Origin IGS: taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttttaccttttataaattaacgatacaaagataagcgataacttcttatatcgtcaaagtatttgtgttaaattcttctaatcttataaaagttatagcaaacaaccgccaatacagccgatatgtctgccactacacaatatttatatatggttta Mask Tandem Repeat Region ================================================ taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Find is-nt database================================================ Query_seq: PI0601:PI0602|PI0601:PI0602:hypothetical protein:conserved hypothetical protein:->->:549081..549238 158 taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0601:PI0602|PI0601:PI0602:hypothetical protein:conserved hypothetical protein:->->:549081..549238 158 taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0601:PI0602|PI0601:PI0602:hypothetical protein:conserved hypothetical protein:->->:549081..549238 158 taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: - Start: 1 End: 120 L G Y I F I T Y H C V Y R S Y Q R N N A I V K I L N S S N L V F V K V I Y S T M ...................................... taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0601:PI0602|PI0601:PI0602:hypothetical protein:conserved hypothetical protein:->->:549081..549238 158 taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 158 Len: 158 Subject: pint_PI0601_PI0602|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[549081,549238]|158 HSP 1 e-value: 9.0E-85 bit: 313.0 Len: 158 Query Start:1 Query End:158 Subject Strand: POSITIVE Subject Start: 1 Subject End: 158 taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa taaaccatatataaatattgtgtagtggcagacatatcggctgtattggcggttgtttgctataacttttataagattagaagaatttaacacaaatactttgacgatataagaagttatcgcttatctttgtatcgttaatttataaaaggtaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACCAUAUAUAAAUAUUGUGUAGUGGCAGACAUAUCGGCUGUAUUGGCGGUUGUUUGCUAUAACUUUUAUAAGAUUAGAAGAAUUUAACACAAAUACUUUGACGAUAUAAGAAGUUAUCGCUUAUCUUUGUAUCGUUAAUUUAUAAAAGGUAAAAA ...(((...(((((((.((((((.((((((((((.((((.(......).))))))))))))))..((((((......))))))......))))))......((((((((((((((((....))))...)))))))))))))))))))...)))..... (-43.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUACCUUUUAUAAAUUAACGAUACAAAGAUAAGCGAUAACUUCUUAUAUCGUCAAAGUAUUUGUGUUAAAUUCUUCUAAUCUUAUAAAAGUUAUAGCAAACAACCGCCAAUACAGCCGAUAUGUCUGCCACUACACAAUAUUUAUAUAUGGUUUA .....(((...(((((((....((((((((.....((((((........)))))).......))))))))......................((....(((.(((..((.(......).))...))).)))....)).....)))))))...)))... (-21.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 332 62 24 47 1 pint:PI2018|5end_50S_ribosomal_protein_L34_1975757..1975987_POSITIVE 328 64 37 92 63 pint:PI0749|5end_hypothetical_protein_677234..677464_POSITIVE 324 50 5 114 59 pint:PI1497|5end_LuxS_protein,_autoinducer_AI-2_production_protein_1417571..1417801_POSITIVE 322 69 28 87 47 pint:PI1921|5end_conserved_hypothetical_protein_1863917..1864147_POSITIVE 305 57 15 77 21 pint:PI1872|5end_glycosyl_transferase,_group_2_family_protein_1817057..1817287_POSITIVE 298 60 22 224 182 pint:PI1607|5end_ABC-related_lipoprotein_releasing_system,_ATP-binding_protein_1535125..1535355_POSITIVE 298 60 22 198 156 pint:PI1606|5end_conserved_hypothetical_protein_1535099..1535329_POSITIVE 297 54 11 167 125 pint:PI1067|5end_thymidylate_synthase_983940..984170_POSITIVE 297 60 18 217 178 pint:PI0380|5end_conserved_hypothetical_protein_350162..350392_POSITIVE 290 60 15 213 153 pint:PI2049|5end_possible_transcriptional_regulator,_AraC_family_2002502..2002732_POSITIVE 289 60 20 229 195 pint:PI1231|5end_hypothetical_protein_1153175..1153405_POSITIVE 288 62 23 188 153 pint:PI1420|5end_probable_membrane_protein_1340123..1340353_POSITIVE 286 60 25 40 3 pint:PI0128|5end_dipeptidase,_pathogenicity_island-encoded_protein_D_120532..120762_POSITIVE 283 60 20 177 130 pint:PI0542|5end_hypothetical_protein_495554..495784_POSITIVE 277 60 18 172 137 pint:PI1489|5end_exodeoxyribonuclease_III_1407758..1407988_POSITIVE 277 60 18 38 3 pint:PI1487|5end_hypothetical_protein_1407624..1407854_POSITIVE 274 53 23 28 1 pint:PI0204|5end_dGTP_triphosphohydrolase_(deoxyguanosinetriphosphate_triphosphohydrolase)_196119..196349_POSITIVE 273 60 19 67 22 pint:PI2149|5end_3-oxoacyl-acyl-carrier-protein_synthase_2095454..2095684_POSITIVE 273 69 24 177 139 pint:PI1543|5end_hypothetical_protein_1462773..1463003_POSITIVE 272 59 22 55 4 pint:PI0220|5end_conserved_hypothetical_protein_211980..212210_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 332 54 19 151 115 pint:PI1773|3end_alpha-amylase_(neopullulanase)_1706107..1706257_POSITIVE 328 55 20 39 7 pint:PI1541|3end_hypothetical_protein_1461884..1462034_POSITIVE 305 60 16 61 14 pint:PI1924|3end_cytidine_deaminase_1866495..1866645_POSITIVE 305 57 15 73 17 pint:PI1871|3end_glycosyl_transferase,_group_1_family_protein_1817133..1817283_POSITIVE 301 55 22 63 30 pint:PI0324|3end_conserved_hypothetical_protein_305680..305830_POSITIVE 297 54 11 79 37 pint:PI1066|3end_dihydrofolate_reductase_983932..984082_POSITIVE 293 64 25 87 47 pint:PI0798|3end_malonyl_CoA-acyl_carrier_protein_transacylase_736975..737125_POSITIVE 290 70 21 149 88 pint:PI1999|3end_hypothetical_protein_1956668..1956818_POSITIVE 288 62 23 148 113 pint:PI1419|3end_conserved_hypothetical_protein;_membrane_protease_subunits,_stomatin/prohibitin_homologs_1340163..1340313_POSITIVE 286 68 38 51 15 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE 284 60 15 121 68 pint:PI0681|3end_hypothetical_protein_619256..619406_POSITIVE 274 59 14 43 5 pint:PI1734|3end_possible_carboxyl-terminal_protease-related_protein_1653829..1653979_POSITIVE 273 60 19 74 29 pint:PI2148|3end_ribonuclease_III_2095541..2095691_POSITIVE 272 60 19 121 75 pint:PI0126|3end_conserved_hypothetical_protein_118589..118739_POSITIVE 271 60 20 141 86 pint:PI0671|3end_hypothetical_protein_611807..611957_POSITIVE 269 52 17 106 67 pint:PI1380|3end_conserved_hypothetical_protein_1303242..1303392_POSITIVE 268 52 20 80 43 pint:PI0286|3end_ISPg3-related_transposase_272065..272215_POSITIVE 267 55 17 113 73 pint:PI0170|3end_hypothetical_protein_163891..164041_POSITIVE 265 60 20 149 119 pint:PI2053|3end_conserved_hypothetical_protein;_possible_outer_membrane_receptor_protein_2012876..2013026_POSITIVE 261 60 15 83 28 pint:PI0654|3end_K+-dependent_Na+/Ca+_exchanger_related-protein_597223..597373_POSITIVE