Origin IGS: tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcagagccaattaatgcctattcaggcgtatttcaatgattttacaaaggtaaaacaaataattcataaaaccaaacaaagaaaagattattttgttttcaaaacttttgattttaatatttaaaaacataaaaccaaacacccgcgctgtgtgcaattgcagtttgcatacacaaatataaatctacaaacctatacatacttaaagctaaaagacaacctttttacgtataatgctggttgttaatagtactttacagctaaactataaggtttatgcagaatattctttgttttatcgtggatatttcctctttttgtcgcagataggttaatgtaatattataaactactggttattaaataattaaagccttatgaaatacgatgaatatttaaacgtttacaaataagtaatctttaatttagctttgtatttacaaatacaaaacaaagtgtgcgctttttaatattcaaaacatggctattcaaatataaaaacaaagagttttattttcaaaagtaaacgtattatttaacatactaaatgttaatataagataacgctaaatctactgctttgaaaaaggactaaaaatggcgtaattttagtatttcggagcaaagtaaaggttgatttcaaataagctaaccacagaggagataaaactgtgtaagttacctattattaagcaattaagcgaaaaaagtagataaaatgagctgctttttttagctctaaaaagtacgaaaaaacggcattttttggcattttacccactaaaaacgtccattttgaggtattggaagcctcaaaatggacgtttttagttttatttctgacactaaatctatgcttttttca Mask Tandem Repeat Region ================================================ tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Find is-nt database================================================ Query_seq: PI0662:PI0663|PI0662:PI0663:conserved hypothetical protein:possible transcriptional regulator:->->:605274..606138 865 tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0662:PI0663|PI0662:PI0663:conserved hypothetical protein:possible transcriptional regulator:->->:605274..606138 865 tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0662:PI0663|PI0662:PI0663:conserved hypothetical protein:possible transcriptional regulator:->->:605274..606138 865 tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 85 End: 174 .................................................................................... M G K M P K N A V F S Y F L E L K K A A H F I Y F F R L I A ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 208 End: 312 ............................................................................................................................................................................................................... M W L A Y L K S T F T L L R N T K I T P F L V L F Q S S R F S V I L Y ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 530 End: 619 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M N L S A T K R G N I H D K T K N I L H K P Y S L A V K Y Y ...................................................................................................................................................................................................................................................... Protein_Len: 57 Strand: + Start: 682 End: 852 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Y I C V C K L Q L H T A R V F G F M F L N I K I K S F E N K I I F S L F G F M N Y L F Y L C K I I E I R L N R H ............. Protein_Len: 36 Strand: - Start: 745 End: 852 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ I N F D F T K F V F Y D K R Q K T K H I I Q K V K T F D N F Y A Q I P M ............. Protein_Len: 38 Strand: - Start: 687 End: 800 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. I Q T H L S C N C V A R T N P K I N K F I L I L L K S F L I I K E K N P K M ................................................................. Protein_Len: 31 Strand: - Start: 622 End: 714 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. C G A N Y T F L N D K L K L Y T Y T Q L N I N T Y A F Q L Q M ....................................................................................................................................................... Protein_Len: 34 Strand: - Start: 534 End: 635 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... G I Q S L F L F Y G R Y F L S Y E A Y V K Y N L Q L T S N V V L M M ...................................................................................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 38 End: 142 ..................................... F R G N Q P K W Y R L I S T K L P Y F A L F H R K K T S K L A L F L M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 3 End: 95 .. F F C L N L T L F L V L F T W K S A E L V E F H V N K T P L M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 86 Start: 345 End: 374 ........................................................................................................................................................................................................................................................................................................................................................TTTGAAAATAAAACTCTTTGTTTTTATATT........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -15.4 Tail_Score: -4.7497 Start: 42 End: 78 Full_Region: AAATAAAACTAAAAA CGTCCATTTTGAGG CTTCCAATA CCTCAAAATGGACG TTTTTAGTGGGTAAA .........................................CGTCCATTTTGAGGCTTCCAATACCTCAAAATGGACG................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -17.5 Tail_Score: -5.19819 Start: 42 End: 78 Full_Region: tttacccactaaaaa cgtccattttgaggtat tgg aagcctcaaaatggacg tttttagttttattt .........................................cgtccattttgaggtattggaagcctcaaaatggacg................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 39 HP_score: -2.3 Tail_Score: -2.75863 Start: 307 End: 334 Full_Region: AGATTTAGCGTTATC TTATATTAACAT TTAGT ATGTTAAATAA TACGTTTACTTTTGA ..................................................................................................................................................................................................................................................................................................................TTATATTAACATTTAGTATGTTAAATAA................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PI0662:PI0663|PI0662:PI0663:conserved hypothetical protein:possible transcriptional regulator:->->:605274..606138 865 tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Intra-Species Hit: Count: 1 Min: 1 Max: 865 Len: 865 Subject: pint_PI0662_PI0663|conserved hypothetical protein:possible transcriptional regulator|POSITIVE:POSITIVE|[605274,606138]|865 HSP 1 e-value: 0.0 bit: 1673.0 Len: 865 Query Start:1 Query End:865 Subject Strand: POSITIVE Subject Start: 1 Subject End: 865 tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctnnnnnnngcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga tgaaaaaagcatagatttagtgtcagaaataaaactaaaaacgtccattttgaggcttccaatacctcaaaatggacgtttttagtgggtaaaatgccaaaaaatgccgttttttcgtactttttagagctaaaaaaagcagctcattttatctacttttttcgcttaattgcttaataataggtaacttacacagttttatctcctctgtggttagcttatttgaaatcaacctttactttgctccgaaatactaaaattacgccatttttagtcctttttcaaagcagtagatttagcgttatcttatattaacatttagtatgttaaataatacgtttacttttgaaaataaaactctttgtttttatatttgaatagccatgttttgaatattaaaaagcgcacactttgttttgtatttgtaaatacaaagctaaattaaagattacttatttgtaaacgtttaaatattcatcgtatttcataaggctttaattatttaataaccagtagtttataatattacattaacctatctgcgacaaaaagaggaaatatccacgataaaacaaagaatattctgcataaaccttatagtttagctgtaaagtactattaacaaccagcattatacgtaaaaaggttgtcttttagctttaagtatgtataggtttgtagatttatatttgtgtatgcaaactgcaattgcacacagcgcgggtgtttggttttatgtttttaaatattaaaatcaaaagttttgaaaacaaaataatcttttctttgtttggttttatgaattatttgttttacctttgtaaaatcattgaaatacgcctgaataggcattaattggctctga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAAAGCAUAGAUUUAGUGUCAGAAAUAAAACUAAAAACGUCCAUUUUGAGGCUUCCAAUACCUCAAAAUGGACGUUUUUAGUGGGUAAAAUGCCAAAAAAUGCCGUUUUUUCGUACUUUUUAGAGCUAAAAAAAGCAGCUCAUUUUAUCUACUUUUUUCGCUUAAUUGCUUAAUAAUAGGUAACUUACACAGUUUUAUCUCCUCUGUGGUUAGCUUAUUUGAAAUCAACCUUUACUUUGCUCCGAAAUACUAAAAUUACGCCAUUUUUAGUCCUUUUUCAAAGCAGUAGAUUUAGCGUUAUCUUAUAUUAACAUUUAGUAUGUUAAAUAAUACGUUUACUUUUGAAAAUAAAACUCUUUGUUUUUAUAUUUGAAUAGCCAUGUUUUGAAUAUUAAAAAGCGCACACUUUGUUUUGUAUUUGUAAAUACAAAGCUAAAUUAAAGAUUACUUAUUUGUAAACGUUUAAAUAUUCAUCGUAUUUCAUAAGGCUUUAAUUAUUUAAUAACCAGUAGUUUAUAAUAUUACAUUAACCUAUCUGCGACAAAAAGAGGAAAUAUCCACGAUAAAACAAAGAAUAUUCUGCAUAAACCUUAUAGUUUAGCUGUAAAGUACUAUUAACAACCAGCAUUAUACGUAAAAAGGUUGUCUUUUAGCUUUAAGUAUGUAUAGGUUUGUAGAUUUAUAUUUGUGUAUGCAAACUGCAAUUGCACACAGCGCGGGUGUUUGGUUUUAUGUUUUUAAAUAUUAAAAUCAAAAGUUUUGAAAACAAAAUAAUCUUUUCUUUGUUUGGUUUUAUGAAUUAUUUGUUUUACCUUUGUAAAAUCAUUGAAAUACGCCUGAAUAGGCAUUAAUUGGCUCUGA .(((((((((((.(((.....))).........((((((((((((((((((((((.........)))))))))))))))))))))).(((.....))).....))))..))))))).......((((((((((...((((........(((((((.((.((..((......))..)).)))))))))....(((((...........)))))....))))........((((..((((((..(((..((((.(((((((.........)))))))....))))..)))))))))...(((((....((((.(((((((.....))))))))))).)))))......((((((((((....))))))))))...))))...(((.(((((((((((((...(((((........((((((((((....))))))))))...........((((......)))).))))).)))))))).(((((((((...(..((((..........(((.((((((((((((.........((((.....((((..((((((..((((((((.((..........((((((((((((..((((..........((((....((.......))...))))..((((((((..(((((((.....)))))))...))))))))))))))))))...))))))((((((((.....)))..)))))....)).)))))))).......((((((((((................))))))))))......))))))..))))..)))))))))))))))).))).........))))..)..)))))))))...)))))))).....)))))))))) (-196.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAGAGCCAAUUAAUGCCUAUUCAGGCGUAUUUCAAUGAUUUUACAAAGGUAAAACAAAUAAUUCAUAAAACCAAACAAAGAAAAGAUUAUUUUGUUUUCAAAACUUUUGAUUUUAAUAUUUAAAAACAUAAAACCAAACACCCGCGCUGUGUGCAAUUGCAGUUUGCAUACACAAAUAUAAAUCUACAAACCUAUACAUACUUAAAGCUAAAAGACAACCUUUUUACGUAUAAUGCUGGUUGUUAAUAGUACUUUACAGCUAAACUAUAAGGUUUAUGCAGAAUAUUCUUUGUUUUAUCGUGGAUAUUUCCUCUUUUUGUCGCAGAUAGGUUAAUGUAAUAUUAUAAACUACUGGUUAUUAAAUAAUUAAAGCCUUAUGAAAUACGAUGAAUAUUUAAACGUUUACAAAUAAGUAAUCUUUAAUUUAGCUUUGUAUUUACAAAUACAAAACAAAGUGUGCGCUUUUUAAUAUUCAAAACAUGGCUAUUCAAAUAUAAAAACAAAGAGUUUUAUUUUCAAAAGUAAACGUAUUAUUUAACAUACUAAAUGUUAAUAUAAGAUAACGCUAAAUCUACUGCUUUGAAAAAGGACUAAAAAUGGCGUAAUUUUAGUAUUUCGGAGCAAAGUAAAGGUUGAUUUCAAAUAAGCUAACCACAGAGGAGAUAAAACUGUGUAAGUUACCUAUUAUUAAGCAAUUAAGCGAAAAAAGUAGAUAAAAUGAGCUGCUUUUUUUAGCUCUAAAAAGUACGAAAAAACGGCAUUUUUUGGCAUUUUACCCACUAAAAACGUCCAUUUUGAGGUAUUGGAAGCCUCAAAAUGGACGUUUUUAGUUUUAUUUCUGACACUAAAUCUAUGCUUUUUUCA ((((((((((..((((((.(((((..(((((((((.........((((((((((((((((((((.....................))))).))))))))....))))))).(((((((((((((.(((..................(.(((((((((.......))))))))))...(((((...(((((((((((.......((((((.....(((((((.................)))))))..............((((((((....)))))).))............))))))..(.(((....))).)............)))))))..))))...)))))........))).)))))).))))))).....))))))))).)))))........(((((..((((.(((((........((((((((((((....)))))))...(((((....)))))................))))).............(((...((((((((((((.........(((.(((((((((((.....))))))).))))....))).....(((..((((((((((....(((((((.........))))))).)))))))))).......(((((.(((.....))).)))))..))))))))))))))).)))..))))).))))..))))).....((((((((((((((..........))))))))))).)))...................))))))..))))).........(((((((((((((((((((((((.......)))))))))))))))))))))))......)))))...................... (-193.13) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 341 740 693 176 119 pint:PI0136|5end_surface_antigen_BspA_127105..127335_POSITIVE 335 738 698 49 6 pint:PI0135|5end_internalin-related_protein_125849..126079_POSITIVE 330 738 704 128 86 pint:PI0504|5end_hypothetical_protein_464459..464689_POSITIVE 305 727 688 73 30 pint:PI0488|5end_conserved_hypothetical_protein_446906..447136_POSITIVE 302 723 689 98 57 pint:PI2022|5end_hypothetical_protein_1978631..1978861_POSITIVE 302 723 689 52 11 pint:PI2023|5end_hypothetical_protein_1978585..1978815_POSITIVE 302 723 689 160 119 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 302 723 689 84 43 pint:PI0659|5end_hypothetical_protein_603203..603433_POSITIVE 302 728 693 220 183 pint:PI0129|5end_hypothetical_protein_121709..121939_POSITIVE 294 277 244 43 11 pint:PI0817|5end_glycosyl_transferase,_group_1_family_protein_756861..757091_POSITIVE 293 726 681 78 28 pint:PI1501|5end_GTP-binding_elongation_factor_family_protein_TypA/BipA_1421424..1421654_POSITIVE 292 736 698 93 54 pint:PI2052|5end_hypothetical_protein_2008594..2008824_POSITIVE 287 730 682 66 11 pint:PI0516|5end_ferredoxin,_4Fe-4S_475864..476094_POSITIVE 286 125 84 123 81 pint:PI1280|5end_conserved_hypothetical_protein_1195965..1196195_POSITIVE 285 740 691 189 138 pint:PI1032|5end_methylmalonyl-CoA_decarboxylase,_alpha_subunit_948463..948693_POSITIVE 279 124 85 97 60 pint:PI1842|5end_conserved_hypothetical_protein_1787935..1788165_POSITIVE 277 79 38 84 42 pint:PI0913|5end_glycerate_dehydrogenase_842700..842930_POSITIVE 277 731 692 194 152 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 277 100 51 201 156 pint:PI0546|5end_hypothetical_protein_497799..498029_POSITIVE 274 735 691 121 69 pint:PI0206|5end_TPR-repeat-containing_protein_198112..198342_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 521 80 34 51 5 pint:PI0662|3end_conserved_hypothetical_protein_605213..605363_POSITIVE 309 728 689 63 30 pint:PI0963|3end_hypothetical_protein_893637..893787_POSITIVE 307 100 54 128 86 pint:PI1547|3end_hypothetical_protein_1466226..1466376_POSITIVE 289 728 682 141 81 pint:PI0136|3end_surface_antigen_BspA_128084..128234_POSITIVE 289 739 703 119 87 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE 279 124 85 51 14 pint:PI1841|3end_conserved_hypothetical_protein_1787969..1788119_POSITIVE 276 738 704 145 107 pint:PI0197|3end_acetate_kinase_189827..189977_POSITIVE 273 744 703 90 52 pint:PI0111|3end_hypothetical_protein_108265..108415_POSITIVE 271 726 683 144 103 pint:PI1830|3end_conserved_hypothetical_protein_1770361..1770511_POSITIVE 271 731 691 143 102 pint:PI1031|3end_hypothetical_protein_948507..948657_POSITIVE 268 198 152 68 19 pint:PI1194|3end_DnaJ_protein_(Hsp-40)_1113124..1113274_POSITIVE 268 853 813 92 53 pint:PI1143|3end_hypothetical_protein_1070504..1070654_POSITIVE 268 728 687 125 89 pint:PI0512|3end_conserved_hypothetical_protein_471440..471590_POSITIVE 268 725 692 146 115 pint:PI0163|3end_conserved_hypothetical_protein_158410..158560_POSITIVE 266 731 702 148 111 pint:PI0188|3end_phosphomethylpyrimidine_kinase_179670..179820_POSITIVE 262 730 692 129 87 pint:PI2112|3end_phosphopantetheine_adenylyltransferase_2063741..2063891_POSITIVE 261 100 51 144 102 pint:PI0702|3end_conserved_hypothetical_protein;_possible_tetracenomycin_C_synthesis_protein_homolog_639640..639790_POSITIVE 260 731 693 151 109 pint:PI0434|3end_possible_beta-lactamase_406890..407040_POSITIVE 259 733 696 89 49 pint:PI0681|3end_hypothetical_protein_619256..619406_POSITIVE 259 580 534 89 39 pint:PI0041|3end_hypothetical_protein_42023..42173_POSITIVE