Origin IGS: taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaacaaaggtaaaacgataaaaacaggaaaacaaaaatccctgaatgctcacgcattcggggattttcctattactaacaattacactttataacataattaactcacctttcattatagcaattctctacttttctactaaattacaccattcttaaagatttgaagcgatagtcggcaatgcgacgtgaaggggcttta Mask Tandem Repeat Region ================================================ taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Find is-nt database================================================ Query_seq: PI0639:PI0640|PI0639:PI0640:small heat shock protein:conserved hypothetical protein:->->:583226..583428 203 taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0639:PI0640|PI0639:PI0640:small heat shock protein:conserved hypothetical protein:->->:583226..583428 203 taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0639:PI0640|PI0639:PI0640:small heat shock protein:conserved hypothetical protein:->->:583226..583428 203 taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 47 End: 157 .............................................. S H H L K T S F Y L I A I I F P S N I I N Y L T I T L L L F I G S H T L M .............................................. taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.9 Tail_Score: -5.52079 Start: 139 End: 164 Full_Region: TTAGTAATAGGAAAA TCCCCGAATGC GTGA GCATTCAGGGA TTTTTGTTTTCCTGT ..........................................................................................................................................TCCCCGAATGCGTGAGCATTCAGGGA....................................... TransTerm Strand: - Conf: 100 HP_score: -14.1 Tail_Score: -4.44797 Start: 139 End: 164 Full_Region: acaggaaaacaaaaa tccctgaatgc tcac gcattcgggga ttttcctattactaa ..........................................................................................................................................tccctgaatgctcacgcattcgggga....................................... Find igs database================================================ Query_seq: PI0639:PI0640|PI0639:PI0640:small heat shock protein:conserved hypothetical protein:->->:583226..583428 203 taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcagggatttttgttttcctgtttttatcgttttacctttgtttta Intra-Species Hit: Count: 1 Min: 1 Max: 164 Len: 164 Subject: pint_PI0639_PI0640|small heat shock protein:conserved hypothetical protein|POSITIVE:POSITIVE|[583226,583428]|203 HSP 1 e-value: 3.0E-88 bit: 325.0 Len: 164 Query Start:1 Query End:164 Subject Strand: POSITIVE Subject Start: 1 Subject End: 164 taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcaggga....................................... taaagccccttcacgtcgcattgccgactatcgcttcaaatctttaagaatggtgtaatttagtagaaaagtagagaattgctataatgaaaggtgagttaattatgttataaagtgtaattgttagtaataggaaaatccccgaatgcgtgagcattcaggga....................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGCCCCUUCACGUCGCAUUGCCGACUAUCGCUUCAAAUCUUUAAGAAUGGUGUAAUUUAGUAGAAAAGUAGAGAAUUGCUAUAAUGAAAGGUGAGUUAAUUAUGUUAUAAAGUGUAAUUGUUAGUAAUAGGAAAAUCCCCGAAUGCGUGAGCAUUCAGGGA .......(((((((.(..(((((.((.....))...(((.((((((....((.((.....)).))......)))))).)))...)))))..).))))..((((((..((....))..))))))........)))....((((.((((((....)))))).)))) (-35.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCCUGAAUGCUCACGCAUUCGGGGAUUUUCCUAUUACUAACAAUUACACUUUAUAACAUAAUUAACUCACCUUUCAUUAUAGCAAUUCUCUACUUUUCUACUAAAUUACACCAUUCUUAAAGAUUUGAAGCGAUAGUCGGCAAUGCGACGUGAAGGGGCUUUA (((((((((((....)))))))))))............................................(((((((.....((..(((....((((......................))))....)))))....((((......)))).)))))))...... (-32.95) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 340 37 3 231 193 pint:PI0334|5end_hypothetical_protein_313234..313464_POSITIVE 326 34 4 33 10 pint:PI0536|5end_hypothetical_protein_492163..492393_POSITIVE 293 41 5 61 9 pint:PI2158|5end_glycosyl_transferase,_group_2_family_protein_2104392..2104622_POSITIVE 290 36 3 203 152 pint:PI0529|5end_hypothetical_protein_483026..483256_POSITIVE 289 39 2 205 166 pint:PI1528|5end_hypothetical_protein_1449685..1449915_POSITIVE 287 45 3 143 93 pint:PI0541|5end_GTP-binding_protein,_GTP1/OBG_family_493760..493990_POSITIVE 268 42 5 154 103 pint:PI0088|5end_S-adenosylmethionine_synthetase_86663..86893_POSITIVE 264 33 4 59 28 pint:PI1743|5end_stationary-phase_survival_protein_1663948..1664178_POSITIVE 264 53 14 112 53 pint:PI0638|5end_hypothetical_protein_582218..582448_POSITIVE 264 38 3 152 107 pint:PI0477|5end_30S_ribosomal_protein_S5_440392..440622_POSITIVE 263 59 16 56 12 pint:PI1530|5end_conserved_hypothetical_protein_1450439..1450669_POSITIVE 261 57 13 177 132 pint:PI1802|5end_50S_ribosomal_protein_L13_1734350..1734580_POSITIVE 261 45 5 99 56 pint:PI1711|5end_ABC_transporter,_ATP-binding_protein_1627597..1627827_POSITIVE 261 55 13 85 48 pint:PI0096|5end_glycerol-3-phosphate_cytidylyltransferase_91885..92115_POSITIVE 259 35 4 229 187 pint:PI2011|5end_hypothetical_protein_1970429..1970659_POSITIVE 258 55 15 229 188 pint:PI0751|5end_conserved_hypothetical_protein_680623..680853_POSITIVE 257 27 4 179 151 pint:PI1849|5end_conserved_hypothetical_protein;_possible_acyltransferase_family_protein_1794916..1795146_POSITIVE 256 36 4 107 72 pint:PI2022|5end_hypothetical_protein_1978631..1978861_POSITIVE 256 36 4 61 26 pint:PI2023|5end_hypothetical_protein_1978585..1978815_POSITIVE 256 27 5 229 203 pint:PI1531|5end_biotin--acetyl-CoA-carboxylase_ligase_1450839..1451069_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 36 5 150 125 pint:PI1493|3end_conserved_hypothetical_protein_1412818..1412968_POSITIVE 304 33 1 149 113 pint:PI0726|3end_hypothetical_protein_659253..659403_POSITIVE 294 37 5 127 103 pint:PI1227|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148974..1149124_POSITIVE 271 28 3 150 118 pint:PI0540|3end_conserved_hypothetical_protein_493865..494015_POSITIVE 268 42 5 144 93 pint:PI0087|3end_conserved_hypothetical_protein_86733..86883_POSITIVE 264 38 3 142 97 pint:PI0476|3end_50S_ribosomal_protein_L18_440462..440612_POSITIVE 255 35 4 143 110 pint:PI0750|3end_zinc_protease_680633..680783_POSITIVE 254 34 5 50 23 pint:PI1924|3end_cytidine_deaminase_1866495..1866645_POSITIVE 250 53 16 131 98 pint:PI0492|3end_hypothetical_protein_452458..452608_POSITIVE 247 33 3 49 16 pint:PI1943|3end_hypothetical_protein_1902206..1902356_POSITIVE 246 30 3 54 25 pint:PI1848|3end_hypothetical_protein_1795015..1795165_POSITIVE 244 46 6 109 55 pint:PI0215|3end_hypothetical_protein_209004..209154_POSITIVE 243 159 130 105 72 pint:PI0983|3end_hypothetical_protein_909991..910141_POSITIVE 242 37 3 142 104 pint:PI1979|3end_conserved_hypothetical_protein_1937327..1937477_POSITIVE 239 38 7 44 11 pint:PI0091|3end_hypothetical_protein_89774..89924_POSITIVE 237 36 9 47 11 pint:PI1531|3end_biotin--acetyl-CoA-carboxylase_ligase_1451734..1451884_POSITIVE 237 36 4 118 79 pint:PI0056|3end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_55907..56057_POSITIVE 234 53 18 100 67 pint:PI1065|3end_hypothetical_protein_983650..983800_POSITIVE 234 155 116 78 38 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 233 33 5 50 19 pint:PI1723|3end_conserved_hypothetical_protein_1642133..1642283_POSITIVE