..1254636_POSITIVE 274 73 33 143 99 pint:PI0022|5end_hypothetical_protein_25641..25871_POSITIVE 263 64 23 86 49 pint:PI0601|5end_hypothetical_protein_548758..548988_POSITIVE 256 58 13 52 6 pint:PI0653|5end_hypothetical_protein_596046..596276_POSITIVE 256 48 8 46 1 pint:PI0040|5end_conserved_hypothetical_protein_(possible_integral_membrane_protein)_41010..41240_POSITIVE 253 112 71 122 86 pint:PI0839|5end_conserved_hypothetical_protein_773843..774073_POSITIVE 249 55 17 231 185 pint:PI1572|5end_hypothetical_protein_1497782..1498012_POSITIVE 249 78 31 230 192 pint:PI0751|5end_conserved_hypothetical_protein_680623..680853_POSITIVE 249 56 11 151 98 pint:PI0546|5end_hypothetical_protein_497799..498029_POSITIVE 246 54 13 206 159 pint:PI1744|5end_chromosome_partitioning_protein_1665635..1665865_POSITIVE 245 68 32 226 202 pint:PI0542|5end_hypothetical_protein_495554..495784_POSITIVE 243 57 11 214 178 pint:PI1413|5end_hypothetical_protein_1333727..1333957_POSITIVE 243 109 63 64 15 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 241 52 11 53 9 pint:PI1628|5end_hypothetical_protein_1551082..1551312_POSITIVE 240 107 61 184 124 pint:PI0728|5end_integrase_660427..660657_POSITIVE 240 56 13 213 175 pint:PI0617|5end_electron_transfer_flavoprotein,_alpha_subunit_566367..566597_POSITIVE 239 57 11 158 118 pint:PI2109|5end_surface_antigen_BspA_2060553..2060783_POSITIVE 239 50 8 61 18 pint:PI1627|5end_hypothetical_protein_1550445..1550675_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 70 21 54 4 pint:PI0703|3end_hypothetical_protein_640196..640346_POSITIVE 278 109 67 66 25 pint:PI0523|3end_hypothetical_protein_479844..479994_POSITIVE 274 73 33 70 26 pint:PI0021|3end_orotate_phosphoribosyltransferase_25648..25798_POSITIVE 263 64 23 45 8 pint:PI0600|3end_bifunctional_purine_biosynthesis_protein,_5-aminoimidazole-4-carboxamide_ribonucleotide(AICAR)_transformylase/IMP_cyclohydrolase_548797..548947_POSITIVE 254 115 72 118 74 pint:PI1494|3end_conserved_hypothetical_protein_1413968..1414118_POSITIVE 254 77 31 93 43 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 253 112 71 126 90 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE 253 54 17 29 2 pint:PI0230|3end_hypothetical_protein_228906..229056_POSITIVE 249 56 11 139 86 pint:PI0545|3end_sugar_kinase_497867..498017_POSITIVE 246 47 5 144 104 pint:PI1708|3end_hypothetical_protein_1625562..1625712_POSITIVE 245 79 31 66 24 pint:PI0951|3end_hypothetical_protein_885448..885598_POSITIVE 244 57 11 135 97 pint:PI0675|3end_hypothetical_protein_613957..614107_POSITIVE 241 52 11 48 4 pint:PI1627|3end_hypothetical_protein_1551157..1551307_POSITIVE 239 57 11 150 110 pint:PI2108|3end_cell_surface_protein;_possible_cell_surface_antigen_BspA_2060625..2060775_POSITIVE 239 50 8 58 15 pint:PI1626|3end_hypothetical_protein_1550522..1550672_POSITIVE 239 44 8 53 22 pint:PI1008|3end_cytochrome_c_nitrite_reductase,_small_subunit_929331..929481_POSITIVE 237 76 40 115 85 pint:PI0085|3end_RNase_P_protein_component_84960..85110_POSITIVE 237 86 45 61 16 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE 236 66 35 127 92 pint:PI1101|3end_hypothetical_protein_1025315..1025465_POSITIVE 236 55 17 41 3 pint:PI0039|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_41077..41227_POSITIVE