Origin IGS: aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacctttttggaataataagataggtgttccaaataattatataaacacgttataagcacaccccatatattgattccgttgcaaaagaaacgttaccaatgccccatacaagcactcgaaacaagaaaaagcacggtttagaaaccaattacgcccgtgaaaattaaattatctaagcttagagaaataattatctaaactttgggaagtatttatttaagcttagagaaaattttattaaagctttgataattaaaaaatataagtcagaaatcgcattatatacggcttcccgtgaacgtggaacctattaacaataatatcggaaccattgcaagcacacatatcagcgtcgaaagcaagaatacaacttactgattagcaatctaacacaggagtagttttaccaaaatctctattatataagtatgtataaggacaaatattaacaaaaaatgtaggagcaaaactttggcatttggcatatttgcgctatctttgcaatctaaggcagtacttcggcactgtcgctggtgttgcaagacacgagaggaaagtccgggcagcaaagagtatcctgcttccgaaaatgaaagctattggcaacagtagatgtaggcagaagaaaacaaccgccttggcaacgaggcaagggtgagaaggtagtgtaagagactaccagcacgtaggcgactgcgtggctgtgccgttcaggagctgcaagttcatgtaaaccgttgtttgagggttactcgcccgagccatacacggcacaacggagggtagaacgctaaaacgcacggggcgaccctgcggttagatagatgacaggcgtccttacttgtcgaaagacggcaaggaaacaagacccggcttatagaagtactgttttacacatactcgctgcctcccccaccgtggggagggagttgaagtatgggaaaggtgctgaaccacacttaaaggtttcaatacagcatcgcagaacaagcagattgtagaaccattccttgtttttt Mask Tandem Repeat Region ================================================ aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Find is-nt database================================================ Query_seq: PI0673:PI0675|PI0673:PI0675:hypothetical protein:hypothetical protein:->->:612766..613786 1021 aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0673:PI0675|PI0673:PI0675:hypothetical protein:hypothetical protein:->->:612766..613786 1021 aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0673:PI0675|PI0673:PI0675:hypothetical protein:hypothetical protein:->->:612766..613786 1021 aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 26 End: 142 ......................... M L V L R C C I E T F K C G S A P F P Y F N S L P T V G E A A S M C K T V L L ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: + Start: 24 End: 179 ....................... M C L F C D A V L K P L S V V Q H L S H T S T P S P R W G R Q R V C V K Q Y F Y K P G L V S L P S F D K .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: + Start: 142 End: 291 ............................................................................................................................................. M S R V L F P C R L S T S K D A C H L S N R R V A P C V L A F Y P P L C R V W L G R V T L K Q R F T .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 41 Strand: + Start: 219 End: 341 .......................................................................................................................................................................................................................... M R F S V L P S V V P C M A R A S N P Q T T V Y M N L Q L L N G T A T Q S P T C W ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 59 Strand: + Start: 335 End: 511 .............................................................................................................................................................................................................................................................................................................................................. M L V V S Y T T F S P L P R C Q G G C F L L P T S T V A N S F H F R K Q D T L C C P D F P L V S C N T S D S A E V L P .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 38 Strand: + Start: 411 End: 524 .......................................................................................................................................................................................................................................................................................................................................................................................................................... M L P I A F I F G S R I L F A A R T F L S C L A T P A T V P K Y C L R L Q R ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 451 End: 630 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M L P G L S S R V L Q H Q R Q C R S T A L D C K D S A N M P N A K V L L L H F L L I F V L I H T Y I I E I L V K L L L C ....................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 654 End: 764 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M L A F D A D M C A C N G S D I I V N R F H V H G K P Y I M R F L T Y I F ................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: + Start: 872 End: 967 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M V S K P C F F L F R V L V W G I G N V S F A T E S I Y G V C L ...................................................... Protein_Len: 32 Strand: - Start: 856 End: 951 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... N E R A Y N T E L G H K K K N R T S T H P M P L T E K A V S D M ...................................................................... Protein_Len: 38 Strand: - Start: 645 End: 758 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Y T T N K S E V S I H T S A I T G I N N N I P E V N V P L R I Y H S K Q S M ....................................................................................................................................................................................................................................................................... Protein_Len: 59 Strand: - Start: 411 End: 587 .......................................................................................................................................................................................................................................................................................................................................................................................................................... Q Q W Y S E N E S A P Y E K S G P S E E R T K C C W R C H R L V A K S Q L S L A F I G F A L T K S R C K K N I N T R M .................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 365 End: 577 ............................................................................................................................................................................................................................................................................................................................................................................ Q R C R S N G I A K M K P L L I S K A A R V K R E H R A V G A V T G F Y Q R L N C L Y R L Y A L H W L K A G V N K T L M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 30 Strand: - Start: 237 End: 326 ............................................................................................................................................................................................................................................ G G N H R T H S P R T V R L C R N V H V Q L E Q V A C G R M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 229 End: 318 .................................................................................................................................................................................................................................... R E V R R Q A T Y P E P S Y G E F L P K C S S A A G S R C M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 58 Strand: - Start: 22 End: 195 ..................... L R S T R R H Q I S V K L H P E A G K G Y K L E R G V T P S A A L I H L V T S R Y A P D Q K R A T K R C T L V G T M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 71 HP_score: -6.7 Tail_Score: -4.21936 Start: 205 End: 223 Full_Region: TGTCATCTATCTAAC CGCAGGG TCGC CCCGTGCG TTTTAGCGTTCTACC ............................................................................................................................................................................................................CGCAGGGTCGCCCCGTGCG.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PI0673:PI0675|PI0673:PI0675:hypothetical protein:hypothetical protein:->->:612766..613786 1021 aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Intra-Species Hit: Count: 1 Min: 1 Max: 1021 Len: 1021 Subject: pint_PI0673_PI0675|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[612766,613786]|1021 HSP 1 e-value: 0.0 bit: 2024.0 Len: 1021 Query Start:1 Query End:1021 Subject Strand: POSITIVE Subject Start: 1 Subject End: 1021 aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta aaaaaacaaggaatggttctacaatctgcttgttctgcgatgctgtattgaaacctttaagtgtggttcagcacctttcccatacttcaactccctccccacggtgggggaggcagcgagtatgtgtaaaacagtacttctataagccgggtcttgtttccttgccgtctttcgacaagtaaggacgcctgtcatctatctaaccgcagggtcgccccgtgcgttttagcgttctaccctccgttgtgccgtgtatggctcgggcgagtaaccctcaaacaacggtttacatgaacttgcagctcctgaacggcacagccacgcagtcgcctacgtgctggtagtctcttacactaccttctcacccttgcctcgttgccaaggcggttgttttcttctgcctacatctactgttgccaatagctttcattttcggaagcaggatactctttgctgcccggactttcctctcgtgtcttgcaacaccagcgacagtgccgaagtactgccttagattgcaaagatagcgcaaatatgccaaatgccaaagttttgctcctacattttttgttaatatttgtccttatacatacttatataatagagattttggtaaaactactcctgtgttagattgctaatcagtaagttgtattcttgctttcgacgctgatatgtgtgcttgcaatggttccgatattattgttaataggttccacgttcacgggaagccgtatataatgcgatttctgacttatattttttaattatcaaagctttaataaaattttctctaagcttaaataaatacttcccaaagtttagataattatttctctaagcttagataatttaattttcacgggcgtaattggtttctaaaccgtgctttttcttgtttcgagtgcttgtatggggcattggtaacgtttcttttgcaacggaatcaatatatggggtgtgcttataacgtgtttatataattatttggaacacctatcttattattccaaaaaggtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAACAAGGAAUGGUUCUACAAUCUGCUUGUUCUGCGAUGCUGUAUUGAAACCUUUAAGUGUGGUUCAGCACCUUUCCCAUACUUCAACUCCCUCCCCACGGUGGGGGAGGCAGCGAGUAUGUGUAAAACAGUACUUCUAUAAGCCGGGUCUUGUUUCCUUGCCGUCUUUCGACAAGUAAGGACGCCUGUCAUCUAUCUAACCGCAGGGUCGCCCCGUGCGUUUUAGCGUUCUACCCUCCGUUGUGCCGUGUAUGGCUCGGGCGAGUAACCCUCAAACAACGGUUUACAUGAACUUGCAGCUCCUGAACGGCACAGCCACGCAGUCGCCUACGUGCUGGUAGUCUCUUACACUACCUUCUCACCCUUGCCUCGUUGCCAAGGCGGUUGUUUUCUUCUGCCUACAUCUACUGUUGCCAAUAGCUUUCAUUUUCGGAAGCAGGAUACUCUUUGCUGCCCGGACUUUCCUCUCGUGUCUUGCAACACCAGCGACAGUGCCGAAGUACUGCCUUAGAUUGCAAAGAUAGCGCAAAUAUGCCAAAUGCCAAAGUUUUGCUCCUACAUUUUUUGUUAAUAUUUGUCCUUAUACAUACUUAUAUAAUAGAGAUUUUGGUAAAACUACUCCUGUGUUAGAUUGCUAAUCAGUAAGUUGUAUUCUUGCUUUCGACGCUGAUAUGUGUGCUUGCAAUGGUUCCGAUAUUAUUGUUAAUAGGUUCCACGUUCACGGGAAGCCGUAUAUAAUGCGAUUUCUGACUUAUAUUUUUUAAUUAUCAAAGCUUUAAUAAAAUUUUCUCUAAGCUUAAAUAAAUACUUCCCAAAGUUUAGAUAAUUAUUUCUCUAAGCUUAGAUAAUUUAAUUUUCACGGGCGUAAUUGGUUUCUAAACCGUGCUUUUUCUUGUUUCGAGUGCUUGUAUGGGGCAUUGGUAACGUUUCUUUUGCAACGGAAUCAAUAUAUGGGGUGUGCUUAUAACGUGUUUAUAUAAUUAUUUGGAACACCUAUCUUAUUAUUCCAAAAAGGUUA ....(((((((..(((((........((((.(((.(((..(((((......((((.........))))))))).......(((((((...((.(((((((......))))))).)).))))))).))).)))))))........)))))..)))))))(((((((.(((....)))..))))))).((((...............((((((..((((((((((....(((((((........((((((((((....((((...((((((((((..........)))).......)))))))))).....))))))))))...((((((......(((..((((((.......))))))....)))...............((((((((......((((.((........((((((((..............(((((.((((((......))))))..)))))..........((((......))))..)))))))))).)))).)))))))).)))))).((((.(((((......(((((.((((((((((((......(((.....)))..((((..((..........))..))))....))))))))))))....(((.((((((..((((((....((((.((((((((((...((((((..((.(((.(((((.((((((((((((......))))))))....))))..)))))))))))))))).......))))))))))))))............(((((((.((((((..........((.(((((((((................))))))))).))..........)))))).))))))))))))).)))))).))).))))).........)))))...)))).....)))))))))))))))))...(....).....))))))...(((((.((((....((((((.((.......(((....))).......)).))))))....)))))))))....)))).. (-275.24) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCUUUUUGGAAUAAUAAGAUAGGUGUUCCAAAUAAUUAUAUAAACACGUUAUAAGCACACCCCAUAUAUUGAUUCCGUUGCAAAAGAAACGUUACCAAUGCCCCAUACAAGCACUCGAAACAAGAAAAAGCACGGUUUAGAAACCAAUUACGCCCGUGAAAAUUAAAUUAUCUAAGCUUAGAGAAAUAAUUAUCUAAACUUUGGGAAGUAUUUAUUUAAGCUUAGAGAAAAUUUUAUUAAAGCUUUGAUAAUUAAAAAAUAUAAGUCAGAAAUCGCAUUAUAUACGGCUUCCCGUGAACGUGGAACCUAUUAACAAUAAUAUCGGAACCAUUGCAAGCACACAUAUCAGCGUCGAAAGCAAGAAUACAACUUACUGAUUAGCAAUCUAACACAGGAGUAGUUUUACCAAAAUCUCUAUUAUAUAAGUAUGUAUAAGGACAAAUAUUAACAAAAAAUGUAGGAGCAAAACUUUGGCAUUUGGCAUAUUUGCGCUAUCUUUGCAAUCUAAGGCAGUACUUCGGCACUGUCGCUGGUGUUGCAAGACACGAGAGGAAAGUCCGGGCAGCAAAGAGUAUCCUGCUUCCGAAAAUGAAAGCUAUUGGCAACAGUAGAUGUAGGCAGAAGAAAACAACCGCCUUGGCAACGAGGCAAGGGUGAGAAGGUAGUGUAAGAGACUACCAGCACGUAGGCGACUGCGUGGCUGUGCCGUUCAGGAGCUGCAAGUUCAUGUAAACCGUUGUUUGAGGGUUACUCGCCCGAGCCAUACACGGCACAACGGAGGGUAGAACGCUAAAACGCACGGGGCGACCCUGCGGUUAGAUAGAUGACAGGCGUCCUUACUUGUCGAAAGACGGCAAGGAAACAAGACCCGGCUUAUAGAAGUACUGUUUUACACAUACUCGCUGCCUCCCCCACCGUGGGGAGGGAGUUGAAGUAUGGGAAAGGUGCUGAACCACACUUAAAGGUUUCAAUACAGCAUCGCAGAACAAGCAGAUUGUAGAACCAUUCCUUGUUUUUU .(((((((((((((((..........))))))))................(((...(((((........(((((.(..((((........))))..).))))).(((((((..((..((((...........(((.(((((.((..............((((.(((.(((((.((((((((((((.(((((....((........))....))))).))))))))))))...))))).))).....((((((...............))))))..)))).........((.((((((....)).))))))..............)).)))))..)))..((..........)).))))..)).......((((((......((((...........((((...(((....)))..))))........(((((((.((((((..............((((((..(((.....)))..)))))).(((....)))....)))))).....((((((((((((((.......((.((.(((((....))))).)).)).(((.((((((.((...(((((..(((((((((..........((((((....)))))).(((((((..............((((((....))))))..(((((((..((((((.......))))))..(((((((....)))))))((.((.((.....)).)).))...........((((.((....)).)))).)))))))..))).))))........)))).))))).(((((....((((.(((....)))))))((((......)))).)))))...)))))(((....))).))..(....)..).))))))))...)))))))))))))).))))))).))))(((((((......)))))))))))))..)))))))....))))).))).......)))))))...............((((((((..((.((......)).)))))))))).. (-287.66) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 480 500 451 53 7 pint:PI1379|5end_hypothetical_protein_1301817..1302047_POSITIVE 473 508 465 205 163 pint:PI1735|5end_conserved_hypothetical_protein_1653428..1653658_POSITIVE 420 350 304 231 180 pint:PI1980|5end_conserved_hypothetical_protein_1937470..1937700_POSITIVE 397 267 221 72 16 pint:PI0026|5end_two-component_system_response_regulator_29756..29986_POSITIVE 396 338 296 68 26 pint:PI0705|5end_conserved_hypothetical_protein;_possible_lipoprotein_641274..641504_POSITIVE 390 250 203 150 94 pint:PI1575|5end_LemA_protein_1501143..1501373_POSITIVE 390 500 451 175 122 pint:PI0627|5end_conserved_hypothetical_protein_575618..575848_POSITIVE 384 355 311 108 58 pint:PI1550|5end_conserved_hypothetical_protein_1467360..1467590_POSITIVE 384 338 297 50 1 pint:PI0906|5end_hypothetical_protein_835653..835883_POSITIVE 382 340 297 57 3 pint:PI0658|5end_conserved_hypothetical_protein_602187..602417_POSITIVE 381 338 291 199 157 pint:PI0171|5end_hypothetical_protein_163850..164080_POSITIVE 377 340 292 149 92 pint:PI0108|5end_L-lactate_permease_104892..105122_POSITIVE 370 248 205 64 12 pint:PI1848|5end_hypothetical_protein_1793799..1794029_POSITIVE 370 258 214 62 4 pint:PI0355|5end_probable__N-acetylmuramoyl-L-alanine_amidase_326157..326387_POSITIVE 368 243 205 58 19 pint:PI1647|5end_hypothetical_protein_1561564..1561794_POSITIVE 366 379 336 156 101 pint:PI0420|5end_hypothetical_protein_386551..386781_POSITIVE 366 379 336 142 87 pint:PI0417|5end_hypothetical_protein_386537..386767_POSITIVE 363 178 136 219 181 pint:PI1931|5end_alpha_glycan_phosphorylase,_maltodextrin_phosphorylase_1875456..1875686_POSITIVE 363 344 302 203 152 pint:PI1525|5end_CDP-diacylglycerol--serine_O-phosphatidyltransferase_1446758..1446988_POSITIVE 362 340 297 60 16 pint:PI1933|5end_hypothetical_protein_1879925..1880155_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 456 250 205 95 52 pint:PI0365|3end_hypothetical_protein_336118..336268_POSITIVE 432 227 186 128 75 pint:PI1065|3end_hypothetical_protein_983650..983800_POSITIVE 397 267 221 59 3 pint:PI0025|3end_two_component_system_sensor_histidine_kinase_29823..29973_POSITIVE 396 338 296 78 36 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 392 260 211 90 19 pint:PI0999|3end_hypothetical_protein_922812..922962_POSITIVE 388 330 283 142 70 pint:PI0258|3end_conjugative_transposon_protein,_TraG_252285..252435_POSITIVE 387 126 85 99 42 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 373 275 232 107 58 pint:PI0683|3end_hypothetical_protein_619797..619947_POSITIVE 372 225 182 150 105 pint:PI2122|3end_conserved_hypothetical_protein_2070480..2070630_POSITIVE 372 270 221 114 69 pint:PI1996|3end_hypothetical_protein_1950588..1950738_POSITIVE 370 259 213 67 12 pint:PI1807|3end_hypothetical_protein_1742481..1742631_POSITIVE 370 258 214 66 8 pint:PI0354|3end_hypothetical_protein_326241..326391_POSITIVE 365 340 296 74 26 pint:PI1734|3end_possible_carboxyl-terminal_protease-related_protein_1653829..1653979_POSITIVE 359 268 221 51 9 pint:PI1754|3end_conserved_hypothetical_protein_1682010..1682160_POSITIVE 353 355 311 50 4 pint:PI1549|3end_conserved_hypothetical_protein_1467382..1467532_POSITIVE 351 403 366 150 107 pint:PI0087|3end_conserved_hypothetical_protein_86733..86883_POSITIVE 350 405 361 117 67 pint:PI2085|3end_conserved_hypothetical_protein_2041803..2041953_POSITIVE 350 330 282 55 4 pint:PI1630|3end_hypothetical_protein_1552494..1552644_POSITIVE 349 250 203 147 97 pint:PI1574|3end_conserved_hypothetical_protein_1501226..1501376_POSITIVE 348 222 184 38 2 pint:PI0355|3end_probable__N-acetylmuramoyl-L-alanine_amidase_326698..326848_POSITIVE