Origin IGS: aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagcgtctgtccacccagcacgactgcgaaaaacgtgtctaccgaatcggtttaggcattcattttcgcaatcagacgaattatttacacgtaaagaaatatttatttacgtgtagaaaaatatttacttacacgtagagaaattattatttacacgtaaataatttaagcgacaagaattt Mask Tandem Repeat Region ================================================ aaattcttgtcgcttaaattattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Find is-nt database================================================ Query_seq: PI0701:PI0702|PI0701:PI0702:hypothetical protein:conserved hypothetical protein; possible tetracenomycin C synthesis protein homolog:->->:638598..638779 182 aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0701:PI0702|PI0701:PI0702:hypothetical protein:conserved hypothetical protein; possible tetracenomycin C synthesis protein homolog:->->:638598..638779 182 aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0701:PI0702|PI0701:PI0702:hypothetical protein:conserved hypothetical protein; possible tetracenomycin C synthesis protein homolog:->->:638598..638779 182 aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 77 End: 181 ............................................................................ M N I S L R V N N S S D C E N E C L N R F G R H V F R S R A G W T D A . Protein_Len: 30 Strand: - Start: 70 End: 159 ..................................................................... V Y I F I E K R T F L E D S Q S F S H R F R N P L C T K R M ....................... Protein_Len: 43 Strand: - Start: 48 End: 176 ............................................... T Y T F I K R C T F L Y K K V H L Y N T Q N R F H I G L G I R Y V R K E C D H Q T S M ...... aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0701:PI0702|PI0701:PI0702:hypothetical protein:conserved hypothetical protein; possible tetracenomycin C synthesis protein homolog:->->:638598..638779 182 aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Intra-Species Hit: Count: 1 Min: 1 Max: 182 Len: 182 Subject: pint_PI0701_PI0702|hypothetical protein:conserved hypothetical protein; possible tetracenomycin C synthesis protein homolog|POSITIVE:POSITIVE|[638598,638779]|182 HSP 1 e-value: 5.0E-99 bit: 361.0 Len: 182 Query Start:1 Query End:182 Subject Strand: POSITIVE Subject Start: 1 Subject End: 182 aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta aaattcttgtcgcttaaattatttacgtgtaaataataatttctctacgtgtaagtaaatatttttctacacgtaaataaatatttctttacgtgtaaataattcgtctgattgcgaaaatgaatgcctaaaccgattcggtagacacgtttttcgcagtcgtgctgggtggacagacgcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUUCUUGUCGCUUAAAUUAUUUACGUGUAAAUAAUAAUUUCUCUACGUGUAAGUAAAUAUUUUUCUACACGUAAAUAAAUAUUUCUUUACGUGUAAAUAAUUCGUCUGAUUGCGAAAAUGAAUGCCUAAACCGAUUCGGUAGACACGUUUUUCGCAGUCGUGCUGGGUGGACAGACGCUA ......(((((((((.(((((((((((((((((.((..((((.(.((((((((.(.(((....)))))))))))).).))))..)).))))))))))))))))).((.((((((((((((....((.((..((((...)))))).))...)))))))))))).)).)))).)))))...... (-46.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCGUCUGUCCACCCAGCACGACUGCGAAAAACGUGUCUACCGAAUCGGUUUAGGCAUUCAUUUUCGCAAUCAGACGAAUUAUUUACACGUAAAGAAAUAUUUAUUUACGUGUAGAAAAAUAUUUACUUACACGUAGAGAAAUUAUUAUUUACACGUAAAUAAUUUAAGCGACAAGAAUUU .....(((((((.........((.((((((((..((((((((((...))))..))))))...)))))))).))....(((((((((((..(((((.....((((.(((((((((((............))))))))))).)))).....)))))..)))))))))))..).))).))).... (-49.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 532 70 21 106 57 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 518 100 51 185 136 pint:PI0115|5end_hypothetical_protein_108062..108292_POSITIVE 518 100 51 171 122 pint:PI0111|5end_hypothetical_protein_108048..108278_POSITIVE 518 100 51 136 87 pint:PI0112|5end_hypothetical_protein_108013..108243_POSITIVE 484 89 41 142 93 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 474 60 12 226 179 pint:PI0311|5end_hypothetical_protein_294721..294951_POSITIVE 474 106 63 168 124 pint:PI0130|5end_hypothetical_protein_122032..122262_POSITIVE 447 58 11 215 166 pint:PI0173|5end_hypothetical_protein_165305..165535_POSITIVE 415 99 51 117 69 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 412 180 132 224 177 pint:PI1228|5end_conserved_hypothetical_protein_1149045..1149275_POSITIVE 400 78 46 231 199 pint:PI0599|5end_hypothetical_protein_547376..547606_POSITIVE 378 76 46 138 108 pint:PI1462|5end_hypothetical_protein_1385094..1385324_POSITIVE 373 167 137 99 70 pint:PI0498|5end_integrase_459351..459581_POSITIVE 365 176 136 77 36 pint:PI1150|5end_30S_ribosomal_protein_S6_1075683..1075913_POSITIVE 365 177 132 100 51 pint:PI0094|5end_protoporphyrinogen_oxidase_89571..89801_POSITIVE 364 100 53 155 109 pint:PI0983|5end_hypothetical_protein_909750..909980_POSITIVE 357 89 41 130 81 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 343 167 128 229 180 pint:PI0629|5end_heat_shock_protein_15_576629..576859_POSITIVE 332 168 125 81 32 pint:PI0363|5end_hypothetical_protein_332832..333062_POSITIVE 331 178 140 61 4 pint:PI0699|5end_conserved_hypothetical_protein_636647..636877_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 507 110 63 62 14 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 450 70 21 113 64 pint:PI2105|3end_hypothetical_protein_2059586..2059736_POSITIVE 447 58 11 68 19 pint:PI0172|3end_ISPg3-related_transposase_165238..165388_POSITIVE 412 180 132 73 26 pint:PI1227|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148974..1149124_POSITIVE 391 110 69 52 11 pint:PI0808|3end_ISPg3-related_transposase_746457..746607_POSITIVE 378 76 46 37 7 pint:PI1461|3end_conserved_hypothetical_protein_1385073..1385223_POSITIVE 373 167 137 43 14 pint:PI0497|3end_predicted_recombinase,_tnpA_protein_459375..459525_POSITIVE 368 171 133 71 18 pint:PI0042|3end_hypothetical_protein_43444..43594_POSITIVE 365 176 136 71 30 pint:PI1149|3end_30S_ribosomal_protein_S18_1075757..1075907_POSITIVE 365 177 132 100 51 pint:PI0092|3end_regulatory_protein_RecX_89651..89801_POSITIVE 353 123 88 42 4 pint:PI0491|3end_hypothetical_protein_451227..451377_POSITIVE 309 156 112 69 24 pint:PI1498|3end_probable_membrane_associated_lipoprotein_1419988..1420138_POSITIVE 307 178 133 60 6 pint:PI1395|3end_hypothetical_protein_1314407..1314557_POSITIVE 304 180 133 113 58 pint:PI0369|3end_hypothetical_protein_340409..340559_POSITIVE 301 177 135 102 57 pint:PI0955|3end_conserved_hypothetical_protein_886084..886234_POSITIVE 296 179 131 105 70 pint:PI1716|3end_hypothetical_protein_1632006..1632156_POSITIVE 296 170 127 109 58 pint:PI0583|3end_hypothetical_protein_526276..526426_POSITIVE 294 179 131 151 82 pint:PI1736|3end_topoisomerase_IV,_subunit_A_1657090..1657240_POSITIVE 294 174 133 137 84 pint:PI1606|3end_conserved_hypothetical_protein_1535565..1535715_POSITIVE 293 180 133 73 5 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE