Origin IGS: cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggctttaacatcgttatcagcacttttccgcagcacgttttaacaacaaatatatatgaaaagaattttatttgcatttatggcattagcattaagcagttttggagcg Mask Tandem Repeat Region ================================================ cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Find is-nt database================================================ Query_seq: PI0697:PI0698|PI0697:PI0698:5'-nucleotidase family protein:conserved hypothetical protein:->->:635401..635511 111 cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0697:PI0698|PI0697:PI0698:5'-nucleotidase family protein:conserved hypothetical protein:->->:635401..635511 111 cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0697:PI0698|PI0697:PI0698:5'-nucleotidase family protein:conserved hypothetical protein:->->:635401..635511 111 cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 2 End: 100 . S W F Q K I S I G Y I C I F N K E Y I N T T L V H Q P F T S I V M ........... cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0697:PI0698|PI0697:PI0698:5'-nucleotidase family protein:conserved hypothetical protein:->->:635401..635511 111 cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Intra-Species Hit: Count: 1 Min: 1 Max: 111 Len: 111 Subject: pint_PI0697_PI0698|5'-nucleotidase family protein:conserved hypothetical protein|POSITIVE:POSITIVE|[635401,635511]|111 HSP 1 e-value: 7.0E-57 bit: 220.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 1 Subject End: 111 cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta cgctccaaaactgcttaatgctaatgccataaatgcaaataaaattcttttcatatatatttgttgttaaaacgtgctgcggaaaagtgctgataacgatgttaaagccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCUCCAAAACUGCUUAAUGCUAAUGCCAUAAAUGCAAAUAAAAUUCUUUUCAUAUAUAUUUGUUGUUAAAACGUGCUGCGGAAAAGUGCUGAUAACGAUGUUAAAGCCUA .(((.......(((.....((....)).......))).......................(((((((((....(..((.......))..))))))))))......)))... (-13.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGCUUUAACAUCGUUAUCAGCACUUUUCCGCAGCACGUUUUAACAACAAAUAUAUAUGAAAAGAAUUUUAUUUGCAUUUAUGGCAUUAGCAUUAAGCAGUUUUGGAGCG ...(((.((((...))))..)))....(((((.(((..((..(((...((((((...((.......))..))))))...)))..))....((.....)).))).))))).. (-14.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 320 108 74 194 156 pint:PI0468|5end_50S_ribosomal_protein_L29_436905..437135_POSITIVE 295 102 72 81 40 pint:PI1358|5end_phospho-N-acetylmuramoyl-pentapeptide-transferase__1274327..1274557_POSITIVE 272 110 71 165 125 pint:PI1824|5end_ThiJ/PfpI_family_protein_1764479..1764709_POSITIVE 269 43 1 55 17 pint:PI1906|5end_zinc_protease,_immunoreactive_106_kDa_antigen_PG115_1848008..1848238_POSITIVE 267 109 62 70 18 pint:PI1890|5end_cell_division_protein_FtsW_1832886..1833116_POSITIVE 265 109 62 109 70 pint:PI2169|5end_hypothetical_protein_2111706..2111936_POSITIVE 265 53 18 120 81 pint:PI1834|5end_anaerobic_C4-dicarboxylate_membrane_transporter_1774861..1775091_POSITIVE 264 100 51 104 57 pint:PI0309|5end_hypothetical_protein_291503..291733_POSITIVE 262 109 61 219 174 pint:PI2100|5end_conserved_hypothetical_protein_2053351..2053581_POSITIVE 252 109 80 67 36 pint:PI1273|5end_anthranilate_synthase_component_II_1192285..1192515_POSITIVE 250 110 73 143 102 pint:PI1511|5end_hypothetical_protein_1429580..1429810_POSITIVE 250 110 73 92 51 pint:PI1512|5end_hypothetical_protein_1429529..1429759_POSITIVE 249 109 65 202 167 pint:PI1446|5end_conserved_hypothetical_protein_1369027..1369257_POSITIVE 249 36 1 63 27 pint:PI1300|5end_probable_glycosyltransferase_1213190..1213420_POSITIVE 249 37 2 45 8 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 246 109 61 193 147 pint:PI0051|5end_hypothetical_protein_52607..52837_POSITIVE 244 39 1 112 77 pint:PI1475|5end_possible_short_chain_dehydrogenase_1397142..1397372_POSITIVE 243 109 62 195 138 pint:PI2166|5end_hypothetical_protein_2111394..2111624_POSITIVE 243 109 62 87 30 pint:PI2168|5end_hypothetical_protein_2111286..2111516_POSITIVE 243 50 1 147 108 pint:PI1313|5end_possible_POT_family_(proton-dependent_oligopeptide_transport_family)_1229710..1229940_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 272 110 64 133 95 pint:PI2160|3end_hypothetical_protein_2108357..2108507_POSITIVE 265 109 62 69 30 pint:PI2168|3end_hypothetical_protein_2111746..2111896_POSITIVE 264 100 51 123 76 pint:PI0308|3end_conserved_hypothetical_protein_291602..291752_POSITIVE 262 109 61 65 20 pint:PI2099|3end_D-alanyl-D-alanine_dipeptidase_2053277..2053427_POSITIVE 261 110 74 129 94 pint:PI0670|3end_hypothetical_protein_611280..611430_POSITIVE 252 110 72 133 98 pint:PI1922|3end_hypothetical_protein_1865454..1865604_POSITIVE 252 109 80 44 13 pint:PI1272|3end_possible_transcriptional_regulator_1192342..1192492_POSITIVE 249 36 1 44 8 pint:PI1299|3end_glycosyltransferase_1213251..1213401_POSITIVE 242 109 64 118 79 pint:PI1506|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_(queuine_synthetase)_1426562..1426712_POSITIVE 240 103 62 135 96 pint:PI1320|3end_conserved_hypothetical_protein_1239761..1239911_POSITIVE 240 110 72 129 94 pint:PI0004|3end_hypothetical_protein_6389..6539_POSITIVE 236 100 60 71 34 pint:PI1888|3end_UDP-N-acetylmuramate--L-alanine_ligase_1831475..1831625_POSITIVE 236 108 71 80 38 pint:PI1380|3end_conserved_hypothetical_protein_1303242..1303392_POSITIVE 236 110 63 103 60 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 235 109 62 135 78 pint:PI0049|3end_outer_membrane_receptor_protein_(iron_transport)_51090..51240_POSITIVE 234 109 62 142 96 pint:PI1490|3end_GTP_cyclohydrolase_I_1409127..1409277_POSITIVE 232 109 65 144 92 pint:PI1381|3end_conserved_hypothetical_protein_1303676..1303826_POSITIVE 227 108 70 51 12 pint:PI0624|3end_hypothetical_protein_574471..574621_POSITIVE 223 110 76 129 94 pint:PI1340|3end_hypothetical_protein_1256872..1257022_POSITIVE 222 79 44 146 118 pint:PI1513|3end_conserved_hypothetical_protein_1432502..1432652_POSITIVE