Origin IGS: taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatatttttcacaacaataactttcacctaacaatctcatcatcagcgcattacaaaacctattgttttgcattccaaaagcgtaggttttgcaaggcaaaagagccgcttttggacggtaaaacctacactttcgcagtgccaaatcgaaattatcgtttttcttcagaattatctttacaaaacaagaacgattttcattctctatcctttctcattctgcattgtaaaacaacagtggttttgctatacaacagaggaatcaaatgggtgttcacgataaactgctactatatattcagtgctgttttctccgccaaacacctgctta Mask Tandem Repeat Region ================================================ taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Find is-nt database================================================ Query_seq: PI0652:PI0653|PI0652:PI0653:conserved hypothetical protein:hypothetical protein:->->:595853..596185 333 taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0652:PI0653|PI0652:PI0653:conserved hypothetical protein:hypothetical protein:->->:595853..596185 333 taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0652:PI0653|PI0652:PI0653:conserved hypothetical protein:hypothetical protein:->->:595853..596185 333 taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 176 End: 286 ............................................................................................................................................................................... M I S I W H C E S V G F T V Q K R L F C L A K P T L L E C K T I G F V M R ............................................... Protein_Len: 60 Strand: + Start: 124 End: 333 ........................................................................................................................... M E N E N R S C F V K I I L K K N D N F D L A L R K C R F Y R P K A A L L P C K T Y A F G M Q N N R F C N A L M M R L L Protein_Len: 60 Strand: - Start: 52 End: 282 ................................................... V I C F S F P Y L I F I T R T K Y L Y N Q L F V I I E I Q C Q S L T P K V T W F R S K Q R A F G V S K S H L V I P K T M ................................................... Protein_Len: 60 Strand: - Start: 2 End: 328 . Q L S L E S S F R Y N R N P V A F T Y T K G D L L P E K A K C F R R K Q F A F C Y T K Y H A S S S I T L H F N N N H F M ..... taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 65 HP_score: -5.5 Tail_Score: -4.13767 Start: 201 End: 251 Full_Region: TTTGGCACTGCGAAA GTGTAGGTTTTACCGTCCAAAAG CGGCT CTTTTGCCTTGCAAAACCTACGC TTTTGGAATGCAAAA ........................................................................................................................................................................................................GTGTAGGTTTTACCGTCCAAAAGCGGCTCTTTTGCCTTGCAAAACCTACGC.................................................................................. Find igs database================================================ Query_seq: PI0652:PI0653|PI0652:PI0653:conserved hypothetical protein:hypothetical protein:->->:595853..596185 333 taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Intra-Species Hit: Count: 20 Min: 1 Max: 333 Len: 333 Subject: pint_PI0652_PI0653|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[595853,596185]|333 HSP 1 e-value: 0.0 bit: 660.0 Len: 333 Query Start:1 Query End:333 Subject Strand: POSITIVE Subject Start: 1 Subject End: 333 taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta taagcaggtgtttggcggagaaaacagcactgaatatatagtagcagtttatcgtgaacacccatttgattcctctgttgtatagcaaaaccactgttgttttacaatgcagaatgagaaaggatagagaatgaaaatcgttcttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta Subject: pint_PI0962_PI0963|phosphoglycerate mutase:hypothetical protein|POSITIVE:POSITIVE|[892763,893337]|575 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 133 Query Start:146 Query End:278 Subject Strand: POSITIVE Subject Start: 143 Subject End: 275 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttg....................................................... .................................................................................................................................................gttttgtaaagataattcgttgaaaaaatgataatttcgatttgacattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttg....................................................... Subject: pint_PI0145_PI0146|secDF export membrane protein:transcription elongation factor|POSITIVE:POSITIVE|[139785,140477]|693 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 137 Query Start:146 Query End:282 Subject Strand: NEGATIVE Subject Start: 228 Subject End: 363 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................................... .................................................................................................................................................gttttgtaaagataattcatt-gaaaaacgataatttcgatttggcattgcgaaagcgtaggttttgcaatgcaaaacagccgcttttaccgtgcaaaacctacacttttggaatgcaaaacaataggttttataat................................................... HSP 2 e-value: 8.0E-7 bit: 56.0 Len: 36 Query Start:219 Query End:254 Subject Strand: POSITIVE Subject Start: 277 Subject End: 312 ..........................................................................................................................................................................................................................aaaagcggctcttttgccttgcaaaacctacgcttt............................................................................... ..........................................................................................................................................................................................................................aaaagcggctgttttgcattgcaaaacctacgcttt............................................................................... Subject: pint_PI1391_PI1392|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1311755,1312040]|286 HSP 1 e-value: 6.0E-26 bit: 119.0 Len: 136 Query Start:146 Query End:281 Subject Strand: NEGATIVE Subject Start: 53 Subject End: 188 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa.................................................... .................................................................................................................................................gttttgtaaagataattccgacgaaaaacggtaatttcgatttggcgttgcgaaagcggctgttttgcagcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa.................................................... Subject: pint_PI1563_PI1564|thiol:disulfide interchange protein dsbD:surface antigen BspA|POSITIVE:POSITIVE|[1482876,1483594]|719 HSP 1 e-value: 3.0E-25 bit: 117.0 Len: 143 Query Start:148 Query End:290 Subject Strand: NEGATIVE Subject Start: 374 Subject End: 516 ...................................................................................................................................................tttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgat........................................... ...................................................................................................................................................tttgtaaagataattctgatgaaaaatgataatttcgatttggcattgcgaaagcggctgttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttgtaatgcgttgat........................................... Subject: pint_PI1441_PI1442|hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme)|POSITIVE:POSITIVE|[1363734,1364088]|355 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 158 Query Start:146 Query End:303 Subject Strand: NEGATIVE Subject Start: 60 Subject End: 217 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgtta.............................. .................................................................................................................................................gttttgtaaagataatttacaaggaaaacggtattttcgatttgacgttgcgaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaacgcaaaacaataggttttgtaaagcgttgataacgagtttgtta.............................. HSP 2 e-value: 3.0E-9 bit: 63.9 Len: 36 Query Start:219 Query End:254 Subject Strand: POSITIVE Subject Start: 130 Subject End: 165 ..........................................................................................................................................................................................................................aaaagcggctcttttgccttgcaaaacctacgcttt............................................................................... ..........................................................................................................................................................................................................................aaaagcggctcttttgcattgcaaaacctacgcttt............................................................................... Subject: pint_PI0854_PI0855|MiaB-like tRNA modifying enzyme:hypothetical protein|POSITIVE:POSITIVE|[788060,789233]|1174 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 136 Query Start:147 Query End:282 Subject Strand: POSITIVE Subject Start: 741 Subject End: 876 ..................................................................................................................................................ttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................................... ..................................................................................................................................................ttttgtaaagaaagttcgcaagaaaaatgataatttcgatttggcgttgcgaaagcgtaggttttgcaacgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttataat................................................... HSP 2 e-value: 8.0E-7 bit: 56.0 Len: 36 Query Start:219 Query End:254 Subject Strand: NEGATIVE Subject Start: 792 Subject End: 827 ..........................................................................................................................................................................................................................aaaagcggctcttttgccttgcaaaacctacgcttt............................................................................... ..........................................................................................................................................................................................................................aaaagcggctgttttgcgttgcaaaacctacgcttt............................................................................... Subject: pint_PI1424_PI1425|probable peptidase family M48:conserved hypothetical protein|POSITIVE:POSITIVE|[1344105,1344627]|523 HSP 1 e-value: 6.0E-23 bit: 109.0 Len: 139 Query Start:145 Query End:283 Subject Strand: NEGATIVE Subject Start: 101 Subject End: 239 ................................................................................................................................................tgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatg.................................................. ................................................................................................................................................tgttttgtaaagattattcgtaagaaaaatgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacagccgcttttgccgtgtaaaacctacgcttttggaatgcaaaacaataggttttgcaatg.................................................. Subject: pint_PI1688_PI1689|possible GTPase:conserved hypothetical protein|POSITIVE:POSITIVE|[1603964,1604449]|486 HSP 1 e-value: 2.0E-20 bit: 101.0 Len: 131 Query Start:147 Query End:277 Subject Strand: NEGATIVE Subject Start: 174 Subject End: 304 ..................................................................................................................................................ttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggtttt........................................................ ..................................................................................................................................................ttttgtaaagataattcggagggaaaatgataatttcgatttgacgttgcgaaagcgtatgttttgcgatgcaaaagagccgcttttaccgtgcaaaacctacgcttttacaatgcaaaacaataggtttt........................................................ Subject: pint_PI1915_PI1916|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1859967,1860350]|384 HSP 1 e-value: 2.0E-19 bit: 97.6 Len: 137 Query Start:147 Query End:283 Subject Strand: POSITIVE Subject Start: 114 Subject End: 250 ..................................................................................................................................................ttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatg.................................................. ..................................................................................................................................................ttttgtaaaaataattctgacgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcacggtaaaagcgtatgttttacgttgcaaaacagccgcttttggaatgcaaaacaataggttttataatg.................................................. Subject: pint_PI0874_PI0875|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[810342,810747]|406 HSP 1 e-value: 2.0E-16 bit: 87.7 Len: 148 Query Start:144 Query End:291 Subject Strand: NEGATIVE Subject Start: 177 Subject End: 324 ...............................................................................................................................................ttgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatg.......................................... ...............................................................................................................................................ttgttttgtaaagataattcccttaaaaaaggataactgcgctttggcattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaacacgttttataacacgctgatg.......................................... Subject: pint_PI1981_PI1982|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1938199,1938578]|380 HSP 1 e-value: 1.0E-14 bit: 81.8 Len: 137 Query Start:146 Query End:282 Subject Strand: POSITIVE Subject Start: 143 Subject End: 279 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................................... .................................................................................................................................................gttttgtaaagataattctgataaaaaacagcaatttcggtttgacattgcgaaagcggctgttttgcatttcaaaacagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggttttataat................................................... Subject: pint_PI0023_PI0024|internalin-related protein:hypothetical protein|POSITIVE:POSITIVE|[28131,28517]|387 HSP 1 e-value: 6.0E-14 bit: 79.8 Len: 132 Query Start:146 Query End:277 Subject Strand: POSITIVE Subject Start: 214 Subject End: 345 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggtttt........................................................ .................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt........................................................ Subject: pint_PI0574_PI0575|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[519505,519700]|196 HSP 1 e-value: 2.0E-13 bit: 77.8 Len: 59 Query Start:145 Query End:203 Subject Strand: POSITIVE Subject Start: 8 Subject End: 66 ................................................................................................................................................tgttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtg.................................................................................................................................. ................................................................................................................................................tgttttgtaaagataattccgaagaaaaatgataatttcgatttgacgttgcgaaagtg.................................................................................................................................. HSP 2 e-value: 2.0E-10 bit: 67.9 Len: 42 Query Start:249 Query End:290 Subject Strand: POSITIVE Subject Start: 112 Subject End: 153 ........................................................................................................................................................................................................................................................cgcttttggaatgcaaaacaataggttttgtaatgcgctgat........................................... ........................................................................................................................................................................................................................................................cgcttttgcaatgcaaaacaataggttttgtaatgcgttgat........................................... Subject: pint_PI2051_PI2052|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2007896,2008733]|838 HSP 1 e-value: 3.0E-12 bit: 73.8 Len: 85 Query Start:249 Query End:333 Subject Strand: NEGATIVE Subject Start: 276 Subject End: 360 ........................................................................................................................................................................................................................................................cgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta ........................................................................................................................................................................................................................................................cgcttttaggatgcaaaacaataggttttgtaacgcactgatgattaggctgttatgcgaaaatttttcttgtgaaaaatattta HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 58 Query Start:132 Query End:189 Subject Strand: NEGATIVE Subject Start: 420 Subject End: 477 ...................................................................................................................................tgaaaatcgttcttgttttgtaaagataattc-tgaagaaaaacgataatttcgatttg................................................................................................................................................ ...................................................................................................................................tgaaaatcgctttggttttgtaaagataattcgttaagaaaa-cgataatttcgatttg................................................................................................................................................ Subject: pint_PI0948_PI0949|hypothetical protein:endonuclease|POSITIVE:POSITIVE|[882039, 882403]|365 HSP 1 e-value: 3.0E-12 bit: 73.8 Len: 93 Query Start:241 Query End:333 Subject Strand: POSITIVE Subject Start: 235 Subject End: 327 ................................................................................................................................................................................................................................................aaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta ................................................................................................................................................................................................................................................aaaacctacgcttttacaatgcaaaacaataggttttgcattgcgttgataatgagaatgttatacaataggtattcttgtgaaaaatattta HSP 2 e-value: 3.0E-9 bit: 63.9 Len: 56 Query Start:146 Query End:201 Subject Strand: POSITIVE Subject Start: 140 Subject End: 195 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaag.................................................................................................................................... .................................................................................................................................................gttttgtaaggataattcgtaaggaaaatgataatttcgatttggcattgcgaaag.................................................................................................................................... Subject: pint_PI0010_PI0011|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[11740,12281]|542 HSP 1 e-value: 3.0E-12 bit: 73.8 Len: 85 Query Start:249 Query End:333 Subject Strand: POSITIVE Subject Start: 152 Subject End: 236 ........................................................................................................................................................................................................................................................cgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta ........................................................................................................................................................................................................................................................cgcttttggaacgcaaaacaataggttttgtaatacgctgatggttagatagttacgtaataggtaagcttgtgaaaaatattta HSP 2 e-value: 8.0E-7 bit: 56.0 Len: 36 Query Start:220 Query End:255 Subject Strand: POSITIVE Subject Start: 101 Subject End: 136 ...........................................................................................................................................................................................................................aaagcggctcttttgccttgcaaaacctacgctttt.............................................................................. ...........................................................................................................................................................................................................................aaagcggctcttttgcgatgcaaaacctacgctttt.............................................................................. Subject: pint_PI0694_PI0695|Na+/dicarboxylate or sulfate symporter:xanthine/uracil permease family protein|POSITIVE:POSITIVE|[631238,631552]|315 HSP 1 e-value: 1.0E-11 bit: 71.9 Len: 56 Query Start:146 Query End:201 Subject Strand: POSITIVE Subject Start: 87 Subject End: 142 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaag.................................................................................................................................... .................................................................................................................................................gttttgtaaagataattccgacggaaaacggtaatttcgatttgacactgcgaaag.................................................................................................................................... HSP 2 e-value: 8.0E-10 bit: 65.9 Len: 85 Query Start:249 Query End:333 Subject Strand: POSITIVE Subject Start: 190 Subject End: 274 ........................................................................................................................................................................................................................................................cgcttttggaatgcaaaacaataggttttgtaatgcgctgatgatgagattgttaggtgaaagttattgttgtgaaaaatattta ........................................................................................................................................................................................................................................................cgcttttggaatgcaaaacaataggttttgcaaagtgttgatagtgagagagttaagtgaaagatactcttgcgaaaaatattta Subject: pint_PI1937_PI1938|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1891147,1891993]|847 HSP 1 e-value: 2.0E-10 bit: 67.9 Len: 130 Query Start:152 Query End:281 Subject Strand: POSITIVE Subject Start: 507 Subject End: 636 .......................................................................................................................................................taaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa.................................................... .......................................................................................................................................................taaagataattctgttaaaaagcgataactgcgctttggcgttgcgaaagcgtaacttttgcacgccaaaagagccgcttttaccttgcaaaacctacgcttttagaacgtaaaacaataggctttgtaa.................................................... Subject: pint_PI1051_PI1052|hypothetical protein:fructose-1, 6-bisphosphatase|POSITIVE:POSITIVE|[970289,970631]|343 HSP 1 e-value: 8.0E-10 bit: 65.9 Len: 33 Query Start:249 Query End:281 Subject Strand: NEGATIVE Subject Start: 37 Subject End: 69 ........................................................................................................................................................................................................................................................cgcttttggaatgcaaaacaataggttttgtaa.................................................... ........................................................................................................................................................................................................................................................cgcttttggaatgcaaaacaataggttttgtaa.................................................... HSP 2 e-value: 3.0E-9 bit: 63.9 Len: 44 Query Start:146 Query End:189 Subject Strand: NEGATIVE Subject Start: 129 Subject End: 172 .................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttg................................................................................................................................................ .................................................................................................................................................gttttgtaaagataattctgatgaaaaacaataatttcgttttg................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCAGGUGUUUGGCGGAGAAAACAGCACUGAAUAUAUAGUAGCAGUUUAUCGUGAACACCCAUUUGAUUCCUCUGUUGUAUAGCAAAACCACUGUUGUUUUACAAUGCAGAAUGAGAAAGGAUAGAGAAUGAAAAUCGUUCUUGUUUUGUAAAGAUAAUUCUGAAGAAAAACGAUAAUUUCGAUUUGGCACUGCGAAAGUGUAGGUUUUACCGUCCAAAAGCGGCUCUUUUGCCUUGCAAAACCUACGCUUUUGGAAUGCAAAACAAUAGGUUUUGUAAUGCGCUGAUGAUGAGAUUGUUAGGUGAAAGUUAUUGUUGUGAAAAAUAUUUA ..(((.((((((((.(((...((((.((((((......)))).)).)))).)))))))))))((((((((..(((.(((((((.((((((((..(((((((((.(..(((((((..((((..(((...........))).))))..))))))).(((....)))...).)))))))))...........(((.(.((((((((((((((((.(....((((((.....))))))....).)))))))))))))))).).))).........)))))))).))))...)))...)))...))))))))...))).................... (-85.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAUUUUUCACAACAAUAACUUUCACCUAACAAUCUCAUCAUCAGCGCAUUACAAAACCUAUUGUUUUGCAUUCCAAAAGCGUAGGUUUUGCAAGGCAAAAGAGCCGCUUUUGGACGGUAAAACCUACACUUUCGCAGUGCCAAAUCGAAAUUAUCGUUUUUCUUCAGAAUUAUCUUUACAAAACAAGAACGAUUUUCAUUCUCUAUCCUUUCUCAUUCUGCAUUGUAAAACAACAGUGGUUUUGCUAUACAACAGAGGAAUCAAAUGGGUGUUCACGAUAAACUGCUACUAUAUAUUCAGUGCUGUUUUCUCCGCCAAACACCUGCUUA ....................................((((.........(((..((((......))))..)))..((((((((((....((((((...))))))....)))))))))).(((((((((.....(((..((((((..(((.((((..(((((((((......((......)).......)))))))))...............))))..)))..))))))..))).......)))))))))........))))........(((((((..((..((((.((.(((........))))).))))....))...)))))))..... (-65.45) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 819 240 196 45 1 pint:PI1666|5end_conserved_hypothetical_protein_1579231..1579461_POSITIVE 752 288 241 82 35 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 734 280 231 129 80 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 734 280 231 74 25 pint:PI0200|5end_hypothetical_protein_191545..191775_POSITIVE 693 280 231 85 36 pint:PI0755|5end_hypothetical_protein_686691..686921_POSITIVE 692 290 241 161 112 pint:PI0157|5end_hypothetical_protein_151185..151415_POSITIVE 689 280 231 129 80 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 682 290 241 84 35 pint:PI2131|5end_hypothetical_protein_2080678..2080908_POSITIVE 660 270 221 229 179 pint:PI0159|5end_hypothetical_protein_151273..151503_POSITIVE 659 268 221 68 21 pint:PI1829|5end_hypothetical_protein_1769255..1769485_POSITIVE 647 283 241 231 189 pint:PI2111|5end_conserved_hypothetical_protein_2062771..2063001_POSITIVE 640 268 221 229 182 pint:PI2003|5end_hypothetical_protein_1960823..1961053_POSITIVE 640 268 221 68 21 pint:PI2002|5end_hypothetical_protein_1960662..1960892_POSITIVE 640 268 221 56 9 pint:PI1129|5end_hypothetical_protein_1055377..1055607_POSITIVE 637 290 241 112 63 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 633 250 201 76 27 pint:PI1702|5end_hypothetical_protein_1620587..1620817_POSITIVE 633 250 201 231 182 pint:PI0561|5end_hypothetical_protein_508952..509182_POSITIVE 626 280 231 102 53 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 626 280 231 63 14 pint:PI1339|5end_hypothetical_protein_1256671..1256901_POSITIVE 622 280 231 228 179 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 836 239 194 151 106 pint:PI1666|3end_conserved_hypothetical_protein_1579418..1579568_POSITIVE 658 290 241 67 18 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 653 289 244 46 1 pint:PI1391|3end_hypothetical_protein_1311694..1311844_POSITIVE 641 200 151 104 55 pint:PI0670|3end_hypothetical_protein_611280..611430_POSITIVE 640 268 221 51 4 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 640 268 221 110 63 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 640 268 221 150 103 pint:PI0689|3end_hypothetical_protein_625877..626027_POSITIVE 640 268 221 150 103 pint:PI0061|3end_hypothetical_protein_60690..60840_POSITIVE 633 250 201 109 60 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 624 267 221 149 102 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE 622 280 231 144 95 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 622 280 231 57 8 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 622 280 231 51 2 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 622 280 231 85 36 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 621 268 221 150 103 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 619 240 194 138 92 pint:PI1512|3end_hypothetical_protein_1429893..1430043_POSITIVE 606 200 151 144 95 pint:PI1167|3end_hypothetical_protein_1089890..1090040_POSITIVE 592 280 231 53 4 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 576 210 161 114 65 pint:PI2131|3end_hypothetical_protein_2080868..2081018_POSITIVE 574 308 261 55 8 pint:PI2130|3end_DNA_polymerase_I_2080711..2080861_POSITIVE