Origin IGS: ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtgtccaagtgtcatctacgtgtaaattcggcaatgtttcccctatgcgttttcctgctcttttccaatgttttacgttgtttttacaacaacacgacaaattatttacgtgtaaataaatatttttctacacgtaaataataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggctgacaacaatttttgtgggtataagcctat Mask Tandem Repeat Region ================================================ ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Find is-nt database================================================ Query_seq: PI0598:PI0599|PI0598:PI0599:hypothetical protein:hypothetical protein:->->:547294..547515 222 ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0598:PI0599|PI0598:PI0599:hypothetical protein:hypothetical protein:->->:547294..547515 222 ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0598:PI0599|PI0598:PI0599:hypothetical protein:hypothetical protein:->->:547294..547515 222 ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 1 End: 117 M G L Y P Q K L L S A E L L T R K E I F I Y V Y G N Y Y L R V E K Y L F T R K ......................................................................................................... Protein_Len: 40 Strand: + Start: 101 End: 220 .................................................................................................... M Y L H V N N L S C C C K N N V K H W K R A G K R I G E T L P N L H V D D T W T .. Protein_Len: 36 Strand: - Start: 106 End: 213 ......................................................................................................... K C T F L K D H Q Q L F L T F C Q F L A P F R M P S V N G F K C T S S M ......... Protein_Len: 46 Strand: - Start: 84 End: 221 ................................................................................... K R T S F Y K N V R L Y N T T N N Y F C R L V N S F L L F V C L P F M A S N V R L H C K S M . ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.8 Tail_Score: -4.10178 Start: 81 End: 120 Full_Region: CGTGTATGGAAATTA TTATTTACGTGTAGAAAA ATAT TTATTTACACGTAAATAA TTTGTCGTGTTGTTG ................................................................................TTATTTACGTGTAGAAAAATATTTATTTACACGTAAATAA...................................................................................................... TransTerm Strand: - Conf: 77 HP_score: -9.6 Tail_Score: -2.94144 Start: 81 End: 120 Full_Region: caacaacacgacaaa ttatttacgtgtaaataa atat ttttctacacgtaaataa taatttccatacacg ................................................................................ttatttacgtgtaaataaatatttttctacacgtaaataa...................................................................................................... TransTerm Strand: - Conf: 70 HP_score: -5.0 Tail_Score: -4.56597 Start: 142 End: 156 Full_Region: tttcctgctcttttc caatgt ttt acgttg tttttacaacaacac .............................................................................................................................................caatgttttacgttg.................................................................. Find igs database================================================ Query_seq: PI0598:PI0599|PI0598:PI0599:hypothetical protein:hypothetical protein:->->:547294..547515 222 ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact Intra-Species Hit: Count: 1 Min: 1 Max: 222 Len: 222 Subject: pint_PI0598_PI0599|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[547294,547515]|222 HSP 1 e-value: 1.0E-123 bit: 440.0 Len: 222 Query Start:1 Query End:222 Subject Strand: POSITIVE Subject Start: 1 Subject End: 222 ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact ataggcttatacccacaaaaattgttgtcagccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattatttacgtgtagaaaaatatttatttacacgtaaataatttgtcgtgttgttgtaaaaacaacgtaaaacattggaaaagagcaggaaaacgcataggggaaacattgccgaatttacacgtagatgacacttggacact HSP 2 e-value: 8.0E-6 bit: 52.0 Len: 42 Query Start:80 Query End:121 Subject Strand: NEGATIVE Subject Start: 80 Subject End: 121 ...............................................................................attatttacgtgtagaaaaatatttatttacacgtaaataat..................................................................................................... ...............................................................................attatttacgtgtaaataaatatttttctacacgtaaataat..................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGGCUUAUACCCACAAAAAUUGUUGUCAGCCGAAUUACUUACACGUAAAGAAAUAUUUAUUUACGUGUAUGGAAAUUAUUAUUUACGUGUAGAAAAAUAUUUAUUUACACGUAAAUAAUUUGUCGUGUUGUUGUAAAAACAACGUAAAACAUUGGAAAAGAGCAGGAAAACGCAUAGGGGAAACAUUGCCGAAUUUACACGUAGAUGACACUUGGACACU ...((((.(((...(((.....)))))).))))...((((......))))........(((((((((((((..((....((((((((((((((((.((....)).))))))))))))))))...))..((((((.....))))))........((((......((........)).....(....)....))))...)))))))))))))............ (-51.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGUCCAAGUGUCAUCUACGUGUAAAUUCGGCAAUGUUUCCCCUAUGCGUUUUCCUGCUCUUUUCCAAUGUUUUACGUUGUUUUUACAACAACACGACAAAUUAUUUACGUGUAAAUAAAUAUUUUUCUACACGUAAAUAAUAAUUUCCAUACACGUAAAUAAAUAUUUCUUUACGUGUAAGUAAUUCGGCUGACAACAAUUUUUGUGGGUAUAAGCCUAU ..........(((((.((.(((((......((.(((((.........)))))..)).....................(((((....))))).)))))....((((((((((((((..............))))))))))))))........((((((((((.((....)).))))))))))........)).))))).........((((((....)))))) (-47.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 561 70 25 114 69 pint:PI0579|5end_hypothetical_protein_523419..523649_POSITIVE 542 90 41 134 85 pint:PI0648|5end_hypothetical_protein_589448..589678_POSITIVE 522 90 41 112 63 pint:PI1462|5end_hypothetical_protein_1385094..1385324_POSITIVE 488 70 25 205 157 pint:PI0702|5end_conserved_hypothetical_protein;_possible_tetracenomycin_C_synthesis_protein_homolog_638640..638870_POSITIVE 441 90 41 149 100 pint:PI0983|5end_hypothetical_protein_909750..909980_POSITIVE 429 90 41 156 106 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 411 70 25 92 47 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 410 60 14 104 58 pint:PI0535|5end_hypothetical_protein_489508..489738_POSITIVE 370 76 37 176 137 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 368 71 40 133 101 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 356 100 57 227 185 pint:PI0917|5end_phosphoglycolate_phosphatase_846371..846601_POSITIVE 343 216 173 59 12 pint:PI0575|5end_hypothetical_protein_519561..519791_POSITIVE 337 120 72 165 119 pint:PI0115|5end_hypothetical_protein_108062..108292_POSITIVE 337 120 72 151 105 pint:PI0111|5end_hypothetical_protein_108048..108278_POSITIVE 337 120 72 116 70 pint:PI0112|5end_hypothetical_protein_108013..108243_POSITIVE 335 129 81 219 176 pint:PI0173|5end_hypothetical_protein_165305..165535_POSITIVE 317 195 152 61 18 pint:PI0929|5end_copper_homeostasis_protein_860294..860524_POSITIVE 296 200 152 199 152 pint:PI0735|5end_ABC_transporter,_ATP-binding_/_permease_668793..669023_POSITIVE 291 71 33 115 77 pint:PI0130|5end_hypothetical_protein_122032..122262_POSITIVE 284 190 141 105 61 pint:PI0553|5end_recombination_protein_503344..503574_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 553 120 71 72 17 pint:PI0533|3end_ABC_transporter,_permease_protein_488421..488571_POSITIVE 529 120 71 50 3 pint:PI0797|3end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735752..735902_POSITIVE 468 90 41 64 19 pint:PI1314|3end_hypothetical_protein_1231423..1231573_POSITIVE 429 130 81 66 11 pint:PI0701|3end_hypothetical_protein_638537..638687_POSITIVE 405 130 81 138 88 pint:PI0725|3end_hypothetical_protein_658933..659083_POSITIVE 402 130 81 52 2 pint:PI1461|3end_conserved_hypothetical_protein_1385073..1385223_POSITIVE 389 150 103 48 1 pint:PI0491|3end_hypothetical_protein_451227..451377_POSITIVE 369 129 87 54 11 pint:PI0808|3end_ISPg3-related_transposase_746457..746607_POSITIVE 335 129 81 72 29 pint:PI0172|3end_ISPg3-related_transposase_165238..165388_POSITIVE 330 124 84 51 11 pint:PI2160|3end_hypothetical_protein_2108357..2108507_POSITIVE 296 200 152 86 39 pint:PI0734|3end_ABC_transporter,_ATP-binding_/_permease_668760..668910_POSITIVE 282 175 131 111 75 pint:PI0523|3end_hypothetical_protein_479844..479994_POSITIVE 281 193 153 146 102 pint:PI1642|3end_conserved_hypothetical_protein_1560439..1560589_POSITIVE 276 215 174 149 100 pint:PI0083|3end_tyrosyl-tRNA_synthetase_84115..84265_POSITIVE 275 210 161 82 36 pint:PI1015|3end_conserved_hypothetical_protein_937347..937497_POSITIVE 275 198 151 56 8 pint:PI0262|3end_conserved_hypothetical_protein_255094..255244_POSITIVE 274 203 163 114 73 pint:PI0056|3end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_55907..56057_POSITIVE 269 117 84 142 109 pint:PI2105|3end_hypothetical_protein_2059586..2059736_POSITIVE 269 117 84 54 21 pint:PI2106|3end_hypothetical_protein_2059498..2059648_POSITIVE 266 220 173 147 94 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE