Origin IGS: tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agcatctttacccacatttcgtgtgtcaggtacctgctttgcaagtacaacaattttcaaactcatattatataatgtgttgtttagatactcgttgtttaattaaattcgtgtagcaaaagtacgatttttttataaatcaaacaaatttaatgcacaaaaaccgacaaaacgtgcacaaacaagggtatttgcgcaatgataaaagctatattatatgaatcgttttctttgctgcaacctgtttatatca Mask Tandem Repeat Region ================================================ tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Find is-nt database================================================ Query_seq: PI0615:PI0616|PI0615:PI0616:hydrolase, haloacid dehalogenase-like family:electron transfer flavoprotein, beta-subunit:->->:565447..565699 253 tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0615:PI0616|PI0615:PI0616:hydrolase, haloacid dehalogenase-like family:electron transfer flavoprotein, beta-subunit:->->:565447..565699 253 tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0615:PI0616|PI0615:PI0616:hydrolase, haloacid dehalogenase-like family:electron transfer flavoprotein, beta-subunit:->->:565447..565699 253 tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 141 End: 233 ............................................................................................................................................ V F K I L C R T D L C C M I Y Y S N S F Q Q V Q L A P V Q C M .................... Protein_Len: 31 Strand: - Start: 55 End: 147 ...................................................... Q A F V R T Q A R K T P K Q A N F K N S K Y F F R V K A V R M .......................................................................................................... tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0615:PI0616|PI0615:PI0616:hydrolase, haloacid dehalogenase-like family:electron transfer flavoprotein, beta-subunit:->->:565447..565699 253 tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Intra-Species Hit: Count: 1 Min: 1 Max: 253 Len: 253 Subject: pint_PI0615_PI0616|hydrolase, haloacid dehalogenase-like family:electron transfer flavoprotein, beta-subunit|POSITIVE:POSITIVE|[565447,565699]|253 HSP 1 e-value: 1.0E-128 bit: 460.0 Len: 253 Query Start:1 Query End:253 Subject Strand: POSITIVE Subject Start: 1 Subject End: 253 tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttatnnnnnnntcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct tgatataaacaggttgcagcaaagaaaacgattcatataatatagcttttatcattgcgcaaatacccttgtttgtgcacgttttgtcggtttttgtgcattaaatttgtttgatttataaaaaaatcgtacttttgctacacgaatttaattaaacaacgagtatctaaacaacacattatataatatgagtttgaaaattgttgtacttgcaaagcaggtacctgacacacgaaatgtgggtaaagatgct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUAUAAACAGGUUGCAGCAAAGAAAACGAUUCAUAUAAUAUAGCUUUUAUCAUUGCGCAAAUACCCUUGUUUGUGCACGUUUUGUCGGUUUUUGUGCAUUAAAUUUGUUUGAUUUAUAAAAAAAUCGUACUUUUGCUACACGAAUUUAAUUAAACAACGAGUAUCUAAACAACACAUUAUAUAAUAUGAGUUUGAAAAUUGUUGUACUUGCAAAGCAGGUACCUGACACACGAAAUGUGGGUAAAGAUGCU .........(((((.(((((((.......(((((((((.((((((..........((((((((((....)))))))))).((((.(..(.((.((((..(((((((((((.((((((......))))))............)))))))))))...)))).)).)..).))))......)))))).)))))))))......))))))))))))...((((..(((((.(((.......))))))))....)))) (-55.54) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCAUCUUUACCCACAUUUCGUGUGUCAGGUACCUGCUUUGCAAGUACAACAAUUUUCAAACUCAUAUUAUAUAAUGUGUUGUUUAGAUACUCGUUGUUUAAUUAAAUUCGUGUAGCAAAAGUACGAUUUUUUUAUAAAUCAAACAAAUUUAAUGCACAAAAACCGACAAAACGUGCACAAACAAGGGUAUUUGCGCAAUGAUAAAAGCUAUAUUAUAUGAAUCGUUUUCUUUGCUGCAACCUGUUUAUAUCA .............(((...((.(((((..((((.(((...))).))))..........((((.((((((....))))))..)))).))))).)).))).....(((((...(((((((((...(((((((...(((((...........(((.(((.((((.(((.....................))).)))).))).)))...........))))).)))))))....)))))))))....)))))..... (-50.55) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 330 100 51 167 122 pint:PI0651|5end_HD_superfamily_hydrolase_592270..592500_POSITIVE 287 96 55 106 62 pint:PI1142|5end_S1_RNA_binding_domain_protein,_S1_ribosomal_protein_1069892..1070122_POSITIVE 281 99 57 64 20 pint:PI2126|5end_conserved_hypothetical_protein_2074907..2075137_POSITIVE 280 243 201 54 15 pint:PI1888|5end_UDP-N-acetylmuramate--L-alanine_ligase_1829980..1830210_POSITIVE 280 235 203 229 190 pint:PI0546|5end_hypothetical_protein_497799..498029_POSITIVE 279 100 53 206 160 pint:PI1180|5end_ferric_uptake_regulation_protein_1101892..1102122_POSITIVE 271 100 58 100 55 pint:PI0133|5end_hypothetical_protein_124334..124564_POSITIVE 269 99 53 219 172 pint:PI1383|5end_biopolymer_transport_protein_tolQ_related_1304121..1304351_POSITIVE 268 244 201 115 72 pint:PI1876|5end_possible_flagellar_motor_protein,_OmpA_family_1819082..1819312_POSITIVE 268 90 42 160 104 pint:PI0129|5end_hypothetical_protein_121709..121939_POSITIVE 262 98 51 82 32 pint:PI0839|5end_conserved_hypothetical_protein_773843..774073_POSITIVE 262 100 54 144 92 pint:PI0833|5end_conserved_hypothetical_protein_770059..770289_POSITIVE 262 94 58 225 197 pint:PI0496|5end_heat_shock_protein_hsp70_(chaperone_protein_dnaK)_456632..456862_POSITIVE 261 246 202 161 106 pint:PI0721|5end_hypothetical_protein_654615..654845_POSITIVE 260 247 211 70 37 pint:PI1617|5end_hypothetical_protein_1542923..1543153_POSITIVE 260 100 53 225 170 pint:PI1607|5end_ABC-related_lipoprotein_releasing_system,_ATP-binding_protein_1535125..1535355_POSITIVE 260 100 53 199 144 pint:PI1606|5end_conserved_hypothetical_protein_1535099..1535329_POSITIVE 260 103 67 50 19 pint:PI1514|5end_tRNA_nucleotidyltransferase/poly(A)_polymerase_1432544..1432774_POSITIVE 259 99 52 117 71 pint:PI0528|5end_hypothetical_protein_482791..483021_POSITIVE 257 104 68 145 107 pint:PI2014|5end_conserved_hypothetical_protein_1971471..1971701_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 324 82 45 137 107 pint:PI0304|3end_hypothetical_protein_289705..289855_POSITIVE 319 229 185 57 15 pint:PI0059|3end_peptide_chain_release_factor_RF-2_58171..58321_POSITIVE 286 243 201 134 96 pint:PI2093|3end_hypothetical_protein_2049440..2049590_POSITIVE 286 243 201 100 62 pint:PI2092|3end_hypothetical_protein_2049406..2049556_POSITIVE 281 99 57 56 12 pint:PI2125|3end_D-tyrosyl-tRNA(Tyr)_deacylase_2074979..2075129_POSITIVE 279 100 53 57 11 pint:PI1179|3end_adenylosuccinate_synthetase_1101823..1101973_POSITIVE 276 99 53 128 86 pint:PI1527|3end_immunoreactive_46_kDa_antigen_PG99_1449520..1449670_POSITIVE 268 244 201 59 16 pint:PI1875|3end_hypothetical_protein_1819106..1819256_POSITIVE 268 96 51 123 67 pint:PI1143|3end_hypothetical_protein_1070504..1070654_POSITIVE 263 247 201 99 49 pint:PI0227|3end_ABC_transporter,_ATP-binding/permease_protein_225632..225782_POSITIVE 262 98 51 86 36 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE 259 100 53 66 26 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 259 100 51 123 71 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE 257 104 68 149 111 pint:PI2012|3end_hypothetical_protein_1971555..1971705_POSITIVE 254 100 55 142 97 pint:PI0981|3end_outer_membrane_protein,_TonB_dependent_receptor_909390..909540_POSITIVE 250 96 57 107 63 pint:PI1740|3end_DedA_family_protein_1662316..1662466_POSITIVE 249 99 55 142 78 pint:PI0570|3end_exsB_protein;_possible_aluminum_resistance_protein_515395..515545_POSITIVE 248 110 66 125 68 pint:PI0052|3end_hypothetical_protein_52715..52865_POSITIVE 248 110 66 81 24 pint:PI0050|3end_conserved_hypothetical_protein_52671..52821_POSITIVE 245 100 52 132 76 pint:PI1586|3end_possible_HesA/MoeB/ThiF_family_protein_1515183..1515333_POSITIVE